Lysobacter concretionis Ko07 = DSM 16239

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; Lysobacter concretionis

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2232 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0ERD0|A0A0A0ERD0_9GAMM Pyridoxine 5'-phosphate synthase OS=Lysobacter concretionis Ko07 = DSM 16239 OX=1122185 GN=pdxJ PE=3 SV=1
MM1 pKa = 7.16VCLLSSLLLAVLLAACGGDD20 pKa = 3.89GDD22 pKa = 4.49TGPAATSSTAAMPEE36 pKa = 4.11PQQGQYY42 pKa = 10.76SDD44 pKa = 4.41QPDD47 pKa = 3.76EE48 pKa = 4.69DD49 pKa = 4.46VPPATAPPGSLASADD64 pKa = 4.12LSADD68 pKa = 3.63PPDD71 pKa = 4.8PLPGDD76 pKa = 3.62AHH78 pKa = 6.75FDD80 pKa = 3.75GYY82 pKa = 9.36GQAMFGMDD90 pKa = 3.82ADD92 pKa = 3.94QVRR95 pKa = 11.84SVWVGGLDD103 pKa = 3.41GNAAEE108 pKa = 5.41GEE110 pKa = 4.55ACFHH114 pKa = 6.95LSPAGQPVIADD125 pKa = 4.24FALMFEE131 pKa = 4.07NGKK134 pKa = 9.08FVRR137 pKa = 11.84YY138 pKa = 9.48SVASDD143 pKa = 3.43ALVAPGGGRR152 pKa = 11.84RR153 pKa = 11.84GMDD156 pKa = 2.76IAQIQQLYY164 pKa = 8.52PGRR167 pKa = 11.84VVQSPHH173 pKa = 6.66KK174 pKa = 8.79YY175 pKa = 10.09VEE177 pKa = 4.4GGHH180 pKa = 5.84YY181 pKa = 10.46LRR183 pKa = 11.84IPDD186 pKa = 4.5DD187 pKa = 4.17GGSNGVLVFEE197 pKa = 4.7TDD199 pKa = 2.88ADD201 pKa = 4.02GTVTEE206 pKa = 4.59WRR208 pKa = 11.84VGVPPQVDD216 pKa = 3.78YY217 pKa = 12.02VEE219 pKa = 5.01GCSS222 pKa = 3.53

Molecular weight: 22.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0ER27|A0A0A0ER27_9GAMM Uncharacterized protein OS=Lysobacter concretionis Ko07 = DSM 16239 OX=1122185 GN=N792_08115 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 3.87PHH4 pKa = 6.48GAGVHH9 pKa = 5.95ALCIALANGDD19 pKa = 3.55VDD21 pKa = 3.87RR22 pKa = 11.84ALALGLLKK30 pKa = 10.91AMPCPACSVEE40 pKa = 4.21CQVALVQARR49 pKa = 11.84VEE51 pKa = 4.31RR52 pKa = 11.84KK53 pKa = 8.28HH54 pKa = 6.86ALAARR59 pKa = 11.84EE60 pKa = 3.97RR61 pKa = 11.84YY62 pKa = 8.82RR63 pKa = 11.84ARR65 pKa = 11.84NARR68 pKa = 11.84LQRR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 6.45DD74 pKa = 3.49EE75 pKa = 3.83RR76 pKa = 11.84ATRR79 pKa = 11.84RR80 pKa = 11.84GVTTSRR86 pKa = 11.84PEE88 pKa = 4.17DD89 pKa = 3.74PTAGPPTNPPAPDD102 pKa = 3.44PTNRR106 pKa = 11.84TPRR109 pKa = 11.84PALPAAVAAALARR122 pKa = 11.84AKK124 pKa = 10.39AKK126 pKa = 10.35AAATPPPAGPEE137 pKa = 4.04SS138 pKa = 3.55

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2232

0

2232

740093

41

1671

331.6

36.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.804 ± 0.072

0.788 ± 0.016

6.137 ± 0.041

5.49 ± 0.048

3.379 ± 0.034

8.677 ± 0.046

2.29 ± 0.026

4.386 ± 0.035

2.757 ± 0.044

10.717 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.024

2.457 ± 0.028

5.304 ± 0.043

3.648 ± 0.029

7.566 ± 0.052

5.116 ± 0.04

4.923 ± 0.031

7.55 ± 0.048

1.463 ± 0.021

2.253 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski