Amino acid dipepetide frequency for Bifidobacterium actinocoloniiforme DSM 22766

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.063AlaAla: 13.063 ± 0.234
1.176AlaCys: 1.176 ± 0.053
6.729AlaAsp: 6.729 ± 0.124
5.363AlaGlu: 5.363 ± 0.131
3.613AlaPhe: 3.613 ± 0.087
9.99AlaGly: 9.99 ± 0.169
1.976AlaHis: 1.976 ± 0.058
5.319AlaIle: 5.319 ± 0.105
4.546AlaLys: 4.546 ± 0.112
11.36AlaLeu: 11.36 ± 0.208
2.944AlaMet: 2.944 ± 0.089
2.856AlaAsn: 2.856 ± 0.086
4.84AlaPro: 4.84 ± 0.127
5.68AlaGln: 5.68 ± 0.12
7.259AlaArg: 7.259 ± 0.134
7.647AlaSer: 7.647 ± 0.137
5.081AlaThr: 5.081 ± 0.124
7.795AlaVal: 7.795 ± 0.123
1.796AlaTrp: 1.796 ± 0.074
2.763AlaTyr: 2.763 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
0.931CysAla: 0.931 ± 0.045
0.112CysCys: 0.112 ± 0.016
0.467CysAsp: 0.467 ± 0.032
0.426CysGlu: 0.426 ± 0.026
0.296CysPhe: 0.296 ± 0.024
1.032CysGly: 1.032 ± 0.051
0.177CysHis: 0.177 ± 0.018
0.352CysIle: 0.352 ± 0.027
0.238CysLys: 0.238 ± 0.025
0.973CysLeu: 0.973 ± 0.057
0.188CysMet: 0.188 ± 0.018
0.175CysAsn: 0.175 ± 0.018
0.492CysPro: 0.492 ± 0.036
0.333CysGln: 0.333 ± 0.025
0.523CysArg: 0.523 ± 0.031
0.53CysSer: 0.53 ± 0.034
0.418CysThr: 0.418 ± 0.03
0.65CysVal: 0.65 ± 0.035
0.105CysTrp: 0.105 ± 0.015
0.238CysTyr: 0.238 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
6.452AspAla: 6.452 ± 0.14
0.528AspCys: 0.528 ± 0.034
3.894AspAsp: 3.894 ± 0.112
4.207AspGlu: 4.207 ± 0.114
2.237AspPhe: 2.237 ± 0.073
5.722AspGly: 5.722 ± 0.134
1.319AspHis: 1.319 ± 0.051
2.56AspIle: 2.56 ± 0.081
2.33AspLys: 2.33 ± 0.082
6.032AspLeu: 6.032 ± 0.122
1.545AspMet: 1.545 ± 0.05
1.281AspAsn: 1.281 ± 0.057
3.841AspPro: 3.841 ± 0.071
3.018AspGln: 3.018 ± 0.08
3.879AspArg: 3.879 ± 0.112
3.573AspSer: 3.573 ± 0.085
2.643AspThr: 2.643 ± 0.078
4.036AspVal: 4.036 ± 0.093
1.013AspTrp: 1.013 ± 0.043
1.893AspTyr: 1.893 ± 0.072
0.0AspXaa: 0.0 ± 0.0
Glu
6.4GluAla: 6.4 ± 0.13
0.365GluCys: 0.365 ± 0.026
3.459GluAsp: 3.459 ± 0.102
3.329GluGlu: 3.329 ± 0.109
1.56GluPhe: 1.56 ± 0.056
4.715GluGly: 4.715 ± 0.102
1.38GluHis: 1.38 ± 0.045
2.128GluIle: 2.128 ± 0.067
1.577GluLys: 1.577 ± 0.074
5.059GluLeu: 5.059 ± 0.115
1.157GluMet: 1.157 ± 0.044
1.344GluAsn: 1.344 ± 0.054
2.966GluPro: 2.966 ± 0.08
2.7GluGln: 2.7 ± 0.077
5.154GluArg: 5.154 ± 0.152
3.225GluSer: 3.225 ± 0.084
2.514GluThr: 2.514 ± 0.072
3.428GluVal: 3.428 ± 0.088
0.555GluTrp: 0.555 ± 0.035
1.363GluTyr: 1.363 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.432PheAla: 3.432 ± 0.086
0.327PheCys: 0.327 ± 0.025
2.332PheAsp: 2.332 ± 0.073
1.625PheGlu: 1.625 ± 0.052
1.131PhePhe: 1.131 ± 0.047
3.137PheGly: 3.137 ± 0.077
0.646PheHis: 0.646 ± 0.034
1.625PheIle: 1.625 ± 0.063
1.114PheLys: 1.114 ± 0.047
2.896PheLeu: 2.896 ± 0.099
0.775PheMet: 0.775 ± 0.046
1.091PheAsn: 1.091 ± 0.047
1.29PhePro: 1.29 ± 0.048
1.091PheGln: 1.091 ± 0.043
1.594PheArg: 1.594 ± 0.056
2.271PheSer: 2.271 ± 0.075
2.054PheThr: 2.054 ± 0.075
2.292PheVal: 2.292 ± 0.079
0.412PheTrp: 0.412 ± 0.033
0.849PheTyr: 0.849 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
8.542GlyAla: 8.542 ± 0.151
0.792GlyCys: 0.792 ± 0.044
4.882GlyAsp: 4.882 ± 0.116
4.447GlyGlu: 4.447 ± 0.107
3.067GlyPhe: 3.067 ± 0.079
7.043GlyGly: 7.043 ± 0.164
1.957GlyHis: 1.957 ± 0.067
4.091GlyIle: 4.091 ± 0.099
3.966GlyLys: 3.966 ± 0.099
8.658GlyLeu: 8.658 ± 0.148
2.223GlyMet: 2.223 ± 0.072
2.305GlyAsn: 2.305 ± 0.09
3.772GlyPro: 3.772 ± 0.097
4.354GlyGln: 4.354 ± 0.12
6.225GlyArg: 6.225 ± 0.124
6.547GlySer: 6.547 ± 0.12
4.181GlyThr: 4.181 ± 0.11
6.286GlyVal: 6.286 ± 0.129
1.399GlyTrp: 1.399 ± 0.057
2.472GlyTyr: 2.472 ± 0.077
0.0GlyXaa: 0.0 ± 0.0
His
1.965HisAla: 1.965 ± 0.072
0.156HisCys: 0.156 ± 0.018
1.237HisAsp: 1.237 ± 0.046
1.079HisGlu: 1.079 ± 0.041
0.562HisPhe: 0.562 ± 0.034
1.832HisGly: 1.832 ± 0.07
0.462HisHis: 0.462 ± 0.031
0.918HisIle: 0.918 ± 0.042
0.601HisLys: 0.601 ± 0.035
1.638HisLeu: 1.638 ± 0.052
0.492HisMet: 0.492 ± 0.036
0.523HisAsn: 0.523 ± 0.03
1.306HisPro: 1.306 ± 0.044
0.806HisGln: 0.806 ± 0.039
1.465HisArg: 1.465 ± 0.058
1.268HisSer: 1.268 ± 0.058
0.994HisThr: 0.994 ± 0.051
1.427HisVal: 1.427 ± 0.046
0.344HisTrp: 0.344 ± 0.028
0.591HisTyr: 0.591 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
5.416IleAla: 5.416 ± 0.117
0.53IleCys: 0.53 ± 0.035
3.31IleAsp: 3.31 ± 0.089
2.917IleGlu: 2.917 ± 0.079
1.539IlePhe: 1.539 ± 0.062
4.169IleGly: 4.169 ± 0.093
0.918IleHis: 0.918 ± 0.047
2.41IleIle: 2.41 ± 0.091
1.693IleLys: 1.693 ± 0.074
4.044IleLeu: 4.044 ± 0.112
1.131IleMet: 1.131 ± 0.048
1.674IleAsn: 1.674 ± 0.06
2.442IlePro: 2.442 ± 0.077
1.497IleGln: 1.497 ± 0.058
2.666IleArg: 2.666 ± 0.072
3.286IleSer: 3.286 ± 0.094
2.64IleThr: 2.64 ± 0.074
3.892IleVal: 3.892 ± 0.09
0.58IleTrp: 0.58 ± 0.036
1.121IleTyr: 1.121 ± 0.046
0.002IleXaa: 0.002 ± 0.002
Lys
4.872LysAla: 4.872 ± 0.114
0.118LysCys: 0.118 ± 0.018
2.383LysAsp: 2.383 ± 0.088
2.149LysGlu: 2.149 ± 0.069
0.81LysPhe: 0.81 ± 0.05
2.978LysGly: 2.978 ± 0.096
0.764LysHis: 0.764 ± 0.039
1.503LysIle: 1.503 ± 0.054
1.374LysLys: 1.374 ± 0.066
3.124LysLeu: 3.124 ± 0.086
0.849LysMet: 0.849 ± 0.046
1.036LysAsn: 1.036 ± 0.051
2.398LysPro: 2.398 ± 0.076
1.537LysGln: 1.537 ± 0.065
2.66LysArg: 2.66 ± 0.07
2.244LysSer: 2.244 ± 0.074
2.265LysThr: 2.265 ± 0.07
2.755LysVal: 2.755 ± 0.071
0.38LysTrp: 0.38 ± 0.022
0.92LysTyr: 0.92 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
11.474LeuAla: 11.474 ± 0.2
0.933LeuCys: 0.933 ± 0.044
6.155LeuAsp: 6.155 ± 0.141
4.8LeuGlu: 4.8 ± 0.126
2.909LeuPhe: 2.909 ± 0.099
8.109LeuGly: 8.109 ± 0.154
1.575LeuHis: 1.575 ± 0.057
4.669LeuIle: 4.669 ± 0.118
3.624LeuLys: 3.624 ± 0.092
8.84LeuLeu: 8.84 ± 0.168
2.128LeuMet: 2.128 ± 0.068
2.674LeuAsn: 2.674 ± 0.076
5.31LeuPro: 5.31 ± 0.113
2.657LeuGln: 2.657 ± 0.073
5.56LeuArg: 5.56 ± 0.122
6.448LeuSer: 6.448 ± 0.132
5.859LeuThr: 5.859 ± 0.118
7.134LeuVal: 7.134 ± 0.147
1.087LeuTrp: 1.087 ± 0.051
1.978LeuTyr: 1.978 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
2.828MetAla: 2.828 ± 0.079
0.188MetCys: 0.188 ± 0.018
1.61MetAsp: 1.61 ± 0.06
1.197MetGlu: 1.197 ± 0.048
0.684MetPhe: 0.684 ± 0.034
1.997MetGly: 1.997 ± 0.06
0.454MetHis: 0.454 ± 0.031
1.138MetIle: 1.138 ± 0.049
0.83MetLys: 0.83 ± 0.043
2.111MetLeu: 2.111 ± 0.079
0.612MetMet: 0.612 ± 0.042
0.867MetAsn: 0.867 ± 0.04
1.319MetPro: 1.319 ± 0.049
0.815MetGln: 0.815 ± 0.038
1.629MetArg: 1.629 ± 0.055
1.866MetSer: 1.866 ± 0.059
1.706MetThr: 1.706 ± 0.058
1.758MetVal: 1.758 ± 0.068
0.241MetTrp: 0.241 ± 0.02
0.46MetTyr: 0.46 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.86AsnAla: 2.86 ± 0.083
0.192AsnCys: 0.192 ± 0.02
1.562AsnAsp: 1.562 ± 0.056
1.477AsnGlu: 1.477 ± 0.052
0.77AsnPhe: 0.77 ± 0.041
2.605AsnGly: 2.605 ± 0.092
0.574AsnHis: 0.574 ± 0.04
1.26AsnIle: 1.26 ± 0.054
1.057AsnLys: 1.057 ± 0.054
2.508AsnLeu: 2.508 ± 0.078
0.726AsnMet: 0.726 ± 0.04
0.747AsnAsn: 0.747 ± 0.044
2.159AsnPro: 2.159 ± 0.065
1.26AsnGln: 1.26 ± 0.056
1.636AsnArg: 1.636 ± 0.056
1.553AsnSer: 1.553 ± 0.066
1.486AsnThr: 1.486 ± 0.079
1.754AsnVal: 1.754 ± 0.059
0.445AsnTrp: 0.445 ± 0.033
0.773AsnTyr: 0.773 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
5.959ProAla: 5.959 ± 0.157
0.323ProCys: 0.323 ± 0.028
3.588ProAsp: 3.588 ± 0.091
3.348ProGlu: 3.348 ± 0.083
1.505ProPhe: 1.505 ± 0.059
4.8ProGly: 4.8 ± 0.108
0.87ProHis: 0.87 ± 0.049
2.32ProIle: 2.32 ± 0.074
1.883ProLys: 1.883 ± 0.066
4.289ProLeu: 4.289 ± 0.104
0.986ProMet: 0.986 ± 0.048
1.387ProAsn: 1.387 ± 0.049
1.689ProPro: 1.689 ± 0.061
2.328ProGln: 2.328 ± 0.065
2.716ProArg: 2.716 ± 0.085
3.772ProSer: 3.772 ± 0.092
2.963ProThr: 2.963 ± 0.091
4.236ProVal: 4.236 ± 0.104
0.753ProTrp: 0.753 ± 0.04
1.38ProTyr: 1.38 ± 0.058
0.0ProXaa: 0.0 ± 0.0
Gln
6.634GlnAla: 6.634 ± 0.136
0.182GlnCys: 0.182 ± 0.019
2.322GlnAsp: 2.322 ± 0.074
2.801GlnGlu: 2.801 ± 0.093
0.924GlnPhe: 0.924 ± 0.043
4.044GlnGly: 4.044 ± 0.093
0.682GlnHis: 0.682 ± 0.032
1.898GlnIle: 1.898 ± 0.066
1.036GlnLys: 1.036 ± 0.047
3.656GlnLeu: 3.656 ± 0.085
1.022GlnMet: 1.022 ± 0.041
0.903GlnAsn: 0.903 ± 0.043
2.237GlnPro: 2.237 ± 0.079
1.8GlnGln: 1.8 ± 0.069
2.64GlnArg: 2.64 ± 0.077
2.503GlnSer: 2.503 ± 0.073
2.261GlnThr: 2.261 ± 0.076
3.867GlnVal: 3.867 ± 0.093
0.517GlnTrp: 0.517 ± 0.033
0.924GlnTyr: 0.924 ± 0.046
0.002GlnXaa: 0.002 ± 0.002
Arg
6.269ArgAla: 6.269 ± 0.132
0.481ArgCys: 0.481 ± 0.035
3.459ArgAsp: 3.459 ± 0.1
3.728ArgGlu: 3.728 ± 0.111
2.356ArgPhe: 2.356 ± 0.069
4.426ArgGly: 4.426 ± 0.1
1.342ArgHis: 1.342 ± 0.055
3.639ArgIle: 3.639 ± 0.095
2.63ArgLys: 2.63 ± 0.078
6.588ArgLeu: 6.588 ± 0.146
1.897ArgMet: 1.897 ± 0.056
1.625ArgAsn: 1.625 ± 0.054
3.293ArgPro: 3.293 ± 0.096
3.39ArgGln: 3.39 ± 0.094
5.302ArgArg: 5.302 ± 0.143
4.289ArgSer: 4.289 ± 0.103
3.438ArgThr: 3.438 ± 0.092
4.61ArgVal: 4.61 ± 0.105
0.933ArgTrp: 0.933 ± 0.044
1.868ArgTyr: 1.868 ± 0.066
0.0ArgXaa: 0.0 ± 0.0
Ser
7.303SerAla: 7.303 ± 0.127
0.507SerCys: 0.507 ± 0.038
4.029SerAsp: 4.029 ± 0.095
3.238SerGlu: 3.238 ± 0.085
2.206SerPhe: 2.206 ± 0.06
6.336SerGly: 6.336 ± 0.125
1.283SerHis: 1.283 ± 0.048
3.303SerIle: 3.303 ± 0.079
2.617SerLys: 2.617 ± 0.067
6.309SerLeu: 6.309 ± 0.117
1.714SerMet: 1.714 ± 0.06
1.87SerAsn: 1.87 ± 0.071
3.172SerPro: 3.172 ± 0.08
3.16SerGln: 3.16 ± 0.083
4.266SerArg: 4.266 ± 0.101
4.842SerSer: 4.842 ± 0.132
3.462SerThr: 3.462 ± 0.09
4.768SerVal: 4.768 ± 0.108
1.131SerTrp: 1.131 ± 0.051
1.784SerTyr: 1.784 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
5.589ThrAla: 5.589 ± 0.111
0.447ThrCys: 0.447 ± 0.026
3.196ThrAsp: 3.196 ± 0.09
2.17ThrGlu: 2.17 ± 0.071
1.765ThrPhe: 1.765 ± 0.075
5.118ThrGly: 5.118 ± 0.101
1.034ThrHis: 1.034 ± 0.047
2.972ThrIle: 2.972 ± 0.086
1.805ThrLys: 1.805 ± 0.054
5.138ThrLeu: 5.138 ± 0.114
1.245ThrMet: 1.245 ± 0.051
1.539ThrAsn: 1.539 ± 0.063
3.191ThrPro: 3.191 ± 0.095
2.096ThrGln: 2.096 ± 0.069
3.033ThrArg: 3.033 ± 0.088
3.282ThrSer: 3.282 ± 0.09
3.008ThrThr: 3.008 ± 0.109
4.43ThrVal: 4.43 ± 0.105
0.796ThrTrp: 0.796 ± 0.044
1.52ThrTyr: 1.52 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
7.736ValAla: 7.736 ± 0.133
0.893ValCys: 0.893 ± 0.044
5.078ValAsp: 5.078 ± 0.128
4.0ValGlu: 4.0 ± 0.092
2.668ValPhe: 2.668 ± 0.091
5.636ValGly: 5.636 ± 0.118
1.42ValHis: 1.42 ± 0.054
3.983ValIle: 3.983 ± 0.087
2.809ValLys: 2.809 ± 0.087
6.752ValLeu: 6.752 ± 0.15
1.693ValMet: 1.693 ± 0.052
2.201ValAsn: 2.201 ± 0.063
3.527ValPro: 3.527 ± 0.079
2.442ValGln: 2.442 ± 0.069
4.468ValArg: 4.468 ± 0.1
5.507ValSer: 5.507 ± 0.114
4.217ValThr: 4.217 ± 0.082
5.859ValVal: 5.859 ± 0.136
0.918ValTrp: 0.918 ± 0.04
1.754ValTyr: 1.754 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
1.378TrpAla: 1.378 ± 0.052
0.141TrpCys: 0.141 ± 0.017
0.735TrpAsp: 0.735 ± 0.045
0.564TrpGlu: 0.564 ± 0.033
0.504TrpPhe: 0.504 ± 0.038
1.129TrpGly: 1.129 ± 0.071
0.298TrpHis: 0.298 ± 0.025
0.675TrpIle: 0.675 ± 0.038
0.555TrpLys: 0.555 ± 0.035
1.478TrpLeu: 1.478 ± 0.06
0.388TrpMet: 0.388 ± 0.024
0.49TrpAsn: 0.49 ± 0.03
0.589TrpPro: 0.589 ± 0.042
0.703TrpGln: 0.703 ± 0.039
1.089TrpArg: 1.089 ± 0.055
1.022TrpSer: 1.022 ± 0.04
0.781TrpThr: 0.781 ± 0.048
0.925TrpVal: 0.925 ± 0.048
0.258TrpTrp: 0.258 ± 0.022
0.365TrpTyr: 0.365 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.693TyrAla: 2.693 ± 0.075
0.262TyrCys: 0.262 ± 0.023
1.573TyrAsp: 1.573 ± 0.059
1.511TyrGlu: 1.511 ± 0.063
0.878TyrPhe: 0.878 ± 0.039
2.391TyrGly: 2.391 ± 0.069
0.534TyrHis: 0.534 ± 0.035
1.114TyrIle: 1.114 ± 0.045
0.838TyrLys: 0.838 ± 0.048
2.469TyrLeu: 2.469 ± 0.077
0.593TyrMet: 0.593 ± 0.036
0.817TyrAsn: 0.817 ± 0.046
1.262TyrPro: 1.262 ± 0.057
1.188TyrGln: 1.188 ± 0.053
1.802TyrArg: 1.802 ± 0.07
1.627TyrSer: 1.627 ± 0.062
1.402TyrThr: 1.402 ± 0.058
1.657TyrVal: 1.657 ± 0.056
0.407TyrTrp: 0.407 ± 0.03
0.84TyrTyr: 0.84 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.002XaaGly: 0.002 ± 0.002
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.002XaaPro: 0.002 ± 0.002
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1484 proteins (526225 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski