Porcine rotavirus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q06B31|Q06B31_9REOV Non-structural protein 5 OS=Porcine rotavirus A OX=10967 GN=NSP5 PE=3 SV=1
MM1 pKa = 7.26YY2 pKa = 10.2GIEE5 pKa = 4.22YY6 pKa = 7.59TTILTFLISLIFINYY21 pKa = 8.0ILKK24 pKa = 10.48SVTRR28 pKa = 11.84TMDD31 pKa = 3.3FVIYY35 pKa = 9.85RR36 pKa = 11.84SLFIIVMLAPFIKK49 pKa = 8.7TQNYY53 pKa = 9.32GINLPITGSMDD64 pKa = 3.49TPYY67 pKa = 10.26MNSTASEE74 pKa = 4.34TFLTSTLCLYY84 pKa = 10.23YY85 pKa = 10.01PNEE88 pKa = 4.23AARR91 pKa = 11.84QIADD95 pKa = 4.6DD96 pKa = 3.6KK97 pKa = 11.4WKK99 pKa = 9.31DD100 pKa = 3.44TLSQLFLTKK109 pKa = 10.05GWPTGSVYY117 pKa = 10.44FKK119 pKa = 10.13EE120 pKa = 4.36YY121 pKa = 10.91VDD123 pKa = 3.34IASFSVDD130 pKa = 3.05PQLYY134 pKa = 8.88CDD136 pKa = 3.82YY137 pKa = 10.47NIVIMKK143 pKa = 10.15YY144 pKa = 10.48DD145 pKa = 3.49VDD147 pKa = 4.15SQLDD151 pKa = 3.66MSEE154 pKa = 4.1LADD157 pKa = 5.13LILNEE162 pKa = 4.24WLCNPMDD169 pKa = 3.6IALYY173 pKa = 10.23YY174 pKa = 9.03YY175 pKa = 10.41QQTDD179 pKa = 3.57EE180 pKa = 4.17ANKK183 pKa = 8.13WISMGTSCTIKK194 pKa = 10.29VCPLNTQTLGVGCLTTDD211 pKa = 3.05PTTFEE216 pKa = 3.89EE217 pKa = 4.17VAYY220 pKa = 10.34AEE222 pKa = 4.51KK223 pKa = 10.68LAITDD228 pKa = 3.46VVDD231 pKa = 4.79GVDD234 pKa = 3.32HH235 pKa = 6.79KK236 pKa = 11.72LDD238 pKa = 3.66VTTTTCTIRR247 pKa = 11.84NCKK250 pKa = 9.83KK251 pKa = 10.18LGPRR255 pKa = 11.84EE256 pKa = 3.99NVAVIQIGGSNILDD270 pKa = 3.33ITADD274 pKa = 3.68PTTAPQTEE282 pKa = 3.66RR283 pKa = 11.84MMRR286 pKa = 11.84INWKK290 pKa = 9.54KK291 pKa = 7.26WWQVFYY297 pKa = 10.66TIVDD301 pKa = 3.57YY302 pKa = 11.59VNQIVQAMSKK312 pKa = 10.16RR313 pKa = 11.84SRR315 pKa = 11.84SS316 pKa = 3.46

Molecular weight: 36.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3Q266|A0A0D3Q266_9REOV Non-structural protein 3 OS=Porcine rotavirus A OX=10967 GN=NSP3 PE=3 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.64LACFCYY9 pKa = 9.75PHH11 pKa = 7.6LEE13 pKa = 3.86NDD15 pKa = 3.51SYY17 pKa = 11.97KK18 pKa = 10.64FIPFNSLAIKK28 pKa = 10.62CMLTAKK34 pKa = 9.91VDD36 pKa = 3.91KK37 pKa = 10.63KK38 pKa = 11.12DD39 pKa = 3.04QDD41 pKa = 3.14KK42 pKa = 10.51FYY44 pKa = 11.42NSIIYY49 pKa = 9.99GIAPPPQFKK58 pKa = 10.27KK59 pKa = 10.52RR60 pKa = 11.84YY61 pKa = 5.79NTSDD65 pKa = 2.79NSRR68 pKa = 11.84GMNYY72 pKa = 7.22EE73 pKa = 3.56TTMFNKK79 pKa = 9.56VAALVCEE86 pKa = 4.32ALNSVKK92 pKa = 9.08ITQSDD97 pKa = 4.0LANVLSRR104 pKa = 11.84VVSVRR109 pKa = 11.84HH110 pKa = 5.93LEE112 pKa = 3.89NLVLRR117 pKa = 11.84KK118 pKa = 9.59EE119 pKa = 4.02NHH121 pKa = 5.63QDD123 pKa = 3.26VLFHH127 pKa = 6.93SKK129 pKa = 10.35EE130 pKa = 3.82LLLRR134 pKa = 11.84SVLIAIGQIKK144 pKa = 9.9EE145 pKa = 3.96IEE147 pKa = 4.25TTATAEE153 pKa = 3.87GGEE156 pKa = 4.08IVFQNAAFTMWKK168 pKa = 8.49LTYY171 pKa = 9.82LDD173 pKa = 4.31HH174 pKa = 8.04KK175 pKa = 10.88LMPILDD181 pKa = 3.78QNFIEE186 pKa = 4.65YY187 pKa = 10.13KK188 pKa = 9.01ITVNEE193 pKa = 4.63DD194 pKa = 2.89KK195 pKa = 10.59PISDD199 pKa = 3.42VHH201 pKa = 7.37IKK203 pKa = 10.54EE204 pKa = 4.36LVSEE208 pKa = 5.08LRR210 pKa = 11.84WQYY213 pKa = 11.88NKK215 pKa = 10.27FAVITHH221 pKa = 6.31GKK223 pKa = 7.43GHH225 pKa = 5.73YY226 pKa = 9.48RR227 pKa = 11.84VVKK230 pKa = 8.49YY231 pKa = 10.96SSVANHH237 pKa = 7.18ADD239 pKa = 3.3RR240 pKa = 11.84VFATFKK246 pKa = 11.24NNAKK250 pKa = 10.09SGNITEE256 pKa = 5.07FNLLDD261 pKa = 5.23QRR263 pKa = 11.84IIWQNWYY270 pKa = 10.72AFTSSMKK277 pKa = 10.28QGNTLDD283 pKa = 3.36VCKK286 pKa = 10.46RR287 pKa = 11.84LLFQKK292 pKa = 9.69MKK294 pKa = 10.49QEE296 pKa = 4.09KK297 pKa = 10.31NPFKK301 pKa = 10.91GLSTDD306 pKa = 2.99RR307 pKa = 11.84KK308 pKa = 8.31MDD310 pKa = 3.55EE311 pKa = 4.04VSQIGII317 pKa = 3.75

Molecular weight: 36.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

3797

175

517

345.2

39.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.083 ± 0.379

1.422 ± 0.359

6.321 ± 0.439

5.952 ± 0.572

4.135 ± 0.33

3.081 ± 0.267

1.844 ± 0.331

7.453 ± 0.315

6.769 ± 0.606

9.033 ± 0.383

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.213 ± 0.288

6.821 ± 0.473

3.424 ± 0.317

4.214 ± 0.433

4.767 ± 0.326

7.585 ± 0.798

6.61 ± 0.633

6.11 ± 0.272

1.317 ± 0.17

4.846 ± 0.483
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski