Amino acid dipepetide frequency for Gordonia phage Gmala1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.679AlaAla: 11.679 ± 2.566
0.661AlaCys: 0.661 ± 0.229
5.509AlaAsp: 5.509 ± 0.509
5.906AlaGlu: 5.906 ± 0.656
2.909AlaPhe: 2.909 ± 0.314
8.021AlaGly: 8.021 ± 1.294
1.146AlaHis: 1.146 ± 0.205
4.76AlaIle: 4.76 ± 0.683
4.275AlaLys: 4.275 ± 0.591
7.007AlaLeu: 7.007 ± 0.72
1.851AlaMet: 1.851 ± 0.403
3.79AlaAsn: 3.79 ± 0.385
4.319AlaPro: 4.319 ± 0.679
4.143AlaGln: 4.143 ± 0.907
4.363AlaArg: 4.363 ± 0.399
5.641AlaSer: 5.641 ± 0.636
4.936AlaThr: 4.936 ± 0.577
6.126AlaVal: 6.126 ± 0.475
1.058AlaTrp: 1.058 ± 0.231
1.983AlaTyr: 1.983 ± 0.241
0.0AlaXaa: 0.0 ± 0.0
Cys
0.573CysAla: 0.573 ± 0.179
0.176CysCys: 0.176 ± 0.102
0.705CysAsp: 0.705 ± 0.235
0.837CysGlu: 0.837 ± 0.265
0.353CysPhe: 0.353 ± 0.144
0.749CysGly: 0.749 ± 0.198
0.088CysHis: 0.088 ± 0.061
0.529CysIle: 0.529 ± 0.178
0.617CysLys: 0.617 ± 0.201
0.485CysLeu: 0.485 ± 0.185
0.176CysMet: 0.176 ± 0.084
0.485CysAsn: 0.485 ± 0.188
0.661CysPro: 0.661 ± 0.216
0.397CysGln: 0.397 ± 0.162
0.793CysArg: 0.793 ± 0.275
0.705CysSer: 0.705 ± 0.222
0.573CysThr: 0.573 ± 0.179
0.529CysVal: 0.529 ± 0.178
0.132CysTrp: 0.132 ± 0.078
0.176CysTyr: 0.176 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
4.363AspAla: 4.363 ± 0.406
0.397AspCys: 0.397 ± 0.145
4.407AspAsp: 4.407 ± 0.745
4.495AspGlu: 4.495 ± 0.487
2.556AspPhe: 2.556 ± 0.339
4.495AspGly: 4.495 ± 0.627
1.895AspHis: 1.895 ± 0.421
4.011AspIle: 4.011 ± 0.416
3.834AspLys: 3.834 ± 0.408
5.068AspLeu: 5.068 ± 0.448
1.454AspMet: 1.454 ± 0.284
2.556AspAsn: 2.556 ± 0.553
4.716AspPro: 4.716 ± 0.668
2.115AspGln: 2.115 ± 0.496
3.085AspArg: 3.085 ± 0.457
5.112AspSer: 5.112 ± 1.636
3.57AspThr: 3.57 ± 0.347
4.099AspVal: 4.099 ± 0.428
1.498AspTrp: 1.498 ± 0.305
2.512AspTyr: 2.512 ± 0.438
0.0AspXaa: 0.0 ± 0.0
Glu
4.76GluAla: 4.76 ± 0.6
0.661GluCys: 0.661 ± 0.188
3.834GluAsp: 3.834 ± 0.487
3.967GluGlu: 3.967 ± 0.462
2.732GluPhe: 2.732 ± 0.496
2.732GluGly: 2.732 ± 0.379
0.881GluHis: 0.881 ± 0.232
4.936GluIle: 4.936 ± 0.527
4.275GluLys: 4.275 ± 0.461
5.509GluLeu: 5.509 ± 0.58
2.16GluMet: 2.16 ± 0.254
4.495GluAsn: 4.495 ± 0.433
2.027GluPro: 2.027 ± 0.365
2.424GluGln: 2.424 ± 0.432
3.041GluArg: 3.041 ± 0.389
3.967GluSer: 3.967 ± 0.467
3.305GluThr: 3.305 ± 0.39
3.658GluVal: 3.658 ± 0.496
1.41GluTrp: 1.41 ± 0.25
1.719GluTyr: 1.719 ± 0.308
0.0GluXaa: 0.0 ± 0.0
Phe
2.909PheAla: 2.909 ± 0.365
0.397PheCys: 0.397 ± 0.124
3.129PheAsp: 3.129 ± 0.439
2.204PheGlu: 2.204 ± 0.349
1.939PhePhe: 1.939 ± 0.277
3.085PheGly: 3.085 ± 0.364
0.837PheHis: 0.837 ± 0.186
1.807PheIle: 1.807 ± 0.278
2.292PheLys: 2.292 ± 0.415
2.556PheLeu: 2.556 ± 0.349
1.498PheMet: 1.498 ± 0.275
2.336PheAsn: 2.336 ± 0.306
1.587PhePro: 1.587 ± 0.283
1.146PheGln: 1.146 ± 0.248
1.807PheArg: 1.807 ± 0.277
3.658PheSer: 3.658 ± 0.535
2.38PheThr: 2.38 ± 0.346
2.512PheVal: 2.512 ± 0.351
0.617PheTrp: 0.617 ± 0.161
0.837PheTyr: 0.837 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
5.906GlyAla: 5.906 ± 0.955
0.573GlyCys: 0.573 ± 0.187
4.892GlyAsp: 4.892 ± 0.574
4.143GlyGlu: 4.143 ± 0.385
2.865GlyPhe: 2.865 ± 0.322
6.082GlyGly: 6.082 ± 1.181
1.41GlyHis: 1.41 ± 0.29
5.289GlyIle: 5.289 ± 0.481
5.377GlyLys: 5.377 ± 0.517
5.333GlyLeu: 5.333 ± 0.624
2.6GlyMet: 2.6 ± 0.29
3.349GlyAsn: 3.349 ± 0.449
3.041GlyPro: 3.041 ± 0.311
2.512GlyGln: 2.512 ± 0.341
3.482GlyArg: 3.482 ± 0.4
5.245GlySer: 5.245 ± 0.524
5.906GlyThr: 5.906 ± 0.718
4.672GlyVal: 4.672 ± 0.507
1.498GlyTrp: 1.498 ± 0.259
2.777GlyTyr: 2.777 ± 0.321
0.0GlyXaa: 0.0 ± 0.0
His
1.41HisAla: 1.41 ± 0.304
0.088HisCys: 0.088 ± 0.067
1.498HisAsp: 1.498 ± 0.357
1.234HisGlu: 1.234 ± 0.254
0.573HisPhe: 0.573 ± 0.192
1.234HisGly: 1.234 ± 0.245
0.176HisHis: 0.176 ± 0.092
0.837HisIle: 0.837 ± 0.249
1.234HisLys: 1.234 ± 0.262
1.014HisLeu: 1.014 ± 0.229
0.617HisMet: 0.617 ± 0.158
0.793HisAsn: 0.793 ± 0.187
1.322HisPro: 1.322 ± 0.323
0.529HisGln: 0.529 ± 0.121
0.837HisArg: 0.837 ± 0.233
0.926HisSer: 0.926 ± 0.231
0.926HisThr: 0.926 ± 0.157
1.146HisVal: 1.146 ± 0.244
0.353HisTrp: 0.353 ± 0.129
0.441HisTyr: 0.441 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
5.024IleAla: 5.024 ± 0.41
0.705IleCys: 0.705 ± 0.256
3.57IleAsp: 3.57 ± 0.311
3.57IleGlu: 3.57 ± 0.32
2.16IlePhe: 2.16 ± 0.304
4.143IleGly: 4.143 ± 0.761
1.366IleHis: 1.366 ± 0.308
3.261IleIle: 3.261 ± 0.374
3.217IleLys: 3.217 ± 0.406
4.319IleLeu: 4.319 ± 0.447
1.41IleMet: 1.41 ± 0.3
2.865IleAsn: 2.865 ± 0.329
3.482IlePro: 3.482 ± 0.538
2.16IleGln: 2.16 ± 0.295
3.085IleArg: 3.085 ± 0.351
4.143IleSer: 4.143 ± 0.578
3.922IleThr: 3.922 ± 0.503
3.261IleVal: 3.261 ± 0.378
0.881IleTrp: 0.881 ± 0.246
1.543IleTyr: 1.543 ± 0.378
0.0IleXaa: 0.0 ± 0.0
Lys
5.112LysAla: 5.112 ± 0.969
0.529LysCys: 0.529 ± 0.213
3.349LysAsp: 3.349 ± 0.606
2.468LysGlu: 2.468 ± 0.366
3.349LysPhe: 3.349 ± 0.365
3.614LysGly: 3.614 ± 0.523
1.498LysHis: 1.498 ± 0.312
3.305LysIle: 3.305 ± 0.404
4.275LysLys: 4.275 ± 0.54
4.055LysLeu: 4.055 ± 0.462
1.763LysMet: 1.763 ± 0.275
3.57LysAsn: 3.57 ± 0.295
2.248LysPro: 2.248 ± 0.399
2.424LysGln: 2.424 ± 0.311
3.658LysArg: 3.658 ± 0.449
3.614LysSer: 3.614 ± 0.338
3.305LysThr: 3.305 ± 0.43
3.217LysVal: 3.217 ± 0.389
1.19LysTrp: 1.19 ± 0.202
1.366LysTyr: 1.366 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
6.479LeuAla: 6.479 ± 0.596
0.97LeuCys: 0.97 ± 0.243
5.333LeuAsp: 5.333 ± 0.464
5.024LeuGlu: 5.024 ± 0.604
2.777LeuPhe: 2.777 ± 0.341
5.95LeuGly: 5.95 ± 0.597
1.014LeuHis: 1.014 ± 0.251
3.79LeuIle: 3.79 ± 0.459
4.011LeuLys: 4.011 ± 0.476
4.76LeuLeu: 4.76 ± 0.442
1.807LeuMet: 1.807 ± 0.285
4.099LeuAsn: 4.099 ± 0.522
4.407LeuPro: 4.407 ± 0.407
2.248LeuGln: 2.248 ± 0.36
3.173LeuArg: 3.173 ± 0.415
5.068LeuSer: 5.068 ± 0.412
4.804LeuThr: 4.804 ± 0.471
5.024LeuVal: 5.024 ± 0.482
0.749LeuTrp: 0.749 ± 0.153
1.763LeuTyr: 1.763 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
2.997MetAla: 2.997 ± 0.356
0.353MetCys: 0.353 ± 0.137
1.719MetAsp: 1.719 ± 0.269
1.587MetGlu: 1.587 ± 0.298
0.705MetPhe: 0.705 ± 0.154
2.027MetGly: 2.027 ± 0.299
0.397MetHis: 0.397 ± 0.151
1.41MetIle: 1.41 ± 0.25
1.498MetLys: 1.498 ± 0.246
2.468MetLeu: 2.468 ± 0.338
0.793MetMet: 0.793 ± 0.186
1.454MetAsn: 1.454 ± 0.259
1.322MetPro: 1.322 ± 0.271
0.881MetGln: 0.881 ± 0.213
1.587MetArg: 1.587 ± 0.246
2.38MetSer: 2.38 ± 0.265
1.939MetThr: 1.939 ± 0.254
1.763MetVal: 1.763 ± 0.255
0.309MetTrp: 0.309 ± 0.129
0.353MetTyr: 0.353 ± 0.161
0.0MetXaa: 0.0 ± 0.0
Asn
4.363AsnAla: 4.363 ± 0.821
0.264AsnCys: 0.264 ± 0.161
3.041AsnAsp: 3.041 ± 0.445
2.688AsnGlu: 2.688 ± 0.4
2.204AsnPhe: 2.204 ± 0.401
4.407AsnGly: 4.407 ± 0.375
0.881AsnHis: 0.881 ± 0.245
2.732AsnIle: 2.732 ± 0.396
2.6AsnLys: 2.6 ± 0.357
3.57AsnLeu: 3.57 ± 0.434
1.543AsnMet: 1.543 ± 0.28
2.556AsnAsn: 2.556 ± 0.421
2.688AsnPro: 2.688 ± 0.319
2.071AsnGln: 2.071 ± 0.313
2.468AsnArg: 2.468 ± 0.291
3.878AsnSer: 3.878 ± 0.505
3.129AsnThr: 3.129 ± 0.309
2.997AsnVal: 2.997 ± 0.402
0.353AsnTrp: 0.353 ± 0.118
1.498AsnTyr: 1.498 ± 0.267
0.0AsnXaa: 0.0 ± 0.0
Pro
4.628ProAla: 4.628 ± 0.714
0.529ProCys: 0.529 ± 0.149
3.217ProAsp: 3.217 ± 0.433
3.526ProGlu: 3.526 ± 0.397
1.719ProPhe: 1.719 ± 0.363
4.363ProGly: 4.363 ± 0.429
0.926ProHis: 0.926 ± 0.241
2.556ProIle: 2.556 ± 0.389
2.512ProLys: 2.512 ± 0.373
2.865ProLeu: 2.865 ± 0.398
1.146ProMet: 1.146 ± 0.206
2.336ProAsn: 2.336 ± 0.323
2.204ProPro: 2.204 ± 0.528
1.763ProGln: 1.763 ± 0.239
1.983ProArg: 1.983 ± 0.385
2.777ProSer: 2.777 ± 0.447
2.468ProThr: 2.468 ± 0.357
4.319ProVal: 4.319 ± 0.716
0.661ProTrp: 0.661 ± 0.205
1.19ProTyr: 1.19 ± 0.221
0.0ProXaa: 0.0 ± 0.0
Gln
3.922GlnAla: 3.922 ± 0.615
0.353GlnCys: 0.353 ± 0.153
2.6GlnAsp: 2.6 ± 0.782
2.6GlnGlu: 2.6 ± 0.341
2.027GlnPhe: 2.027 ± 0.276
2.248GlnGly: 2.248 ± 0.297
0.309GlnHis: 0.309 ± 0.143
2.204GlnIle: 2.204 ± 0.366
2.248GlnLys: 2.248 ± 0.311
2.777GlnLeu: 2.777 ± 0.421
1.366GlnMet: 1.366 ± 0.299
1.675GlnAsn: 1.675 ± 0.239
1.851GlnPro: 1.851 ± 0.413
1.675GlnGln: 1.675 ± 0.367
1.851GlnArg: 1.851 ± 0.255
1.543GlnSer: 1.543 ± 0.277
1.763GlnThr: 1.763 ± 0.34
2.6GlnVal: 2.6 ± 0.329
0.529GlnTrp: 0.529 ± 0.138
1.543GlnTyr: 1.543 ± 0.345
0.0GlnXaa: 0.0 ± 0.0
Arg
4.98ArgAla: 4.98 ± 0.584
0.573ArgCys: 0.573 ± 0.181
2.997ArgAsp: 2.997 ± 0.377
3.217ArgGlu: 3.217 ± 0.497
1.983ArgPhe: 1.983 ± 0.311
3.261ArgGly: 3.261 ± 0.375
0.793ArgHis: 0.793 ± 0.194
3.57ArgIle: 3.57 ± 0.401
3.658ArgLys: 3.658 ± 0.421
3.349ArgLeu: 3.349 ± 0.412
1.587ArgMet: 1.587 ± 0.29
2.6ArgAsn: 2.6 ± 0.425
2.336ArgPro: 2.336 ± 0.336
1.587ArgGln: 1.587 ± 0.234
2.732ArgArg: 2.732 ± 0.293
2.953ArgSer: 2.953 ± 0.301
2.38ArgThr: 2.38 ± 0.477
3.526ArgVal: 3.526 ± 0.418
0.661ArgTrp: 0.661 ± 0.179
1.939ArgTyr: 1.939 ± 0.329
0.0ArgXaa: 0.0 ± 0.0
Ser
6.435SerAla: 6.435 ± 0.639
0.749SerCys: 0.749 ± 0.208
4.672SerAsp: 4.672 ± 0.858
3.79SerGlu: 3.79 ± 0.731
2.424SerPhe: 2.424 ± 0.297
6.787SerGly: 6.787 ± 0.726
0.926SerHis: 0.926 ± 0.212
3.394SerIle: 3.394 ± 0.394
3.834SerLys: 3.834 ± 0.495
5.509SerLeu: 5.509 ± 0.482
2.027SerMet: 2.027 ± 0.311
2.512SerAsn: 2.512 ± 0.368
2.512SerPro: 2.512 ± 0.479
3.041SerGln: 3.041 ± 0.39
3.394SerArg: 3.394 ± 0.463
3.878SerSer: 3.878 ± 0.502
3.658SerThr: 3.658 ± 0.463
4.628SerVal: 4.628 ± 0.426
0.837SerTrp: 0.837 ± 0.189
2.248SerTyr: 2.248 ± 0.253
0.0SerXaa: 0.0 ± 0.0
Thr
5.906ThrAla: 5.906 ± 0.828
0.485ThrCys: 0.485 ± 0.163
3.922ThrAsp: 3.922 ± 0.645
4.451ThrGlu: 4.451 ± 0.456
1.763ThrPhe: 1.763 ± 0.24
5.509ThrGly: 5.509 ± 0.531
0.881ThrHis: 0.881 ± 0.243
2.424ThrIle: 2.424 ± 0.389
2.909ThrLys: 2.909 ± 0.355
3.702ThrLeu: 3.702 ± 0.369
1.322ThrMet: 1.322 ± 0.263
2.732ThrAsn: 2.732 ± 0.376
2.953ThrPro: 2.953 ± 0.35
2.644ThrGln: 2.644 ± 0.276
2.865ThrArg: 2.865 ± 0.31
3.79ThrSer: 3.79 ± 0.353
2.6ThrThr: 2.6 ± 0.422
4.584ThrVal: 4.584 ± 0.485
0.793ThrTrp: 0.793 ± 0.2
1.498ThrTyr: 1.498 ± 0.206
0.0ThrXaa: 0.0 ± 0.0
Val
5.333ValAla: 5.333 ± 0.724
0.617ValCys: 0.617 ± 0.168
4.672ValAsp: 4.672 ± 0.528
4.319ValGlu: 4.319 ± 0.453
2.688ValPhe: 2.688 ± 0.376
4.231ValGly: 4.231 ± 0.661
1.234ValHis: 1.234 ± 0.337
4.055ValIle: 4.055 ± 0.382
2.644ValLys: 2.644 ± 0.383
5.289ValLeu: 5.289 ± 0.625
1.719ValMet: 1.719 ± 0.308
3.79ValAsn: 3.79 ± 0.354
2.777ValPro: 2.777 ± 0.285
2.292ValGln: 2.292 ± 0.367
3.129ValArg: 3.129 ± 0.417
5.068ValSer: 5.068 ± 0.473
4.099ValThr: 4.099 ± 0.436
4.319ValVal: 4.319 ± 0.442
0.837ValTrp: 0.837 ± 0.223
2.38ValTyr: 2.38 ± 0.309
0.0ValXaa: 0.0 ± 0.0
Trp
1.278TrpAla: 1.278 ± 0.221
0.176TrpCys: 0.176 ± 0.094
1.234TrpAsp: 1.234 ± 0.19
0.837TrpGlu: 0.837 ± 0.169
0.749TrpPhe: 0.749 ± 0.222
1.058TrpGly: 1.058 ± 0.225
0.22TrpHis: 0.22 ± 0.099
1.146TrpIle: 1.146 ± 0.301
0.705TrpLys: 0.705 ± 0.19
1.146TrpLeu: 1.146 ± 0.214
0.353TrpMet: 0.353 ± 0.13
0.97TrpAsn: 0.97 ± 0.17
0.309TrpPro: 0.309 ± 0.135
0.617TrpGln: 0.617 ± 0.17
1.058TrpArg: 1.058 ± 0.211
1.146TrpSer: 1.146 ± 0.223
0.529TrpThr: 0.529 ± 0.162
0.617TrpVal: 0.617 ± 0.144
0.0TrpTrp: 0.0 ± 0.0
0.661TrpTyr: 0.661 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.027TyrAla: 2.027 ± 0.3
0.485TyrCys: 0.485 ± 0.18
1.939TyrAsp: 1.939 ± 0.277
1.631TyrGlu: 1.631 ± 0.251
1.058TyrPhe: 1.058 ± 0.184
2.953TyrGly: 2.953 ± 0.359
0.309TyrHis: 0.309 ± 0.142
2.071TyrIle: 2.071 ± 0.316
1.895TyrLys: 1.895 ± 0.263
2.468TyrLeu: 2.468 ± 0.346
0.705TyrMet: 0.705 ± 0.189
1.014TyrAsn: 1.014 ± 0.254
0.926TyrPro: 0.926 ± 0.257
1.014TyrGln: 1.014 ± 0.242
2.292TyrArg: 2.292 ± 0.371
1.763TyrSer: 1.763 ± 0.374
1.543TyrThr: 1.543 ± 0.378
1.895TyrVal: 1.895 ± 0.345
0.441TyrTrp: 0.441 ± 0.123
0.881TyrTyr: 0.881 ± 0.232
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (22691 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski