Amino acid dipepetide frequency for Lactobacillus agilis DSM 20509

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.75AlaAla: 7.75 ± 0.163	0.657AlaCys: 0.657 ± 0.034	5.147AlaAsp: 5.147 ± 0.102	4.93AlaGlu: 4.93 ± 0.103	3.382AlaPhe: 3.382 ± 0.085	6.275AlaGly: 6.275 ± 0.122	1.487AlaHis: 1.487 ± 0.046	5.128AlaIle: 5.128 ± 0.107	7.515AlaLys: 7.515 ± 0.136	9.089AlaLeu: 9.089 ± 0.127	1.945AlaMet: 1.945 ± 0.061	4.373AlaAsn: 4.373 ± 0.097	2.278AlaPro: 2.278 ± 0.069	4.311AlaGln: 4.311 ± 0.098	3.163AlaArg: 3.163 ± 0.077	5.533AlaSer: 5.533 ± 0.118	5.043AlaThr: 5.043 ± 0.123	5.782AlaVal: 5.782 ± 0.1	0.814AlaTrp: 0.814 ± 0.041	3.297AlaTyr: 3.297 ± 0.078	0.0AlaXaa: 0.0 ± 0.0
Cys	0.45CysAla: 0.45 ± 0.027	0.075CysCys: 0.075 ± 0.011	0.393CysAsp: 0.393 ± 0.025	0.309CysGlu: 0.309 ± 0.024	0.353CysPhe: 0.353 ± 0.025	0.612CysGly: 0.612 ± 0.035	0.194CysHis: 0.194 ± 0.021	0.314CysIle: 0.314 ± 0.024	0.239CysLys: 0.239 ± 0.021	0.849CysLeu: 0.849 ± 0.04	0.115CysMet: 0.115 ± 0.013	0.207CysAsn: 0.207 ± 0.019	0.286CysPro: 0.286 ± 0.024	0.369CysGln: 0.369 ± 0.026	0.271CysArg: 0.271 ± 0.024	0.366CysSer: 0.366 ± 0.025	0.242CysThr: 0.242 ± 0.017	0.348CysVal: 0.348 ± 0.025	0.08CysTrp: 0.08 ± 0.012	0.346CysTyr: 0.346 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	3.792AspAla: 3.792 ± 0.09	0.386AspCys: 0.386 ± 0.026	2.736AspAsp: 2.736 ± 0.085	3.982AspGlu: 3.982 ± 0.111	2.929AspPhe: 2.929 ± 0.066	3.62AspGly: 3.62 ± 0.092	0.907AspHis: 0.907 ± 0.044	3.294AspIle: 3.294 ± 0.078	3.797AspLys: 3.797 ± 0.091	6.283AspLeu: 6.283 ± 0.125	1.347AspMet: 1.347 ± 0.052	2.171AspAsn: 2.171 ± 0.058	1.82AspPro: 1.82 ± 0.059	2.401AspGln: 2.401 ± 0.076	1.925AspArg: 1.925 ± 0.061	2.839AspSer: 2.839 ± 0.07	2.441AspThr: 2.441 ± 0.081	3.504AspVal: 3.504 ± 0.077	0.672AspTrp: 0.672 ± 0.037	2.842AspTyr: 2.842 ± 0.079	0.002AspXaa: 0.002 ± 0.002
Glu	5.481GluAla: 5.481 ± 0.112	0.286GluCys: 0.286 ± 0.02	2.866GluAsp: 2.866 ± 0.085	4.231GluGlu: 4.231 ± 0.104	2.356GluPhe: 2.356 ± 0.067	3.086GluGly: 3.086 ± 0.082	1.039GluHis: 1.039 ± 0.042	4.405GluIle: 4.405 ± 0.092	4.351GluLys: 4.351 ± 0.104	7.08GluLeu: 7.08 ± 0.139	1.778GluMet: 1.778 ± 0.061	2.737GluAsn: 2.737 ± 0.079	1.531GluPro: 1.531 ± 0.052	3.035GluGln: 3.035 ± 0.085	2.684GluArg: 2.684 ± 0.071	2.609GluSer: 2.609 ± 0.075	2.973GluThr: 2.973 ± 0.073	4.783GluVal: 4.783 ± 0.099	0.523GluTrp: 0.523 ± 0.033	1.957GluTyr: 1.957 ± 0.07	0.002GluXaa: 0.002 ± 0.001
Phe	3.374PheAla: 3.374 ± 0.078	0.323PheCys: 0.323 ± 0.022	2.647PheAsp: 2.647 ± 0.07	2.523PheGlu: 2.523 ± 0.069	1.995PhePhe: 1.995 ± 0.078	3.016PheGly: 3.016 ± 0.079	0.655PheHis: 0.655 ± 0.031	2.943PheIle: 2.943 ± 0.085	3.025PheLys: 3.025 ± 0.078	4.026PheLeu: 4.026 ± 0.103	1.106PheMet: 1.106 ± 0.041	2.199PheAsn: 2.199 ± 0.059	1.355PhePro: 1.355 ± 0.047	1.186PheGln: 1.186 ± 0.041	1.277PheArg: 1.277 ± 0.048	2.782PheSer: 2.782 ± 0.076	2.415PheThr: 2.415 ± 0.061	2.884PheVal: 2.884 ± 0.07	0.45PheTrp: 0.45 ± 0.028	1.751PheTyr: 1.751 ± 0.062	0.0PheXaa: 0.0 ± 0.0
Gly	4.96GlyAla: 4.96 ± 0.102	0.473GlyCys: 0.473 ± 0.025	3.195GlyAsp: 3.195 ± 0.075	3.12GlyGlu: 3.12 ± 0.074	3.016GlyPhe: 3.016 ± 0.075	4.261GlyGly: 4.261 ± 0.086	1.369GlyHis: 1.369 ± 0.051	5.087GlyIle: 5.087 ± 0.106	4.986GlyLys: 4.986 ± 0.095	7.384GlyLeu: 7.384 ± 0.115	1.735GlyMet: 1.735 ± 0.051	2.674GlyAsn: 2.674 ± 0.08	1.594GlyPro: 1.594 ± 0.052	3.762GlyGln: 3.762 ± 0.107	2.659GlyArg: 2.659 ± 0.075	3.691GlySer: 3.691 ± 0.081	3.695GlyThr: 3.695 ± 0.088	4.659GlyVal: 4.659 ± 0.099	0.673GlyTrp: 0.673 ± 0.036	2.873GlyTyr: 2.873 ± 0.075	0.005GlyXaa: 0.005 ± 0.003
His	1.141HisAla: 1.141 ± 0.041	0.13HisCys: 0.13 ± 0.015	0.974HisAsp: 0.974 ± 0.043	0.963HisGlu: 0.963 ± 0.043	0.984HisPhe: 0.984 ± 0.038	1.307HisGly: 1.307 ± 0.05	0.536HisHis: 0.536 ± 0.034	0.981HisIle: 0.981 ± 0.044	1.073HisLys: 1.073 ± 0.04	1.967HisLeu: 1.967 ± 0.066	0.366HisMet: 0.366 ± 0.023	0.804HisAsn: 0.804 ± 0.034	0.924HisPro: 0.924 ± 0.038	0.941HisGln: 0.941 ± 0.041	0.754HisArg: 0.754 ± 0.035	0.916HisSer: 0.916 ± 0.042	0.852HisThr: 0.852 ± 0.042	1.068HisVal: 1.068 ± 0.042	0.214HisTrp: 0.214 ± 0.027	0.924HisTyr: 0.924 ± 0.041	0.0HisXaa: 0.0 ± 0.0
Ile	5.543IleAla: 5.543 ± 0.101	0.592IleCys: 0.592 ± 0.037	3.623IleAsp: 3.623 ± 0.082	3.81IleGlu: 3.81 ± 0.092	2.704IlePhe: 2.704 ± 0.077	4.44IleGly: 4.44 ± 0.109	1.061IleHis: 1.061 ± 0.042	4.57IleIle: 4.57 ± 0.125	4.702IleLys: 4.702 ± 0.104	6.128IleLeu: 6.128 ± 0.129	1.609IleMet: 1.609 ± 0.052	3.063IleAsn: 3.063 ± 0.082	2.47IlePro: 2.47 ± 0.072	1.98IleGln: 1.98 ± 0.059	2.279IleArg: 2.279 ± 0.06	4.363IleSer: 4.363 ± 0.094	3.621IleThr: 3.621 ± 0.075	4.509IleVal: 4.509 ± 0.085	0.513IleTrp: 0.513 ± 0.032	2.37IleTyr: 2.37 ± 0.073	0.0IleXaa: 0.0 ± 0.0
Lys	6.251LysAla: 6.251 ± 0.106	0.286LysCys: 0.286 ± 0.023	3.882LysAsp: 3.882 ± 0.08	5.311LysGlu: 5.311 ± 0.091	2.323LysPhe: 2.323 ± 0.067	3.588LysGly: 3.588 ± 0.082	1.307LysHis: 1.307 ± 0.055	4.323LysIle: 4.323 ± 0.081	5.668LysLys: 5.668 ± 0.123	7.827LysLeu: 7.827 ± 0.133	2.062LysMet: 2.062 ± 0.068	3.213LysAsn: 3.213 ± 0.079	2.264LysPro: 2.264 ± 0.064	4.468LysGln: 4.468 ± 0.104	3.675LysArg: 3.675 ± 0.094	3.163LysSer: 3.163 ± 0.08	4.119LysThr: 4.119 ± 0.091	5.374LysVal: 5.374 ± 0.117	0.71LysTrp: 0.71 ± 0.041	2.585LysTyr: 2.585 ± 0.069	0.003LysXaa: 0.003 ± 0.002
Leu	11.98LeuAla: 11.98 ± 0.191	0.677LeuCys: 0.677 ± 0.034	5.83LeuAsp: 5.83 ± 0.123	6.258LeuGlu: 6.258 ± 0.111	3.999LeuPhe: 3.999 ± 0.099	7.62LeuGly: 7.62 ± 0.135	1.638LeuHis: 1.638 ± 0.051	6.462LeuIle: 6.462 ± 0.13	7.608LeuLys: 7.608 ± 0.127	10.335LeuLeu: 10.335 ± 0.214	2.426LeuMet: 2.426 ± 0.069	4.773LeuAsn: 4.773 ± 0.105	4.154LeuPro: 4.154 ± 0.089	3.553LeuGln: 3.553 ± 0.089	4.089LeuArg: 4.089 ± 0.095	6.203LeuSer: 6.203 ± 0.096	7.199LeuThr: 7.199 ± 0.117	8.091LeuVal: 8.091 ± 0.129	0.836LeuTrp: 0.836 ± 0.042	3.147LeuTyr: 3.147 ± 0.082	0.0LeuXaa: 0.0 ± 0.0
Met	2.271MetAla: 2.271 ± 0.063	0.127MetCys: 0.127 ± 0.013	1.257MetAsp: 1.257 ± 0.043	1.392MetGlu: 1.392 ± 0.042	0.815MetPhe: 0.815 ± 0.036	1.648MetGly: 1.648 ± 0.054	0.364MetHis: 0.364 ± 0.025	1.669MetIle: 1.669 ± 0.054	1.855MetLys: 1.855 ± 0.054	2.319MetLeu: 2.319 ± 0.067	0.65MetMet: 0.65 ± 0.033	1.071MetAsn: 1.071 ± 0.043	0.922MetPro: 0.922 ± 0.035	1.103MetGln: 1.103 ± 0.042	0.934MetArg: 0.934 ± 0.036	1.344MetSer: 1.344 ± 0.045	1.648MetThr: 1.648 ± 0.055	1.796MetVal: 1.796 ± 0.053	0.155MetTrp: 0.155 ± 0.015	0.675MetTyr: 0.675 ± 0.03	0.002MetXaa: 0.002 ± 0.002
Asn	3.053AsnAla: 3.053 ± 0.078	0.291AsnCys: 0.291 ± 0.023	2.48AsnAsp: 2.48 ± 0.064	2.695AsnGlu: 2.695 ± 0.07	2.109AsnPhe: 2.109 ± 0.065	3.235AsnGly: 3.235 ± 0.081	1.009AsnHis: 1.009 ± 0.043	2.714AsnIle: 2.714 ± 0.063	2.888AsnLys: 2.888 ± 0.081	4.983AsnLeu: 4.983 ± 0.112	1.091AsnMet: 1.091 ± 0.047	2.226AsnAsn: 2.226 ± 0.074	2.162AsnPro: 2.162 ± 0.065	2.548AsnGln: 2.548 ± 0.078	1.796AsnArg: 1.796 ± 0.058	2.547AsnSer: 2.547 ± 0.076	2.151AsnThr: 2.151 ± 0.058	2.829AsnVal: 2.829 ± 0.074	0.618AsnTrp: 0.618 ± 0.031	2.164AsnTyr: 2.164 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	3.133ProAla: 3.133 ± 0.082	0.17ProCys: 0.17 ± 0.015	1.903ProAsp: 1.903 ± 0.057	2.396ProGlu: 2.396 ± 0.072	1.552ProPhe: 1.552 ± 0.046	2.069ProGly: 2.069 ± 0.062	0.613ProHis: 0.613 ± 0.035	1.852ProIle: 1.852 ± 0.059	2.65ProLys: 2.65 ± 0.06	3.563ProLeu: 3.563 ± 0.087	0.61ProMet: 0.61 ± 0.033	1.706ProAsn: 1.706 ± 0.057	0.622ProPro: 0.622 ± 0.034	1.414ProGln: 1.414 ± 0.051	1.085ProArg: 1.085 ± 0.045	2.044ProSer: 2.044 ± 0.063	2.187ProThr: 2.187 ± 0.057	2.64ProVal: 2.64 ± 0.071	0.323ProTrp: 0.323 ± 0.023	1.397ProTyr: 1.397 ± 0.061	0.0ProXaa: 0.0 ± 0.0
Gln	6.231GlnAla: 6.231 ± 0.149	0.147GlnCys: 0.147 ± 0.016	1.89GlnAsp: 1.89 ± 0.052	2.644GlnGlu: 2.644 ± 0.073	1.362GlnPhe: 1.362 ± 0.047	2.57GlnGly: 2.57 ± 0.068	0.675GlnHis: 0.675 ± 0.033	2.804GlnIle: 2.804 ± 0.074	3.285GlnLys: 3.285 ± 0.091	5.24GlnLeu: 5.24 ± 0.118	1.1GlnMet: 1.1 ± 0.05	1.892GlnAsn: 1.892 ± 0.061	1.462GlnPro: 1.462 ± 0.056	1.969GlnGln: 1.969 ± 0.078	1.863GlnArg: 1.863 ± 0.067	2.07GlnSer: 2.07 ± 0.064	3.078GlnThr: 3.078 ± 0.081	4.149GlnVal: 4.149 ± 0.102	0.314GlnTrp: 0.314 ± 0.023	1.409GlnTyr: 1.409 ± 0.05	0.0GlnXaa: 0.0 ± 0.0
Arg	3.031ArgAla: 3.031 ± 0.076	0.206ArgCys: 0.206 ± 0.019	1.907ArgAsp: 1.907 ± 0.066	2.244ArgGlu: 2.244 ± 0.066	1.785ArgPhe: 1.785 ± 0.051	2.099ArgGly: 2.099 ± 0.064	0.755ArgHis: 0.755 ± 0.032	2.609ArgIle: 2.609 ± 0.062	2.979ArgLys: 2.979 ± 0.076	4.52ArgLeu: 4.52 ± 0.103	0.966ArgMet: 0.966 ± 0.042	1.783ArgAsn: 1.783 ± 0.058	1.454ArgPro: 1.454 ± 0.048	2.344ArgGln: 2.344 ± 0.068	1.985ArgArg: 1.985 ± 0.061	1.833ArgSer: 1.833 ± 0.058	1.918ArgThr: 1.918 ± 0.055	2.622ArgVal: 2.622 ± 0.073	0.338ArgTrp: 0.338 ± 0.024	1.823ArgTyr: 1.823 ± 0.063	0.005ArgXaa: 0.005 ± 0.003
Ser	4.123SerAla: 4.123 ± 0.095	0.331SerCys: 0.331 ± 0.024	3.108SerAsp: 3.108 ± 0.077	3.327SerGlu: 3.327 ± 0.074	2.699SerPhe: 2.699 ± 0.074	4.037SerGly: 4.037 ± 0.093	1.101SerHis: 1.101 ± 0.044	3.16SerIle: 3.16 ± 0.072	3.852SerLys: 3.852 ± 0.09	6.805SerLeu: 6.805 ± 0.125	1.272SerMet: 1.272 ± 0.045	2.366SerAsn: 2.366 ± 0.075	2.034SerPro: 2.034 ± 0.05	3.32SerGln: 3.32 ± 0.09	2.319SerArg: 2.319 ± 0.058	3.716SerSer: 3.716 ± 0.138	2.674SerThr: 2.674 ± 0.07	3.583SerVal: 3.583 ± 0.088	0.695SerTrp: 0.695 ± 0.036	2.428SerTyr: 2.428 ± 0.074	0.0SerXaa: 0.0 ± 0.0
Thr	4.843ThrAla: 4.843 ± 0.091	0.306ThrCys: 0.306 ± 0.021	3.175ThrAsp: 3.175 ± 0.077	3.081ThrGlu: 3.081 ± 0.078	2.378ThrPhe: 2.378 ± 0.066	4.285ThrGly: 4.285 ± 0.108	0.922ThrHis: 0.922 ± 0.044	3.875ThrIle: 3.875 ± 0.092	4.433ThrLys: 4.433 ± 0.079	5.458ThrLeu: 5.458 ± 0.101	1.253ThrMet: 1.253 ± 0.047	2.924ThrAsn: 2.924 ± 0.067	2.358ThrPro: 2.358 ± 0.067	1.775ThrGln: 1.775 ± 0.06	1.654ThrArg: 1.654 ± 0.062	3.636ThrSer: 3.636 ± 0.083	3.579ThrThr: 3.579 ± 0.099	4.253ThrVal: 4.253 ± 0.098	0.55ThrTrp: 0.55 ± 0.032	2.356ThrTyr: 2.356 ± 0.069	0.002ThrXaa: 0.002 ± 0.002
Val	7.105ValAla: 7.105 ± 0.119	0.568ValCys: 0.568 ± 0.031	4.017ValAsp: 4.017 ± 0.087	4.046ValGlu: 4.046 ± 0.09	2.704ValPhe: 2.704 ± 0.069	4.839ValGly: 4.839 ± 0.112	1.031ValHis: 1.031 ± 0.036	5.197ValIle: 5.197 ± 0.109	5.239ValLys: 5.239 ± 0.104	6.81ValLeu: 6.81 ± 0.097	1.561ValMet: 1.561 ± 0.058	3.232ValAsn: 3.232 ± 0.065	2.38ValPro: 2.38 ± 0.071	2.084ValGln: 2.084 ± 0.063	2.49ValArg: 2.49 ± 0.063	4.54ValSer: 4.54 ± 0.091	4.866ValThr: 4.866 ± 0.089	5.504ValVal: 5.504 ± 0.121	0.603ValTrp: 0.603 ± 0.031	2.435ValTyr: 2.435 ± 0.076	0.003ValXaa: 0.003 ± 0.003
Trp	0.737TrpAla: 0.737 ± 0.038	0.072TrpCys: 0.072 ± 0.011	0.49TrpAsp: 0.49 ± 0.032	0.414TrpGlu: 0.414 ± 0.029	0.426TrpPhe: 0.426 ± 0.029	0.613TrpGly: 0.613 ± 0.037	0.197TrpHis: 0.197 ± 0.02	0.51TrpIle: 0.51 ± 0.031	0.461TrpLys: 0.461 ± 0.034	1.355TrpLeu: 1.355 ± 0.055	0.281TrpMet: 0.281 ± 0.023	0.466TrpAsn: 0.466 ± 0.029	0.287TrpPro: 0.287 ± 0.022	0.782TrpGln: 0.782 ± 0.041	0.404TrpArg: 0.404 ± 0.026	0.536TrpSer: 0.536 ± 0.037	0.475TrpThr: 0.475 ± 0.033	0.577TrpVal: 0.577 ± 0.032	0.119TrpTrp: 0.119 ± 0.013	0.428TrpTyr: 0.428 ± 0.036	0.002TrpXaa: 0.002 ± 0.002
Tyr	2.649TyrAla: 2.649 ± 0.074	0.316TyrCys: 0.316 ± 0.022	2.167TyrAsp: 2.167 ± 0.059	2.117TyrGlu: 2.117 ± 0.069	2.06TyrPhe: 2.06 ± 0.063	2.65TyrGly: 2.65 ± 0.079	0.953TyrHis: 0.953 ± 0.046	2.054TyrIle: 2.054 ± 0.064	1.795TyrLys: 1.795 ± 0.055	4.854TyrLeu: 4.854 ± 0.101	0.742TyrMet: 0.742 ± 0.036	1.708TyrAsn: 1.708 ± 0.066	1.572TyrPro: 1.572 ± 0.054	2.809TyrGln: 2.809 ± 0.084	1.898TyrArg: 1.898 ± 0.059	2.217TyrSer: 2.217 ± 0.069	1.918TyrThr: 1.918 ± 0.061	2.296TyrVal: 2.296 ± 0.059	0.431TyrTrp: 0.431 ± 0.028	1.952TyrTyr: 1.952 ± 0.075	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.003XaaAsp: 0.003 ± 0.002	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.003XaaHis: 0.003 ± 0.003	0.0XaaIle: 0.0 ± 0.0	0.002XaaLys: 0.002 ± 0.002	0.002XaaLeu: 0.002 ± 0.002	0.002XaaMet: 0.002 ± 0.002	0.002XaaAsn: 0.002 ± 0.002	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.002	0.003XaaVal: 0.003 ± 0.002	0.002XaaTrp: 0.002 ± 0.002	0.002XaaTyr: 0.002 ± 0.002	0.336XaaXaa: 0.336 ± 0.122

Statistics based on 1951 proteins (598424 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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