Amino acid dipepetide frequency for Microbacteriaceae bacterium MWH-Ta3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.972AlaAla: 16.972 ± 0.279	0.541AlaCys: 0.541 ± 0.036	7.392AlaAsp: 7.392 ± 0.138	7.795AlaGlu: 7.795 ± 0.151	3.735AlaPhe: 3.735 ± 0.096	10.589AlaGly: 10.589 ± 0.178	2.912AlaHis: 2.912 ± 0.09	6.759AlaIle: 6.759 ± 0.133	3.205AlaLys: 3.205 ± 0.102	13.055AlaLeu: 13.055 ± 0.221	2.881AlaMet: 2.881 ± 0.077	3.211AlaAsn: 3.211 ± 0.087	4.953AlaPro: 4.953 ± 0.118	3.954AlaGln: 3.954 ± 0.097	8.057AlaArg: 8.057 ± 0.148	6.67AlaSer: 6.67 ± 0.118	7.64AlaThr: 7.64 ± 0.127	10.674AlaVal: 10.674 ± 0.175	1.597AlaTrp: 1.597 ± 0.061	2.359AlaTyr: 2.359 ± 0.081	0.0AlaXaa: 0.0 ± 0.0
Cys	0.828CysAla: 0.828 ± 0.042	0.062CysCys: 0.062 ± 0.011	0.411CysAsp: 0.411 ± 0.032	0.349CysGlu: 0.349 ± 0.03	0.151CysPhe: 0.151 ± 0.019	0.582CysGly: 0.582 ± 0.038	0.136CysHis: 0.136 ± 0.016	0.206CysIle: 0.206 ± 0.024	0.068CysLys: 0.068 ± 0.014	0.402CysLeu: 0.402 ± 0.03	0.105CysMet: 0.105 ± 0.015	0.134CysAsn: 0.134 ± 0.017	0.316CysPro: 0.316 ± 0.024	0.13CysGln: 0.13 ± 0.018	0.318CysArg: 0.318 ± 0.025	0.305CysSer: 0.305 ± 0.025	0.365CysThr: 0.365 ± 0.035	0.477CysVal: 0.477 ± 0.03	0.054CysTrp: 0.054 ± 0.011	0.114CysTyr: 0.114 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.632AspAla: 7.632 ± 0.135	0.316AspCys: 0.316 ± 0.029	4.02AspAsp: 4.02 ± 0.124	4.21AspGlu: 4.21 ± 0.106	2.175AspPhe: 2.175 ± 0.077	5.265AspGly: 5.265 ± 0.148	1.145AspHis: 1.145 ± 0.054	3.781AspIle: 3.781 ± 0.082	1.344AspLys: 1.344 ± 0.064	5.417AspLeu: 5.417 ± 0.118	1.271AspMet: 1.271 ± 0.052	1.533AspAsn: 1.533 ± 0.057	3.232AspPro: 3.232 ± 0.083	1.253AspGln: 1.253 ± 0.056	3.876AspArg: 3.876 ± 0.122	3.936AspSer: 3.936 ± 0.083	3.515AspThr: 3.515 ± 0.084	5.518AspVal: 5.518 ± 0.108	0.811AspTrp: 0.811 ± 0.04	1.531AspTyr: 1.531 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	6.973GluAla: 6.973 ± 0.138	0.367GluCys: 0.367 ± 0.03	2.427GluAsp: 2.427 ± 0.082	2.596GluGlu: 2.596 ± 0.095	2.276GluPhe: 2.276 ± 0.073	3.793GluGly: 3.793 ± 0.093	1.407GluHis: 1.407 ± 0.056	3.552GluIle: 3.552 ± 0.088	1.723GluLys: 1.723 ± 0.079	6.22GluLeu: 6.22 ± 0.143	1.275GluMet: 1.275 ± 0.055	1.628GluAsn: 1.628 ± 0.062	2.637GluPro: 2.637 ± 0.1	1.756GluGln: 1.756 ± 0.061	4.462GluArg: 4.462 ± 0.126	3.923GluSer: 3.923 ± 0.089	3.197GluThr: 3.197 ± 0.085	4.584GluVal: 4.584 ± 0.1	1.075GluTrp: 1.075 ± 0.048	1.35GluTyr: 1.35 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	4.643PheAla: 4.643 ± 0.115	0.169PheCys: 0.169 ± 0.018	2.681PheAsp: 2.681 ± 0.082	1.911PheGlu: 1.911 ± 0.068	1.133PhePhe: 1.133 ± 0.053	3.618PheGly: 3.618 ± 0.096	0.675PheHis: 0.675 ± 0.035	1.692PheIle: 1.692 ± 0.065	0.582PheLys: 0.582 ± 0.042	2.885PheLeu: 2.885 ± 0.092	0.553PheMet: 0.553 ± 0.032	0.859PheAsn: 0.859 ± 0.046	1.599PhePro: 1.599 ± 0.052	0.91PheGln: 0.91 ± 0.041	1.915PheArg: 1.915 ± 0.065	1.752PheSer: 1.752 ± 0.062	2.336PheThr: 2.336 ± 0.086	3.201PheVal: 3.201 ± 0.088	0.464PheTrp: 0.464 ± 0.031	0.745PheTyr: 0.745 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	9.512GlyAla: 9.512 ± 0.154	0.551GlyCys: 0.551 ± 0.036	4.98GlyAsp: 4.98 ± 0.107	4.623GlyGlu: 4.623 ± 0.114	3.428GlyPhe: 3.428 ± 0.093	6.74GlyGly: 6.74 ± 0.149	1.878GlyHis: 1.878 ± 0.06	5.791GlyIle: 5.791 ± 0.12	2.563GlyLys: 2.563 ± 0.081	7.935GlyLeu: 7.935 ± 0.144	1.981GlyMet: 1.981 ± 0.063	2.119GlyAsn: 2.119 ± 0.073	2.947GlyPro: 2.947 ± 0.069	2.398GlyGln: 2.398 ± 0.073	4.943GlyArg: 4.943 ± 0.124	5.081GlySer: 5.081 ± 0.129	5.477GlyThr: 5.477 ± 0.131	7.632GlyVal: 7.632 ± 0.124	1.354GlyTrp: 1.354 ± 0.068	2.198GlyTyr: 2.198 ± 0.075	0.0GlyXaa: 0.0 ± 0.0
His	2.34HisAla: 2.34 ± 0.07	0.144HisCys: 0.144 ± 0.018	1.482HisAsp: 1.482 ± 0.052	1.362HisGlu: 1.362 ± 0.053	0.617HisPhe: 0.617 ± 0.037	1.93HisGly: 1.93 ± 0.061	0.66HisHis: 0.66 ± 0.042	1.236HisIle: 1.236 ± 0.051	0.458HisLys: 0.458 ± 0.034	1.909HisLeu: 1.909 ± 0.062	0.423HisMet: 0.423 ± 0.031	0.623HisAsn: 0.623 ± 0.037	1.387HisPro: 1.387 ± 0.061	0.539HisGln: 0.539 ± 0.033	1.568HisArg: 1.568 ± 0.061	1.238HisSer: 1.238 ± 0.055	1.234HisThr: 1.234 ± 0.053	1.793HisVal: 1.793 ± 0.06	0.215HisTrp: 0.215 ± 0.021	0.477HisTyr: 0.477 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	8.088IleAla: 8.088 ± 0.139	0.27IleCys: 0.27 ± 0.023	4.099IleAsp: 4.099 ± 0.103	3.669IleGlu: 3.669 ± 0.102	1.63IlePhe: 1.63 ± 0.066	4.743IleGly: 4.743 ± 0.116	1.141IleHis: 1.141 ± 0.045	2.749IleIle: 2.749 ± 0.076	1.166IleLys: 1.166 ± 0.055	4.272IleLeu: 4.272 ± 0.104	0.873IleMet: 0.873 ± 0.045	1.41IleAsn: 1.41 ± 0.053	2.957IlePro: 2.957 ± 0.08	1.265IleGln: 1.265 ± 0.045	3.473IleArg: 3.473 ± 0.087	3.032IleSer: 3.032 ± 0.078	3.799IleThr: 3.799 ± 0.098	5.859IleVal: 5.859 ± 0.132	0.526IleTrp: 0.526 ± 0.035	0.933IleTyr: 0.933 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	2.881LysAla: 2.881 ± 0.09	0.093LysCys: 0.093 ± 0.014	1.37LysAsp: 1.37 ± 0.057	1.127LysGlu: 1.127 ± 0.063	0.867LysPhe: 0.867 ± 0.041	1.878LysGly: 1.878 ± 0.078	0.572LysHis: 0.572 ± 0.04	1.362LysIle: 1.362 ± 0.061	1.061LysLys: 1.061 ± 0.066	2.208LysLeu: 2.208 ± 0.071	0.59LysMet: 0.59 ± 0.036	0.774LysAsn: 0.774 ± 0.045	1.498LysPro: 1.498 ± 0.06	0.747LysGln: 0.747 ± 0.043	1.791LysArg: 1.791 ± 0.071	1.583LysSer: 1.583 ± 0.064	1.55LysThr: 1.55 ± 0.068	2.029LysVal: 2.029 ± 0.071	0.336LysTrp: 0.336 ± 0.029	0.617LysTyr: 0.617 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	12.457LeuAla: 12.457 ± 0.168	0.57LeuCys: 0.57 ± 0.036	6.212LeuAsp: 6.212 ± 0.113	5.143LeuGlu: 5.143 ± 0.127	2.943LeuPhe: 2.943 ± 0.089	8.276LeuGly: 8.276 ± 0.146	1.781LeuHis: 1.781 ± 0.075	4.701LeuIle: 4.701 ± 0.118	2.113LeuLys: 2.113 ± 0.09	7.896LeuLeu: 7.896 ± 0.164	1.847LeuMet: 1.847 ± 0.071	2.396LeuAsn: 2.396 ± 0.071	4.695LeuPro: 4.695 ± 0.1	2.161LeuGln: 2.161 ± 0.078	6.032LeuArg: 6.032 ± 0.126	5.871LeuSer: 5.871 ± 0.109	5.929LeuThr: 5.929 ± 0.109	8.994LeuVal: 8.994 ± 0.138	1.261LeuTrp: 1.261 ± 0.054	1.723LeuTyr: 1.723 ± 0.073	0.0LeuXaa: 0.0 ± 0.0
Met	2.493MetAla: 2.493 ± 0.072	0.126MetCys: 0.126 ± 0.016	1.067MetAsp: 1.067 ± 0.051	0.881MetGlu: 0.881 ± 0.045	0.702MetPhe: 0.702 ± 0.04	1.698MetGly: 1.698 ± 0.07	0.376MetHis: 0.376 ± 0.03	1.026MetIle: 1.026 ± 0.049	0.642MetLys: 0.642 ± 0.038	1.729MetLeu: 1.729 ± 0.063	0.384MetMet: 0.384 ± 0.028	0.757MetAsn: 0.757 ± 0.039	1.174MetPro: 1.174 ± 0.051	0.481MetGln: 0.481 ± 0.031	1.401MetArg: 1.401 ± 0.056	1.822MetSer: 1.822 ± 0.059	1.855MetThr: 1.855 ± 0.055	1.75MetVal: 1.75 ± 0.059	0.289MetTrp: 0.289 ± 0.024	0.427MetTyr: 0.427 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.1AsnAla: 3.1 ± 0.09	0.163AsnCys: 0.163 ± 0.02	1.694AsnAsp: 1.694 ± 0.059	1.346AsnGlu: 1.346 ± 0.048	0.923AsnPhe: 0.923 ± 0.043	2.545AsnGly: 2.545 ± 0.087	0.551AsnHis: 0.551 ± 0.033	1.37AsnIle: 1.37 ± 0.057	0.652AsnLys: 0.652 ± 0.039	2.654AsnLeu: 2.654 ± 0.072	0.528AsnMet: 0.528 ± 0.031	0.733AsnAsn: 0.733 ± 0.041	2.053AsnPro: 2.053 ± 0.055	0.706AsnGln: 0.706 ± 0.037	1.597AsnArg: 1.597 ± 0.06	1.571AsnSer: 1.571 ± 0.063	1.552AsnThr: 1.552 ± 0.058	2.041AsnVal: 2.041 ± 0.068	0.413AsnTrp: 0.413 ± 0.032	0.609AsnTyr: 0.609 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	5.995ProAla: 5.995 ± 0.123	0.182ProCys: 0.182 ± 0.02	3.003ProAsp: 3.003 ± 0.077	3.519ProGlu: 3.519 ± 0.091	1.686ProPhe: 1.686 ± 0.058	3.981ProGly: 3.981 ± 0.097	1.18ProHis: 1.18 ± 0.056	2.386ProIle: 2.386 ± 0.072	1.187ProLys: 1.187 ± 0.054	4.359ProLeu: 4.359 ± 0.11	0.918ProMet: 0.918 ± 0.042	1.29ProAsn: 1.29 ± 0.049	1.374ProPro: 1.374 ± 0.059	1.261ProGln: 1.261 ± 0.056	2.809ProArg: 2.809 ± 0.079	2.697ProSer: 2.697 ± 0.085	3.314ProThr: 3.314 ± 0.091	4.367ProVal: 4.367 ± 0.112	0.731ProTrp: 0.731 ± 0.042	1.055ProTyr: 1.055 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	2.992GlnAla: 2.992 ± 0.079	0.151GlnCys: 0.151 ± 0.019	1.147GlnAsp: 1.147 ± 0.054	1.081GlnGlu: 1.081 ± 0.052	1.032GlnPhe: 1.032 ± 0.045	1.919GlnGly: 1.919 ± 0.064	0.642GlnHis: 0.642 ± 0.037	1.668GlnIle: 1.668 ± 0.06	0.836GlnLys: 0.836 ± 0.052	2.679GlnLeu: 2.679 ± 0.079	0.648GlnMet: 0.648 ± 0.038	0.749GlnAsn: 0.749 ± 0.039	1.315GlnPro: 1.315 ± 0.053	0.904GlnGln: 0.904 ± 0.049	2.14GlnArg: 2.14 ± 0.079	1.597GlnSer: 1.597 ± 0.056	1.498GlnThr: 1.498 ± 0.059	2.254GlnVal: 2.254 ± 0.069	0.524GlnTrp: 0.524 ± 0.046	0.629GlnTyr: 0.629 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	7.522ArgAla: 7.522 ± 0.138	0.308ArgCys: 0.308 ± 0.026	4.28ArgAsp: 4.28 ± 0.098	4.123ArgGlu: 4.123 ± 0.112	2.332ArgPhe: 2.332 ± 0.079	4.957ArgGly: 4.957 ± 0.122	1.54ArgHis: 1.54 ± 0.049	3.876ArgIle: 3.876 ± 0.085	1.593ArgLys: 1.593 ± 0.065	5.853ArgLeu: 5.853 ± 0.119	1.513ArgMet: 1.513 ± 0.054	1.713ArgAsn: 1.713 ± 0.055	2.737ArgPro: 2.737 ± 0.133	1.762ArgGln: 1.762 ± 0.066	4.842ArgArg: 4.842 ± 0.124	3.55ArgSer: 3.55 ± 0.084	4.035ArgThr: 4.035 ± 0.095	5.636ArgVal: 5.636 ± 0.112	1.04ArgTrp: 1.04 ± 0.048	1.476ArgTyr: 1.476 ± 0.062	0.0ArgXaa: 0.0 ± 0.0
Ser	7.403SerAla: 7.403 ± 0.128	0.378SerCys: 0.378 ± 0.032	3.581SerAsp: 3.581 ± 0.084	3.164SerGlu: 3.164 ± 0.094	2.08SerPhe: 2.08 ± 0.07	5.646SerGly: 5.646 ± 0.129	1.234SerHis: 1.234 ± 0.049	3.267SerIle: 3.267 ± 0.088	1.292SerLys: 1.292 ± 0.05	5.607SerLeu: 5.607 ± 0.104	1.447SerMet: 1.447 ± 0.053	1.604SerAsn: 1.604 ± 0.064	2.862SerPro: 2.862 ± 0.078	1.56SerGln: 1.56 ± 0.058	3.808SerArg: 3.808 ± 0.089	3.349SerSer: 3.349 ± 0.092	4.0SerThr: 4.0 ± 0.103	5.316SerVal: 5.316 ± 0.121	0.828SerTrp: 0.828 ± 0.038	1.35SerTyr: 1.35 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	7.506ThrAla: 7.506 ± 0.135	0.332ThrCys: 0.332 ± 0.033	3.962ThrAsp: 3.962 ± 0.09	3.655ThrGlu: 3.655 ± 0.085	2.155ThrPhe: 2.155 ± 0.078	5.653ThrGly: 5.653 ± 0.115	1.377ThrHis: 1.377 ± 0.055	3.713ThrIle: 3.713 ± 0.086	1.529ThrLys: 1.529 ± 0.057	6.032ThrLeu: 6.032 ± 0.119	1.259ThrMet: 1.259 ± 0.051	1.75ThrAsn: 1.75 ± 0.057	3.552ThrPro: 3.552 ± 0.084	1.517ThrGln: 1.517 ± 0.065	3.595ThrArg: 3.595 ± 0.1	3.44ThrSer: 3.44 ± 0.091	4.142ThrThr: 4.142 ± 0.125	6.542ThrVal: 6.542 ± 0.122	0.813ThrTrp: 0.813 ± 0.041	1.478ThrTyr: 1.478 ± 0.073	0.0ThrXaa: 0.0 ± 0.0
Val	11.623ValAla: 11.623 ± 0.174	0.551ValCys: 0.551 ± 0.032	5.706ValAsp: 5.706 ± 0.115	4.891ValGlu: 4.891 ± 0.11	2.972ValPhe: 2.972 ± 0.08	7.12ValGly: 7.12 ± 0.121	1.781ValHis: 1.781 ± 0.072	5.176ValIle: 5.176 ± 0.092	2.078ValLys: 2.078 ± 0.08	8.243ValLeu: 8.243 ± 0.155	1.855ValMet: 1.855 ± 0.066	2.454ValAsn: 2.454 ± 0.074	4.367ValPro: 4.367 ± 0.083	2.051ValGln: 2.051 ± 0.061	5.488ValArg: 5.488 ± 0.111	5.909ValSer: 5.909 ± 0.133	6.424ValThr: 6.424 ± 0.128	9.223ValVal: 9.223 ± 0.19	1.255ValTrp: 1.255 ± 0.049	1.61ValTyr: 1.61 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	1.571TrpAla: 1.571 ± 0.068	0.101TrpCys: 0.101 ± 0.016	0.792TrpAsp: 0.792 ± 0.046	0.623TrpGlu: 0.623 ± 0.034	0.598TrpPhe: 0.598 ± 0.045	1.189TrpGly: 1.189 ± 0.055	0.308TrpHis: 0.308 ± 0.027	0.731TrpIle: 0.731 ± 0.037	0.367TrpLys: 0.367 ± 0.03	1.486TrpLeu: 1.486 ± 0.073	0.376TrpMet: 0.376 ± 0.028	0.518TrpAsn: 0.518 ± 0.038	0.584TrpPro: 0.584 ± 0.036	0.44TrpGln: 0.44 ± 0.028	0.982TrpArg: 0.982 ± 0.043	0.98TrpSer: 0.98 ± 0.045	0.817TrpThr: 0.817 ± 0.049	1.143TrpVal: 1.143 ± 0.052	0.347TrpTrp: 0.347 ± 0.029	0.295TrpTyr: 0.295 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.421TyrAla: 2.421 ± 0.078	0.138TyrCys: 0.138 ± 0.016	1.474TyrAsp: 1.474 ± 0.057	1.156TyrGlu: 1.156 ± 0.055	0.889TyrPhe: 0.889 ± 0.048	2.01TyrGly: 2.01 ± 0.066	0.341TyrHis: 0.341 ± 0.028	0.914TyrIle: 0.914 ± 0.04	0.475TyrLys: 0.475 ± 0.036	2.159TyrLeu: 2.159 ± 0.069	0.351TyrMet: 0.351 ± 0.029	0.547TyrAsn: 0.547 ± 0.034	1.053TyrPro: 1.053 ± 0.049	0.611TyrGln: 0.611 ± 0.039	1.589TyrArg: 1.589 ± 0.062	1.467TyrSer: 1.467 ± 0.056	1.321TyrThr: 1.321 ± 0.067	1.744TyrVal: 1.744 ± 0.055	0.316TyrTrp: 0.316 ± 0.024	0.506TyrTyr: 0.506 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1490 proteins (484553 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---