Azospirillum sp. L-25-5w-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Azospirillum

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S0R6U4|A0A3S0R6U4_9PROT Dabb family protein OS=Azospirillum sp. L-25-5w-1 OX=2496639 GN=EJ903_19160 PE=4 SV=1
MM1 pKa = 7.62ASGISFLAGSTVKK14 pKa = 10.57SVNTYY19 pKa = 8.5TSSNQSYY26 pKa = 8.74PAIGSFNDD34 pKa = 3.38GGYY37 pKa = 8.4VVSWISYY44 pKa = 8.63LQDD47 pKa = 3.02GSGWGVYY54 pKa = 7.15GQRR57 pKa = 11.84FSTDD61 pKa = 4.16GIPVGGEE68 pKa = 3.26FRR70 pKa = 11.84INVSTTGDD78 pKa = 3.32QDD80 pKa = 3.63SPSIVTHH87 pKa = 6.48SDD89 pKa = 2.8GSFNVYY95 pKa = 7.51WASYY99 pKa = 7.98ATAGVPYY106 pKa = 8.13TTAANIYY113 pKa = 9.37GRR115 pKa = 11.84RR116 pKa = 11.84FDD118 pKa = 4.99ASGNPLGSEE127 pKa = 4.17FKK129 pKa = 10.98VNSATTSDD137 pKa = 3.56RR138 pKa = 11.84NRR140 pKa = 11.84VGTAKK145 pKa = 10.54LADD148 pKa = 3.97GGEE151 pKa = 4.39VVVWTLRR158 pKa = 11.84NAAWTTAEE166 pKa = 4.94LYY168 pKa = 10.5AQRR171 pKa = 11.84FDD173 pKa = 3.65SAGNSVGGEE182 pKa = 3.65FRR184 pKa = 11.84VNPTVTAASSGQPNSVAGLDD204 pKa = 3.56GGGFAVVWDD213 pKa = 4.22ASDD216 pKa = 3.97GNGSGVYY223 pKa = 7.74VQRR226 pKa = 11.84FDD228 pKa = 4.09ASGNRR233 pKa = 11.84VGMEE237 pKa = 3.66TRR239 pKa = 11.84VNSTVASDD247 pKa = 3.4QSMGNITALAGGGFVVSWTSNGQDD271 pKa = 3.07GGGTGVYY278 pKa = 9.81AQRR281 pKa = 11.84FDD283 pKa = 3.83AAGTPVGTEE292 pKa = 3.93FQVNTQTAANQYY304 pKa = 8.91GSSAIGSPDD313 pKa = 3.03GGFTILYY320 pKa = 6.96TAGDD324 pKa = 3.89NQDD327 pKa = 2.92GSGAGVYY334 pKa = 7.88GQRR337 pKa = 11.84YY338 pKa = 8.88DD339 pKa = 3.73SNGLRR344 pKa = 11.84VGGEE348 pKa = 3.63FRR350 pKa = 11.84VNDD353 pKa = 3.86STVGNQDD360 pKa = 2.86AQVIGVRR367 pKa = 11.84PDD369 pKa = 2.93GGFVVAWNSPDD380 pKa = 3.43ASQSGVLTRR389 pKa = 11.84IYY391 pKa = 10.88SNPTAPYY398 pKa = 9.89SVAGEE403 pKa = 3.9QRR405 pKa = 11.84VNTTGTGDD413 pKa = 3.4QTRR416 pKa = 11.84SSISVFADD424 pKa = 3.34GGYY427 pKa = 9.8VVTWVSAGQAGSGDD441 pKa = 3.3GVYY444 pKa = 10.46AQRR447 pKa = 11.84YY448 pKa = 8.61DD449 pKa = 3.65SGGKK453 pKa = 9.42PIGSEE458 pKa = 3.86FRR460 pKa = 11.84VNTTTTGDD468 pKa = 3.29QGDD471 pKa = 4.3ATVVAYY477 pKa = 10.37DD478 pKa = 4.03GGQFAIFWASHH489 pKa = 4.15TQAGQTSPVDD499 pKa = 3.25ASVRR503 pKa = 11.84GQRR506 pKa = 11.84YY507 pKa = 8.4AADD510 pKa = 3.77GTPVGSEE517 pKa = 3.94FQVNGASTSDD527 pKa = 3.62RR528 pKa = 11.84NPPGAVRR535 pKa = 11.84LADD538 pKa = 3.67GSTVVVWLQHH548 pKa = 5.94DD549 pKa = 4.31SSWAHH554 pKa = 4.28TWVYY558 pKa = 8.8GQRR561 pKa = 11.84YY562 pKa = 8.46DD563 pKa = 3.43AAGNAVGGEE572 pKa = 3.93FLVNSVNSANSGYY585 pKa = 9.95VASGYY590 pKa = 10.29QPASVTALEE599 pKa = 4.3NGGFAVVWNAADD611 pKa = 3.86GDD613 pKa = 4.14GTGAYY618 pKa = 8.22FQRR621 pKa = 11.84FDD623 pKa = 3.33ASANRR628 pKa = 11.84VGGEE632 pKa = 3.75VRR634 pKa = 11.84LNTTTASDD642 pKa = 3.32QFTASIAGLSGGGFVATWTSIGQDD666 pKa = 2.65GGTYY670 pKa = 10.33GIYY673 pKa = 10.38AQRR676 pKa = 11.84FDD678 pKa = 3.64SAGNAVGGEE687 pKa = 3.94FRR689 pKa = 11.84VNTITQGDD697 pKa = 3.98QYY699 pKa = 11.11ASRR702 pKa = 11.84VASLPDD708 pKa = 3.24GGFVVAYY715 pKa = 9.32SSSGGEE721 pKa = 4.11DD722 pKa = 3.3GSGIGVFGQRR732 pKa = 11.84YY733 pKa = 8.12DD734 pKa = 3.61AQGTAVGSEE743 pKa = 4.38FIITDD748 pKa = 3.58TTVGNQEE755 pKa = 4.13EE756 pKa = 4.38PHH758 pKa = 5.88LAARR762 pKa = 11.84PDD764 pKa = 3.48GGFVLTWVSPDD775 pKa = 3.0GNGRR779 pKa = 11.84GVFSRR784 pKa = 11.84LVRR787 pKa = 11.84AMAPPSATQAGTAGNDD803 pKa = 3.46LLTPANPRR811 pKa = 11.84SVLLGLAGNDD821 pKa = 3.48TLTGGSLTDD830 pKa = 3.66TLNGGDD836 pKa = 4.46GNDD839 pKa = 3.82SLSGGLGDD847 pKa = 5.22DD848 pKa = 3.92VLFGGIGNDD857 pKa = 3.5TLDD860 pKa = 3.87GGGGADD866 pKa = 4.36LLYY869 pKa = 11.08GGAGNDD875 pKa = 3.47TFIVRR880 pKa = 11.84GGEE883 pKa = 3.99ALSDD887 pKa = 3.21TGGIDD892 pKa = 3.37TAILTGPASYY902 pKa = 9.26TLPGGIEE909 pKa = 4.02NLVLTSAATGTGNSLDD925 pKa = 3.75NLLTGSSGSDD935 pKa = 3.05QLFGLAGNDD944 pKa = 3.56TLDD947 pKa = 3.8GGGGADD953 pKa = 3.67TLIGGLGNDD962 pKa = 3.89TYY964 pKa = 11.11IVDD967 pKa = 4.98DD968 pKa = 4.13IGDD971 pKa = 3.87VVTEE975 pKa = 4.23LSGEE979 pKa = 4.18GADD982 pKa = 4.04EE983 pKa = 4.19VRR985 pKa = 11.84TTLASYY991 pKa = 9.4TLGTNLEE998 pKa = 4.23NLTFTGSGSFTGTGNGLANILQGGAGNDD1026 pKa = 3.81TLDD1029 pKa = 3.78GGAGIDD1035 pKa = 3.6TLRR1038 pKa = 11.84GGVGNDD1044 pKa = 3.19TYY1046 pKa = 11.34LVSDD1050 pKa = 3.76VGDD1053 pKa = 3.84VIIEE1057 pKa = 4.1AVGEE1061 pKa = 4.3GTDD1064 pKa = 3.72EE1065 pKa = 4.28VRR1067 pKa = 11.84TTLASYY1073 pKa = 9.03TLAANVEE1080 pKa = 4.23NLVYY1084 pKa = 10.02TGTVAFTGTGNTGNNAITGGAGNDD1108 pKa = 3.95SLTGDD1113 pKa = 4.3AGNDD1117 pKa = 3.69TLDD1120 pKa = 3.9GGAGADD1126 pKa = 3.6TLVGGTGNDD1135 pKa = 3.43TYY1137 pKa = 11.11IVDD1140 pKa = 3.72TAGDD1144 pKa = 4.07VVTEE1148 pKa = 4.23LAGQGTDD1155 pKa = 3.15TVRR1158 pKa = 11.84TSLASYY1164 pKa = 9.13TLGANVEE1171 pKa = 4.28NLVFTGSGPVTFTGNALNNNLTGGAGADD1199 pKa = 4.07LLTGLDD1205 pKa = 4.67GNDD1208 pKa = 3.75TLNGGAGADD1217 pKa = 3.71TLLGGTGNDD1226 pKa = 3.28TYY1228 pKa = 11.76VVDD1231 pKa = 3.75NVGDD1235 pKa = 3.89VLVEE1239 pKa = 3.98LPGEE1243 pKa = 4.65GIDD1246 pKa = 3.89TVQSSINHH1254 pKa = 5.77TLLGDD1259 pKa = 3.98FEE1261 pKa = 5.41NLTLTGSAALTGTGNALNNLLTGNGAANLLTGLEE1295 pKa = 4.45GDD1297 pKa = 3.88DD1298 pKa = 3.88TLNGGGGADD1307 pKa = 3.62TLIGGLGNDD1316 pKa = 3.84SYY1318 pKa = 12.11VVDD1321 pKa = 3.72NAGDD1325 pKa = 3.74VVTEE1329 pKa = 3.96LVGEE1333 pKa = 4.83GIDD1336 pKa = 3.63TVNASINWTLGTNLEE1351 pKa = 4.13NLTLTGSAAISATGNGLNNLLTGNSGANLLDD1382 pKa = 4.28GGLGADD1388 pKa = 4.05SMVGGAGNDD1397 pKa = 3.43VYY1399 pKa = 11.0IVDD1402 pKa = 4.56DD1403 pKa = 4.64ADD1405 pKa = 4.04DD1406 pKa = 3.92VVTEE1410 pKa = 4.42LVGQGTDD1417 pKa = 3.14EE1418 pKa = 3.99VRR1420 pKa = 11.84TNLSSYY1426 pKa = 11.33LLGTNIEE1433 pKa = 4.44ALTHH1437 pKa = 6.16TGTGDD1442 pKa = 3.36FAGTGNALDD1451 pKa = 3.89NLLTGGLGNDD1461 pKa = 3.86TLSGGDD1467 pKa = 3.84GADD1470 pKa = 3.71TLDD1473 pKa = 4.34GGAGSNLLIGGTGHH1487 pKa = 7.61DD1488 pKa = 3.83VYY1490 pKa = 11.35LVGGFGDD1497 pKa = 3.54QVVEE1501 pKa = 3.93LVGEE1505 pKa = 4.35GMDD1508 pKa = 3.63EE1509 pKa = 4.06VRR1511 pKa = 11.84TALSGYY1517 pKa = 7.4TLTDD1521 pKa = 3.37GVEE1524 pKa = 4.1TLTYY1528 pKa = 9.09TGVAAFVGTGNGLDD1542 pKa = 3.56NLIAGGAGADD1552 pKa = 3.82TLDD1555 pKa = 4.19GGLGADD1561 pKa = 3.88TLIGGLGDD1569 pKa = 3.6DD1570 pKa = 4.24VYY1572 pKa = 11.74VVDD1575 pKa = 4.37SAGDD1579 pKa = 3.77TVIEE1583 pKa = 4.42GADD1586 pKa = 3.23AGLDD1590 pKa = 3.6EE1591 pKa = 5.1VRR1593 pKa = 11.84TTLSAFALGDD1603 pKa = 3.34NTEE1606 pKa = 4.05RR1607 pKa = 11.84LVFQGSGDD1615 pKa = 3.32ASGIGNGLDD1624 pKa = 2.81NWLAGNIGNDD1634 pKa = 3.63TLDD1637 pKa = 3.7GAVGNDD1643 pKa = 3.44TLLGGLGNDD1652 pKa = 3.45VLTGGSGADD1661 pKa = 3.45LFAFAAGDD1669 pKa = 3.9GQDD1672 pKa = 3.72TITDD1676 pKa = 4.08FDD1678 pKa = 4.0AAQGDD1683 pKa = 4.36RR1684 pKa = 11.84IGLAVGQSYY1693 pKa = 11.05SVDD1696 pKa = 3.48TNSNGDD1702 pKa = 3.37AVIVYY1707 pKa = 7.3GTSDD1711 pKa = 2.94IVTLVGIQPAAVSSTWFVTLL1731 pKa = 4.32

Molecular weight: 172.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A431VHE3|A0A431VHE3_9PROT Propionyl-CoA synthetase OS=Azospirillum sp. L-25-5w-1 OX=2496639 GN=EJ903_10570 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 8.96IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.3VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91VLSAA44 pKa = 4.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5160

0

5160

1727630

29

5758

334.8

36.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.313 ± 0.055

0.859 ± 0.01

5.992 ± 0.026

5.027 ± 0.032

3.359 ± 0.024

8.694 ± 0.042

2.077 ± 0.016

4.462 ± 0.026

2.708 ± 0.028

10.82 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.015

2.424 ± 0.019

5.547 ± 0.035

3.012 ± 0.02

7.66 ± 0.039

5.128 ± 0.028

5.66 ± 0.033

7.758 ± 0.026

1.298 ± 0.014

1.939 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski