Streptomyces phage Mildred21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samistivirus; Streptomyces virus Mildred21

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222YX35|A0A222YX35_9CAUD Uncharacterized protein OS=Streptomyces phage Mildred21 OX=2023959 GN=SEA_MILDRED21_230 PE=4 SV=1
MM1 pKa = 7.15GWAGGTSYY9 pKa = 11.36FDD11 pKa = 3.91GPLDD15 pKa = 4.15LMLEE19 pKa = 4.25YY20 pKa = 10.94VPEE23 pKa = 4.57DD24 pKa = 3.12KK25 pKa = 10.6RR26 pKa = 11.84KK27 pKa = 9.3EE28 pKa = 4.23VIEE31 pKa = 3.89KK32 pKa = 10.05LYY34 pKa = 10.59WEE36 pKa = 4.71IRR38 pKa = 11.84DD39 pKa = 4.05GDD41 pKa = 3.98WDD43 pKa = 4.13TVDD46 pKa = 3.28EE47 pKa = 4.29SAYY50 pKa = 10.74FNLLVKK56 pKa = 10.55YY57 pKa = 10.44DD58 pKa = 3.25IDD60 pKa = 4.88GYY62 pKa = 11.24GEE64 pKa = 4.11YY65 pKa = 10.74KK66 pKa = 10.44NDD68 pKa = 3.83PDD70 pKa = 4.5YY71 pKa = 11.63LEE73 pKa = 4.04QLNEE77 pKa = 4.01EE78 pKa = 4.39EE79 pKa = 4.3LAEE82 pKa = 4.34LNEE85 pKa = 4.23

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222YU79|A0A222YU79_9CAUD Uncharacterized protein OS=Streptomyces phage Mildred21 OX=2023959 GN=SEA_MILDRED21_137 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84FAFGLRR8 pKa = 11.84PGHH11 pKa = 4.69VTKK14 pKa = 10.62HH15 pKa = 4.37GTVVAVQKK23 pKa = 9.84TFDD26 pKa = 4.04GKK28 pKa = 10.3HH29 pKa = 4.95MQLTLAGRR37 pKa = 11.84SDD39 pKa = 3.45NVVISKK45 pKa = 7.66YY46 pKa = 9.04TIVRR50 pKa = 11.84TTFWSS55 pKa = 3.35

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

212

0

212

34506

31

2104

162.8

18.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.43 ± 0.341

1.09 ± 0.117

6.59 ± 0.184

7.1 ± 0.345

3.86 ± 0.13

7.405 ± 0.22

1.936 ± 0.131

5.332 ± 0.146

6.422 ± 0.3

7.158 ± 0.184

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.124

4.425 ± 0.155

3.767 ± 0.163

3.127 ± 0.191

5.785 ± 0.196

5.924 ± 0.275

6.063 ± 0.433

7.158 ± 0.192

1.89 ± 0.118

3.715 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski