Amino acid dipepetide frequency for Rhodococcus sp. ABRD24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.071AlaAla: 19.071 ± 0.206
0.963AlaCys: 0.963 ± 0.026
8.159AlaAsp: 8.159 ± 0.095
7.915AlaGlu: 7.915 ± 0.083
3.44AlaPhe: 3.44 ± 0.052
11.863AlaGly: 11.863 ± 0.103
2.557AlaHis: 2.557 ± 0.055
5.129AlaIle: 5.129 ± 0.075
2.707AlaLys: 2.707 ± 0.067
13.384AlaLeu: 13.384 ± 0.134
2.639AlaMet: 2.639 ± 0.045
2.312AlaAsn: 2.312 ± 0.04
6.181AlaPro: 6.181 ± 0.097
3.761AlaGln: 3.761 ± 0.051
8.825AlaArg: 8.825 ± 0.1
6.542AlaSer: 6.542 ± 0.076
7.092AlaThr: 7.092 ± 0.078
12.048AlaVal: 12.048 ± 0.124
1.515AlaTrp: 1.515 ± 0.028
2.257AlaTyr: 2.257 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.024CysAla: 1.024 ± 0.03
0.096CysCys: 0.096 ± 0.01
0.497CysAsp: 0.497 ± 0.018
0.422CysGlu: 0.422 ± 0.018
0.258CysPhe: 0.258 ± 0.014
0.893CysGly: 0.893 ± 0.027
0.178CysHis: 0.178 ± 0.01
0.289CysIle: 0.289 ± 0.012
0.129CysLys: 0.129 ± 0.009
0.626CysLeu: 0.626 ± 0.019
0.131CysMet: 0.131 ± 0.009
0.146CysAsn: 0.146 ± 0.011
0.449CysPro: 0.449 ± 0.019
0.182CysGln: 0.182 ± 0.012
0.596CysArg: 0.596 ± 0.024
0.527CysSer: 0.527 ± 0.019
0.498CysThr: 0.498 ± 0.02
0.614CysVal: 0.614 ± 0.022
0.109CysTrp: 0.109 ± 0.009
0.149CysTyr: 0.149 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.938AspAla: 7.938 ± 0.078
0.421AspCys: 0.421 ± 0.017
3.652AspAsp: 3.652 ± 0.05
3.902AspGlu: 3.902 ± 0.054
1.77AspPhe: 1.77 ± 0.038
6.069AspGly: 6.069 ± 0.068
1.345AspHis: 1.345 ± 0.032
2.582AspIle: 2.582 ± 0.048
1.114AspLys: 1.114 ± 0.033
6.407AspLeu: 6.407 ± 0.079
0.914AspMet: 0.914 ± 0.025
1.117AspAsn: 1.117 ± 0.033
4.42AspPro: 4.42 ± 0.063
1.686AspGln: 1.686 ± 0.037
4.962AspArg: 4.962 ± 0.072
3.172AspSer: 3.172 ± 0.055
3.214AspThr: 3.214 ± 0.051
5.227AspVal: 5.227 ± 0.086
0.918AspTrp: 0.918 ± 0.028
1.212AspTyr: 1.212 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
6.191GluAla: 6.191 ± 0.071
0.426GluCys: 0.426 ± 0.018
2.63GluAsp: 2.63 ± 0.043
2.635GluGlu: 2.635 ± 0.049
1.942GluPhe: 1.942 ± 0.037
3.68GluGly: 3.68 ± 0.052
1.533GluHis: 1.533 ± 0.036
2.863GluIle: 2.863 ± 0.045
1.341GluLys: 1.341 ± 0.038
6.908GluLeu: 6.908 ± 0.093
1.1GluMet: 1.1 ± 0.03
1.298GluAsn: 1.298 ± 0.03
2.933GluPro: 2.933 ± 0.046
2.292GluGln: 2.292 ± 0.046
4.85GluArg: 4.85 ± 0.068
3.347GluSer: 3.347 ± 0.046
2.914GluThr: 2.914 ± 0.048
4.602GluVal: 4.602 ± 0.058
0.794GluTrp: 0.794 ± 0.021
1.154GluTyr: 1.154 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.034PheAla: 4.034 ± 0.056
0.28PheCys: 0.28 ± 0.012
2.491PheAsp: 2.491 ± 0.056
1.69PheGlu: 1.69 ± 0.038
0.938PhePhe: 0.938 ± 0.03
3.446PheGly: 3.446 ± 0.057
0.63PheHis: 0.63 ± 0.023
0.938PheIle: 0.938 ± 0.035
0.478PheLys: 0.478 ± 0.021
2.571PheLeu: 2.571 ± 0.059
0.477PheMet: 0.477 ± 0.02
0.606PheAsn: 0.606 ± 0.021
1.399PhePro: 1.399 ± 0.03
0.686PheGln: 0.686 ± 0.024
1.753PheArg: 1.753 ± 0.035
1.684PheSer: 1.684 ± 0.039
2.027PheThr: 2.027 ± 0.044
2.84PheVal: 2.84 ± 0.047
0.375PheTrp: 0.375 ± 0.015
0.621PheTyr: 0.621 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
10.425GlyAla: 10.425 ± 0.095
0.762GlyCys: 0.762 ± 0.025
4.979GlyAsp: 4.979 ± 0.067
4.669GlyGlu: 4.669 ± 0.061
3.066GlyPhe: 3.066 ± 0.053
8.065GlyGly: 8.065 ± 0.1
2.03GlyHis: 2.03 ± 0.04
4.376GlyIle: 4.376 ± 0.054
2.296GlyLys: 2.296 ± 0.046
8.88GlyLeu: 8.88 ± 0.091
2.111GlyMet: 2.111 ± 0.036
1.944GlyAsn: 1.944 ± 0.04
4.5GlyPro: 4.5 ± 0.063
2.561GlyGln: 2.561 ± 0.047
6.704GlyArg: 6.704 ± 0.085
6.021GlySer: 6.021 ± 0.084
5.773GlyThr: 5.773 ± 0.068
7.943GlyVal: 7.943 ± 0.09
1.568GlyTrp: 1.568 ± 0.031
2.249GlyTyr: 2.249 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.392HisAla: 2.392 ± 0.049
0.202HisCys: 0.202 ± 0.011
1.299HisAsp: 1.299 ± 0.032
1.115HisGlu: 1.115 ± 0.034
0.657HisPhe: 0.657 ± 0.022
2.135HisGly: 2.135 ± 0.044
0.682HisHis: 0.682 ± 0.027
0.78HisIle: 0.78 ± 0.025
0.331HisLys: 0.331 ± 0.016
2.119HisLeu: 2.119 ± 0.04
0.36HisMet: 0.36 ± 0.015
0.438HisAsn: 0.438 ± 0.017
1.612HisPro: 1.612 ± 0.037
0.571HisGln: 0.571 ± 0.02
1.847HisArg: 1.847 ± 0.038
1.14HisSer: 1.14 ± 0.029
1.149HisThr: 1.149 ± 0.03
1.712HisVal: 1.712 ± 0.04
0.328HisTrp: 0.328 ± 0.015
0.465HisTyr: 0.465 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.534IleAla: 6.534 ± 0.091
0.354IleCys: 0.354 ± 0.015
3.37IleAsp: 3.37 ± 0.05
2.789IleGlu: 2.789 ± 0.048
0.979IlePhe: 0.979 ± 0.03
4.65IleGly: 4.65 ± 0.059
0.736IleHis: 0.736 ± 0.022
1.302IleIle: 1.302 ± 0.038
0.799IleLys: 0.799 ± 0.028
3.444IleLeu: 3.444 ± 0.058
0.575IleMet: 0.575 ± 0.022
0.88IleAsn: 0.88 ± 0.026
2.377IlePro: 2.377 ± 0.044
0.827IleGln: 0.827 ± 0.024
2.765IleArg: 2.765 ± 0.043
2.346IleSer: 2.346 ± 0.038
2.744IleThr: 2.744 ± 0.045
3.975IleVal: 3.975 ± 0.058
0.389IleTrp: 0.389 ± 0.018
0.753IleTyr: 0.753 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
2.474LysAla: 2.474 ± 0.057
0.123LysCys: 0.123 ± 0.01
1.085LysAsp: 1.085 ± 0.03
0.93LysGlu: 0.93 ± 0.028
0.582LysPhe: 0.582 ± 0.021
1.499LysGly: 1.499 ± 0.039
0.453LysHis: 0.453 ± 0.019
1.051LysIle: 1.051 ± 0.038
0.665LysLys: 0.665 ± 0.031
2.037LysLeu: 2.037 ± 0.039
0.462LysMet: 0.462 ± 0.02
0.498LysAsn: 0.498 ± 0.019
1.222LysPro: 1.222 ± 0.036
0.649LysGln: 0.649 ± 0.023
1.377LysArg: 1.377 ± 0.037
1.262LysSer: 1.262 ± 0.036
1.281LysThr: 1.281 ± 0.042
1.891LysVal: 1.891 ± 0.047
0.302LysTrp: 0.302 ± 0.014
0.471LysTyr: 0.471 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
14.098LeuAla: 14.098 ± 0.137
0.784LeuCys: 0.784 ± 0.025
7.063LeuAsp: 7.063 ± 0.097
5.215LeuGlu: 5.215 ± 0.069
2.773LeuPhe: 2.773 ± 0.039
9.365LeuGly: 9.365 ± 0.112
2.041LeuHis: 2.041 ± 0.044
3.97LeuIle: 3.97 ± 0.057
1.493LeuLys: 1.493 ± 0.041
9.813LeuLeu: 9.813 ± 0.111
1.678LeuMet: 1.678 ± 0.041
1.778LeuAsn: 1.778 ± 0.039
5.803LeuPro: 5.803 ± 0.073
2.366LeuGln: 2.366 ± 0.034
7.721LeuArg: 7.721 ± 0.088
5.962LeuSer: 5.962 ± 0.069
6.522LeuThr: 6.522 ± 0.073
8.955LeuVal: 8.955 ± 0.096
1.215LeuTrp: 1.215 ± 0.033
1.637LeuTyr: 1.637 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.323MetAla: 2.323 ± 0.039
0.187MetCys: 0.187 ± 0.012
0.907MetAsp: 0.907 ± 0.025
0.7MetGlu: 0.7 ± 0.024
0.631MetPhe: 0.631 ± 0.024
1.466MetGly: 1.466 ± 0.035
0.398MetHis: 0.398 ± 0.014
0.895MetIle: 0.895 ± 0.023
0.438MetLys: 0.438 ± 0.02
2.095MetLeu: 2.095 ± 0.036
0.391MetMet: 0.391 ± 0.02
0.495MetAsn: 0.495 ± 0.018
1.192MetPro: 1.192 ± 0.031
0.539MetGln: 0.539 ± 0.02
1.444MetArg: 1.444 ± 0.032
1.616MetSer: 1.616 ± 0.038
1.76MetThr: 1.76 ± 0.033
1.611MetVal: 1.611 ± 0.036
0.282MetTrp: 0.282 ± 0.013
0.343MetTyr: 0.343 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.472AsnAla: 2.472 ± 0.044
0.186AsnCys: 0.186 ± 0.011
1.026AsnAsp: 1.026 ± 0.026
0.967AsnGlu: 0.967 ± 0.029
0.631AsnPhe: 0.631 ± 0.022
1.916AsnGly: 1.916 ± 0.039
0.418AsnHis: 0.418 ± 0.018
0.872AsnIle: 0.872 ± 0.026
0.45AsnLys: 0.45 ± 0.022
2.048AsnLeu: 2.048 ± 0.044
0.403AsnMet: 0.403 ± 0.016
0.476AsnAsn: 0.476 ± 0.021
1.62AsnPro: 1.62 ± 0.034
0.56AsnGln: 0.56 ± 0.02
1.437AsnArg: 1.437 ± 0.035
1.186AsnSer: 1.186 ± 0.029
1.269AsnThr: 1.269 ± 0.032
1.638AsnVal: 1.638 ± 0.04
0.373AsnTrp: 0.373 ± 0.017
0.468AsnTyr: 0.468 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
6.931ProAla: 6.931 ± 0.084
0.297ProCys: 0.297 ± 0.017
4.245ProAsp: 4.245 ± 0.059
3.867ProGlu: 3.867 ± 0.057
1.585ProPhe: 1.585 ± 0.038
5.583ProGly: 5.583 ± 0.068
1.178ProHis: 1.178 ± 0.029
2.276ProIle: 2.276 ± 0.041
1.186ProLys: 1.186 ± 0.026
4.839ProLeu: 4.839 ± 0.061
1.082ProMet: 1.082 ± 0.027
1.199ProAsn: 1.199 ± 0.029
2.713ProPro: 2.713 ± 0.06
1.634ProGln: 1.634 ± 0.032
3.404ProArg: 3.404 ± 0.057
3.246ProSer: 3.246 ± 0.042
3.534ProThr: 3.534 ± 0.056
5.105ProVal: 5.105 ± 0.068
0.795ProTrp: 0.795 ± 0.024
1.024ProTyr: 1.024 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.269GlnAla: 3.269 ± 0.051
0.199GlnCys: 0.199 ± 0.013
1.257GlnAsp: 1.257 ± 0.029
1.158GlnGlu: 1.158 ± 0.026
0.928GlnPhe: 0.928 ± 0.029
1.901GlnGly: 1.901 ± 0.04
0.61GlnHis: 0.61 ± 0.02
1.464GlnIle: 1.464 ± 0.035
0.597GlnLys: 0.597 ± 0.023
3.358GlnLeu: 3.358 ± 0.049
0.658GlnMet: 0.658 ± 0.022
0.595GlnAsn: 0.595 ± 0.024
1.504GlnPro: 1.504 ± 0.032
1.175GlnGln: 1.175 ± 0.033
2.336GlnArg: 2.336 ± 0.044
1.523GlnSer: 1.523 ± 0.034
1.456GlnThr: 1.456 ± 0.036
2.631GlnVal: 2.631 ± 0.039
0.549GlnTrp: 0.549 ± 0.02
0.61GlnTyr: 0.61 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
8.294ArgAla: 8.294 ± 0.099
0.599ArgCys: 0.599 ± 0.022
4.21ArgAsp: 4.21 ± 0.052
4.379ArgGlu: 4.379 ± 0.057
2.387ArgPhe: 2.387 ± 0.043
5.51ArgGly: 5.51 ± 0.071
1.672ArgHis: 1.672 ± 0.036
3.73ArgIle: 3.73 ± 0.048
1.598ArgLys: 1.598 ± 0.038
7.303ArgLeu: 7.303 ± 0.093
1.883ArgMet: 1.883 ± 0.034
1.609ArgAsn: 1.609 ± 0.035
3.857ArgPro: 3.857 ± 0.062
2.076ArgGln: 2.076 ± 0.038
6.63ArgArg: 6.63 ± 0.085
4.447ArgSer: 4.447 ± 0.055
4.705ArgThr: 4.705 ± 0.061
5.838ArgVal: 5.838 ± 0.064
1.26ArgTrp: 1.26 ± 0.029
1.72ArgTyr: 1.72 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
7.413SerAla: 7.413 ± 0.08
0.417SerCys: 0.417 ± 0.018
3.453SerAsp: 3.453 ± 0.048
3.046SerGlu: 3.046 ± 0.051
1.771SerPhe: 1.771 ± 0.035
6.357SerGly: 6.357 ± 0.072
1.036SerHis: 1.036 ± 0.029
2.485SerIle: 2.485 ± 0.042
1.245SerLys: 1.245 ± 0.034
5.271SerLeu: 5.271 ± 0.062
1.463SerMet: 1.463 ± 0.027
1.192SerAsn: 1.192 ± 0.03
3.116SerPro: 3.116 ± 0.049
1.398SerGln: 1.398 ± 0.032
3.978SerArg: 3.978 ± 0.058
3.483SerSer: 3.483 ± 0.056
3.825SerThr: 3.825 ± 0.054
5.116SerVal: 5.116 ± 0.066
0.93SerTrp: 0.93 ± 0.029
1.186SerTyr: 1.186 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
8.007ThrAla: 8.007 ± 0.079
0.427ThrCys: 0.427 ± 0.017
3.726ThrAsp: 3.726 ± 0.062
3.411ThrGlu: 3.411 ± 0.044
1.76ThrPhe: 1.76 ± 0.037
6.086ThrGly: 6.086 ± 0.065
1.21ThrHis: 1.21 ± 0.032
2.465ThrIle: 2.465 ± 0.044
1.211ThrLys: 1.211 ± 0.035
5.837ThrLeu: 5.837 ± 0.068
1.163ThrMet: 1.163 ± 0.025
1.207ThrAsn: 1.207 ± 0.029
3.891ThrPro: 3.891 ± 0.056
1.407ThrGln: 1.407 ± 0.032
3.826ThrArg: 3.826 ± 0.052
3.511ThrSer: 3.511 ± 0.052
4.024ThrThr: 4.024 ± 0.07
6.404ThrVal: 6.404 ± 0.068
0.836ThrTrp: 0.836 ± 0.025
1.273ThrTyr: 1.273 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
11.629ValAla: 11.629 ± 0.121
0.757ValCys: 0.757 ± 0.025
6.011ValAsp: 6.011 ± 0.069
4.945ValGlu: 4.945 ± 0.06
2.649ValPhe: 2.649 ± 0.047
7.427ValGly: 7.427 ± 0.078
1.935ValHis: 1.935 ± 0.039
3.733ValIle: 3.733 ± 0.059
1.573ValLys: 1.573 ± 0.039
9.553ValLeu: 9.553 ± 0.11
1.575ValMet: 1.575 ± 0.033
1.805ValAsn: 1.805 ± 0.037
5.116ValPro: 5.116 ± 0.087
2.284ValGln: 2.284 ± 0.041
6.384ValArg: 6.384 ± 0.08
5.009ValSer: 5.009 ± 0.062
5.831ValThr: 5.831 ± 0.062
8.904ValVal: 8.904 ± 0.1
1.075ValTrp: 1.075 ± 0.028
1.478ValTyr: 1.478 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.591TrpAla: 1.591 ± 0.031
0.143TrpCys: 0.143 ± 0.009
0.773TrpAsp: 0.773 ± 0.026
0.633TrpGlu: 0.633 ± 0.023
0.516TrpPhe: 0.516 ± 0.02
1.012TrpGly: 1.012 ± 0.026
0.317TrpHis: 0.317 ± 0.014
0.702TrpIle: 0.702 ± 0.024
0.33TrpLys: 0.33 ± 0.016
1.593TrpLeu: 1.593 ± 0.034
0.356TrpMet: 0.356 ± 0.016
0.395TrpAsn: 0.395 ± 0.015
0.701TrpPro: 0.701 ± 0.023
0.531TrpGln: 0.531 ± 0.02
1.117TrpArg: 1.117 ± 0.03
0.995TrpSer: 0.995 ± 0.025
0.932TrpThr: 0.932 ± 0.025
1.049TrpVal: 1.049 ± 0.031
0.32TrpTrp: 0.32 ± 0.017
0.299TrpTyr: 0.299 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.251TyrAla: 2.251 ± 0.039
0.19TyrCys: 0.19 ± 0.012
1.208TyrAsp: 1.208 ± 0.033
1.042TyrGlu: 1.042 ± 0.032
0.702TyrPhe: 0.702 ± 0.026
2.0TyrGly: 2.0 ± 0.046
0.378TyrHis: 0.378 ± 0.016
0.687TyrIle: 0.687 ± 0.023
0.333TyrLys: 0.333 ± 0.016
2.163TyrLeu: 2.163 ± 0.041
0.289TyrMet: 0.289 ± 0.015
0.426TyrAsn: 0.426 ± 0.018
1.118TyrPro: 1.118 ± 0.031
0.594TyrGln: 0.594 ± 0.023
1.751TyrArg: 1.751 ± 0.037
1.163TyrSer: 1.163 ± 0.032
1.134TyrThr: 1.134 ± 0.031
1.568TyrVal: 1.568 ± 0.036
0.371TyrTrp: 0.371 ± 0.016
0.427TyrTyr: 0.427 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4379 proteins (1486773 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski