Bifidobacterium minimum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1589 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087BM83|A0A087BM83_9BIFI ATP synthase subunit beta OS=Bifidobacterium minimum OX=1693 GN=atpD PE=3 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.51YY5 pKa = 10.08DD6 pKa = 3.9SPRR9 pKa = 11.84NKK11 pKa = 10.59DD12 pKa = 3.51DD13 pKa = 4.87DD14 pKa = 5.01DD15 pKa = 4.52SLEE18 pKa = 4.05EE19 pKa = 4.21LGKK22 pKa = 9.29GTRR25 pKa = 11.84PSAADD30 pKa = 3.64LEE32 pKa = 4.48EE33 pKa = 6.83DD34 pKa = 3.8DD35 pKa = 5.92DD36 pKa = 4.79EE37 pKa = 4.77NAVASSYY44 pKa = 11.18DD45 pKa = 3.7VYY47 pKa = 11.47SGADD51 pKa = 3.4VNSEE55 pKa = 4.02DD56 pKa = 4.49SSITVTPTQSDD67 pKa = 3.46EE68 pKa = 4.16FLCYY72 pKa = 10.27GCFLIKK78 pKa = 10.22HH79 pKa = 6.5RR80 pKa = 11.84SQLARR85 pKa = 11.84TDD87 pKa = 3.67ATGHH91 pKa = 6.81DD92 pKa = 4.06YY93 pKa = 11.19CKK95 pKa = 10.66EE96 pKa = 4.06CVDD99 pKa = 3.49GDD101 pKa = 3.74GGYY104 pKa = 10.72VLL106 pKa = 5.42

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087BPP0|A0A087BPP0_9BIFI Uncharacterized protein OS=Bifidobacterium minimum OX=1693 GN=BMIN_0703 PE=4 SV=1
MM1 pKa = 7.4TFNDD5 pKa = 4.45RR6 pKa = 11.84IIPASAGQTYY16 pKa = 10.19SWDD19 pKa = 3.51PKK21 pKa = 10.94ASDD24 pKa = 3.71NGDD27 pKa = 3.34HH28 pKa = 6.79PRR30 pKa = 11.84EE31 pKa = 4.4CGANACQLTLSASRR45 pKa = 11.84RR46 pKa = 11.84GSSPRR51 pKa = 11.84VRR53 pKa = 11.84GKK55 pKa = 8.95RR56 pKa = 11.84TPPGRR61 pKa = 11.84GTRR64 pKa = 11.84QRR66 pKa = 11.84GIIPASAGQTDD77 pKa = 3.74WLLFPMAYY85 pKa = 10.2SSDD88 pKa = 3.42HH89 pKa = 6.13PRR91 pKa = 11.84EE92 pKa = 4.3CGANLPFAATAAQRR106 pKa = 11.84FGSSPRR112 pKa = 11.84VRR114 pKa = 11.84GKK116 pKa = 9.85HH117 pKa = 6.06LRR119 pKa = 11.84DD120 pKa = 3.53AEE122 pKa = 3.95QAAINRR128 pKa = 11.84IIPASAGQTLIGLVYY143 pKa = 9.2WSSARR148 pKa = 11.84DD149 pKa = 3.48HH150 pKa = 6.63PRR152 pKa = 11.84EE153 pKa = 4.25CGANSHH159 pKa = 5.36TLQRR163 pKa = 11.84KK164 pKa = 7.24PPARR168 pKa = 11.84RR169 pKa = 11.84RR170 pKa = 11.84IFFNLDD176 pKa = 3.12SQIINAINTNIKK188 pKa = 9.87PMQSYY193 pKa = 9.98PLFSTHH199 pKa = 5.77RR200 pKa = 11.84TPHH203 pKa = 5.97LRR205 pKa = 11.84SLPLSSNAPKK215 pKa = 10.12QLPPKK220 pKa = 9.14HH221 pKa = 6.54HH222 pKa = 6.44SASMFTVQTAGIVRR236 pKa = 11.84PCSIPPACHH245 pKa = 6.06PNVVVSHH252 pKa = 6.11CCWVVEE258 pKa = 4.2

Molecular weight: 28.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1589

0

1589

545536

30

3223

343.3

37.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.587 ± 0.079

0.888 ± 0.02

7.08 ± 0.068

5.106 ± 0.056

3.047 ± 0.034

8.492 ± 0.056

2.272 ± 0.03

5.152 ± 0.051

2.823 ± 0.055

8.644 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.718 ± 0.026

2.61 ± 0.044

4.805 ± 0.044

2.851 ± 0.038

7.461 ± 0.082

7.322 ± 0.063

6.099 ± 0.058

8.35 ± 0.067

1.299 ± 0.026

2.394 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski