Nadsonia fulvescens var. elongata DSM 6958

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetales incertae sedis; Nadsonia; Nadsonia fulvescens; Nadsonia fulvescens var. elongata

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5636 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3PGC5|A0A1E3PGC5_9ASCO MHD domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 OX=857566 GN=NADFUDRAFT_47147 PE=4 SV=1
MM1 pKa = 8.05AITITNSRR9 pKa = 11.84PSMADD14 pKa = 3.19YY15 pKa = 10.16TPLAQYY21 pKa = 9.4QASTPASYY29 pKa = 11.21SLDD32 pKa = 3.74EE33 pKa = 4.15NPILHH38 pKa = 6.72FSYY41 pKa = 10.7AGAGLTSSLDD51 pKa = 3.79LPTHH55 pKa = 5.34PTIISKK61 pKa = 9.43TSTATDD67 pKa = 3.17IYY69 pKa = 11.25VLNTDD74 pKa = 3.97LVFWFPNQEE83 pKa = 4.11CGLSVSYY90 pKa = 10.71PDD92 pKa = 4.83IILHH96 pKa = 7.4AIQTQQSPSIYY107 pKa = 10.17LQINFQLGFQGSITEE122 pKa = 4.25SDD124 pKa = 3.36LTDD127 pKa = 5.45DD128 pKa = 3.82YY129 pKa = 11.92DD130 pKa = 4.56NPILEE135 pKa = 4.67LTITMSPEE143 pKa = 3.55DD144 pKa = 4.06QLRR147 pKa = 11.84KK148 pKa = 9.87LYY150 pKa = 10.69SALSHH155 pKa = 6.44CASLHH160 pKa = 6.45PDD162 pKa = 3.61FDD164 pKa = 6.02SDD166 pKa = 4.8DD167 pKa = 3.96QEE169 pKa = 4.18QGAGDD174 pKa = 4.22EE175 pKa = 4.27SDD177 pKa = 4.03SYY179 pKa = 11.79YY180 pKa = 11.38DD181 pKa = 3.57NLEE184 pKa = 4.67DD185 pKa = 4.19EE186 pKa = 5.01DD187 pKa = 5.32AFPEE191 pKa = 3.94DD192 pKa = 4.17HH193 pKa = 6.63QWITAEE199 pKa = 4.96NIDD202 pKa = 3.7SMEE205 pKa = 4.47FGNHH209 pKa = 4.87ASAALEE215 pKa = 4.2VVLDD219 pKa = 4.15EE220 pKa = 5.11PIKK223 pKa = 9.45TGIVRR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84EE231 pKa = 4.2SIEE234 pKa = 4.27AEE236 pKa = 3.75ADD238 pKa = 3.35QEE240 pKa = 4.62AGYY243 pKa = 10.88DD244 pKa = 3.64DD245 pKa = 4.73SNNAFNQKK253 pKa = 10.53DD254 pKa = 3.67DD255 pKa = 3.68TVKK258 pKa = 9.34WRR260 pKa = 11.84RR261 pKa = 11.84MEE263 pKa = 3.86

Molecular weight: 29.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3PKN5|A0A1E3PKN5_9ASCO SET domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 OX=857566 GN=NADFUDRAFT_41974 PE=4 SV=1
MM1 pKa = 7.43FNHH4 pKa = 6.89LSVGLRR10 pKa = 11.84TVPASSRR17 pKa = 11.84FLMGSRR23 pKa = 11.84AFSYY27 pKa = 10.65VPRR30 pKa = 11.84SALTAFRR37 pKa = 11.84AATPSTLNSSLAGRR51 pKa = 11.84EE52 pKa = 4.0STANIAMGSPLLAVPTMGLMSSLAGALQVRR82 pKa = 11.84WKK84 pKa = 10.86SRR86 pKa = 11.84GHH88 pKa = 5.52TFQPNTLKK96 pKa = 10.71RR97 pKa = 11.84KK98 pKa = 9.5RR99 pKa = 11.84KK100 pKa = 9.24FGFLAKK106 pKa = 9.42MRR108 pKa = 11.84AKK110 pKa = 10.18LGRR113 pKa = 11.84VIISRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84AKK122 pKa = 10.25GRR124 pKa = 11.84WYY126 pKa = 10.54LSHH129 pKa = 7.13

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5635

1

5636

2669257

49

5015

473.6

52.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.618 ± 0.034

1.138 ± 0.011

5.804 ± 0.024

6.216 ± 0.033

4.087 ± 0.021

5.6 ± 0.028

2.188 ± 0.016

6.368 ± 0.021

6.19 ± 0.027

9.22 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.023 ± 0.01

5.57 ± 0.03

4.737 ± 0.027

3.924 ± 0.034

4.749 ± 0.021

9.508 ± 0.05

5.938 ± 0.024

5.927 ± 0.023

1.055 ± 0.011

3.134 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski