Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella; Salmonella enterica; Salmonella enterica subsp. enterica; Salmonella enterica subsp. enterica serovar Typhimurium

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4538 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0A204|CSGG_SALTY Curli production assembly/transport component CsgG OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=csgG PE=3 SV=1
MM1 pKa = 7.62NMGLFYY7 pKa = 10.89GSSTCYY13 pKa = 10.49TEE15 pKa = 3.84MAAEE19 pKa = 5.11KK20 pKa = 10.26IRR22 pKa = 11.84DD23 pKa = 3.53ILGPEE28 pKa = 4.39LVTLHH33 pKa = 6.14NLKK36 pKa = 10.64DD37 pKa = 3.68DD38 pKa = 4.36APALMEE44 pKa = 4.2QYY46 pKa = 10.98DD47 pKa = 4.12VLILGIPTWDD57 pKa = 3.26FGEE60 pKa = 4.22IQEE63 pKa = 4.35DD64 pKa = 4.04WEE66 pKa = 4.56AVWEE70 pKa = 4.06QLDD73 pKa = 3.96DD74 pKa = 4.48LNLEE78 pKa = 4.23GKK80 pKa = 9.62IVALYY85 pKa = 11.09GMGDD89 pKa = 3.29QLGYY93 pKa = 11.02GEE95 pKa = 4.87WFLDD99 pKa = 3.74ALGMLHH105 pKa = 7.58DD106 pKa = 4.46KK107 pKa = 10.82LALKK111 pKa = 10.16GVKK114 pKa = 9.49FVGYY118 pKa = 9.0WPTEE122 pKa = 3.99GYY124 pKa = 10.58EE125 pKa = 4.14FTSNKK130 pKa = 9.26PVIADD135 pKa = 3.47GQLFVGLALDD145 pKa = 3.64EE146 pKa = 4.38TNQYY150 pKa = 10.89DD151 pKa = 4.3LSDD154 pKa = 3.62EE155 pKa = 5.52RR156 pKa = 11.84IQTWCEE162 pKa = 3.71QILGEE167 pKa = 4.23MAEE170 pKa = 4.64HH171 pKa = 6.46YY172 pKa = 10.67AA173 pKa = 4.29

Molecular weight: 19.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P10369|HIS8_SALTY Histidinol-phosphate aminotransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=hisC PE=3 SV=2
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4533

5

4538

1423640

14

5559

313.7

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.777 ± 0.042

1.155 ± 0.015

5.214 ± 0.037

5.603 ± 0.037

3.863 ± 0.022

7.372 ± 0.034

2.295 ± 0.019

5.931 ± 0.033

4.309 ± 0.032

10.65 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.784 ± 0.021

3.811 ± 0.031

4.466 ± 0.025

4.387 ± 0.027

5.652 ± 0.034

5.818 ± 0.031

5.492 ± 0.035

7.022 ± 0.036

1.523 ± 0.016

2.876 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski