Candidatus Cytomitobacter primus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales; Holosporaceae; Candidatus Cytomitobacter

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 519 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0UIW0|A0A5C0UIW0_9PROT YrdC-like domain-containing protein OS=Candidatus Cytomitobacter primus OX=2066024 GN=FZC34_02785 PE=4 SV=1
MM1 pKa = 7.28NFISNMIIDD10 pKa = 3.83EE11 pKa = 4.32MFDD14 pKa = 3.61KK15 pKa = 10.78FWNEE19 pKa = 3.21GDD21 pKa = 4.13HH22 pKa = 7.17DD23 pKa = 4.36VSSPVRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84SAEE34 pKa = 3.47SDD36 pKa = 2.88IYY38 pKa = 11.3LRR40 pKa = 11.84FLGAQNSAISIADD53 pKa = 3.34KK54 pKa = 10.95EE55 pKa = 4.65IINKK59 pKa = 8.95LQIDD63 pKa = 4.05DD64 pKa = 5.84RR65 pKa = 11.84ILNDD69 pKa = 3.66DD70 pKa = 4.12NLRR73 pKa = 11.84DD74 pKa = 4.35LIAPFMSQTDD84 pKa = 3.71TVGCLNTHH92 pKa = 6.14ILDD95 pKa = 3.94YY96 pKa = 11.07FDD98 pKa = 6.86DD99 pKa = 4.67IDD101 pKa = 4.03TADD104 pKa = 3.45STVYY108 pKa = 11.31VMLTFMQCFGFKK120 pKa = 10.25TIDD123 pKa = 3.85CFDD126 pKa = 4.14HH127 pKa = 6.58EE128 pKa = 4.9LNIDD132 pKa = 3.3AMIPNRR138 pKa = 11.84EE139 pKa = 3.88FYY141 pKa = 10.78QFNQKK146 pKa = 8.01FTFKK150 pKa = 10.73KK151 pKa = 10.91LEE153 pKa = 4.02ITCNTEE159 pKa = 4.22KK160 pKa = 10.65KK161 pKa = 10.05DD162 pKa = 3.73SKK164 pKa = 11.23PNIEE168 pKa = 4.6DD169 pKa = 4.71DD170 pKa = 3.85INIKK174 pKa = 10.73DD175 pKa = 4.11EE176 pKa = 3.97IVEE179 pKa = 3.97YY180 pKa = 10.75LINTASAQEE189 pKa = 3.81LSFYY193 pKa = 10.84AGKK196 pKa = 10.41DD197 pKa = 3.24CDD199 pKa = 3.57KK200 pKa = 10.27VARR203 pKa = 11.84MVNFVYY209 pKa = 10.48RR210 pKa = 11.84KK211 pKa = 8.71NPEE214 pKa = 3.32MFANLKK220 pKa = 10.26SVGIGVPVYY229 pKa = 11.15GDD231 pKa = 3.66GDD233 pKa = 3.41QDD235 pKa = 3.95LYY237 pKa = 11.35EE238 pKa = 4.14VAVFKK243 pKa = 11.39NDD245 pKa = 3.26VLDD248 pKa = 4.49VLDD251 pKa = 5.23EE252 pKa = 4.66DD253 pKa = 5.34LPCNMHH259 pKa = 5.97MVWMIQGGSDD269 pKa = 4.68SDD271 pKa = 4.66DD272 pKa = 3.21NWVADD277 pKa = 3.79PSDD280 pKa = 4.13YY281 pKa = 10.95IEE283 pKa = 4.74NGFDD287 pKa = 2.89HH288 pKa = 7.39FYY290 pKa = 11.5NEE292 pKa = 5.15FII294 pKa = 4.9

Molecular weight: 33.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0UF08|A0A5C0UF08_9PROT Uncharacterized protein OS=Candidatus Cytomitobacter primus OX=2066024 GN=FZC34_01810 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.19RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.67GFRR19 pKa = 11.84KK20 pKa = 9.96RR21 pKa = 11.84MSTQDD26 pKa = 2.87GRR28 pKa = 11.84NVMSARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.52GRR39 pKa = 11.84SKK41 pKa = 10.58ISAA44 pKa = 3.55

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

519

0

519

174856

41

2712

336.9

38.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.416 ± 0.091

1.35 ± 0.035

5.558 ± 0.092

6.109 ± 0.127

4.508 ± 0.088

5.204 ± 0.1

2.34 ± 0.044

9.669 ± 0.112

8.878 ± 0.102

9.002 ± 0.158

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.046

7.239 ± 0.118

2.78 ± 0.057

3.601 ± 0.071

3.324 ± 0.076

7.871 ± 0.088

4.218 ± 0.059

5.322 ± 0.088

0.876 ± 0.029

3.96 ± 0.07
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski