Amino acid dipepetide frequency for Pseudonocardia sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.276AlaAla: 17.276 ± 0.461
1.035AlaCys: 1.035 ± 0.082
7.145AlaAsp: 7.145 ± 0.254
7.248AlaGlu: 7.248 ± 0.21
3.518AlaPhe: 3.518 ± 0.143
10.777AlaGly: 10.777 ± 0.239
2.952AlaHis: 2.952 ± 0.126
5.349AlaIle: 5.349 ± 0.166
2.649AlaLys: 2.649 ± 0.128
12.036AlaLeu: 12.036 ± 0.299
2.786AlaMet: 2.786 ± 0.12
2.631AlaAsn: 2.631 ± 0.139
5.44AlaPro: 5.44 ± 0.182
3.787AlaGln: 3.787 ± 0.152
8.421AlaArg: 8.421 ± 0.214
6.521AlaSer: 6.521 ± 0.242
7.03AlaThr: 7.03 ± 0.203
10.343AlaVal: 10.343 ± 0.229
1.579AlaTrp: 1.579 ± 0.105
2.134AlaTyr: 2.134 ± 0.104
0.0AlaXaa: 0.0 ± 0.0
Cys
1.127CysAla: 1.127 ± 0.09
0.08CysCys: 0.08 ± 0.019
0.509CysAsp: 0.509 ± 0.052
0.452CysGlu: 0.452 ± 0.051
0.297CysPhe: 0.297 ± 0.039
0.955CysGly: 0.955 ± 0.092
0.246CysHis: 0.246 ± 0.036
0.338CysIle: 0.338 ± 0.044
0.12CysLys: 0.12 ± 0.03
0.858CysLeu: 0.858 ± 0.075
0.172CysMet: 0.172 ± 0.034
0.212CysAsn: 0.212 ± 0.031
0.601CysPro: 0.601 ± 0.075
0.252CysGln: 0.252 ± 0.033
0.715CysArg: 0.715 ± 0.065
0.641CysSer: 0.641 ± 0.064
0.629CysThr: 0.629 ± 0.07
0.726CysVal: 0.726 ± 0.062
0.143CysTrp: 0.143 ± 0.03
0.149CysTyr: 0.149 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
6.664AspAla: 6.664 ± 0.238
0.515AspCys: 0.515 ± 0.061
4.084AspAsp: 4.084 ± 0.146
4.13AspGlu: 4.13 ± 0.16
1.831AspPhe: 1.831 ± 0.117
5.36AspGly: 5.36 ± 0.206
1.459AspHis: 1.459 ± 0.101
2.872AspIle: 2.872 ± 0.13
1.241AspLys: 1.241 ± 0.073
6.395AspLeu: 6.395 ± 0.214
0.99AspMet: 0.99 ± 0.082
1.39AspAsn: 1.39 ± 0.088
3.861AspPro: 3.861 ± 0.162
1.928AspGln: 1.928 ± 0.11
4.914AspArg: 4.914 ± 0.184
3.158AspSer: 3.158 ± 0.148
3.467AspThr: 3.467 ± 0.164
4.977AspVal: 4.977 ± 0.152
1.115AspTrp: 1.115 ± 0.074
1.361AspTyr: 1.361 ± 0.102
0.0AspXaa: 0.0 ± 0.0
Glu
5.801GluAla: 5.801 ± 0.202
0.532GluCys: 0.532 ± 0.051
2.683GluAsp: 2.683 ± 0.129
2.86GluGlu: 2.86 ± 0.15
2.002GluPhe: 2.002 ± 0.111
3.501GluGly: 3.501 ± 0.133
1.596GluHis: 1.596 ± 0.097
3.095GluIle: 3.095 ± 0.124
1.436GluLys: 1.436 ± 0.091
6.378GluLeu: 6.378 ± 0.226
1.224GluMet: 1.224 ± 0.078
1.482GluAsn: 1.482 ± 0.087
2.683GluPro: 2.683 ± 0.135
2.351GluGln: 2.351 ± 0.134
4.342GluArg: 4.342 ± 0.18
3.369GluSer: 3.369 ± 0.152
2.957GluThr: 2.957 ± 0.125
4.29GluVal: 4.29 ± 0.174
0.881GluTrp: 0.881 ± 0.085
1.453GluTyr: 1.453 ± 0.097
0.0GluXaa: 0.0 ± 0.0
Phe
3.884PheAla: 3.884 ± 0.138
0.418PheCys: 0.418 ± 0.052
2.466PheAsp: 2.466 ± 0.113
1.779PheGlu: 1.779 ± 0.099
1.018PhePhe: 1.018 ± 0.084
3.415PheGly: 3.415 ± 0.159
0.686PheHis: 0.686 ± 0.053
1.333PheIle: 1.333 ± 0.107
0.492PheLys: 0.492 ± 0.051
2.9PheLeu: 2.9 ± 0.141
0.515PheMet: 0.515 ± 0.057
0.675PheAsn: 0.675 ± 0.076
1.356PhePro: 1.356 ± 0.078
0.664PheGln: 0.664 ± 0.065
1.939PheArg: 1.939 ± 0.103
1.853PheSer: 1.853 ± 0.094
2.128PheThr: 2.128 ± 0.104
2.551PheVal: 2.551 ± 0.125
0.492PheTrp: 0.492 ± 0.057
0.721PheTyr: 0.721 ± 0.064
0.0PheXaa: 0.0 ± 0.0
Gly
9.553GlyAla: 9.553 ± 0.264
0.761GlyCys: 0.761 ± 0.07
4.874GlyAsp: 4.874 ± 0.186
4.422GlyGlu: 4.422 ± 0.159
3.106GlyPhe: 3.106 ± 0.156
7.316GlyGly: 7.316 ± 0.237
2.185GlyHis: 2.185 ± 0.119
4.582GlyIle: 4.582 ± 0.189
2.174GlyLys: 2.174 ± 0.124
7.906GlyLeu: 7.906 ± 0.237
2.225GlyMet: 2.225 ± 0.12
2.271GlyAsn: 2.271 ± 0.123
4.09GlyPro: 4.09 ± 0.163
2.729GlyGln: 2.729 ± 0.128
6.653GlyArg: 6.653 ± 0.203
5.635GlySer: 5.635 ± 0.199
5.692GlyThr: 5.692 ± 0.192
6.899GlyVal: 6.899 ± 0.185
1.636GlyTrp: 1.636 ± 0.089
2.237GlyTyr: 2.237 ± 0.121
0.0GlyXaa: 0.0 ± 0.0
His
2.568HisAla: 2.568 ± 0.133
0.269HisCys: 0.269 ± 0.04
1.379HisAsp: 1.379 ± 0.088
1.133HisGlu: 1.133 ± 0.09
0.824HisPhe: 0.824 ± 0.075
2.391HisGly: 2.391 ± 0.12
0.829HisHis: 0.829 ± 0.075
0.864HisIle: 0.864 ± 0.076
0.372HisLys: 0.372 ± 0.044
2.431HisLeu: 2.431 ± 0.114
0.498HisMet: 0.498 ± 0.051
0.549HisAsn: 0.549 ± 0.06
1.791HisPro: 1.791 ± 0.108
0.807HisGln: 0.807 ± 0.073
2.208HisArg: 2.208 ± 0.124
1.299HisSer: 1.299 ± 0.086
1.384HisThr: 1.384 ± 0.103
1.831HisVal: 1.831 ± 0.107
0.492HisTrp: 0.492 ± 0.049
0.481HisTyr: 0.481 ± 0.053
0.0HisXaa: 0.0 ± 0.0
Ile
7.065IleAla: 7.065 ± 0.19
0.469IleCys: 0.469 ± 0.048
3.941IleAsp: 3.941 ± 0.162
3.1IleGlu: 3.1 ± 0.12
1.19IlePhe: 1.19 ± 0.07
4.708IleGly: 4.708 ± 0.178
0.789IleHis: 0.789 ± 0.075
1.516IleIle: 1.516 ± 0.108
0.761IleLys: 0.761 ± 0.069
3.449IleLeu: 3.449 ± 0.153
0.698IleMet: 0.698 ± 0.065
1.281IleAsn: 1.281 ± 0.086
2.574IlePro: 2.574 ± 0.141
0.875IleGln: 0.875 ± 0.082
3.106IleArg: 3.106 ± 0.125
2.763IleSer: 2.763 ± 0.124
3.421IleThr: 3.421 ± 0.156
4.044IleVal: 4.044 ± 0.157
0.469IleTrp: 0.469 ± 0.053
0.852IleTyr: 0.852 ± 0.072
0.0IleXaa: 0.0 ± 0.0
Lys
2.706LysAla: 2.706 ± 0.139
0.143LysCys: 0.143 ± 0.026
1.093LysAsp: 1.093 ± 0.087
0.978LysGlu: 0.978 ± 0.081
0.646LysPhe: 0.646 ± 0.065
1.47LysGly: 1.47 ± 0.101
0.589LysHis: 0.589 ± 0.069
1.344LysIle: 1.344 ± 0.086
0.566LysLys: 0.566 ± 0.069
2.122LysLeu: 2.122 ± 0.11
0.503LysMet: 0.503 ± 0.067
0.629LysAsn: 0.629 ± 0.058
1.138LysPro: 1.138 ± 0.077
0.772LysGln: 0.772 ± 0.069
1.647LysArg: 1.647 ± 0.107
1.161LysSer: 1.161 ± 0.085
1.419LysThr: 1.419 ± 0.093
1.808LysVal: 1.808 ± 0.12
0.349LysTrp: 0.349 ± 0.052
0.452LysTyr: 0.452 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
12.574LeuAla: 12.574 ± 0.313
0.938LeuCys: 0.938 ± 0.071
6.43LeuAsp: 6.43 ± 0.204
5.326LeuGlu: 5.326 ± 0.166
2.626LeuPhe: 2.626 ± 0.132
8.592LeuGly: 8.592 ± 0.249
2.351LeuHis: 2.351 ± 0.13
4.113LeuIle: 4.113 ± 0.155
1.825LeuLys: 1.825 ± 0.089
8.701LeuLeu: 8.701 ± 0.3
1.842LeuMet: 1.842 ± 0.124
2.3LeuAsn: 2.3 ± 0.111
5.085LeuPro: 5.085 ± 0.165
2.403LeuGln: 2.403 ± 0.105
7.259LeuArg: 7.259 ± 0.231
5.886LeuSer: 5.886 ± 0.196
6.91LeuThr: 6.91 ± 0.229
7.997LeuVal: 7.997 ± 0.208
1.19LeuTrp: 1.19 ± 0.086
1.527LeuTyr: 1.527 ± 0.1
0.0LeuXaa: 0.0 ± 0.0
Met
2.248MetAla: 2.248 ± 0.124
0.206MetCys: 0.206 ± 0.036
1.007MetAsp: 1.007 ± 0.08
0.784MetGlu: 0.784 ± 0.077
0.606MetPhe: 0.606 ± 0.065
1.607MetGly: 1.607 ± 0.103
0.429MetHis: 0.429 ± 0.044
0.995MetIle: 0.995 ± 0.08
0.641MetLys: 0.641 ± 0.058
1.934MetLeu: 1.934 ± 0.104
0.492MetMet: 0.492 ± 0.056
0.612MetAsn: 0.612 ± 0.065
1.161MetPro: 1.161 ± 0.087
0.629MetGln: 0.629 ± 0.065
1.567MetArg: 1.567 ± 0.093
1.831MetSer: 1.831 ± 0.11
2.099MetThr: 2.099 ± 0.12
1.596MetVal: 1.596 ± 0.101
0.275MetTrp: 0.275 ± 0.04
0.303MetTyr: 0.303 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
2.746AsnAla: 2.746 ± 0.144
0.229AsnCys: 0.229 ± 0.036
1.379AsnAsp: 1.379 ± 0.095
1.201AsnGlu: 1.201 ± 0.081
0.841AsnPhe: 0.841 ± 0.075
2.248AsnGly: 2.248 ± 0.131
0.549AsnHis: 0.549 ± 0.056
1.133AsnIle: 1.133 ± 0.064
0.486AsnLys: 0.486 ± 0.063
2.328AsnLeu: 2.328 ± 0.123
0.475AsnMet: 0.475 ± 0.053
0.686AsnAsn: 0.686 ± 0.069
1.785AsnPro: 1.785 ± 0.093
0.784AsnGln: 0.784 ± 0.07
1.979AsnArg: 1.979 ± 0.107
1.379AsnSer: 1.379 ± 0.094
1.556AsnThr: 1.556 ± 0.11
2.094AsnVal: 2.094 ± 0.109
0.4AsnTrp: 0.4 ± 0.047
0.612AsnTyr: 0.612 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
5.806ProAla: 5.806 ± 0.202
0.446ProCys: 0.446 ± 0.051
3.787ProAsp: 3.787 ± 0.175
3.404ProGlu: 3.404 ± 0.157
1.522ProPhe: 1.522 ± 0.095
4.897ProGly: 4.897 ± 0.198
1.47ProHis: 1.47 ± 0.093
2.357ProIle: 2.357 ± 0.119
1.098ProLys: 1.098 ± 0.075
4.582ProLeu: 4.582 ± 0.146
1.144ProMet: 1.144 ± 0.077
1.527ProAsn: 1.527 ± 0.094
2.677ProPro: 2.677 ± 0.152
1.59ProGln: 1.59 ± 0.108
3.501ProArg: 3.501 ± 0.144
3.261ProSer: 3.261 ± 0.13
3.472ProThr: 3.472 ± 0.152
4.588ProVal: 4.588 ± 0.209
0.858ProTrp: 0.858 ± 0.078
1.173ProTyr: 1.173 ± 0.086
0.0ProXaa: 0.0 ± 0.0
Gln
3.346GlnAla: 3.346 ± 0.141
0.2GlnCys: 0.2 ± 0.033
1.178GlnAsp: 1.178 ± 0.094
1.167GlnGlu: 1.167 ± 0.078
1.156GlnPhe: 1.156 ± 0.08
2.208GlnGly: 2.208 ± 0.109
0.715GlnHis: 0.715 ± 0.07
1.779GlnIle: 1.779 ± 0.116
0.698GlnLys: 0.698 ± 0.066
3.427GlnLeu: 3.427 ± 0.178
0.692GlnMet: 0.692 ± 0.062
0.664GlnAsn: 0.664 ± 0.061
1.647GlnPro: 1.647 ± 0.105
1.436GlnGln: 1.436 ± 0.094
2.443GlnArg: 2.443 ± 0.138
1.848GlnSer: 1.848 ± 0.097
1.699GlnThr: 1.699 ± 0.101
2.448GlnVal: 2.448 ± 0.118
0.561GlnTrp: 0.561 ± 0.059
0.652GlnTyr: 0.652 ± 0.06
0.0GlnXaa: 0.0 ± 0.0
Arg
8.232ArgAla: 8.232 ± 0.232
0.784ArgCys: 0.784 ± 0.086
4.285ArgAsp: 4.285 ± 0.182
4.433ArgGlu: 4.433 ± 0.195
2.58ArgPhe: 2.58 ± 0.13
5.303ArgGly: 5.303 ± 0.172
2.054ArgHis: 2.054 ± 0.112
4.05ArgIle: 4.05 ± 0.14
1.768ArgLys: 1.768 ± 0.092
7.179ArgLeu: 7.179 ± 0.222
1.819ArgMet: 1.819 ± 0.087
1.842ArgAsn: 1.842 ± 0.111
3.941ArgPro: 3.941 ± 0.153
2.425ArgGln: 2.425 ± 0.13
7.139ArgArg: 7.139 ± 0.284
4.473ArgSer: 4.473 ± 0.178
4.639ArgThr: 4.639 ± 0.189
5.034ArgVal: 5.034 ± 0.183
1.27ArgTrp: 1.27 ± 0.088
1.876ArgTyr: 1.876 ± 0.121
0.0ArgXaa: 0.0 ± 0.0
Ser
7.328SerAla: 7.328 ± 0.19
0.486SerCys: 0.486 ± 0.064
3.718SerAsp: 3.718 ± 0.132
2.814SerGlu: 2.814 ± 0.146
1.728SerPhe: 1.728 ± 0.104
5.909SerGly: 5.909 ± 0.207
1.333SerHis: 1.333 ± 0.091
2.998SerIle: 2.998 ± 0.138
1.373SerLys: 1.373 ± 0.091
5.148SerLeu: 5.148 ± 0.19
1.527SerMet: 1.527 ± 0.097
1.327SerAsn: 1.327 ± 0.091
3.501SerPro: 3.501 ± 0.15
1.522SerGln: 1.522 ± 0.104
4.285SerArg: 4.285 ± 0.17
3.695SerSer: 3.695 ± 0.173
4.102SerThr: 4.102 ± 0.171
4.759SerVal: 4.759 ± 0.164
1.03SerTrp: 1.03 ± 0.075
1.459SerTyr: 1.459 ± 0.096
0.0SerXaa: 0.0 ± 0.0
Thr
7.911ThrAla: 7.911 ± 0.263
0.566ThrCys: 0.566 ± 0.063
3.999ThrAsp: 3.999 ± 0.154
3.203ThrGlu: 3.203 ± 0.139
2.048ThrPhe: 2.048 ± 0.126
5.949ThrGly: 5.949 ± 0.205
1.384ThrHis: 1.384 ± 0.098
2.998ThrIle: 2.998 ± 0.135
1.384ThrLys: 1.384 ± 0.108
6.069ThrLeu: 6.069 ± 0.179
1.327ThrMet: 1.327 ± 0.085
1.487ThrAsn: 1.487 ± 0.102
3.781ThrPro: 3.781 ± 0.157
1.756ThrGln: 1.756 ± 0.111
4.285ThrArg: 4.285 ± 0.17
3.987ThrSer: 3.987 ± 0.151
4.359ThrThr: 4.359 ± 0.198
5.6ThrVal: 5.6 ± 0.155
0.915ThrTrp: 0.915 ± 0.081
1.419ThrTyr: 1.419 ± 0.096
0.0ThrXaa: 0.0 ± 0.0
Val
10.011ValAla: 10.011 ± 0.235
0.704ValCys: 0.704 ± 0.074
5.606ValAsp: 5.606 ± 0.178
4.674ValGlu: 4.674 ± 0.166
2.448ValPhe: 2.448 ± 0.135
6.996ValGly: 6.996 ± 0.238
1.859ValHis: 1.859 ± 0.103
3.735ValIle: 3.735 ± 0.145
1.67ValLys: 1.67 ± 0.087
8.083ValLeu: 8.083 ± 0.174
1.413ValMet: 1.413 ± 0.088
2.34ValAsn: 2.34 ± 0.122
4.227ValPro: 4.227 ± 0.168
1.991ValGln: 1.991 ± 0.118
5.686ValArg: 5.686 ± 0.189
4.782ValSer: 4.782 ± 0.18
5.326ValThr: 5.326 ± 0.189
6.847ValVal: 6.847 ± 0.23
1.144ValTrp: 1.144 ± 0.086
1.533ValTyr: 1.533 ± 0.109
0.0ValXaa: 0.0 ± 0.0
Trp
1.533TrpAla: 1.533 ± 0.091
0.229TrpCys: 0.229 ± 0.04
0.818TrpAsp: 0.818 ± 0.074
0.686TrpGlu: 0.686 ± 0.062
0.549TrpPhe: 0.549 ± 0.058
1.23TrpGly: 1.23 ± 0.089
0.338TrpHis: 0.338 ± 0.045
0.789TrpIle: 0.789 ± 0.07
0.475TrpLys: 0.475 ± 0.052
1.676TrpLeu: 1.676 ± 0.1
0.338TrpMet: 0.338 ± 0.045
0.521TrpAsn: 0.521 ± 0.061
0.618TrpPro: 0.618 ± 0.068
0.601TrpGln: 0.601 ± 0.059
1.196TrpArg: 1.196 ± 0.089
1.064TrpSer: 1.064 ± 0.079
1.041TrpThr: 1.041 ± 0.089
1.064TrpVal: 1.064 ± 0.094
0.263TrpTrp: 0.263 ± 0.04
0.343TrpTyr: 0.343 ± 0.052
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.282TyrAla: 2.282 ± 0.114
0.212TyrCys: 0.212 ± 0.033
1.333TyrAsp: 1.333 ± 0.087
1.121TyrGlu: 1.121 ± 0.083
0.749TyrPhe: 0.749 ± 0.063
2.111TyrGly: 2.111 ± 0.11
0.583TyrHis: 0.583 ± 0.064
0.835TyrIle: 0.835 ± 0.074
0.309TyrLys: 0.309 ± 0.044
2.271TyrLeu: 2.271 ± 0.11
0.338TyrMet: 0.338 ± 0.043
0.463TyrAsn: 0.463 ± 0.044
1.201TyrPro: 1.201 ± 0.083
0.664TyrGln: 0.664 ± 0.067
1.842TyrArg: 1.842 ± 0.108
1.436TyrSer: 1.436 ± 0.104
1.098TyrThr: 1.098 ± 0.09
1.579TyrVal: 1.579 ± 0.109
0.309TyrTrp: 0.309 ± 0.045
0.561TyrTyr: 0.561 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 635 proteins (174812 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski