Haemonchus placei (Barber s pole worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Strongylida; Trichostrongyloidea; Haemonchidae; Haemonchus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A158QJR8|A0A158QJR8_HAEPC Ribulose-phosphate 3-epimerase OS=Haemonchus placei OX=6290 GN=HPLM_LOCUS3258 PE=3 SV=1
MM1 pKa = 8.14DD2 pKa = 4.34EE3 pKa = 4.8GGYY6 pKa = 8.43EE7 pKa = 4.26VCPDD11 pKa = 3.5MTPSQLEE18 pKa = 3.88RR19 pKa = 11.84AKK21 pKa = 11.45NMDD24 pKa = 4.65DD25 pKa = 4.4GAACNQLMTVFIALNRR41 pKa = 11.84IDD43 pKa = 5.2PYY45 pKa = 10.82MDD47 pKa = 4.74DD48 pKa = 3.49GVMAEE53 pKa = 4.72TEE55 pKa = 4.19PADD58 pKa = 4.22DD59 pKa = 4.39ACTLTTSEE67 pKa = 4.47SGHH70 pKa = 6.23FEE72 pKa = 3.96AALLNLKK79 pKa = 8.68GTMDD83 pKa = 3.21EE84 pKa = 4.99GGYY87 pKa = 8.46EE88 pKa = 4.35VCPDD92 pKa = 3.5MTPSQLEE99 pKa = 3.88RR100 pKa = 11.84AKK102 pKa = 11.45NMDD105 pKa = 4.65DD106 pKa = 4.4GAACNQLMTVLL117 pKa = 4.33

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P8CSI8|A0A3P8CSI8_HAEPC Peroxin/Ferlin domain-containing protein (Fragment) OS=Haemonchus placei OX=6290 GN=HPLM_LOCUS21605 PE=4 SV=1
MM1 pKa = 7.31NPHH4 pKa = 6.71RR5 pKa = 11.84PPRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84PPLLNRR16 pKa = 11.84HH17 pKa = 4.81QPLRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84PPLPNQHH30 pKa = 6.28RR31 pKa = 11.84PPRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84QQLNPPRR44 pKa = 11.84PPRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84PPLPNPHH56 pKa = 6.67RR57 pKa = 11.84PPRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84QQLNPHH69 pKa = 6.51RR70 pKa = 11.84PPRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84PPLPNPHH82 pKa = 6.54RR83 pKa = 11.84PLRR86 pKa = 11.84RR87 pKa = 11.84LFSCILLRR95 pKa = 11.84MLLLMM100 pKa = 5.66

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21409

0

21409

5856531

31

6901

273.6

30.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.964 ± 0.019

2.234 ± 0.017

5.297 ± 0.014

6.425 ± 0.026

4.334 ± 0.016

5.842 ± 0.028

2.425 ± 0.01

5.456 ± 0.014

5.529 ± 0.024

9.089 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.009

4.041 ± 0.011

5.19 ± 0.026

3.83 ± 0.015

6.174 ± 0.019

7.88 ± 0.022

5.571 ± 0.015

6.714 ± 0.014

1.19 ± 0.007

3.063 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski