Amino acid dipepetide frequency for Staphylococcus phage StB20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.415AlaAla: 3.415 ± 0.997
0.318AlaCys: 0.318 ± 0.148
2.382AlaAsp: 2.382 ± 0.476
3.653AlaGlu: 3.653 ± 0.628
2.382AlaPhe: 2.382 ± 0.451
2.7AlaGly: 2.7 ± 0.559
1.35AlaHis: 1.35 ± 0.377
4.924AlaIle: 4.924 ± 0.947
5.241AlaLys: 5.241 ± 0.656
3.891AlaLeu: 3.891 ± 0.802
1.668AlaMet: 1.668 ± 0.412
3.574AlaAsn: 3.574 ± 0.544
1.747AlaPro: 1.747 ± 0.326
1.827AlaGln: 1.827 ± 0.299
1.588AlaArg: 1.588 ± 0.334
3.415AlaSer: 3.415 ± 0.7
2.78AlaThr: 2.78 ± 0.49
3.494AlaVal: 3.494 ± 0.543
0.556AlaTrp: 0.556 ± 0.267
1.985AlaTyr: 1.985 ± 0.485
0.0AlaXaa: 0.0 ± 0.0
Cys
0.397CysAla: 0.397 ± 0.165
0.079CysCys: 0.079 ± 0.069
0.159CysAsp: 0.159 ± 0.125
0.397CysGlu: 0.397 ± 0.196
0.238CysPhe: 0.238 ± 0.133
0.635CysGly: 0.635 ± 0.232
0.318CysHis: 0.318 ± 0.15
0.318CysIle: 0.318 ± 0.153
0.794CysLys: 0.794 ± 0.279
0.318CysLeu: 0.318 ± 0.162
0.079CysMet: 0.079 ± 0.075
0.397CysAsn: 0.397 ± 0.148
0.238CysPro: 0.238 ± 0.177
0.159CysGln: 0.159 ± 0.103
0.079CysArg: 0.079 ± 0.066
0.476CysSer: 0.476 ± 0.202
0.397CysThr: 0.397 ± 0.147
0.318CysVal: 0.318 ± 0.141
0.079CysTrp: 0.079 ± 0.077
0.159CysTyr: 0.159 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
2.382AspAla: 2.382 ± 0.483
0.318AspCys: 0.318 ± 0.176
4.765AspAsp: 4.765 ± 0.728
5.797AspGlu: 5.797 ± 0.686
2.78AspPhe: 2.78 ± 0.574
3.018AspGly: 3.018 ± 0.619
0.715AspHis: 0.715 ± 0.205
5.4AspIle: 5.4 ± 0.671
4.844AspLys: 4.844 ± 0.503
5.559AspLeu: 5.559 ± 0.798
2.065AspMet: 2.065 ± 0.35
4.527AspAsn: 4.527 ± 0.648
1.827AspPro: 1.827 ± 0.377
0.874AspGln: 0.874 ± 0.239
2.541AspArg: 2.541 ± 0.527
3.653AspSer: 3.653 ± 0.471
3.891AspThr: 3.891 ± 0.396
4.13AspVal: 4.13 ± 0.614
0.715AspTrp: 0.715 ± 0.251
3.335AspTyr: 3.335 ± 0.444
0.0AspXaa: 0.0 ± 0.0
Glu
3.574GluAla: 3.574 ± 0.438
0.318GluCys: 0.318 ± 0.16
3.971GluAsp: 3.971 ± 0.498
6.75GluGlu: 6.75 ± 1.143
3.256GluPhe: 3.256 ± 0.568
2.541GluGly: 2.541 ± 0.493
1.429GluHis: 1.429 ± 0.338
4.686GluIle: 4.686 ± 0.624
5.956GluLys: 5.956 ± 0.883
6.512GluLeu: 6.512 ± 0.61
2.78GluMet: 2.78 ± 0.455
5.4GluAsn: 5.4 ± 0.774
2.144GluPro: 2.144 ± 0.525
3.415GluGln: 3.415 ± 0.635
2.541GluArg: 2.541 ± 0.497
3.733GluSer: 3.733 ± 0.466
4.686GluThr: 4.686 ± 0.625
5.956GluVal: 5.956 ± 0.7
1.35GluTrp: 1.35 ± 0.42
4.209GluTyr: 4.209 ± 0.645
0.0GluXaa: 0.0 ± 0.0
Phe
2.065PheAla: 2.065 ± 0.335
0.159PheCys: 0.159 ± 0.12
3.415PheAsp: 3.415 ± 0.446
3.097PheGlu: 3.097 ± 0.536
1.668PhePhe: 1.668 ± 0.499
2.78PheGly: 2.78 ± 0.427
0.476PheHis: 0.476 ± 0.173
3.256PheIle: 3.256 ± 0.576
3.733PheLys: 3.733 ± 0.55
2.541PheLeu: 2.541 ± 0.437
1.429PheMet: 1.429 ± 0.416
3.335PheAsn: 3.335 ± 0.522
0.874PhePro: 0.874 ± 0.263
0.556PheGln: 0.556 ± 0.195
1.985PheArg: 1.985 ± 0.357
2.78PheSer: 2.78 ± 0.443
1.747PheThr: 1.747 ± 0.346
2.065PheVal: 2.065 ± 0.439
0.079PheTrp: 0.079 ± 0.074
1.429PheTyr: 1.429 ± 0.319
0.0PheXaa: 0.0 ± 0.0
Gly
3.653GlyAla: 3.653 ± 0.756
0.318GlyCys: 0.318 ± 0.178
3.335GlyAsp: 3.335 ± 0.496
2.621GlyGlu: 2.621 ± 0.691
3.018GlyPhe: 3.018 ± 0.375
4.765GlyGly: 4.765 ± 1.148
1.35GlyHis: 1.35 ± 0.395
5.241GlyIle: 5.241 ± 0.817
6.036GlyLys: 6.036 ± 0.713
4.844GlyLeu: 4.844 ± 0.785
1.668GlyMet: 1.668 ± 0.354
3.415GlyAsn: 3.415 ± 0.651
1.747GlyPro: 1.747 ± 0.497
1.668GlyGln: 1.668 ± 0.351
1.668GlyArg: 1.668 ± 0.341
3.018GlySer: 3.018 ± 0.497
3.812GlyThr: 3.812 ± 0.669
5.559GlyVal: 5.559 ± 0.699
1.191GlyTrp: 1.191 ± 0.426
3.256GlyTyr: 3.256 ± 0.526
0.0GlyXaa: 0.0 ± 0.0
His
1.112HisAla: 1.112 ± 0.242
0.159HisCys: 0.159 ± 0.1
0.953HisAsp: 0.953 ± 0.323
0.794HisGlu: 0.794 ± 0.238
0.874HisPhe: 0.874 ± 0.287
1.35HisGly: 1.35 ± 0.377
0.476HisHis: 0.476 ± 0.188
1.35HisIle: 1.35 ± 0.375
1.35HisLys: 1.35 ± 0.363
1.509HisLeu: 1.509 ± 0.443
0.079HisMet: 0.079 ± 0.087
1.35HisAsn: 1.35 ± 0.324
0.874HisPro: 0.874 ± 0.242
0.635HisGln: 0.635 ± 0.262
0.953HisArg: 0.953 ± 0.28
1.112HisSer: 1.112 ± 0.228
0.953HisThr: 0.953 ± 0.277
0.953HisVal: 0.953 ± 0.306
0.397HisTrp: 0.397 ± 0.204
0.794HisTyr: 0.794 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
4.209IleAla: 4.209 ± 0.476
0.794IleCys: 0.794 ± 0.253
5.48IleAsp: 5.48 ± 0.572
6.83IleGlu: 6.83 ± 0.813
2.859IlePhe: 2.859 ± 0.534
4.05IleGly: 4.05 ± 0.561
1.588IleHis: 1.588 ± 0.492
4.05IleIle: 4.05 ± 0.628
7.862IleLys: 7.862 ± 1.204
3.891IleLeu: 3.891 ± 0.457
1.271IleMet: 1.271 ± 0.42
5.877IleAsn: 5.877 ± 0.819
2.78IlePro: 2.78 ± 0.401
2.382IleGln: 2.382 ± 0.472
2.7IleArg: 2.7 ± 0.464
4.765IleSer: 4.765 ± 0.592
4.13IleThr: 4.13 ± 0.562
3.574IleVal: 3.574 ± 0.506
0.953IleTrp: 0.953 ± 0.337
2.462IleTyr: 2.462 ± 0.453
0.0IleXaa: 0.0 ± 0.0
Lys
4.368LysAla: 4.368 ± 0.868
0.476LysCys: 0.476 ± 0.209
6.353LysAsp: 6.353 ± 0.611
6.83LysGlu: 6.83 ± 0.926
2.541LysPhe: 2.541 ± 0.403
7.544LysGly: 7.544 ± 0.836
1.429LysHis: 1.429 ± 0.303
5.718LysIle: 5.718 ± 0.764
8.497LysLys: 8.497 ± 0.891
7.306LysLeu: 7.306 ± 0.598
2.065LysMet: 2.065 ± 0.35
6.115LysAsn: 6.115 ± 0.807
3.653LysPro: 3.653 ± 0.498
4.05LysGln: 4.05 ± 0.651
4.368LysArg: 4.368 ± 0.607
6.115LysSer: 6.115 ± 0.808
5.241LysThr: 5.241 ± 0.49
5.877LysVal: 5.877 ± 0.561
1.35LysTrp: 1.35 ± 0.333
4.288LysTyr: 4.288 ± 0.506
0.0LysXaa: 0.0 ± 0.0
Leu
3.971LeuAla: 3.971 ± 0.641
0.397LeuCys: 0.397 ± 0.167
5.639LeuAsp: 5.639 ± 0.648
5.559LeuGlu: 5.559 ± 0.621
2.541LeuPhe: 2.541 ± 0.532
4.447LeuGly: 4.447 ± 0.661
1.429LeuHis: 1.429 ± 0.337
5.083LeuIle: 5.083 ± 0.648
7.386LeuLys: 7.386 ± 0.591
5.083LeuLeu: 5.083 ± 0.597
1.906LeuMet: 1.906 ± 0.543
5.241LeuAsn: 5.241 ± 0.546
2.621LeuPro: 2.621 ± 0.519
3.097LeuGln: 3.097 ± 0.476
3.971LeuArg: 3.971 ± 0.631
4.686LeuSer: 4.686 ± 0.581
4.686LeuThr: 4.686 ± 0.522
4.05LeuVal: 4.05 ± 0.569
0.794LeuTrp: 0.794 ± 0.365
2.938LeuTyr: 2.938 ± 0.474
0.0LeuXaa: 0.0 ± 0.0
Met
1.906MetAla: 1.906 ± 0.4
0.238MetCys: 0.238 ± 0.141
1.112MetAsp: 1.112 ± 0.262
2.303MetGlu: 2.303 ± 0.377
1.032MetPhe: 1.032 ± 0.293
1.112MetGly: 1.112 ± 0.29
0.238MetHis: 0.238 ± 0.134
1.588MetIle: 1.588 ± 0.263
3.574MetLys: 3.574 ± 0.508
1.985MetLeu: 1.985 ± 0.479
0.794MetMet: 0.794 ± 0.256
2.382MetAsn: 2.382 ± 0.397
0.953MetPro: 0.953 ± 0.241
0.794MetGln: 0.794 ± 0.229
1.668MetArg: 1.668 ± 0.509
1.588MetSer: 1.588 ± 0.375
1.906MetThr: 1.906 ± 0.357
1.032MetVal: 1.032 ± 0.268
0.397MetTrp: 0.397 ± 0.178
0.794MetTyr: 0.794 ± 0.256
0.0MetXaa: 0.0 ± 0.0
Asn
3.971AsnAla: 3.971 ± 0.71
0.397AsnCys: 0.397 ± 0.147
4.606AsnAsp: 4.606 ± 0.735
5.241AsnGlu: 5.241 ± 0.611
2.541AsnPhe: 2.541 ± 0.381
6.036AsnGly: 6.036 ± 0.862
1.112AsnHis: 1.112 ± 0.338
3.335AsnIle: 3.335 ± 0.499
5.321AsnLys: 5.321 ± 0.758
5.877AsnLeu: 5.877 ± 0.563
1.429AsnMet: 1.429 ± 0.316
4.447AsnAsn: 4.447 ± 0.831
2.144AsnPro: 2.144 ± 0.511
2.859AsnGln: 2.859 ± 0.457
3.018AsnArg: 3.018 ± 0.52
3.097AsnSer: 3.097 ± 0.457
5.083AsnThr: 5.083 ± 0.588
3.812AsnVal: 3.812 ± 0.589
1.032AsnTrp: 1.032 ± 0.32
3.494AsnTyr: 3.494 ± 0.473
0.0AsnXaa: 0.0 ± 0.0
Pro
1.032ProAla: 1.032 ± 0.326
0.0ProCys: 0.0 ± 0.0
1.35ProAsp: 1.35 ± 0.327
3.097ProGlu: 3.097 ± 0.673
1.668ProPhe: 1.668 ± 0.495
1.509ProGly: 1.509 ± 0.488
0.715ProHis: 0.715 ± 0.28
2.224ProIle: 2.224 ± 0.433
3.812ProLys: 3.812 ± 0.569
2.541ProLeu: 2.541 ± 0.611
0.794ProMet: 0.794 ± 0.205
1.985ProAsn: 1.985 ± 0.397
1.35ProPro: 1.35 ± 0.371
1.191ProGln: 1.191 ± 0.417
0.476ProArg: 0.476 ± 0.174
1.668ProSer: 1.668 ± 0.399
2.224ProThr: 2.224 ± 0.358
1.588ProVal: 1.588 ± 0.276
0.079ProTrp: 0.079 ± 0.074
1.906ProTyr: 1.906 ± 0.391
0.0ProXaa: 0.0 ± 0.0
Gln
2.621GlnAla: 2.621 ± 0.417
0.238GlnCys: 0.238 ± 0.134
1.985GlnAsp: 1.985 ± 0.478
2.303GlnGlu: 2.303 ± 0.487
0.953GlnPhe: 0.953 ± 0.279
2.541GlnGly: 2.541 ± 0.885
0.556GlnHis: 0.556 ± 0.214
3.097GlnIle: 3.097 ± 0.454
3.256GlnLys: 3.256 ± 0.498
2.938GlnLeu: 2.938 ± 0.371
1.271GlnMet: 1.271 ± 0.329
1.906GlnAsn: 1.906 ± 0.463
0.874GlnPro: 0.874 ± 0.26
2.462GlnGln: 2.462 ± 0.538
2.462GlnArg: 2.462 ± 0.487
2.224GlnSer: 2.224 ± 0.474
2.303GlnThr: 2.303 ± 0.482
2.224GlnVal: 2.224 ± 0.395
0.318GlnTrp: 0.318 ± 0.142
0.953GlnTyr: 0.953 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
1.827ArgAla: 1.827 ± 0.349
0.0ArgCys: 0.0 ± 0.0
2.859ArgAsp: 2.859 ± 0.607
2.938ArgGlu: 2.938 ± 0.486
1.271ArgPhe: 1.271 ± 0.249
2.382ArgGly: 2.382 ± 0.314
0.953ArgHis: 0.953 ± 0.268
2.78ArgIle: 2.78 ± 0.391
3.891ArgLys: 3.891 ± 0.613
3.256ArgLeu: 3.256 ± 0.501
1.35ArgMet: 1.35 ± 0.308
3.574ArgAsn: 3.574 ± 0.56
1.032ArgPro: 1.032 ± 0.303
1.906ArgGln: 1.906 ± 0.416
1.429ArgArg: 1.429 ± 0.442
1.985ArgSer: 1.985 ± 0.377
2.462ArgThr: 2.462 ± 0.448
1.985ArgVal: 1.985 ± 0.415
0.715ArgTrp: 0.715 ± 0.304
2.065ArgTyr: 2.065 ± 0.356
0.0ArgXaa: 0.0 ± 0.0
Ser
2.224SerAla: 2.224 ± 0.363
0.476SerCys: 0.476 ± 0.174
4.765SerAsp: 4.765 ± 0.618
4.288SerGlu: 4.288 ± 0.567
2.938SerPhe: 2.938 ± 0.449
3.733SerGly: 3.733 ± 0.675
0.556SerHis: 0.556 ± 0.205
4.13SerIle: 4.13 ± 0.62
6.036SerLys: 6.036 ± 0.873
4.05SerLeu: 4.05 ± 0.474
2.144SerMet: 2.144 ± 0.417
3.653SerAsn: 3.653 ± 0.532
1.35SerPro: 1.35 ± 0.369
2.938SerGln: 2.938 ± 0.467
2.462SerArg: 2.462 ± 0.475
3.097SerSer: 3.097 ± 0.487
3.891SerThr: 3.891 ± 0.485
4.209SerVal: 4.209 ± 0.492
0.397SerTrp: 0.397 ± 0.192
1.588SerTyr: 1.588 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
3.971ThrAla: 3.971 ± 0.636
0.397ThrCys: 0.397 ± 0.157
2.859ThrAsp: 2.859 ± 0.486
3.812ThrGlu: 3.812 ± 0.602
2.621ThrPhe: 2.621 ± 0.478
3.653ThrGly: 3.653 ± 0.858
1.747ThrHis: 1.747 ± 0.389
5.797ThrIle: 5.797 ± 0.634
4.924ThrLys: 4.924 ± 0.592
4.288ThrLeu: 4.288 ± 0.607
1.747ThrMet: 1.747 ± 0.397
3.494ThrAsn: 3.494 ± 0.551
2.224ThrPro: 2.224 ± 0.506
2.303ThrGln: 2.303 ± 0.349
1.588ThrArg: 1.588 ± 0.35
4.288ThrSer: 4.288 ± 0.639
3.256ThrThr: 3.256 ± 0.579
4.13ThrVal: 4.13 ± 0.718
0.794ThrTrp: 0.794 ± 0.207
2.144ThrTyr: 2.144 ± 0.45
0.0ThrXaa: 0.0 ± 0.0
Val
3.653ValAla: 3.653 ± 0.539
0.238ValCys: 0.238 ± 0.131
3.812ValAsp: 3.812 ± 0.762
4.368ValGlu: 4.368 ± 0.605
2.303ValPhe: 2.303 ± 0.496
4.13ValGly: 4.13 ± 0.647
0.556ValHis: 0.556 ± 0.173
5.4ValIle: 5.4 ± 0.703
6.353ValLys: 6.353 ± 0.678
4.686ValLeu: 4.686 ± 0.398
1.827ValMet: 1.827 ± 0.384
3.415ValAsn: 3.415 ± 0.462
1.588ValPro: 1.588 ± 0.342
2.144ValGln: 2.144 ± 0.351
2.7ValArg: 2.7 ± 0.326
4.209ValSer: 4.209 ± 0.496
4.686ValThr: 4.686 ± 0.525
2.859ValVal: 2.859 ± 0.425
0.874ValTrp: 0.874 ± 0.314
2.144ValTyr: 2.144 ± 0.407
0.0ValXaa: 0.0 ± 0.0
Trp
0.476TrpAla: 0.476 ± 0.211
0.079TrpCys: 0.079 ± 0.079
0.476TrpAsp: 0.476 ± 0.186
1.271TrpGlu: 1.271 ± 0.304
0.318TrpPhe: 0.318 ± 0.15
0.874TrpGly: 0.874 ± 0.239
0.238TrpHis: 0.238 ± 0.128
1.112TrpIle: 1.112 ± 0.293
0.874TrpLys: 0.874 ± 0.243
0.953TrpLeu: 0.953 ± 0.287
0.318TrpMet: 0.318 ± 0.17
1.747TrpAsn: 1.747 ± 0.678
0.397TrpPro: 0.397 ± 0.211
0.476TrpGln: 0.476 ± 0.165
0.635TrpArg: 0.635 ± 0.241
0.556TrpSer: 0.556 ± 0.224
0.635TrpThr: 0.635 ± 0.252
1.032TrpVal: 1.032 ± 0.329
0.0TrpTrp: 0.0 ± 0.0
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.065TyrAla: 2.065 ± 0.405
0.635TyrCys: 0.635 ± 0.272
2.859TyrAsp: 2.859 ± 0.522
3.256TyrGlu: 3.256 ± 0.463
1.668TyrPhe: 1.668 ± 0.411
2.382TyrGly: 2.382 ± 0.42
0.794TyrHis: 0.794 ± 0.233
3.018TyrIle: 3.018 ± 0.626
4.527TyrLys: 4.527 ± 0.692
3.494TyrLeu: 3.494 ± 0.419
0.635TyrMet: 0.635 ± 0.186
3.018TyrAsn: 3.018 ± 0.493
0.794TyrPro: 0.794 ± 0.321
1.747TyrGln: 1.747 ± 0.313
1.827TyrArg: 1.827 ± 0.317
2.462TyrSer: 2.462 ± 0.412
1.271TyrThr: 1.271 ± 0.374
3.097TyrVal: 3.097 ± 0.392
0.397TyrTrp: 0.397 ± 0.169
1.906TyrTyr: 1.906 ± 0.438
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (12593 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski