Physostegia chlorotic mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Alphanucleorhabdovirus; Physostegia chlorotic mottle alphanucleorhabdovirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8FVH3|A0A1D8FVH3_9RHAB Putative movement protein OS=Physostegia chlorotic mottle virus OX=1905830 GN=Y PE=4 SV=1
MM1 pKa = 7.18SASRR5 pKa = 11.84NTTTAPDD12 pKa = 3.52KK13 pKa = 10.79PGNQGDD19 pKa = 3.91EE20 pKa = 4.45IFIEE24 pKa = 4.33MCRR27 pKa = 11.84MVDD30 pKa = 3.21NLLSEE35 pKa = 4.43KK36 pKa = 10.39EE37 pKa = 4.13KK38 pKa = 10.52PDD40 pKa = 5.4PINQSYY46 pKa = 9.3PRR48 pKa = 11.84QSQEE52 pKa = 5.4DD53 pKa = 4.07EE54 pKa = 4.24NQPSDD59 pKa = 3.87TEE61 pKa = 4.44STEE64 pKa = 3.24SWTYY68 pKa = 11.24YY69 pKa = 10.74DD70 pKa = 4.12YY71 pKa = 11.21TDD73 pKa = 2.87HH74 pKa = 7.56RR75 pKa = 11.84YY76 pKa = 9.38YY77 pKa = 10.26PSYY80 pKa = 10.64EE81 pKa = 4.57DD82 pKa = 4.16NDD84 pKa = 3.78SDD86 pKa = 5.38IYY88 pKa = 11.69DD89 pKa = 4.05LMYY92 pKa = 10.64EE93 pKa = 4.44CNHH96 pKa = 6.31GEE98 pKa = 3.85WGNN101 pKa = 3.42

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8FVQ4|A0A1D8FVQ4_9RHAB Large structural protein OS=Physostegia chlorotic mottle virus OX=1905830 GN=L PE=4 SV=1
MM1 pKa = 7.46INVIGKK7 pKa = 8.02TNASVQGIYY16 pKa = 10.28EE17 pKa = 3.86VDD19 pKa = 3.56RR20 pKa = 11.84EE21 pKa = 4.41KK22 pKa = 11.31VSLLSPVTGQFVSLNYY38 pKa = 9.65IIKK41 pKa = 9.61INMYY45 pKa = 8.84EE46 pKa = 4.48KK47 pKa = 10.56EE48 pKa = 4.09MQKK51 pKa = 10.34SFEE54 pKa = 4.17EE55 pKa = 5.12GEE57 pKa = 4.17LHH59 pKa = 5.88YY60 pKa = 10.83HH61 pKa = 7.5DD62 pKa = 4.83IFDD65 pKa = 4.08MVEE68 pKa = 3.92SALNLPTTRR77 pKa = 11.84PDD79 pKa = 3.62SIKK82 pKa = 9.96RR83 pKa = 11.84TPTNLPTRR91 pKa = 11.84VAKK94 pKa = 9.57TIIQICRR101 pKa = 11.84IHH103 pKa = 7.41AIHH106 pKa = 6.74TNAKK110 pKa = 8.02MVFMTTSTDD119 pKa = 3.19HH120 pKa = 8.05SEE122 pKa = 4.11DD123 pKa = 3.74TPTLVIKK130 pKa = 10.57EE131 pKa = 4.35GEE133 pKa = 4.47TLVSPHH139 pKa = 6.58YY140 pKa = 9.59DD141 pKa = 3.03SHH143 pKa = 7.48VVEE146 pKa = 5.1VVNQPGEE153 pKa = 4.4SPLSGSYY160 pKa = 10.53HH161 pKa = 5.66LTLEE165 pKa = 4.41KK166 pKa = 10.41PYY168 pKa = 10.27ISQDD172 pKa = 3.01NKK174 pKa = 10.8IIGHH178 pKa = 6.99LSFAAYY184 pKa = 9.66VRR186 pKa = 11.84APPRR190 pKa = 11.84GNAVSQARR198 pKa = 11.84LNIRR202 pKa = 11.84VLASKK207 pKa = 8.1YY208 pKa = 8.7QRR210 pKa = 11.84SAAHH214 pKa = 6.57KK215 pKa = 10.27VNTLFRR221 pKa = 11.84KK222 pKa = 8.92SMKK225 pKa = 9.84RR226 pKa = 11.84KK227 pKa = 10.17SEE229 pKa = 4.05TSPTKK234 pKa = 10.63APSGSIMDD242 pKa = 4.37AVAKK246 pKa = 10.14ILKK249 pKa = 9.97QSS251 pKa = 3.17

Molecular weight: 28.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3979

101

1946

568.4

64.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.976 ± 1.137

1.608 ± 0.398

6.132 ± 0.464

5.454 ± 0.535

3.418 ± 0.303

5.504 ± 0.352

2.312 ± 0.515

7.439 ± 0.384

5.604 ± 0.553

8.444 ± 0.994

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.971 ± 0.359

4.85 ± 0.649

5.077 ± 0.616

3.192 ± 0.279

4.775 ± 0.68

9.324 ± 0.626

6.032 ± 0.56

6.685 ± 0.505

1.206 ± 0.311

3.996 ± 0.607
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski