Xanthomonadaceae bacterium NML91-0213

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; unclassified Xanthomonadaceae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2879 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9E6K5|A0A2M9E6K5_9GAMM AAA family ATPase OS=Xanthomonadaceae bacterium NML91-0213 OX=2032582 GN=CO641_07920 PE=4 SV=1
MM1 pKa = 7.18NPKK4 pKa = 8.78LTTLSLTCALALAACGGDD22 pKa = 3.94GADD25 pKa = 4.28QQDD28 pKa = 3.62TTAATTPAPATAPATDD44 pKa = 3.7TTNANAGSVPGDD56 pKa = 3.67GMGTDD61 pKa = 3.7AGMATSQDD69 pKa = 3.45PAATAGADD77 pKa = 3.44AAPASNKK84 pKa = 9.84PEE86 pKa = 3.85ATVTNCATTIQGDD99 pKa = 3.68DD100 pKa = 3.19AMQFSVGSITVPSSCSEE117 pKa = 3.69FTITLEE123 pKa = 4.17HH124 pKa = 6.42TGQLPVAAMGHH135 pKa = 5.24NVVVSQASDD144 pKa = 3.07RR145 pKa = 11.84AGIATDD151 pKa = 3.66GMAAGVDD158 pKa = 3.62GGYY161 pKa = 10.1VPQGDD166 pKa = 3.88ARR168 pKa = 11.84VIAATEE174 pKa = 4.48LIGGGQTTSVTFPVSALQGGGPYY197 pKa = 10.54EE198 pKa = 4.37FFCSFPGHH206 pKa = 5.66WAVMRR211 pKa = 11.84GSIQVGG217 pKa = 3.26

Molecular weight: 21.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9E0I2|A0A2M9E0I2_9GAMM RNA polymerase subunit sigma-24 OS=Xanthomonadaceae bacterium NML91-0213 OX=2032582 GN=CO641_14280 PE=3 SV=1
MM1 pKa = 7.05ATKK4 pKa = 10.57KK5 pKa = 9.26ATKK8 pKa = 10.06KK9 pKa = 9.86SATRR13 pKa = 11.84KK14 pKa = 6.08TAKK17 pKa = 9.95KK18 pKa = 9.38AAATKK23 pKa = 10.17RR24 pKa = 11.84VSKK27 pKa = 10.1VAKK30 pKa = 9.81KK31 pKa = 9.19ATARR35 pKa = 11.84KK36 pKa = 8.7AAAKK40 pKa = 8.14KK41 pKa = 6.01TTARR45 pKa = 11.84KK46 pKa = 8.84AAAKK50 pKa = 8.14KK51 pKa = 6.01TTARR55 pKa = 11.84KK56 pKa = 8.84AAAKK60 pKa = 8.08KK61 pKa = 5.95TTARR65 pKa = 11.84KK66 pKa = 9.13SAAKK70 pKa = 8.81KK71 pKa = 5.73TTARR75 pKa = 11.84KK76 pKa = 8.84AAAKK80 pKa = 10.23KK81 pKa = 8.71STARR85 pKa = 11.84KK86 pKa = 8.74AATGRR91 pKa = 11.84KK92 pKa = 7.83STAKK96 pKa = 10.17KK97 pKa = 9.71AATRR101 pKa = 11.84KK102 pKa = 6.18TAKK105 pKa = 10.05KK106 pKa = 10.13AVAKK110 pKa = 10.56RR111 pKa = 11.84PAAKK115 pKa = 10.08KK116 pKa = 8.4ATARR120 pKa = 11.84KK121 pKa = 8.23STAKK125 pKa = 10.26KK126 pKa = 9.52AATRR130 pKa = 11.84KK131 pKa = 7.18TAAKK135 pKa = 8.36KK136 pKa = 7.21TARR139 pKa = 11.84KK140 pKa = 8.22ATAKK144 pKa = 10.18KK145 pKa = 9.49AAPRR149 pKa = 11.84KK150 pKa = 9.37AVAKK154 pKa = 10.22KK155 pKa = 9.02MPRR158 pKa = 11.84RR159 pKa = 11.84AAKK162 pKa = 10.01KK163 pKa = 7.94PAPVAIPAAPTPLMM177 pKa = 4.65

Molecular weight: 18.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2879

0

2879

949773

28

2772

329.9

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.746 ± 0.075

0.795 ± 0.013

6.185 ± 0.037

5.443 ± 0.04

3.21 ± 0.028

8.842 ± 0.045

2.291 ± 0.027

3.979 ± 0.03

1.987 ± 0.037

10.754 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.172 ± 0.021

2.173 ± 0.031

5.502 ± 0.04

3.477 ± 0.029

8.558 ± 0.053

4.766 ± 0.039

4.776 ± 0.033

7.705 ± 0.041

1.524 ± 0.02

2.114 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski