Tortoise microvirus 32

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W9L2|A0A4P8W9L2_9VIRU DNA pilot protein OS=Tortoise microvirus 32 OX=2583135 PE=4 SV=1
MM1 pKa = 6.98NNKK4 pKa = 8.16WRR6 pKa = 11.84TPYY9 pKa = 10.46NSKK12 pKa = 10.31EE13 pKa = 3.82FPKK16 pKa = 10.45NYY18 pKa = 9.7EE19 pKa = 4.01VNTQPSQTIPDD30 pKa = 3.35QTMSVRR36 pKa = 11.84EE37 pKa = 3.76IMDD40 pKa = 3.86RR41 pKa = 11.84YY42 pKa = 10.95ARR44 pKa = 11.84GLPISAGKK52 pKa = 9.89VPIYY56 pKa = 10.87DD57 pKa = 3.83GDD59 pKa = 3.79EE60 pKa = 4.27DD61 pKa = 5.42LPDD64 pKa = 4.31FKK66 pKa = 11.54KK67 pKa = 10.07MDD69 pKa = 3.73LSEE72 pKa = 4.07QQEE75 pKa = 4.32LLRR78 pKa = 11.84ANAEE82 pKa = 4.17HH83 pKa = 7.39IEE85 pKa = 4.04RR86 pKa = 11.84LQSDD90 pKa = 4.11LSRR93 pKa = 11.84PKK95 pKa = 10.38EE96 pKa = 4.37SAKK99 pKa = 9.17HH100 pKa = 4.41TQPPTTEE107 pKa = 4.52EE108 pKa = 4.13SDD110 pKa = 3.78SPAPSAGQQ118 pKa = 3.1

Molecular weight: 13.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9F5|A0A4P8W9F5_9VIRU Uncharacterized protein OS=Tortoise microvirus 32 OX=2583135 PE=4 SV=1
MM1 pKa = 7.65ASCLTPYY8 pKa = 9.73IVKK11 pKa = 10.52HH12 pKa = 6.24NGDD15 pKa = 4.27EE16 pKa = 4.29QPVPCGKK23 pKa = 10.35CPACMKK29 pKa = 10.23RR30 pKa = 11.84RR31 pKa = 11.84ISGWSFRR38 pKa = 11.84LMQEE42 pKa = 3.98YY43 pKa = 10.0KK44 pKa = 9.68RR45 pKa = 11.84CHH47 pKa = 5.4SAHH50 pKa = 7.32FITLTYY56 pKa = 8.51DD57 pKa = 3.42TKK59 pKa = 10.85HH60 pKa = 6.69VPITDD65 pKa = 3.43KK66 pKa = 11.57GFMALSKK73 pKa = 10.6RR74 pKa = 11.84DD75 pKa = 3.27VQLFFKK81 pKa = 10.56RR82 pKa = 11.84LRR84 pKa = 11.84KK85 pKa = 9.62AHH87 pKa = 5.82TKK89 pKa = 9.73EE90 pKa = 3.83QLSDD94 pKa = 3.71NPIKK98 pKa = 10.92YY99 pKa = 9.7YY100 pKa = 10.7VAGEE104 pKa = 3.87YY105 pKa = 10.5GGRR108 pKa = 11.84TNRR111 pKa = 11.84PHH113 pKa = 5.53YY114 pKa = 10.45HH115 pKa = 6.74IILFNAVLEE124 pKa = 4.85LIQPAWNLGSVHH136 pKa = 6.24YY137 pKa = 11.09GEE139 pKa = 4.93VNEE142 pKa = 4.45ASVGYY147 pKa = 6.83TLKK150 pKa = 10.98YY151 pKa = 7.06MCKK154 pKa = 8.42PARR157 pKa = 11.84IPLHH161 pKa = 6.83ANDD164 pKa = 4.19DD165 pKa = 4.01RR166 pKa = 11.84QPEE169 pKa = 4.06FSLMSKK175 pKa = 10.51GLAATTT181 pKa = 3.82

Molecular weight: 20.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1185

80

507

237.0

26.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.27 ± 1.129

0.928 ± 0.501

4.979 ± 0.444

6.245 ± 0.989

3.966 ± 1.014

6.329 ± 0.526

2.11 ± 0.713

5.738 ± 0.42

6.16 ± 0.947

7.342 ± 0.61

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.038 ± 0.12

5.485 ± 0.405

6.414 ± 0.821

5.823 ± 0.758

5.485 ± 0.346

6.16 ± 0.725

5.401 ± 0.568

4.726 ± 0.492

1.266 ± 0.175

4.135 ± 0.55
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski