Amino acid dipepetide frequency for Pedobacter sp. KBW01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.642AlaAla: 6.642 ± 0.076
0.659AlaCys: 0.659 ± 0.023
4.573AlaAsp: 4.573 ± 0.055
4.537AlaGlu: 4.537 ± 0.066
3.677AlaPhe: 3.677 ± 0.047
5.765AlaGly: 5.765 ± 0.09
1.194AlaHis: 1.194 ± 0.028
5.508AlaIle: 5.508 ± 0.068
5.466AlaLys: 5.466 ± 0.065
7.164AlaLeu: 7.164 ± 0.067
1.612AlaMet: 1.612 ± 0.032
4.383AlaAsn: 4.383 ± 0.069
2.425AlaPro: 2.425 ± 0.042
3.049AlaGln: 3.049 ± 0.044
2.36AlaArg: 2.36 ± 0.037
4.76AlaSer: 4.76 ± 0.059
4.678AlaThr: 4.678 ± 0.149
4.925AlaVal: 4.925 ± 0.065
0.802AlaTrp: 0.802 ± 0.023
3.113AlaTyr: 3.113 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.523CysAla: 0.523 ± 0.019
0.121CysCys: 0.121 ± 0.009
0.344CysAsp: 0.344 ± 0.013
0.342CysGlu: 0.342 ± 0.016
0.413CysPhe: 0.413 ± 0.014
0.568CysGly: 0.568 ± 0.018
0.182CysHis: 0.182 ± 0.011
0.579CysIle: 0.579 ± 0.017
0.539CysLys: 0.539 ± 0.017
0.767CysLeu: 0.767 ± 0.021
0.158CysMet: 0.158 ± 0.009
0.392CysAsn: 0.392 ± 0.015
0.279CysPro: 0.279 ± 0.014
0.215CysGln: 0.215 ± 0.011
0.293CysArg: 0.293 ± 0.013
0.476CysSer: 0.476 ± 0.017
0.423CysThr: 0.423 ± 0.016
0.415CysVal: 0.415 ± 0.016
0.08CysTrp: 0.08 ± 0.007
0.304CysTyr: 0.304 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
4.17AspAla: 4.17 ± 0.064
0.378AspCys: 0.378 ± 0.013
2.5AspAsp: 2.5 ± 0.045
3.24AspGlu: 3.24 ± 0.047
3.203AspPhe: 3.203 ± 0.045
4.02AspGly: 4.02 ± 0.074
0.96AspHis: 0.96 ± 0.023
3.804AspIle: 3.804 ± 0.042
3.989AspLys: 3.989 ± 0.058
4.946AspLeu: 4.946 ± 0.062
1.135AspMet: 1.135 ± 0.025
2.77AspAsn: 2.77 ± 0.047
1.978AspPro: 1.978 ± 0.035
1.935AspGln: 1.935 ± 0.031
2.011AspArg: 2.011 ± 0.031
2.72AspSer: 2.72 ± 0.038
2.447AspThr: 2.447 ± 0.042
3.358AspVal: 3.358 ± 0.046
0.787AspTrp: 0.787 ± 0.025
2.596AspTyr: 2.596 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
4.027GluAla: 4.027 ± 0.056
0.292GluCys: 0.292 ± 0.014
2.504GluAsp: 2.504 ± 0.043
3.293GluGlu: 3.293 ± 0.059
2.255GluPhe: 2.255 ± 0.04
3.134GluGly: 3.134 ± 0.048
0.995GluHis: 0.995 ± 0.027
4.459GluIle: 4.459 ± 0.072
4.745GluLys: 4.745 ± 0.067
5.278GluLeu: 5.278 ± 0.077
1.39GluMet: 1.39 ± 0.028
3.496GluAsn: 3.496 ± 0.046
1.505GluPro: 1.505 ± 0.033
2.28GluGln: 2.28 ± 0.039
2.311GluArg: 2.311 ± 0.043
2.799GluSer: 2.799 ± 0.039
3.02GluThr: 3.02 ± 0.043
3.748GluVal: 3.748 ± 0.051
0.606GluTrp: 0.606 ± 0.017
1.89GluTyr: 1.89 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
3.504PheAla: 3.504 ± 0.046
0.478PheCys: 0.478 ± 0.018
2.992PheAsp: 2.992 ± 0.039
2.782PheGlu: 2.782 ± 0.043
2.471PhePhe: 2.471 ± 0.052
3.479PheGly: 3.479 ± 0.052
0.721PheHis: 0.721 ± 0.02
3.491PheIle: 3.491 ± 0.054
3.539PheLys: 3.539 ± 0.052
4.314PheLeu: 4.314 ± 0.073
1.039PheMet: 1.039 ± 0.027
3.369PheAsn: 3.369 ± 0.049
1.701PhePro: 1.701 ± 0.034
1.383PheGln: 1.383 ± 0.028
1.663PheArg: 1.663 ± 0.033
3.842PheSer: 3.842 ± 0.052
3.233PheThr: 3.233 ± 0.056
2.823PheVal: 2.823 ± 0.041
0.613PheTrp: 0.613 ± 0.02
2.258PheTyr: 2.258 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.864GlyAla: 4.864 ± 0.064
0.573GlyCys: 0.573 ± 0.02
3.393GlyAsp: 3.393 ± 0.046
3.309GlyGlu: 3.309 ± 0.043
3.679GlyPhe: 3.679 ± 0.053
5.03GlyGly: 5.03 ± 0.073
1.091GlyHis: 1.091 ± 0.026
5.357GlyIle: 5.357 ± 0.057
5.566GlyLys: 5.566 ± 0.059
6.417GlyLeu: 6.417 ± 0.066
1.563GlyMet: 1.563 ± 0.034
4.111GlyAsn: 4.111 ± 0.067
1.564GlyPro: 1.564 ± 0.033
2.297GlyGln: 2.297 ± 0.041
2.408GlyArg: 2.408 ± 0.042
4.539GlySer: 4.539 ± 0.065
4.674GlyThr: 4.674 ± 0.142
4.313GlyVal: 4.313 ± 0.056
0.975GlyTrp: 0.975 ± 0.027
3.296GlyTyr: 3.296 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
1.105HisAla: 1.105 ± 0.025
0.163HisCys: 0.163 ± 0.009
0.774HisAsp: 0.774 ± 0.02
0.869HisGlu: 0.869 ± 0.025
1.086HisPhe: 1.086 ± 0.026
1.045HisGly: 1.045 ± 0.028
0.454HisHis: 0.454 ± 0.018
1.228HisIle: 1.228 ± 0.033
0.986HisLys: 0.986 ± 0.027
1.684HisLeu: 1.684 ± 0.037
0.31HisMet: 0.31 ± 0.012
0.911HisAsn: 0.911 ± 0.023
0.885HisPro: 0.885 ± 0.026
0.767HisGln: 0.767 ± 0.022
0.659HisArg: 0.659 ± 0.017
0.936HisSer: 0.936 ± 0.023
0.922HisThr: 0.922 ± 0.019
0.857HisVal: 0.857 ± 0.023
0.228HisTrp: 0.228 ± 0.011
0.804HisTyr: 0.804 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.873IleAla: 5.873 ± 0.071
0.687IleCys: 0.687 ± 0.021
4.241IleAsp: 4.241 ± 0.052
4.065IleGlu: 4.065 ± 0.059
3.217IlePhe: 3.217 ± 0.05
4.841IleGly: 4.841 ± 0.055
1.095IleHis: 1.095 ± 0.024
4.895IleIle: 4.895 ± 0.066
5.394IleLys: 5.394 ± 0.072
6.166IleLeu: 6.166 ± 0.072
1.293IleMet: 1.293 ± 0.031
4.629IleAsn: 4.629 ± 0.06
2.948IlePro: 2.948 ± 0.048
2.267IleGln: 2.267 ± 0.035
2.692IleArg: 2.692 ± 0.043
5.3IleSer: 5.3 ± 0.056
4.682IleThr: 4.682 ± 0.085
4.175IleVal: 4.175 ± 0.049
0.738IleTrp: 0.738 ± 0.02
2.703IleTyr: 2.703 ± 0.04
0.0IleXaa: 0.0 ± 0.0
Lys
5.765LysAla: 5.765 ± 0.069
0.304LysCys: 0.304 ± 0.016
4.199LysAsp: 4.199 ± 0.052
4.329LysGlu: 4.329 ± 0.06
2.822LysPhe: 2.822 ± 0.04
4.75LysGly: 4.75 ± 0.046
1.238LysHis: 1.238 ± 0.028
5.482LysIle: 5.482 ± 0.063
5.772LysLys: 5.772 ± 0.071
6.752LysLeu: 6.752 ± 0.068
1.885LysMet: 1.885 ± 0.035
4.805LysAsn: 4.805 ± 0.048
2.917LysPro: 2.917 ± 0.042
2.947LysGln: 2.947 ± 0.047
2.653LysArg: 2.653 ± 0.038
4.199LysSer: 4.199 ± 0.056
4.726LysThr: 4.726 ± 0.049
4.564LysVal: 4.564 ± 0.064
0.864LysTrp: 0.864 ± 0.024
3.04LysTyr: 3.04 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
7.231LeuAla: 7.231 ± 0.072
0.741LeuCys: 0.741 ± 0.023
4.672LeuAsp: 4.672 ± 0.058
4.506LeuGlu: 4.506 ± 0.065
4.635LeuPhe: 4.635 ± 0.068
5.786LeuGly: 5.786 ± 0.062
1.471LeuHis: 1.471 ± 0.03
6.598LeuIle: 6.598 ± 0.079
7.719LeuLys: 7.719 ± 0.088
8.952LeuLeu: 8.952 ± 0.105
2.064LeuMet: 2.064 ± 0.03
6.128LeuAsn: 6.128 ± 0.063
3.99LeuPro: 3.99 ± 0.05
3.486LeuGln: 3.486 ± 0.053
3.348LeuArg: 3.348 ± 0.048
7.072LeuSer: 7.072 ± 0.069
5.766LeuThr: 5.766 ± 0.085
5.436LeuVal: 5.436 ± 0.058
0.93LeuTrp: 0.93 ± 0.025
3.303LeuTyr: 3.303 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
1.792MetAla: 1.792 ± 0.033
0.137MetCys: 0.137 ± 0.009
1.139MetAsp: 1.139 ± 0.029
1.203MetGlu: 1.203 ± 0.027
0.846MetPhe: 0.846 ± 0.023
1.478MetGly: 1.478 ± 0.031
0.391MetHis: 0.391 ± 0.014
1.339MetIle: 1.339 ± 0.03
1.882MetLys: 1.882 ± 0.033
2.004MetLeu: 2.004 ± 0.036
0.555MetMet: 0.555 ± 0.018
1.197MetAsn: 1.197 ± 0.026
1.025MetPro: 1.025 ± 0.027
0.95MetGln: 0.95 ± 0.027
0.86MetArg: 0.86 ± 0.024
1.234MetSer: 1.234 ± 0.024
0.939MetThr: 0.939 ± 0.026
1.455MetVal: 1.455 ± 0.028
0.183MetTrp: 0.183 ± 0.011
0.644MetTyr: 0.644 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
4.513AsnAla: 4.513 ± 0.05
0.419AsnCys: 0.419 ± 0.017
2.938AsnAsp: 2.938 ± 0.051
3.086AsnGlu: 3.086 ± 0.044
2.999AsnPhe: 2.999 ± 0.044
4.736AsnGly: 4.736 ± 0.077
1.037AsnHis: 1.037 ± 0.024
4.463AsnIle: 4.463 ± 0.056
4.014AsnLys: 4.014 ± 0.051
5.625AsnLeu: 5.625 ± 0.064
1.228AsnMet: 1.228 ± 0.025
3.827AsnAsn: 3.827 ± 0.056
2.906AsnPro: 2.906 ± 0.047
2.436AsnGln: 2.436 ± 0.039
2.333AsnArg: 2.333 ± 0.034
3.555AsnSer: 3.555 ± 0.054
3.784AsnThr: 3.784 ± 0.058
3.415AsnVal: 3.415 ± 0.05
0.851AsnTrp: 0.851 ± 0.019
2.951AsnTyr: 2.951 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
3.33ProAla: 3.33 ± 0.076
0.216ProCys: 0.216 ± 0.011
2.36ProAsp: 2.36 ± 0.035
2.599ProGlu: 2.599 ± 0.043
1.927ProPhe: 1.927 ± 0.032
2.673ProGly: 2.673 ± 0.036
0.605ProHis: 0.605 ± 0.017
2.362ProIle: 2.362 ± 0.039
2.466ProLys: 2.466 ± 0.037
3.231ProLeu: 3.231 ± 0.041
0.753ProMet: 0.753 ± 0.02
2.214ProAsn: 2.214 ± 0.034
0.925ProPro: 0.925 ± 0.024
1.298ProGln: 1.298 ± 0.028
1.002ProArg: 1.002 ± 0.025
2.106ProSer: 2.106 ± 0.044
2.139ProThr: 2.139 ± 0.038
3.085ProVal: 3.085 ± 0.057
0.409ProTrp: 0.409 ± 0.016
1.499ProTyr: 1.499 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
2.766GlnAla: 2.766 ± 0.042
0.172GlnCys: 0.172 ± 0.01
1.644GlnAsp: 1.644 ± 0.033
1.888GlnGlu: 1.888 ± 0.029
1.667GlnPhe: 1.667 ± 0.03
2.09GlnGly: 2.09 ± 0.03
0.713GlnHis: 0.713 ± 0.019
2.613GlnIle: 2.613 ± 0.033
2.769GlnLys: 2.769 ± 0.04
3.804GlnLeu: 3.804 ± 0.051
0.832GlnMet: 0.832 ± 0.023
2.369GlnAsn: 2.369 ± 0.04
1.397GlnPro: 1.397 ± 0.029
1.944GlnGln: 1.944 ± 0.049
1.367GlnArg: 1.367 ± 0.03
2.246GlnSer: 2.246 ± 0.036
2.141GlnThr: 2.141 ± 0.035
2.311GlnVal: 2.311 ± 0.039
0.43GlnTrp: 0.43 ± 0.015
1.555GlnTyr: 1.555 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
2.343ArgAla: 2.343 ± 0.041
0.23ArgCys: 0.23 ± 0.012
1.848ArgAsp: 1.848 ± 0.031
2.003ArgGlu: 2.003 ± 0.039
2.067ArgPhe: 2.067 ± 0.038
2.057ArgGly: 2.057 ± 0.037
0.554ArgHis: 0.554 ± 0.017
2.808ArgIle: 2.808 ± 0.045
2.711ArgLys: 2.711 ± 0.046
3.641ArgLeu: 3.641 ± 0.049
0.919ArgMet: 0.919 ± 0.023
2.232ArgAsn: 2.232 ± 0.036
1.227ArgPro: 1.227 ± 0.026
1.236ArgGln: 1.236 ± 0.024
1.387ArgArg: 1.387 ± 0.028
2.101ArgSer: 2.101 ± 0.04
2.059ArgThr: 2.059 ± 0.038
2.178ArgVal: 2.178 ± 0.032
0.503ArgTrp: 0.503 ± 0.013
1.823ArgTyr: 1.823 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
5.04SerAla: 5.04 ± 0.066
0.539SerCys: 0.539 ± 0.018
3.125SerAsp: 3.125 ± 0.049
2.911SerGlu: 2.911 ± 0.044
3.66SerPhe: 3.66 ± 0.052
5.1SerGly: 5.1 ± 0.065
1.013SerHis: 1.013 ± 0.028
4.728SerIle: 4.728 ± 0.059
4.144SerLys: 4.144 ± 0.046
6.143SerLeu: 6.143 ± 0.065
1.2SerMet: 1.2 ± 0.027
3.489SerAsn: 3.489 ± 0.048
2.403SerPro: 2.403 ± 0.038
1.947SerGln: 1.947 ± 0.036
2.325SerArg: 2.325 ± 0.038
4.09SerSer: 4.09 ± 0.057
3.823SerThr: 3.823 ± 0.059
4.191SerVal: 4.191 ± 0.054
0.826SerTrp: 0.826 ± 0.025
2.857SerTyr: 2.857 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
5.236ThrAla: 5.236 ± 0.132
0.351ThrCys: 0.351 ± 0.014
3.551ThrAsp: 3.551 ± 0.052
3.183ThrGlu: 3.183 ± 0.048
2.982ThrPhe: 2.982 ± 0.057
4.919ThrGly: 4.919 ± 0.09
0.908ThrHis: 0.908 ± 0.025
4.405ThrIle: 4.405 ± 0.099
3.674ThrLys: 3.674 ± 0.045
5.795ThrLeu: 5.795 ± 0.098
0.934ThrMet: 0.934 ± 0.023
3.243ThrAsn: 3.243 ± 0.059
2.65ThrPro: 2.65 ± 0.06
1.934ThrGln: 1.934 ± 0.032
1.841ThrArg: 1.841 ± 0.035
3.69ThrSer: 3.69 ± 0.067
3.86ThrThr: 3.86 ± 0.101
4.186ThrVal: 4.186 ± 0.125
0.697ThrTrp: 0.697 ± 0.019
2.589ThrTyr: 2.589 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
4.738ValAla: 4.738 ± 0.075
0.487ValCys: 0.487 ± 0.017
3.251ValAsp: 3.251 ± 0.049
3.31ValGlu: 3.31 ± 0.05
3.128ValPhe: 3.128 ± 0.051
3.787ValGly: 3.787 ± 0.059
0.964ValHis: 0.964 ± 0.026
4.486ValIle: 4.486 ± 0.051
4.881ValLys: 4.881 ± 0.057
5.943ValLeu: 5.943 ± 0.062
1.313ValMet: 1.313 ± 0.028
3.995ValAsn: 3.995 ± 0.063
2.31ValPro: 2.31 ± 0.038
1.986ValGln: 1.986 ± 0.036
2.141ValArg: 2.141 ± 0.037
4.416ValSer: 4.416 ± 0.061
3.959ValThr: 3.959 ± 0.122
4.207ValVal: 4.207 ± 0.058
0.705ValTrp: 0.705 ± 0.021
2.541ValTyr: 2.541 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
0.812TrpAla: 0.812 ± 0.023
0.133TrpCys: 0.133 ± 0.008
0.637TrpAsp: 0.637 ± 0.019
0.68TrpGlu: 0.68 ± 0.019
0.591TrpPhe: 0.591 ± 0.019
0.863TrpGly: 0.863 ± 0.023
0.254TrpHis: 0.254 ± 0.013
0.747TrpIle: 0.747 ± 0.022
0.877TrpLys: 0.877 ± 0.022
1.188TrpLeu: 1.188 ± 0.028
0.327TrpMet: 0.327 ± 0.014
0.726TrpAsn: 0.726 ± 0.023
0.393TrpPro: 0.393 ± 0.016
0.529TrpGln: 0.529 ± 0.013
0.464TrpArg: 0.464 ± 0.016
0.694TrpSer: 0.694 ± 0.021
0.644TrpThr: 0.644 ± 0.019
0.71TrpVal: 0.71 ± 0.021
0.197TrpTrp: 0.197 ± 0.01
0.519TrpTyr: 0.519 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.018TyrAla: 3.018 ± 0.052
0.332TyrCys: 0.332 ± 0.013
2.262TyrAsp: 2.262 ± 0.043
1.889TyrGlu: 1.889 ± 0.031
2.371TyrPhe: 2.371 ± 0.039
2.899TyrGly: 2.899 ± 0.051
0.832TyrHis: 0.832 ± 0.023
2.547TyrIle: 2.547 ± 0.041
2.878TyrLys: 2.878 ± 0.039
4.019TyrLeu: 4.019 ± 0.058
0.742TyrMet: 0.742 ± 0.019
2.832TyrAsn: 2.832 ± 0.048
1.773TyrPro: 1.773 ± 0.034
1.833TyrGln: 1.833 ± 0.031
1.808TyrArg: 1.808 ± 0.034
2.778TyrSer: 2.778 ± 0.048
2.713TyrThr: 2.713 ± 0.059
2.195TyrVal: 2.195 ± 0.04
0.563TyrTrp: 0.563 ± 0.019
1.969TyrTyr: 1.969 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5297 proteins (1900158 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski