Oceanicaulis sp. HTCC2633

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Maricaulales; Maricaulaceae; Oceanicaulis; unclassified Oceanicaulis

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3UEP8|A3UEP8_9PROT Membrane protein putative OS=Oceanicaulis sp. HTCC2633 OX=314254 GN=OA2633_11660 PE=4 SV=1
MM1 pKa = 7.06TSKK4 pKa = 11.16LILSVKK10 pKa = 10.25GLVLFAWLSLFSATALAQDD29 pKa = 4.75LGSSQSIDD37 pKa = 3.28FNAAGFPDD45 pKa = 4.25PALLLGKK52 pKa = 9.34PYY54 pKa = 10.75ANSGFTFDD62 pKa = 4.46YY63 pKa = 10.96SGNLYY68 pKa = 10.95GLDD71 pKa = 3.66EE72 pKa = 4.73SDD74 pKa = 3.95ARR76 pKa = 11.84QGKK79 pKa = 9.01LWVQSATQDD88 pKa = 3.2EE89 pKa = 5.49TFTIDD94 pKa = 3.39YY95 pKa = 10.92SGDD98 pKa = 3.63NFDD101 pKa = 4.97LNSFILVNGFSTYY114 pKa = 10.66NITVRR119 pKa = 11.84GYY121 pKa = 9.45DD122 pKa = 3.09ASNSVVEE129 pKa = 4.42TQSFSVPSGTQTISLTNSGFNNVRR153 pKa = 11.84YY154 pKa = 10.06VIITATSANGFDD166 pKa = 4.49GSFFDD171 pKa = 5.38DD172 pKa = 4.2FAVAPPTVPDD182 pKa = 3.32VTAPRR187 pKa = 11.84VTSIVRR193 pKa = 11.84QTPATAITSVDD204 pKa = 3.38SLVFRR209 pKa = 11.84VTFDD213 pKa = 3.36EE214 pKa = 4.5AVQNVSSADD223 pKa = 3.51FTVTGVSGAALGVSGSSAVYY243 pKa = 10.39DD244 pKa = 3.46ITVSGGDD251 pKa = 3.34TASYY255 pKa = 9.58TGTVSLGFRR264 pKa = 11.84GTQDD268 pKa = 3.27IADD271 pKa = 4.13TPAGNTLVNTTPTGANEE288 pKa = 4.93SYY290 pKa = 10.16TLDD293 pKa = 3.51NTAPAAPSTPDD304 pKa = 3.5LDD306 pKa = 4.63AGSDD310 pKa = 3.56TGSSNTDD317 pKa = 3.15NITNDD322 pKa = 3.38TTPTFTGTAEE332 pKa = 4.02ANATVTVSSTLSGPLGSTTADD353 pKa = 3.3GSGNWSYY360 pKa = 11.53TSGVLAEE367 pKa = 4.45GAHH370 pKa = 6.54TITATATDD378 pKa = 3.48AASNVSAASSALSITIDD395 pKa = 3.23TTAPDD400 pKa = 3.17ITSITRR406 pKa = 11.84LSPTGQTISASAANAAFRR424 pKa = 11.84VTYY427 pKa = 10.38SEE429 pKa = 5.21AIAGSSQADD438 pKa = 3.4YY439 pKa = 11.51AFQNVSGTAAGSVSSYY455 pKa = 9.06GTSSPTLINVQLGTLSGDD473 pKa = 3.27GDD475 pKa = 4.11FSITLNGGHH484 pKa = 7.69GITDD488 pKa = 3.32IAGNALSSTTPTGVRR503 pKa = 11.84EE504 pKa = 3.99VFTRR508 pKa = 11.84DD509 pKa = 2.64GTAPSTTSFVRR520 pKa = 11.84TTPAGATTSSDD531 pKa = 3.43SLVFTANYY539 pKa = 9.01SEE541 pKa = 4.58SVSGVDD547 pKa = 3.14TSDD550 pKa = 3.58FAVTGTTATVQSVSAASGTSIAITVSGGDD579 pKa = 3.28LASYY583 pKa = 10.6NGTVGLNYY591 pKa = 10.18AAAPTITDD599 pKa = 3.39AAGNALPNTEE609 pKa = 4.15PATDD613 pKa = 3.39EE614 pKa = 4.7TYY616 pKa = 10.8TLDD619 pKa = 3.35NTAPAGYY626 pKa = 9.95SVTLDD631 pKa = 4.21DD632 pKa = 5.86DD633 pKa = 4.03PVNAGNQTATSFTFAGAEE651 pKa = 3.86VAANYY656 pKa = 10.46NYY658 pKa = 9.9TISSSGGGANVTGSGTISTATDD680 pKa = 3.44QISGIDD686 pKa = 3.59VSGLGDD692 pKa = 3.55GTLTLSVTLTDD703 pKa = 3.87GAGNAGSAATDD714 pKa = 3.54TATKK718 pKa = 9.71EE719 pKa = 4.05AQPPQVNSIVVAGSPAGNATSVDD742 pKa = 4.01FTVTFSEE749 pKa = 4.3AAQNISTDD757 pKa = 3.94DD758 pKa = 3.78FSLTTTGTATGTIASVSSATGTTVTVTVNTISGDD792 pKa = 3.36GTIRR796 pKa = 11.84LDD798 pKa = 4.33LDD800 pKa = 3.65AATNIQDD807 pKa = 3.54AVGNSGPNAYY817 pKa = 9.16TSGATHH823 pKa = 6.31TVDD826 pKa = 3.12NTAPAAFNVAFQAGLITQANEE847 pKa = 3.6TSNDD851 pKa = 2.81IRR853 pKa = 11.84FTTAEE858 pKa = 3.94TGTTYY863 pKa = 10.69AYY865 pKa = 10.4SISSSGGGTPVTGTGTITTATQDD888 pKa = 3.12VTVNVSSLPDD898 pKa = 3.38GTLTLTATLTDD909 pKa = 3.55GAGNVTNQSSADD921 pKa = 3.81TVSKK925 pKa = 9.79ATAVPGFSMAFAPTSVLTGGTSTATT950 pKa = 3.33

Molecular weight: 94.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3UC48|A3UC48_9PROT Uncharacterized protein OS=Oceanicaulis sp. HTCC2633 OX=314254 GN=OA2633_01606 PE=4 SV=1
SS1 pKa = 7.65PMRR4 pKa = 11.84QNMKK8 pKa = 9.59IRR10 pKa = 11.84RR11 pKa = 11.84AMPMVAVVRR20 pKa = 11.84KK21 pKa = 9.27AQARR25 pKa = 11.84LL26 pKa = 3.28

Molecular weight: 3.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3028

0

3028

953775

23

2187

315.0

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.869 ± 0.059

0.746 ± 0.014

6.38 ± 0.036

6.581 ± 0.05

3.67 ± 0.028

8.505 ± 0.04

1.872 ± 0.027

4.705 ± 0.026

2.832 ± 0.038

10.161 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.023

2.551 ± 0.03

4.958 ± 0.033

3.323 ± 0.027

7.004 ± 0.042

5.643 ± 0.035

5.114 ± 0.039

7.14 ± 0.033

1.318 ± 0.018

2.215 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski