Amino acid dipepetide frequency for Euzebya sp. DY32-46

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.287AlaAla: 19.287 ± 0.167
0.998AlaCys: 0.998 ± 0.024
10.494AlaAsp: 10.494 ± 0.093
8.123AlaGlu: 8.123 ± 0.079
3.58AlaPhe: 3.58 ± 0.043
12.289AlaGly: 12.289 ± 0.093
2.649AlaHis: 2.649 ± 0.045
5.048AlaIle: 5.048 ± 0.05
1.821AlaLys: 1.821 ± 0.044
12.494AlaLeu: 12.494 ± 0.103
3.056AlaMet: 3.056 ± 0.044
2.34AlaAsn: 2.34 ± 0.036
6.226AlaPro: 6.226 ± 0.073
3.269AlaGln: 3.269 ± 0.051
8.516AlaArg: 8.516 ± 0.085
6.251AlaSer: 6.251 ± 0.065
8.452AlaThr: 8.452 ± 0.079
12.129AlaVal: 12.129 ± 0.098
1.854AlaTrp: 1.854 ± 0.036
2.047AlaTyr: 2.047 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
0.81CysAla: 0.81 ± 0.02
0.072CysCys: 0.072 ± 0.006
0.559CysAsp: 0.559 ± 0.019
0.378CysGlu: 0.378 ± 0.013
0.162CysPhe: 0.162 ± 0.01
0.805CysGly: 0.805 ± 0.024
0.194CysHis: 0.194 ± 0.011
0.216CysIle: 0.216 ± 0.012
0.091CysLys: 0.091 ± 0.008
0.51CysLeu: 0.51 ± 0.017
0.126CysMet: 0.126 ± 0.008
0.154CysAsn: 0.154 ± 0.01
0.472CysPro: 0.472 ± 0.019
0.19CysGln: 0.19 ± 0.011
0.557CysArg: 0.557 ± 0.02
0.389CysSer: 0.389 ± 0.018
0.504CysThr: 0.504 ± 0.019
0.515CysVal: 0.515 ± 0.017
0.102CysTrp: 0.102 ± 0.008
0.137CysTyr: 0.137 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
9.761AspAla: 9.761 ± 0.085
0.425AspCys: 0.425 ± 0.014
6.036AspAsp: 6.036 ± 0.075
4.384AspGlu: 4.384 ± 0.058
1.631AspPhe: 1.631 ± 0.03
8.695AspGly: 8.695 ± 0.094
1.91AspHis: 1.91 ± 0.031
2.509AspIle: 2.509 ± 0.041
0.785AspLys: 0.785 ± 0.021
7.605AspLeu: 7.605 ± 0.066
1.118AspMet: 1.118 ± 0.028
1.256AspAsn: 1.256 ± 0.028
5.76AspPro: 5.76 ± 0.069
1.848AspGln: 1.848 ± 0.032
6.134AspArg: 6.134 ± 0.065
2.576AspSer: 2.576 ± 0.042
4.166AspThr: 4.166 ± 0.053
6.847AspVal: 6.847 ± 0.06
1.004AspTrp: 1.004 ± 0.023
1.118AspTyr: 1.118 ± 0.027
0.0AspXaa: 0.0 ± 0.0
Glu
7.49GluAla: 7.49 ± 0.081
0.294GluCys: 0.294 ± 0.012
3.743GluAsp: 3.743 ± 0.045
3.681GluGlu: 3.681 ± 0.063
1.327GluPhe: 1.327 ± 0.029
4.797GluGly: 4.797 ± 0.056
1.331GluHis: 1.331 ± 0.032
2.271GluIle: 2.271 ± 0.037
0.754GluLys: 0.754 ± 0.024
5.885GluLeu: 5.885 ± 0.065
1.074GluMet: 1.074 ± 0.027
0.766GluAsn: 0.766 ± 0.019
3.229GluPro: 3.229 ± 0.056
2.217GluGln: 2.217 ± 0.039
4.696GluArg: 4.696 ± 0.067
2.373GluSer: 2.373 ± 0.039
2.987GluThr: 2.987 ± 0.043
5.659GluVal: 5.659 ± 0.061
0.862GluTrp: 0.862 ± 0.021
0.805GluTyr: 0.805 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.517PheAla: 3.517 ± 0.039
0.213PheCys: 0.213 ± 0.011
2.344PheAsp: 2.344 ± 0.038
1.49PheGlu: 1.49 ± 0.027
0.737PhePhe: 0.737 ± 0.022
2.927PheGly: 2.927 ± 0.049
0.597PheHis: 0.597 ± 0.018
0.73PheIle: 0.73 ± 0.02
0.331PheLys: 0.331 ± 0.013
2.26PheLeu: 2.26 ± 0.038
0.421PheMet: 0.421 ± 0.014
0.563PheAsn: 0.563 ± 0.018
1.35PhePro: 1.35 ± 0.027
0.565PheGln: 0.565 ± 0.018
1.535PheArg: 1.535 ± 0.03
1.342PheSer: 1.342 ± 0.032
1.869PheThr: 1.869 ± 0.03
2.614PheVal: 2.614 ± 0.04
0.344PheTrp: 0.344 ± 0.013
0.441PheTyr: 0.441 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
9.762GlyAla: 9.762 ± 0.089
0.795GlyCys: 0.795 ± 0.024
6.19GlyAsp: 6.19 ± 0.067
4.991GlyGlu: 4.991 ± 0.059
2.81GlyPhe: 2.81 ± 0.036
8.738GlyGly: 8.738 ± 0.097
2.282GlyHis: 2.282 ± 0.042
3.748GlyIle: 3.748 ± 0.046
1.511GlyLys: 1.511 ± 0.035
9.378GlyLeu: 9.378 ± 0.082
2.313GlyMet: 2.313 ± 0.041
1.901GlyAsn: 1.901 ± 0.04
5.399GlyPro: 5.399 ± 0.061
2.939GlyGln: 2.939 ± 0.042
7.218GlyArg: 7.218 ± 0.073
5.083GlySer: 5.083 ± 0.058
6.636GlyThr: 6.636 ± 0.088
8.028GlyVal: 8.028 ± 0.073
1.881GlyTrp: 1.881 ± 0.037
1.929GlyTyr: 1.929 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.644HisAla: 2.644 ± 0.039
0.168HisCys: 0.168 ± 0.01
1.929HisAsp: 1.929 ± 0.031
1.285HisGlu: 1.285 ± 0.029
0.5HisPhe: 0.5 ± 0.017
2.295HisGly: 2.295 ± 0.042
0.767HisHis: 0.767 ± 0.022
0.665HisIle: 0.665 ± 0.019
0.236HisLys: 0.236 ± 0.012
2.42HisLeu: 2.42 ± 0.038
0.329HisMet: 0.329 ± 0.012
0.388HisAsn: 0.388 ± 0.013
1.744HisPro: 1.744 ± 0.032
0.606HisGln: 0.606 ± 0.02
1.955HisArg: 1.955 ± 0.037
0.837HisSer: 0.837 ± 0.024
1.273HisThr: 1.273 ± 0.03
1.961HisVal: 1.961 ± 0.031
0.257HisTrp: 0.257 ± 0.012
0.373HisTyr: 0.373 ± 0.013
0.0HisXaa: 0.0 ± 0.0
Ile
5.384IleAla: 5.384 ± 0.062
0.268IleCys: 0.268 ± 0.013
3.526IleAsp: 3.526 ± 0.048
2.428IleGlu: 2.428 ± 0.035
0.716IlePhe: 0.716 ± 0.022
4.176IleGly: 4.176 ± 0.048
0.784IleHis: 0.784 ± 0.02
1.204IleIle: 1.204 ± 0.03
0.516IleLys: 0.516 ± 0.017
2.639IleLeu: 2.639 ± 0.044
0.48IleMet: 0.48 ± 0.015
0.81IleAsn: 0.81 ± 0.022
1.938IlePro: 1.938 ± 0.034
0.821IleGln: 0.821 ± 0.024
2.469IleArg: 2.469 ± 0.039
1.757IleSer: 1.757 ± 0.028
2.582IleThr: 2.582 ± 0.042
3.595IleVal: 3.595 ± 0.045
0.386IleTrp: 0.386 ± 0.014
0.515IleTyr: 0.515 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
1.926LysAla: 1.926 ± 0.042
0.066LysCys: 0.066 ± 0.006
0.852LysAsp: 0.852 ± 0.025
0.678LysGlu: 0.678 ± 0.023
0.284LysPhe: 0.284 ± 0.014
1.245LysGly: 1.245 ± 0.032
0.272LysHis: 0.272 ± 0.011
0.474LysIle: 0.474 ± 0.018
0.422LysLys: 0.422 ± 0.021
1.281LysLeu: 1.281 ± 0.033
0.27LysMet: 0.27 ± 0.012
0.25LysAsn: 0.25 ± 0.012
0.817LysPro: 0.817 ± 0.026
0.456LysGln: 0.456 ± 0.016
1.094LysArg: 1.094 ± 0.029
0.723LysSer: 0.723 ± 0.025
0.82LysThr: 0.82 ± 0.022
1.39LysVal: 1.39 ± 0.032
0.182LysTrp: 0.182 ± 0.011
0.226LysTyr: 0.226 ± 0.012
0.0LysXaa: 0.0 ± 0.0
Leu
14.11LeuAla: 14.11 ± 0.114
0.599LeuCys: 0.599 ± 0.018
7.7LeuAsp: 7.7 ± 0.069
5.292LeuGlu: 5.292 ± 0.067
2.272LeuPhe: 2.272 ± 0.039
8.908LeuGly: 8.908 ± 0.081
2.143LeuHis: 2.143 ± 0.037
3.041LeuIle: 3.041 ± 0.05
1.253LeuLys: 1.253 ± 0.036
10.757LeuLeu: 10.757 ± 0.104
1.507LeuMet: 1.507 ± 0.029
1.539LeuAsn: 1.539 ± 0.029
5.793LeuPro: 5.793 ± 0.057
2.677LeuGln: 2.677 ± 0.041
7.129LeuArg: 7.129 ± 0.076
5.263LeuSer: 5.263 ± 0.055
6.064LeuThr: 6.064 ± 0.055
9.565LeuVal: 9.565 ± 0.068
1.083LeuTrp: 1.083 ± 0.027
1.422LeuTyr: 1.422 ± 0.026
0.0LeuXaa: 0.0 ± 0.0
Met
2.806MetAla: 2.806 ± 0.046
0.113MetCys: 0.113 ± 0.008
1.181MetAsp: 1.181 ± 0.029
0.866MetGlu: 0.866 ± 0.024
0.493MetPhe: 0.493 ± 0.016
1.607MetGly: 1.607 ± 0.035
0.413MetHis: 0.413 ± 0.017
0.765MetIle: 0.765 ± 0.019
0.347MetLys: 0.347 ± 0.015
1.895MetLeu: 1.895 ± 0.033
0.37MetMet: 0.37 ± 0.016
0.384MetAsn: 0.384 ± 0.014
1.175MetPro: 1.175 ± 0.024
0.544MetGln: 0.544 ± 0.017
1.407MetArg: 1.407 ± 0.029
1.327MetSer: 1.327 ± 0.026
1.712MetThr: 1.712 ± 0.031
1.718MetVal: 1.718 ± 0.032
0.228MetTrp: 0.228 ± 0.011
0.274MetTyr: 0.274 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.249AsnAla: 2.249 ± 0.031
0.132AsnCys: 0.132 ± 0.008
1.371AsnAsp: 1.371 ± 0.033
0.872AsnGlu: 0.872 ± 0.018
0.46AsnPhe: 0.46 ± 0.016
2.08AsnGly: 2.08 ± 0.039
0.429AsnHis: 0.429 ± 0.014
0.663AsnIle: 0.663 ± 0.019
0.258AsnLys: 0.258 ± 0.013
1.722AsnLeu: 1.722 ± 0.03
0.283AsnMet: 0.283 ± 0.013
0.429AsnAsn: 0.429 ± 0.017
1.524AsnPro: 1.524 ± 0.031
0.486AsnGln: 0.486 ± 0.018
1.337AsnArg: 1.337 ± 0.03
0.738AsnSer: 0.738 ± 0.021
1.13AsnThr: 1.13 ± 0.028
1.521AsnVal: 1.521 ± 0.03
0.211AsnTrp: 0.211 ± 0.011
0.33AsnTyr: 0.33 ± 0.016
0.0AsnXaa: 0.0 ± 0.0
Pro
7.801ProAla: 7.801 ± 0.093
0.336ProCys: 0.336 ± 0.014
5.741ProAsp: 5.741 ± 0.067
3.882ProGlu: 3.882 ± 0.057
1.607ProPhe: 1.607 ± 0.029
5.434ProGly: 5.434 ± 0.061
1.205ProHis: 1.205 ± 0.027
2.044ProIle: 2.044 ± 0.032
0.799ProLys: 0.799 ± 0.023
5.081ProLeu: 5.081 ± 0.053
1.137ProMet: 1.137 ± 0.027
1.066ProAsn: 1.066 ± 0.026
3.785ProPro: 3.785 ± 0.082
1.438ProGln: 1.438 ± 0.031
3.262ProArg: 3.262 ± 0.052
3.595ProSer: 3.595 ± 0.048
4.703ProThr: 4.703 ± 0.067
5.308ProVal: 5.308 ± 0.059
0.849ProTrp: 0.849 ± 0.025
0.935ProTyr: 0.935 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.796GlnAla: 3.796 ± 0.048
0.146GlnCys: 0.146 ± 0.008
1.482GlnAsp: 1.482 ± 0.028
1.239GlnGlu: 1.239 ± 0.029
0.643GlnPhe: 0.643 ± 0.021
2.093GlnGly: 2.093 ± 0.037
0.648GlnHis: 0.648 ± 0.019
1.057GlnIle: 1.057 ± 0.025
0.386GlnLys: 0.386 ± 0.018
3.071GlnLeu: 3.071 ± 0.044
0.593GlnMet: 0.593 ± 0.018
0.386GlnAsn: 0.386 ± 0.017
2.077GlnPro: 2.077 ± 0.043
1.331GlnGln: 1.331 ± 0.032
2.609GlnArg: 2.609 ± 0.043
1.2GlnSer: 1.2 ± 0.031
1.518GlnThr: 1.518 ± 0.027
2.683GlnVal: 2.683 ± 0.041
0.373GlnTrp: 0.373 ± 0.016
0.406GlnTyr: 0.406 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
8.452ArgAla: 8.452 ± 0.094
0.53ArgCys: 0.53 ± 0.019
4.482ArgAsp: 4.482 ± 0.05
3.592ArgGlu: 3.592 ± 0.052
2.375ArgPhe: 2.375 ± 0.035
5.095ArgGly: 5.095 ± 0.055
1.827ArgHis: 1.827 ± 0.037
3.43ArgIle: 3.43 ± 0.046
1.056ArgLys: 1.056 ± 0.03
7.815ArgLeu: 7.815 ± 0.076
1.811ArgMet: 1.811 ± 0.034
1.39ArgAsn: 1.39 ± 0.026
4.266ArgPro: 4.266 ± 0.052
2.274ArgGln: 2.274 ± 0.034
6.762ArgArg: 6.762 ± 0.082
3.8ArgSer: 3.8 ± 0.047
4.818ArgThr: 4.818 ± 0.058
6.202ArgVal: 6.202 ± 0.066
1.481ArgTrp: 1.481 ± 0.029
1.517ArgTyr: 1.517 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.098SerAla: 6.098 ± 0.063
0.381SerCys: 0.381 ± 0.017
3.133SerAsp: 3.133 ± 0.048
2.205SerGlu: 2.205 ± 0.038
1.524SerPhe: 1.524 ± 0.03
5.349SerGly: 5.349 ± 0.066
1.04SerHis: 1.04 ± 0.023
1.878SerIle: 1.878 ± 0.033
0.664SerLys: 0.664 ± 0.021
4.625SerLeu: 4.625 ± 0.051
1.265SerMet: 1.265 ± 0.026
0.983SerAsn: 0.983 ± 0.024
3.126SerPro: 3.126 ± 0.041
1.269SerGln: 1.269 ± 0.026
3.66SerArg: 3.66 ± 0.045
3.052SerSer: 3.052 ± 0.045
3.711SerThr: 3.711 ± 0.058
4.107SerVal: 4.107 ± 0.05
0.894SerTrp: 0.894 ± 0.022
0.896SerTyr: 0.896 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
8.76ThrAla: 8.76 ± 0.083
0.481ThrCys: 0.481 ± 0.018
5.072ThrAsp: 5.072 ± 0.059
3.263ThrGlu: 3.263 ± 0.048
1.89ThrPhe: 1.89 ± 0.033
6.381ThrGly: 6.381 ± 0.072
1.358ThrHis: 1.358 ± 0.028
2.78ThrIle: 2.78 ± 0.044
0.876ThrLys: 0.876 ± 0.027
5.686ThrLeu: 5.686 ± 0.054
1.241ThrMet: 1.241 ± 0.026
1.299ThrAsn: 1.299 ± 0.03
4.41ThrPro: 4.41 ± 0.075
1.393ThrGln: 1.393 ± 0.024
3.937ThrArg: 3.937 ± 0.046
3.566ThrSer: 3.566 ± 0.048
4.884ThrThr: 4.884 ± 0.073
6.664ThrVal: 6.664 ± 0.069
0.973ThrTrp: 0.973 ± 0.025
1.253ThrTyr: 1.253 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
12.602ValAla: 12.602 ± 0.096
0.632ValCys: 0.632 ± 0.02
7.491ValAsp: 7.491 ± 0.068
5.959ValGlu: 5.959 ± 0.06
2.205ValPhe: 2.205 ± 0.036
7.921ValGly: 7.921 ± 0.072
2.094ValHis: 2.094 ± 0.033
3.474ValIle: 3.474 ± 0.049
1.181ValLys: 1.181 ± 0.027
9.682ValLeu: 9.682 ± 0.083
1.664ValMet: 1.664 ± 0.031
1.698ValAsn: 1.698 ± 0.033
5.246ValPro: 5.246 ± 0.055
2.375ValGln: 2.375 ± 0.038
6.133ValArg: 6.133 ± 0.056
4.288ValSer: 4.288 ± 0.055
6.123ValThr: 6.123 ± 0.075
10.203ValVal: 10.203 ± 0.095
1.096ValTrp: 1.096 ± 0.024
1.297ValTyr: 1.297 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.697TrpAla: 1.697 ± 0.033
0.146TrpCys: 0.146 ± 0.009
0.905TrpAsp: 0.905 ± 0.025
0.675TrpGlu: 0.675 ± 0.021
0.485TrpPhe: 0.485 ± 0.017
1.025TrpGly: 1.025 ± 0.024
0.346TrpHis: 0.346 ± 0.015
0.573TrpIle: 0.573 ± 0.018
0.212TrpLys: 0.212 ± 0.011
1.613TrpLeu: 1.613 ± 0.03
0.332TrpMet: 0.332 ± 0.013
0.311TrpAsn: 0.311 ± 0.014
0.783TrpPro: 0.783 ± 0.022
0.568TrpGln: 0.568 ± 0.018
1.093TrpArg: 1.093 ± 0.028
0.901TrpSer: 0.901 ± 0.029
1.174TrpThr: 1.174 ± 0.027
1.225TrpVal: 1.225 ± 0.028
0.303TrpTrp: 0.303 ± 0.013
0.264TrpTyr: 0.264 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.969TyrAla: 1.969 ± 0.037
0.148TyrCys: 0.148 ± 0.008
1.336TyrAsp: 1.336 ± 0.032
0.957TyrGlu: 0.957 ± 0.022
0.49TyrPhe: 0.49 ± 0.016
1.671TyrGly: 1.671 ± 0.028
0.344TyrHis: 0.344 ± 0.012
0.425TyrIle: 0.425 ± 0.016
0.229TyrLys: 0.229 ± 0.012
1.7TyrLeu: 1.7 ± 0.03
0.236TyrMet: 0.236 ± 0.012
0.311TyrAsn: 0.311 ± 0.014
0.889TyrPro: 0.889 ± 0.026
0.471TyrGln: 0.471 ± 0.016
1.473TyrArg: 1.473 ± 0.028
0.801TyrSer: 0.801 ± 0.022
1.043TyrThr: 1.043 ± 0.028
1.429TyrVal: 1.429 ± 0.03
0.263TyrTrp: 0.263 ± 0.013
0.35TyrTyr: 0.35 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5625 proteins (1909651 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski