Staphylococcus phage phiSP38-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Peeveelvirus; unclassified Peeveelvirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A499SHM7|A0A499SHM7_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSP38-1 OX=2491320 PE=4 SV=1
MM1 pKa = 7.4MIKK4 pKa = 9.74IYY6 pKa = 10.8KK7 pKa = 9.61NEE9 pKa = 3.86NDD11 pKa = 3.52EE12 pKa = 4.82LEE14 pKa = 4.32CHH16 pKa = 6.0VNYY19 pKa = 10.56AGYY22 pKa = 10.33DD23 pKa = 3.51FKK25 pKa = 11.25FQCIKK30 pKa = 10.5NGSGATFKK38 pKa = 10.96GSNSTEE44 pKa = 3.78YY45 pKa = 11.27LEE47 pKa = 4.27FEE49 pKa = 5.06SYY51 pKa = 10.64IDD53 pKa = 4.56DD54 pKa = 5.12DD55 pKa = 6.26GEE57 pKa = 4.63ILDD60 pKa = 4.08NLQDD64 pKa = 3.63VMYY67 pKa = 10.21HH68 pKa = 6.17IASVCNWRR76 pKa = 11.84EE77 pKa = 3.91SFGEE81 pKa = 4.02EE82 pKa = 3.58RR83 pKa = 4.2

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A499SIS9|A0A499SIS9_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSP38-1 OX=2491320 PE=3 SV=1
MM1 pKa = 6.63TWKK4 pKa = 10.66DD5 pKa = 2.72IDD7 pKa = 4.33FIGLTKK13 pKa = 10.23SQKK16 pKa = 11.11AKK18 pKa = 9.69MIHH21 pKa = 6.36KK22 pKa = 10.39GITPSIALSRR32 pKa = 11.84YY33 pKa = 7.76KK34 pKa = 10.57NYY36 pKa = 10.04WSIEE40 pKa = 4.17EE41 pKa = 4.14IVNTKK46 pKa = 10.31PYY48 pKa = 8.75MKK50 pKa = 10.02RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84KK54 pKa = 9.6

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13544

45

1480

185.5

21.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.567 ± 0.379

0.48 ± 0.107

6.638 ± 0.303

7.716 ± 0.551

4.024 ± 0.207

5.626 ± 0.51

1.61 ± 0.137

7.324 ± 0.251

9.089 ± 0.353

8.033 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.182

6.305 ± 0.295

2.643 ± 0.15

3.721 ± 0.185

4.755 ± 0.245

6.143 ± 0.311

6.202 ± 0.249

6.195 ± 0.287

1.056 ± 0.141

4.209 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski