Zoo ranavirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Frog virus 3

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8UGY8|A0A3Q8UGY8_FRG3V DNA-directed RNA polymerase subunit beta OS=Zoo ranavirus OX=1419340 GN=ORF061 PE=3 SV=1
MM1 pKa = 7.31FALEE5 pKa = 4.36TYY7 pKa = 9.33KK8 pKa = 10.87CKK10 pKa = 10.52NSSMSFKK17 pKa = 10.59RR18 pKa = 11.84IMDD21 pKa = 3.65ATDD24 pKa = 2.87IFGDD28 pKa = 3.35ISDD31 pKa = 4.37IGSASSGDD39 pKa = 3.59EE40 pKa = 3.86AEE42 pKa = 4.69FEE44 pKa = 3.91HH45 pKa = 7.49DD46 pKa = 4.07EE47 pKa = 4.19NVEE50 pKa = 3.88RR51 pKa = 11.84AGYY54 pKa = 9.34EE55 pKa = 4.02EE56 pKa = 5.49PDD58 pKa = 4.08DD59 pKa = 6.08DD60 pKa = 7.02GDD62 pKa = 5.34DD63 pKa = 4.16GDD65 pKa = 5.86DD66 pKa = 4.36GVDD69 pKa = 3.29SVNQAEE75 pKa = 4.31EE76 pKa = 3.46EE77 pKa = 4.02AAAVRR82 pKa = 11.84EE83 pKa = 4.31APSVVMPLDD92 pKa = 3.45MEE94 pKa = 4.76SYY96 pKa = 10.71SDD98 pKa = 3.96EE99 pKa = 4.32EE100 pKa = 4.31LAMMPLGAFASRR112 pKa = 11.84LTQMTAIRR120 pKa = 11.84DD121 pKa = 3.68ALLSDD126 pKa = 4.12FRR128 pKa = 11.84FTGDD132 pKa = 3.7LVTVSDD138 pKa = 3.7KK139 pKa = 10.37YY140 pKa = 11.55LRR142 pKa = 11.84MVAILILNNRR152 pKa = 11.84DD153 pKa = 3.7SLPNPYY159 pKa = 10.1AAYY162 pKa = 10.84NLLQYY167 pKa = 10.65ASKK170 pKa = 10.54RR171 pKa = 11.84GLVNPEE177 pKa = 3.56QAA179 pKa = 3.88

Molecular weight: 19.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8UGT8|A0A3Q8UGT8_FRG3V Cytosine DNA methyltransferase OS=Zoo ranavirus OX=1419340 GN=ORF082 PE=4 SV=1
MM1 pKa = 7.11YY2 pKa = 10.24SVRR5 pKa = 11.84NSGCSVGCSPRR16 pKa = 11.84QGASPIMFGPSLGAMLSAPVVRR38 pKa = 11.84ASSPVVKK45 pKa = 10.55ASSPVVKK52 pKa = 10.43ALSPVVKK59 pKa = 10.29ALSPVRR65 pKa = 11.84KK66 pKa = 9.53RR67 pKa = 11.84KK68 pKa = 10.2SLVKK72 pKa = 10.32RR73 pKa = 11.84KK74 pKa = 10.3SPVKK78 pKa = 10.27KK79 pKa = 10.51SPLKK83 pKa = 10.31KK84 pKa = 9.97RR85 pKa = 11.84SPLRR89 pKa = 11.84TSPVKK94 pKa = 10.44RR95 pKa = 11.84KK96 pKa = 10.42SPVKK100 pKa = 10.07RR101 pKa = 11.84KK102 pKa = 10.4SPVKK106 pKa = 10.07RR107 pKa = 11.84KK108 pKa = 10.4SPVKK112 pKa = 10.07RR113 pKa = 11.84KK114 pKa = 10.4SPVKK118 pKa = 10.07RR119 pKa = 11.84KK120 pKa = 10.4SPVKK124 pKa = 10.07RR125 pKa = 11.84KK126 pKa = 10.4SPVKK130 pKa = 10.07RR131 pKa = 11.84KK132 pKa = 10.4SPVKK136 pKa = 10.07RR137 pKa = 11.84KK138 pKa = 10.4SPVKK142 pKa = 10.07RR143 pKa = 11.84KK144 pKa = 10.4SPVKK148 pKa = 10.23RR149 pKa = 11.84KK150 pKa = 10.33SPVKK154 pKa = 10.02KK155 pKa = 10.07RR156 pKa = 11.84SPVKK160 pKa = 10.15RR161 pKa = 11.84KK162 pKa = 9.96SPAKK166 pKa = 9.96RR167 pKa = 11.84RR168 pKa = 11.84PQVCFMATKK177 pKa = 10.43

Molecular weight: 19.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

27902

34

1292

293.7

32.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.971 ± 0.275

2.175 ± 0.167

5.971 ± 0.18

5.544 ± 0.23

3.344 ± 0.11

7.394 ± 0.264

2.161 ± 0.137

3.347 ± 0.139

5.652 ± 0.241

8.175 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.0 ± 0.121

2.674 ± 0.128

6.157 ± 0.325

2.867 ± 0.2

7.118 ± 0.296

7.1 ± 0.207

5.419 ± 0.201

8.473 ± 0.222

1.441 ± 0.103

3.018 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski