Marchantia polymorpha (Liverwort) (Marchantia aquatica)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Marchantiophyta; Marchantiopsida; Marchantiidae; Marchantiales; Marchantiaceae; Marchantia

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R6WP00|A0A2R6WP00_MARPO Fatty acid 2-hydroxylase OS=Marchantia polymorpha OX=3197 GN=MARPO_0070s0052 PE=3 SV=1
MM1 pKa = 7.33SPSHH5 pKa = 6.41VSTTIFVSFAVVVGCVCIFIVIRR28 pKa = 11.84GIFPLPDD35 pKa = 3.21YY36 pKa = 10.83SSQSEE41 pKa = 4.31SGGAIPEE48 pKa = 4.21PSEE51 pKa = 4.75HH52 pKa = 5.68NTGDD56 pKa = 3.72LEE58 pKa = 4.23EE59 pKa = 4.66EE60 pKa = 4.61SVGGADD66 pKa = 4.96DD67 pKa = 4.44GAHH70 pKa = 6.42GEE72 pKa = 4.25AGADD76 pKa = 3.01SGGTLRR82 pKa = 11.84GDD84 pKa = 3.37GGGVVGDD91 pKa = 3.88GSGGGGAAAVNSS103 pKa = 4.09

Molecular weight: 9.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R6VX42|A0A2R6VX42_MARPO Uncharacterized protein OS=Marchantia polymorpha OX=3197 GN=MARPO_YA0033 PE=4 SV=1
MM1 pKa = 7.8ASPGDD6 pKa = 4.22LMSGDD11 pKa = 3.97PSGSTKK17 pKa = 10.6ARR19 pKa = 11.84GKK21 pKa = 10.24GLQHH25 pKa = 5.77PTYY28 pKa = 11.29ANLIHH33 pKa = 7.25DD34 pKa = 6.04AISSLKK40 pKa = 9.84QRR42 pKa = 11.84GGSSAIAIRR51 pKa = 11.84KK52 pKa = 9.59SIEE55 pKa = 4.03QKK57 pKa = 10.55VGPTLGPNYY66 pKa = 10.05KK67 pKa = 10.16KK68 pKa = 10.43IVGSTLSKK76 pKa = 10.21LAKK79 pKa = 8.64TGKK82 pKa = 9.92LIKK85 pKa = 10.5SGNTFKK91 pKa = 11.08LSPEE95 pKa = 4.01FKK97 pKa = 9.69IEE99 pKa = 3.51HH100 pKa = 5.74LRR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84HH109 pKa = 4.87HH110 pKa = 6.87KK111 pKa = 9.83KK112 pKa = 9.99GKK114 pKa = 8.67KK115 pKa = 8.22KK116 pKa = 9.73GRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84KK123 pKa = 8.11RR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84GRR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84AGTTTLTASGGVAVARR146 pKa = 11.84RR147 pKa = 11.84RR148 pKa = 11.84RR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84RR153 pKa = 11.84RR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84SRR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84RR164 pKa = 11.84GRR166 pKa = 11.84KK167 pKa = 7.03HH168 pKa = 3.92TTRR171 pKa = 11.84SSSAPGITGLRR182 pKa = 11.84RR183 pKa = 11.84RR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84RR187 pKa = 11.84KK188 pKa = 7.98SRR190 pKa = 11.84RR191 pKa = 11.84RR192 pKa = 11.84RR193 pKa = 11.84RR194 pKa = 11.84RR195 pKa = 11.84RR196 pKa = 11.84RR197 pKa = 11.84RR198 pKa = 11.84RR199 pKa = 11.84SKK201 pKa = 10.31GSRR204 pKa = 11.84SPRR207 pKa = 11.84RR208 pKa = 11.84RR209 pKa = 11.84HH210 pKa = 5.17SSRR213 pKa = 11.84SSGVSQRR220 pKa = 11.84RR221 pKa = 11.84VRR223 pKa = 11.84HH224 pKa = 5.61ASHH227 pKa = 5.46VHH229 pKa = 3.46IHH231 pKa = 6.43GGHH234 pKa = 5.74SRR236 pKa = 11.84GGSPDD241 pKa = 3.29SPSGHH246 pKa = 7.21RR247 pKa = 11.84SPQLL251 pKa = 3.26

Molecular weight: 28.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19120

2736

21856

9495625

29

9934

434.5

48.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.035 ± 0.018

1.733 ± 0.008

5.316 ± 0.012

6.634 ± 0.022

3.65 ± 0.011

7.054 ± 0.016

2.335 ± 0.007

4.337 ± 0.012

5.251 ± 0.018

9.449 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.309 ± 0.006

3.595 ± 0.012

5.333 ± 0.019

4.052 ± 0.015

6.314 ± 0.015

8.871 ± 0.025

5.184 ± 0.012

6.804 ± 0.013

1.303 ± 0.006

2.439 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski