Tomato leaf curl Karnataka virus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5N3D0|V5N3D0_9GEMI Transcriptional activator protein OS=Tomato leaf curl Karnataka virus 2 OX=2560810 PE=3 SV=1
MM1 pKa = 7.68APPSRR6 pKa = 11.84FRR8 pKa = 11.84INAKK12 pKa = 10.4NYY14 pKa = 8.23FLTYY18 pKa = 9.88LKK20 pKa = 10.78CSLTKK25 pKa = 10.75EE26 pKa = 4.13EE27 pKa = 5.36ALSQLLNLQTPTSKK41 pKa = 10.73KK42 pKa = 9.67FIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.49LHH51 pKa = 6.58EE52 pKa = 6.12DD53 pKa = 3.79GTPHH57 pKa = 6.72LHH59 pKa = 6.66VLIQFEE65 pKa = 5.54GKK67 pKa = 9.21FQCKK71 pKa = 8.51NNRR74 pKa = 11.84FFDD77 pKa = 3.77LTSPTRR83 pKa = 11.84SAHH86 pKa = 4.94FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.05VKK101 pKa = 11.21SYY103 pKa = 9.59MEE105 pKa = 4.47KK106 pKa = 10.83DD107 pKa = 3.45GDD109 pKa = 4.06VLDD112 pKa = 4.63HH113 pKa = 7.26GVFQVDD119 pKa = 3.16GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.21AQALNILKK151 pKa = 10.06EE152 pKa = 3.83KK153 pKa = 10.53APRR156 pKa = 11.84DD157 pKa = 3.61FLFQFHH163 pKa = 6.77NLNSNLDD170 pKa = 3.69RR171 pKa = 11.84FFTPPMEE178 pKa = 4.51VYY180 pKa = 10.7VSPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.98EE195 pKa = 4.13LEE197 pKa = 3.58EE198 pKa = 3.9WAAEE202 pKa = 4.06NVVRR206 pKa = 11.84SAARR210 pKa = 11.84PLRR213 pKa = 11.84PVSIVIEE220 pKa = 4.4GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.39LSPKK249 pKa = 10.12VYY251 pKa = 11.11NNDD254 pKa = 2.08AWYY257 pKa = 10.88NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.37KK312 pKa = 10.66EE313 pKa = 3.86FLDD316 pKa = 3.54EE317 pKa = 5.43DD318 pKa = 4.07KK319 pKa = 11.59NSALKK324 pKa = 10.52NWALKK329 pKa = 10.24NAIFVTLEE337 pKa = 3.93GPLYY341 pKa = 10.71SGSHH345 pKa = 4.84QSAAQASQEE354 pKa = 3.81GDD356 pKa = 3.31QTSTCC361 pKa = 4.0

Molecular weight: 40.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5N3Z7|V5N3Z7_9GEMI Protein V2 OS=Tomato leaf curl Karnataka virus 2 OX=2560810 PE=3 SV=1
MM1 pKa = 7.93AKK3 pKa = 9.91RR4 pKa = 11.84PADD7 pKa = 3.74IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.14SPYY27 pKa = 9.74GARR30 pKa = 11.84AVVPIARR37 pKa = 11.84VTKK40 pKa = 10.46AKK42 pKa = 10.49AWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.8PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.06SRR80 pKa = 11.84HH81 pKa = 5.57DD82 pKa = 3.47VSHH85 pKa = 6.92IGKK88 pKa = 9.26VMCVSDD94 pKa = 3.59VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.89RR143 pKa = 11.84RR144 pKa = 11.84PTGSPQDD151 pKa = 3.6FGEE154 pKa = 4.31VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.6ATVTGGTYY191 pKa = 10.43ACRR194 pKa = 11.84EE195 pKa = 3.76QALVRR200 pKa = 11.84KK201 pKa = 8.27FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.99ATLKK245 pKa = 8.67VRR247 pKa = 11.84SYY249 pKa = 10.98FYY251 pKa = 11.25DD252 pKa = 3.13SVTNN256 pKa = 3.98

Molecular weight: 29.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1100

97

361

183.3

20.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.818 ± 0.669

2.273 ± 0.319

4.818 ± 0.453

4.545 ± 0.513

4.455 ± 0.684

4.909 ± 0.449

4.182 ± 0.637

4.182 ± 0.956

6.273 ± 0.317

7.273 ± 0.967

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.63

5.455 ± 0.713

5.636 ± 0.373

4.818 ± 0.684

6.636 ± 1.101

8.727 ± 1.345

5.909 ± 0.776

6.545 ± 1.333

1.455 ± 0.139

3.455 ± 0.588
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski