Cafeteria roenbergensis (Marine flagellate)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Bigyra; Opalozoa; Bicosoecida; Cafeteriaceae; Cafeteria

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8530 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A8CVE5|A0A5A8CVE5_CAFRO Uncharacterized protein OS=Cafeteria roenbergensis OX=33653 GN=FNF29_01199 PE=4 SV=1
MM1 pKa = 7.38NAFDD5 pKa = 6.33GPIHH9 pKa = 5.94WQQIATSGAYY19 pKa = 10.1VFVTILGLVTIPHH32 pKa = 6.23MFSNSEE38 pKa = 4.0LPLALSLTIYY48 pKa = 10.79LLLAAVCVASWLYY61 pKa = 10.49IEE63 pKa = 5.09LTDD66 pKa = 3.79PSMPGGVACPCMKK79 pKa = 9.72RR80 pKa = 11.84TQSSQSNCSLCRR92 pKa = 11.84KK93 pKa = 8.95PVVGLDD99 pKa = 2.98HH100 pKa = 7.13HH101 pKa = 7.24CYY103 pKa = 10.19FLNTCIGKK111 pKa = 9.92RR112 pKa = 11.84NYY114 pKa = 10.88AHH116 pKa = 7.0FFALSLCSTLQMAAQVVFSVLGMTVWIEE144 pKa = 3.88RR145 pKa = 11.84GNDD148 pKa = 2.9NVLYY152 pKa = 9.65YY153 pKa = 10.96VLVGLQALIAAVAGGATCSLLGFHH177 pKa = 7.22VYY179 pKa = 10.53LLWIGMGTYY188 pKa = 10.68DD189 pKa = 5.07WMWAKK194 pKa = 10.03QDD196 pKa = 3.33KK197 pKa = 10.26AAEE200 pKa = 4.15KK201 pKa = 10.53VRR203 pKa = 11.84RR204 pKa = 11.84EE205 pKa = 3.69NQARR209 pKa = 11.84FEE211 pKa = 4.43AEE213 pKa = 3.67QQARR217 pKa = 11.84LQLEE221 pKa = 4.31RR222 pKa = 11.84EE223 pKa = 4.17RR224 pKa = 11.84QQAHH228 pKa = 5.9GAAPAMPAGRR238 pKa = 11.84VAVTVEE244 pKa = 3.97SHH246 pKa = 6.38PVAAAAGAAAAAGVGGDD263 pKa = 3.81SGGGLYY269 pKa = 10.52AGFEE273 pKa = 4.28PEE275 pKa = 3.76TDD277 pKa = 3.48EE278 pKa = 5.48EE279 pKa = 4.52EE280 pKa = 4.7GGTAGGADD288 pKa = 3.77TASSPGDD295 pKa = 3.43DD296 pKa = 3.91YY297 pKa = 12.1GGVDD301 pKa = 3.81AGEE304 pKa = 4.64GGAGAEE310 pKa = 4.21SQDD313 pKa = 4.05VIGMEE318 pKa = 4.28YY319 pKa = 10.52ADD321 pKa = 4.19EE322 pKa = 4.8AGGVYY327 pKa = 10.56DD328 pKa = 5.65DD329 pKa = 5.35DD330 pKa = 4.83AAGAYY335 pKa = 10.27DD336 pKa = 4.76DD337 pKa = 5.41DD338 pKa = 4.69AAGGAFDD345 pKa = 5.09DD346 pKa = 4.81EE347 pKa = 4.52GRR349 pKa = 11.84EE350 pKa = 3.94GDD352 pKa = 3.78GFGDD356 pKa = 3.85EE357 pKa = 4.53EE358 pKa = 5.67DD359 pKa = 4.1EE360 pKa = 4.5ADD362 pKa = 3.46HH363 pKa = 6.82EE364 pKa = 4.5RR365 pKa = 11.84VQRR368 pKa = 11.84VPGASSAGSTGAPSGDD384 pKa = 3.82SAPGSPTQAPTEE396 pKa = 4.09EE397 pKa = 4.69GGAEE401 pKa = 3.74QGAEE405 pKa = 4.06AEE407 pKa = 4.55EE408 pKa = 4.52AGSPSDD414 pKa = 4.28AGGEE418 pKa = 3.93AHH420 pKa = 6.1GQEE423 pKa = 4.3GAEE426 pKa = 3.97QEE428 pKa = 4.37EE429 pKa = 4.81AEE431 pKa = 4.43DD432 pKa = 3.89AASGEE437 pKa = 4.38AASGSQAAGEE447 pKa = 4.06PAEE450 pKa = 4.34TEE452 pKa = 3.99ATAEE456 pKa = 4.08ATGPEE461 pKa = 3.97AAAIAQEE468 pKa = 4.27EE469 pKa = 4.39SDD471 pKa = 5.02ADD473 pKa = 3.7AADD476 pKa = 4.22AGDD479 pKa = 4.8DD480 pKa = 3.75AVADD484 pKa = 4.39AGDD487 pKa = 4.04DD488 pKa = 3.71AGDD491 pKa = 3.92AAVDD495 pKa = 4.26DD496 pKa = 5.16AGADD500 pKa = 3.48AGDD503 pKa = 4.04DD504 pKa = 3.23AGAPGEE510 pKa = 4.31EE511 pKa = 4.43EE512 pKa = 4.18GLADD516 pKa = 4.6GAADD520 pKa = 3.72AAAPAAPVAAPEE532 pKa = 4.62GGPEE536 pKa = 4.25GDD538 pKa = 4.05SCDD541 pKa = 3.48STGRR545 pKa = 11.84PGSAGEE551 pKa = 4.27LQPEE555 pKa = 4.49TATPRR560 pKa = 11.84PGEE563 pKa = 3.9AA564 pKa = 3.76

Molecular weight: 56.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A8CW52|A0A5A8CW52_CAFRO Uncharacterized protein OS=Cafeteria roenbergensis OX=33653 GN=FNF29_00708 PE=4 SV=1
MM1 pKa = 7.51AALGVVVFFIVLVLPMLAITFDD23 pKa = 3.36AARR26 pKa = 11.84RR27 pKa = 11.84NSPRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84LDD35 pKa = 3.33PGTTLVVLMVLSMTPHH51 pKa = 6.33AMGLALRR58 pKa = 11.84EE59 pKa = 4.16ALLATARR66 pKa = 11.84GSLIRR71 pKa = 11.84LVVTQATVLFCTALTWVMEE90 pKa = 4.42LAAQAMTPIEE100 pKa = 4.56AEE102 pKa = 4.04RR103 pKa = 11.84QPLLVAADD111 pKa = 3.52VMLTVFLNLVTAQEE125 pKa = 4.24LAVGTVEE132 pKa = 4.81FWLTLAVRR140 pKa = 11.84CAGLLFMDD148 pKa = 3.62TRR150 pKa = 11.84LWEE153 pKa = 4.41DD154 pKa = 3.5VQSLFTNKK162 pKa = 9.29RR163 pKa = 11.84RR164 pKa = 11.84WFRR167 pKa = 11.84FVTARR172 pKa = 11.84LGWLAVQRR180 pKa = 11.84ADD182 pKa = 3.22RR183 pKa = 11.84VLVSEE188 pKa = 4.06QLALVISAAYY198 pKa = 8.28LTGGAAGALAGFVSAQEE215 pKa = 3.8ATAAEE220 pKa = 4.32NPRR223 pKa = 11.84EE224 pKa = 4.07VVGRR228 pKa = 11.84VTAFAVIFVGISIMVPVVRR247 pKa = 11.84YY248 pKa = 7.69LTYY251 pKa = 11.15AKK253 pKa = 10.33LARR256 pKa = 11.84ARR258 pKa = 11.84LKK260 pKa = 10.62SAAVSILGSCTVLNLVKK277 pKa = 10.36DD278 pKa = 3.53RR279 pKa = 11.84HH280 pKa = 5.27AAHH283 pKa = 7.53RR284 pKa = 11.84IAGVAAAGGSFFGDD298 pKa = 3.35DD299 pKa = 3.53CKK301 pKa = 10.85RR302 pKa = 11.84WGVASRR308 pKa = 11.84EE309 pKa = 4.33SLSDD313 pKa = 3.4RR314 pKa = 11.84SPPFASVRR322 pKa = 11.84QHH324 pKa = 6.25RR325 pKa = 11.84GSFVPYY331 pKa = 10.27VFKK334 pKa = 10.75EE335 pKa = 3.88WVNGVRR341 pKa = 11.84QSRR344 pKa = 11.84GKK346 pKa = 9.07GCARR350 pKa = 11.84PRR352 pKa = 11.84CIRR355 pKa = 11.84VGILCFGDD363 pKa = 3.44ARR365 pKa = 11.84SSGQHH370 pKa = 3.83WVAFQTEE377 pKa = 4.25YY378 pKa = 11.54AFAVMLFAALWGSWASSVALGVKK401 pKa = 10.09AFSS404 pKa = 3.5

Molecular weight: 43.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8424

106

8530

5899624

66

17662

691.6

72.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

17.239 ± 0.069

1.257 ± 0.01

5.696 ± 0.022

5.814 ± 0.031

2.71 ± 0.017

9.457 ± 0.036

1.863 ± 0.012

2.439 ± 0.019

2.589 ± 0.023

8.728 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.896 ± 0.012

1.613 ± 0.015

5.825 ± 0.026

2.824 ± 0.012

7.383 ± 0.03

8.051 ± 0.029

4.787 ± 0.027

7.265 ± 0.035

1.271 ± 0.009

1.293 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski