Enterobacter phage E-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus; Enterobacter virus E3

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3M2Y5|A0A0E3M2Y5_9CAUD Terminase large subunit OS=Enterobacter phage E-3 OX=1636314 PE=3 SV=1
MM1 pKa = 7.73IMPKK5 pKa = 10.08SDD7 pKa = 3.6TVTMTRR13 pKa = 11.84DD14 pKa = 2.64AWNDD18 pKa = 2.85VSAYY22 pKa = 9.84IEE24 pKa = 4.04FLEE27 pKa = 5.11GRR29 pKa = 11.84LAKK32 pKa = 10.65LNALEE37 pKa = 4.13AHH39 pKa = 6.92GVDD42 pKa = 3.42NWCGYY47 pKa = 10.92DD48 pKa = 3.57DD49 pKa = 5.3AVAEE53 pKa = 4.29LFKK56 pKa = 10.99EE57 pKa = 4.52DD58 pKa = 3.46EE59 pKa = 4.27

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3M4J0|A0A0E3M4J0_9CAUD Uncharacterized protein OS=Enterobacter phage E-3 OX=1636314 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84LHH4 pKa = 6.7FNKK7 pKa = 10.46SNGIFSVRR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 10.21DD18 pKa = 3.31RR19 pKa = 11.84STVLASEE26 pKa = 4.67RR27 pKa = 11.84NAKK30 pKa = 10.2LPLIGSVATLSPCVHH45 pKa = 6.91LLITHH50 pKa = 6.15GEE52 pKa = 4.09FVKK55 pKa = 10.87AMNKK59 pKa = 8.58EE60 pKa = 4.14RR61 pKa = 11.84PHH63 pKa = 8.07LEE65 pKa = 3.6AVVTYY70 pKa = 8.64WPRR73 pKa = 11.84IRR75 pKa = 11.84LFFKK79 pKa = 9.96WIKK82 pKa = 10.16EE83 pKa = 4.1VLL85 pKa = 3.56

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

9411

37

1320

261.4

29.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.404 ± 0.487

0.733 ± 0.19

6.535 ± 0.226

7.172 ± 0.465

3.592 ± 0.244

7.853 ± 0.392

1.721 ± 0.243

5.143 ± 0.242

6.45 ± 0.498

7.959 ± 0.41

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.89 ± 0.256

4.654 ± 0.469

3.581 ± 0.253

4.059 ± 0.439

5.749 ± 0.216

6.035 ± 0.39

5.334 ± 0.288

6.514 ± 0.415

1.413 ± 0.206

3.209 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski