Nocardiopsis gilva YIM 90087

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Nocardiopsis; Nocardiopsis gilva

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5074 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223S2Q7|A0A223S2Q7_9ACTN DUF1996 domain-containing protein OS=Nocardiopsis gilva YIM 90087 OX=1235441 GN=CDO52_06055 PE=4 SV=1
MM1 pKa = 7.63DD2 pKa = 4.63RR3 pKa = 11.84KK4 pKa = 9.48RR5 pKa = 11.84TVRR8 pKa = 11.84AGNKK12 pKa = 7.66TLISVGMAAILLTAAGCTGDD32 pKa = 3.95GDD34 pKa = 4.01SGYY37 pKa = 10.92GGYY40 pKa = 10.73GGADD44 pKa = 3.51KK45 pKa = 11.1DD46 pKa = 4.16SATSDD51 pKa = 3.16EE52 pKa = 4.48GQNGAAATLQVAADD66 pKa = 3.75TSVGEE71 pKa = 4.41VVTDD75 pKa = 3.58ADD77 pKa = 4.72GYY79 pKa = 7.4TLYY82 pKa = 10.5MFTQDD87 pKa = 2.9STEE90 pKa = 4.3PPTSNCDD97 pKa = 3.27ADD99 pKa = 5.29CAKK102 pKa = 10.06QWPPALTEE110 pKa = 4.27GEE112 pKa = 4.16PTAEE116 pKa = 4.12GVEE119 pKa = 4.28SEE121 pKa = 4.9LIGTIEE127 pKa = 4.43RR128 pKa = 11.84EE129 pKa = 4.16DD130 pKa = 3.98GGTQLTLAGWPIYY143 pKa = 10.09RR144 pKa = 11.84YY145 pKa = 10.42AGDD148 pKa = 3.71VSPGDD153 pKa = 3.58VNGQGMGGTWYY164 pKa = 10.66AVTPEE169 pKa = 4.12GKK171 pKa = 9.5QAEE174 pKa = 4.69GTGAVGDD181 pKa = 4.43SEE183 pKa = 5.95DD184 pKa = 3.88GASPGTQGTQGTDD197 pKa = 2.76EE198 pKa = 4.88GGGSGGYY205 pKa = 9.61

Molecular weight: 20.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223S134|A0A223S134_9ACTN SHOCT domain-containing protein OS=Nocardiopsis gilva YIM 90087 OX=1235441 GN=CDO52_02640 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIAARR35 pKa = 11.84RR36 pKa = 11.84NKK38 pKa = 9.29GRR40 pKa = 11.84ARR42 pKa = 11.84LTVSQQ47 pKa = 4.05

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5074

0

5074

1623985

31

5684

320.1

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.911 ± 0.056

0.813 ± 0.011

6.469 ± 0.031

6.181 ± 0.033

2.811 ± 0.019

9.174 ± 0.033

2.284 ± 0.019

3.79 ± 0.024

1.986 ± 0.031

9.998 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.973 ± 0.015

1.831 ± 0.017

5.912 ± 0.03

2.654 ± 0.018

8.119 ± 0.041

5.289 ± 0.02

5.941 ± 0.022

8.325 ± 0.036

1.484 ± 0.014

2.053 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski