Oryza sativa subsp. indica (Rice)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae;

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37391 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A2ZKD5|A2ZKD5_ORYSI Uncharacterized protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_38289 PE=4 SV=1
MM1 pKa = 7.46EE2 pKa = 6.25ADD4 pKa = 4.51DD5 pKa = 5.13DD6 pKa = 4.69VSTAALTDD14 pKa = 3.75GGHH17 pKa = 5.17GQRR20 pKa = 11.84HH21 pKa = 5.88RR22 pKa = 11.84FTFLLPSQNAMMPPAPLTSIDD43 pKa = 5.39DD44 pKa = 3.96EE45 pKa = 7.3DD46 pKa = 6.52DD47 pKa = 4.38DD48 pKa = 5.57FSPDD52 pKa = 3.4DD53 pKa = 3.48VAQILSHH60 pKa = 6.81LLLNGVISGEE70 pKa = 4.24TALLLQILTVALHH83 pKa = 6.51FDD85 pKa = 3.55LGGGGGGGGHH95 pKa = 5.93GQNNDD100 pKa = 3.38EE101 pKa = 4.84DD102 pKa = 5.59AMMAAPPPSIDD113 pKa = 5.95DD114 pKa = 4.01EE115 pKa = 7.14DD116 pKa = 6.7DD117 pKa = 4.98DD118 pKa = 6.21DD119 pKa = 6.1GSPLLDD125 pKa = 3.57QVLCYY130 pKa = 10.68LLLNGIISGGRR141 pKa = 11.84ALQILQNANMPLDD154 pKa = 4.92LDD156 pKa = 4.79LDD158 pKa = 4.47DD159 pKa = 5.89GGANMPLNLDD169 pKa = 3.68DD170 pKa = 5.04GGGFRR175 pKa = 11.84GVPASAAAVAGLEE188 pKa = 4.06KK189 pKa = 10.55QVFHH193 pKa = 6.84QFDD196 pKa = 3.46HH197 pKa = 7.13HH198 pKa = 7.77GGDD201 pKa = 5.27DD202 pKa = 5.04DD203 pKa = 7.14DD204 pKa = 6.84DD205 pKa = 6.95DD206 pKa = 4.16EE207 pKa = 6.75AKK209 pKa = 10.77DD210 pKa = 3.65SAAGCVICMEE220 pKa = 4.39EE221 pKa = 4.49FVAGDD226 pKa = 3.8KK227 pKa = 10.85VCAIPCAGNHH237 pKa = 5.69SFHH240 pKa = 6.58HH241 pKa = 5.99HH242 pKa = 7.39CITEE246 pKa = 3.92WLGCSNVCPLCRR258 pKa = 11.84HH259 pKa = 6.44ALPVEE264 pKa = 4.21EE265 pKa = 4.25QDD267 pKa = 4.12EE268 pKa = 4.77GGATT272 pKa = 3.39

Molecular weight: 28.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8AZW2|B8AZW2_ORYSI Rx_N domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_19128 PE=3 SV=1
MM1 pKa = 7.81APTLPSARR9 pKa = 11.84RR10 pKa = 11.84TSAPPTASRR19 pKa = 11.84CHH21 pKa = 5.62PWRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84GRR28 pKa = 11.84ARR30 pKa = 11.84RR31 pKa = 11.84SSPPLLTFLFPIGLLPDD48 pKa = 4.08RR49 pKa = 11.84LPRR52 pKa = 11.84PTTPPFQRR60 pKa = 11.84SAATVILFQTRR71 pKa = 11.84ARR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84WLPPSSKK82 pKa = 10.02RR83 pKa = 11.84SRR85 pKa = 11.84RR86 pKa = 11.84WLGFAPPSSAKK97 pKa = 10.01RR98 pKa = 11.84PRR100 pKa = 11.84RR101 pKa = 3.53

Molecular weight: 11.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37344

47

37391

13937853

29

5024

372.8

40.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.471 ± 0.02

1.896 ± 0.006

5.506 ± 0.009

6.024 ± 0.014

3.602 ± 0.009

7.557 ± 0.015

2.472 ± 0.005

4.361 ± 0.01

4.828 ± 0.012

9.463 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.005

3.493 ± 0.01

5.479 ± 0.012

3.38 ± 0.01

6.389 ± 0.013

8.142 ± 0.013

4.77 ± 0.008

6.838 ± 0.009

1.333 ± 0.005

2.541 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski