Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Sulfurisphaera; Sulfurisphaera tokodaii

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2805 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q971C1|SYM_SULTO Methionine--tRNA ligase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=metG PE=3 SV=1
MM1 pKa = 7.5QNTTKK6 pKa = 10.96ALIIGTVLGILLISFVASMTFYY28 pKa = 10.52YY29 pKa = 7.48TTQPRR34 pKa = 11.84DD35 pKa = 2.72VDD37 pKa = 3.57ATVVGDD43 pKa = 4.02ASANIALIANDD54 pKa = 3.58TAIGYY59 pKa = 9.78SSTGSPFVTYY69 pKa = 10.6NSQGDD74 pKa = 3.73LVINFGNVATNANEE88 pKa = 3.92TLKK91 pKa = 11.13YY92 pKa = 10.83LFDD95 pKa = 3.9VKK97 pKa = 11.27NNLNVTVSVTVTIPSPTISGNSLYY121 pKa = 10.33IFTPSGGVSTYY132 pKa = 11.21SFTLAPGAEE141 pKa = 4.14MPISLEE147 pKa = 3.98LLTGGTQGSVSFSIVVEE164 pKa = 4.35ATAVPPSSS172 pKa = 3.31

Molecular weight: 17.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9VNI5|F9VNI5_SULTO Putative dUTP diphosphatase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=ST1632 PE=1 SV=1
MM1 pKa = 7.64CPRR4 pKa = 11.84TEE6 pKa = 3.86LHH8 pKa = 6.35IPEE11 pKa = 4.35SPRR14 pKa = 11.84AKK16 pKa = 10.4SRR18 pKa = 11.84GQLLVRR24 pKa = 11.84DD25 pKa = 4.32RR26 pKa = 11.84GNGLKK31 pKa = 8.1TQPVVYY37 pKa = 9.74RR38 pKa = 11.84WTNGAGWVCITPTSYY53 pKa = 10.66EE54 pKa = 4.08VVRR57 pKa = 11.84MKK59 pKa = 10.61VVNHH63 pKa = 6.57KK64 pKa = 10.2PMNRR68 pKa = 11.84PKK70 pKa = 10.26GTLALL75 pKa = 4.14

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2805

0

2805

757948

31

1442

270.2

30.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.568 ± 0.049

0.632 ± 0.018

4.692 ± 0.043

7.158 ± 0.058

4.465 ± 0.043

6.326 ± 0.045

1.294 ± 0.018

9.952 ± 0.048

8.088 ± 0.066

10.254 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.022

4.872 ± 0.044

3.87 ± 0.03

2.092 ± 0.025

4.172 ± 0.036

6.531 ± 0.047

4.736 ± 0.042

7.237 ± 0.043

1.022 ± 0.016

4.919 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski