Balaenoptera physalus (Fin whale) (Balaena physalus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Artiodactyla

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A1Q679|A0A6A1Q679_BALPH DUF4587 domain-containing protein (Fragment) OS=Balaenoptera physalus OX=9770 GN=E2I00_009667 PE=4 SV=1
MM1 pKa = 7.63NNLQYY6 pKa = 11.2YY7 pKa = 10.0DD8 pKa = 3.87ISAKK12 pKa = 10.55SNYY15 pKa = 9.3NFEE18 pKa = 5.67KK19 pKa = 10.42PFLWLARR26 pKa = 11.84NLIGDD31 pKa = 4.1PNLEE35 pKa = 4.15FVTMSALTPPEE46 pKa = 4.2AVMDD50 pKa = 4.46PATAAQYY57 pKa = 9.02EE58 pKa = 4.65HH59 pKa = 7.19YY60 pKa = 10.62LEE62 pKa = 4.86VVQTTALPDD71 pKa = 3.71EE72 pKa = 5.7DD73 pKa = 5.98DD74 pKa = 4.52DD75 pKa = 4.83LL76 pKa = 6.68

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A1Q7B1|A0A6A1Q7B1_BALPH Sema domain-containing protein OS=Balaenoptera physalus OX=9770 GN=E2I00_011180 PE=4 SV=1
AA1 pKa = 6.91TTFLNVATPPGISNWPKK18 pKa = 10.32LVLSAPSLGSEE29 pKa = 3.78PRR31 pKa = 11.84AEE33 pKa = 4.08KK34 pKa = 10.47RR35 pKa = 11.84PPLRR39 pKa = 11.84PEE41 pKa = 3.33EE42 pKa = 3.84WDD44 pKa = 3.14FRR46 pKa = 11.84TRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84GRR52 pKa = 11.84RR53 pKa = 11.84PMRR56 pKa = 11.84QSQDD60 pKa = 3.34LKK62 pKa = 10.8GGPPSSPAEE71 pKa = 3.87AGFSRR76 pKa = 11.84RR77 pKa = 11.84GRR79 pKa = 11.84SFGLKK84 pKa = 9.64GVAGPKK90 pKa = 9.49LKK92 pKa = 10.44KK93 pKa = 8.77HH94 pKa = 5.88CCYY97 pKa = 10.72VSLDD101 pKa = 3.7FEE103 pKa = 5.85GDD105 pKa = 3.1LRR107 pKa = 11.84NPARR111 pKa = 11.84HH112 pKa = 6.13HH113 pKa = 6.28PASFYY118 pKa = 10.9LGNGCSVCLSSEE130 pKa = 4.07RR131 pKa = 11.84FRR133 pKa = 11.84CPEE136 pKa = 4.66PIFQPGLLGQAEE148 pKa = 4.44PGLPTLAFRR157 pKa = 11.84ALQRR161 pKa = 11.84MPATLRR167 pKa = 11.84TRR169 pKa = 11.84LANTVVVAGGSTLFPGFTEE188 pKa = 3.95RR189 pKa = 11.84LEE191 pKa = 4.28MEE193 pKa = 5.32LEE195 pKa = 4.28AQCRR199 pKa = 11.84RR200 pKa = 11.84HH201 pKa = 6.49GYY203 pKa = 9.76GALQPRR209 pKa = 11.84LVAKK213 pKa = 9.77PGRR216 pKa = 11.84GTAVWTGGSMVASLRR231 pKa = 11.84SFQCHH236 pKa = 3.71WMTRR240 pKa = 11.84AMYY243 pKa = 9.87QEE245 pKa = 4.09YY246 pKa = 10.42GSRR249 pKa = 11.84LVHH252 pKa = 6.12EE253 pKa = 4.65VFKK256 pKa = 11.31

Molecular weight: 28.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19643

0

19643

8174059

9

7039

416.1

46.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.892 ± 0.016

2.211 ± 0.014

4.777 ± 0.012

7.02 ± 0.025

3.682 ± 0.014

6.56 ± 0.025

2.569 ± 0.008

4.378 ± 0.016

5.779 ± 0.023

10.04 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.007

3.573 ± 0.011

6.128 ± 0.026

4.705 ± 0.016

5.65 ± 0.016

8.22 ± 0.022

5.403 ± 0.03

6.136 ± 0.014

1.267 ± 0.006

2.592 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski