Amino acid dipepetide frequency for Bacillus sp. MUM 116

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.997AlaAla: 5.997 ± 0.088	0.585AlaCys: 0.585 ± 0.019	3.158AlaAsp: 3.158 ± 0.051	4.463AlaGlu: 4.463 ± 0.06	3.292AlaPhe: 3.292 ± 0.054	5.421AlaGly: 5.421 ± 0.077	1.231AlaHis: 1.231 ± 0.034	6.088AlaIle: 6.088 ± 0.074	4.907AlaLys: 4.907 ± 0.063	6.88AlaLeu: 6.88 ± 0.083	1.95AlaMet: 1.95 ± 0.041	2.864AlaAsn: 2.864 ± 0.043	2.129AlaPro: 2.129 ± 0.041	2.073AlaGln: 2.073 ± 0.041	2.438AlaArg: 2.438 ± 0.045	3.968AlaSer: 3.968 ± 0.052	3.217AlaThr: 3.217 ± 0.05	5.282AlaVal: 5.282 ± 0.068	0.609AlaTrp: 0.609 ± 0.023	2.245AlaTyr: 2.245 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	0.438CysAla: 0.438 ± 0.018	0.112CysCys: 0.112 ± 0.01	0.405CysAsp: 0.405 ± 0.015	0.472CysGlu: 0.472 ± 0.018	0.382CysPhe: 0.382 ± 0.017	0.712CysGly: 0.712 ± 0.023	0.208CysHis: 0.208 ± 0.012	0.609CysIle: 0.609 ± 0.02	0.398CysLys: 0.398 ± 0.018	0.771CysLeu: 0.771 ± 0.021	0.177CysMet: 0.177 ± 0.01	0.313CysAsn: 0.313 ± 0.015	0.372CysPro: 0.372 ± 0.016	0.225CysGln: 0.225 ± 0.013	0.27CysArg: 0.27 ± 0.014	0.535CysSer: 0.535 ± 0.018	0.389CysThr: 0.389 ± 0.017	0.423CysVal: 0.423 ± 0.018	0.082CysTrp: 0.082 ± 0.006	0.284CysTyr: 0.284 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.015AspAla: 3.015 ± 0.055	0.382AspCys: 0.382 ± 0.017	2.052AspAsp: 2.052 ± 0.041	3.754AspGlu: 3.754 ± 0.057	2.496AspPhe: 2.496 ± 0.042	3.158AspGly: 3.158 ± 0.054	1.163AspHis: 1.163 ± 0.028	3.723AspIle: 3.723 ± 0.051	2.959AspLys: 2.959 ± 0.051	4.971AspLeu: 4.971 ± 0.056	1.197AspMet: 1.197 ± 0.033	1.642AspAsn: 1.642 ± 0.038	1.93AspPro: 1.93 ± 0.043	1.955AspGln: 1.955 ± 0.036	2.066AspArg: 2.066 ± 0.036	2.552AspSer: 2.552 ± 0.044	2.162AspThr: 2.162 ± 0.04	3.433AspVal: 3.433 ± 0.049	0.618AspTrp: 0.618 ± 0.023	1.967AspTyr: 1.967 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	4.78GluAla: 4.78 ± 0.063	0.404GluCys: 0.404 ± 0.016	3.173GluAsp: 3.173 ± 0.058	6.112GluGlu: 6.112 ± 0.091	2.653GluPhe: 2.653 ± 0.044	4.022GluGly: 4.022 ± 0.059	1.418GluHis: 1.418 ± 0.032	5.799GluIle: 5.799 ± 0.067	6.852GluLys: 6.852 ± 0.076	6.845GluLeu: 6.845 ± 0.08	2.236GluMet: 2.236 ± 0.04	3.75GluAsn: 3.75 ± 0.052	1.797GluPro: 1.797 ± 0.038	2.857GluGln: 2.857 ± 0.048	3.105GluArg: 3.105 ± 0.056	3.287GluSer: 3.287 ± 0.054	3.595GluThr: 3.595 ± 0.049	4.595GluVal: 4.595 ± 0.059	0.752GluTrp: 0.752 ± 0.021	2.203GluTyr: 2.203 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	3.153PheAla: 3.153 ± 0.057	0.41PheCys: 0.41 ± 0.018	2.33PheAsp: 2.33 ± 0.038	2.856PheGlu: 2.856 ± 0.042	2.594PhePhe: 2.594 ± 0.05	3.609PheGly: 3.609 ± 0.057	1.135PheHis: 1.135 ± 0.026	4.203PheIle: 4.203 ± 0.062	2.631PheLys: 2.631 ± 0.04	5.089PheLeu: 5.089 ± 0.077	1.267PheMet: 1.267 ± 0.029	1.984PheAsn: 1.984 ± 0.04	1.916PhePro: 1.916 ± 0.038	1.622PheGln: 1.622 ± 0.032	1.637PheArg: 1.637 ± 0.032	3.495PheSer: 3.495 ± 0.043	2.648PheThr: 2.648 ± 0.042	3.288PheVal: 3.288 ± 0.056	0.535PheTrp: 0.535 ± 0.02	1.867PheTyr: 1.867 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.965GlyAla: 4.965 ± 0.073	0.651GlyCys: 0.651 ± 0.023	2.966GlyAsp: 2.966 ± 0.046	4.271GlyGlu: 4.271 ± 0.059	3.72GlyPhe: 3.72 ± 0.06	4.979GlyGly: 4.979 ± 0.076	1.414GlyHis: 1.414 ± 0.031	6.398GlyIle: 6.398 ± 0.08	5.536GlyLys: 5.536 ± 0.054	6.841GlyLeu: 6.841 ± 0.078	2.165GlyMet: 2.165 ± 0.042	3.014GlyAsn: 3.014 ± 0.051	1.865GlyPro: 1.865 ± 0.033	2.121GlyGln: 2.121 ± 0.04	2.569GlyArg: 2.569 ± 0.046	4.025GlySer: 4.025 ± 0.061	4.125GlyThr: 4.125 ± 0.063	5.095GlyVal: 5.095 ± 0.064	0.89GlyTrp: 0.89 ± 0.023	2.838GlyTyr: 2.838 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.212HisAla: 1.212 ± 0.029	0.205HisCys: 0.205 ± 0.011	1.02HisAsp: 1.02 ± 0.03	1.294HisGlu: 1.294 ± 0.032	1.124HisPhe: 1.124 ± 0.022	1.496HisGly: 1.496 ± 0.036	0.658HisHis: 0.658 ± 0.024	1.507HisIle: 1.507 ± 0.033	1.07HisLys: 1.07 ± 0.027	2.185HisLeu: 2.185 ± 0.037	0.499HisMet: 0.499 ± 0.019	0.773HisAsn: 0.773 ± 0.023	1.18HisPro: 1.18 ± 0.028	0.86HisGln: 0.86 ± 0.023	0.871HisArg: 0.871 ± 0.023	1.286HisSer: 1.286 ± 0.029	0.986HisThr: 0.986 ± 0.022	1.324HisVal: 1.324 ± 0.033	0.222HisTrp: 0.222 ± 0.011	0.919HisTyr: 0.919 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.14IleAla: 6.14 ± 0.07	0.726IleCys: 0.726 ± 0.02	4.137IleAsp: 4.137 ± 0.065	5.49IleGlu: 5.49 ± 0.069	3.725IlePhe: 3.725 ± 0.057	6.337IleGly: 6.337 ± 0.075	1.854IleHis: 1.854 ± 0.037	6.348IleIle: 6.348 ± 0.074	5.082IleLys: 5.082 ± 0.061	7.949IleLeu: 7.949 ± 0.079	1.942IleMet: 1.942 ± 0.036	3.609IleAsn: 3.609 ± 0.05	3.715IlePro: 3.715 ± 0.046	2.969IleGln: 2.969 ± 0.047	3.128IleArg: 3.128 ± 0.051	5.458IleSer: 5.458 ± 0.071	4.509IleThr: 4.509 ± 0.057	5.65IleVal: 5.65 ± 0.068	0.733IleTrp: 0.733 ± 0.025	2.584IleTyr: 2.584 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	4.831LysAla: 4.831 ± 0.062	0.353LysCys: 0.353 ± 0.015	3.86LysAsp: 3.86 ± 0.054	6.635LysGlu: 6.635 ± 0.071	2.264LysPhe: 2.264 ± 0.038	4.816LysGly: 4.816 ± 0.054	1.292LysHis: 1.292 ± 0.034	5.451LysIle: 5.451 ± 0.07	6.424LysLys: 6.424 ± 0.076	6.146LysLeu: 6.146 ± 0.066	2.391LysMet: 2.391 ± 0.04	3.981LysAsn: 3.981 ± 0.061	2.37LysPro: 2.37 ± 0.039	3.066LysGln: 3.066 ± 0.046	3.196LysArg: 3.196 ± 0.047	3.755LysSer: 3.755 ± 0.051	3.842LysThr: 3.842 ± 0.046	4.965LysVal: 4.965 ± 0.061	0.892LysTrp: 0.892 ± 0.026	2.323LysTyr: 2.323 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	7.092LeuAla: 7.092 ± 0.083	0.724LeuCys: 0.724 ± 0.023	4.591LeuAsp: 4.591 ± 0.063	6.532LeuGlu: 6.532 ± 0.08	5.131LeuPhe: 5.131 ± 0.075	6.633LeuGly: 6.633 ± 0.087	1.844LeuHis: 1.844 ± 0.041	7.816LeuIle: 7.816 ± 0.085	7.23LeuLys: 7.23 ± 0.068	10.194LeuLeu: 10.194 ± 0.092	2.572LeuMet: 2.572 ± 0.042	4.512LeuAsn: 4.512 ± 0.05	3.912LeuPro: 3.912 ± 0.053	3.423LeuGln: 3.423 ± 0.047	3.512LeuArg: 3.512 ± 0.053	6.853LeuSer: 6.853 ± 0.078	5.71LeuThr: 5.71 ± 0.064	6.414LeuVal: 6.414 ± 0.066	0.845LeuTrp: 0.845 ± 0.028	3.105LeuTyr: 3.105 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.052MetAla: 2.052 ± 0.037	0.17MetCys: 0.17 ± 0.012	1.39MetAsp: 1.39 ± 0.029	2.019MetGlu: 2.019 ± 0.041	1.118MetPhe: 1.118 ± 0.028	1.916MetGly: 1.916 ± 0.041	0.398MetHis: 0.398 ± 0.014	2.314MetIle: 2.314 ± 0.042	2.496MetLys: 2.496 ± 0.035	2.457MetLeu: 2.457 ± 0.038	0.836MetMet: 0.836 ± 0.028	1.553MetAsn: 1.553 ± 0.035	1.024MetPro: 1.024 ± 0.027	0.864MetGln: 0.864 ± 0.027	0.99MetArg: 0.99 ± 0.028	1.637MetSer: 1.637 ± 0.034	1.442MetThr: 1.442 ± 0.033	1.896MetVal: 1.896 ± 0.033	0.187MetTrp: 0.187 ± 0.011	0.715MetTyr: 0.715 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.803AsnAla: 2.803 ± 0.048	0.352AsnCys: 0.352 ± 0.017	2.067AsnAsp: 2.067 ± 0.039	3.292AsnGlu: 3.292 ± 0.047	1.913AsnPhe: 1.913 ± 0.034	3.587AsnGly: 3.587 ± 0.052	1.188AsnHis: 1.188 ± 0.028	3.619AsnIle: 3.619 ± 0.046	3.136AsnLys: 3.136 ± 0.051	4.283AsnLeu: 4.283 ± 0.059	1.213AsnMet: 1.213 ± 0.031	2.092AsnAsn: 2.092 ± 0.05	2.331AsnPro: 2.331 ± 0.041	2.132AsnGln: 2.132 ± 0.041	2.115AsnArg: 2.115 ± 0.037	2.555AsnSer: 2.555 ± 0.048	2.207AsnThr: 2.207 ± 0.048	3.036AsnVal: 3.036 ± 0.044	0.575AsnTrp: 0.575 ± 0.02	1.605AsnTyr: 1.605 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	2.326ProAla: 2.326 ± 0.041	0.236ProCys: 0.236 ± 0.01	1.99ProAsp: 1.99 ± 0.037	2.977ProGlu: 2.977 ± 0.045	2.154ProPhe: 2.154 ± 0.041	2.488ProGly: 2.488 ± 0.048	0.773ProHis: 0.773 ± 0.022	3.099ProIle: 3.099 ± 0.048	2.464ProLys: 2.464 ± 0.042	3.501ProLeu: 3.501 ± 0.055	0.864ProMet: 0.864 ± 0.024	1.826ProAsn: 1.826 ± 0.038	1.055ProPro: 1.055 ± 0.031	1.125ProGln: 1.125 ± 0.028	1.11ProArg: 1.11 ± 0.028	2.364ProSer: 2.364 ± 0.046	1.957ProThr: 1.957 ± 0.035	2.88ProVal: 2.88 ± 0.043	0.381ProTrp: 0.381 ± 0.017	1.448ProTyr: 1.448 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	2.511GlnAla: 2.511 ± 0.05	0.204GlnCys: 0.204 ± 0.013	1.524GlnAsp: 1.524 ± 0.031	2.65GlnGlu: 2.65 ± 0.047	1.676GlnPhe: 1.676 ± 0.033	2.106GlnGly: 2.106 ± 0.036	0.7GlnHis: 0.7 ± 0.023	2.725GlnIle: 2.725 ± 0.044	2.91GlnLys: 2.91 ± 0.05	3.777GlnLeu: 3.777 ± 0.053	1.051GlnMet: 1.051 ± 0.028	1.786GlnAsn: 1.786 ± 0.04	1.176GlnPro: 1.176 ± 0.026	1.626GlnGln: 1.626 ± 0.042	1.387GlnArg: 1.387 ± 0.029	2.079GlnSer: 2.079 ± 0.043	1.967GlnThr: 1.967 ± 0.039	2.216GlnVal: 2.216 ± 0.036	0.427GlnTrp: 0.427 ± 0.019	1.359GlnTyr: 1.359 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.337ArgAla: 2.337 ± 0.045	0.245ArgCys: 0.245 ± 0.013	1.884ArgAsp: 1.884 ± 0.042	3.051ArgGlu: 3.051 ± 0.052	1.898ArgPhe: 1.898 ± 0.039	2.286ArgGly: 2.286 ± 0.041	0.806ArgHis: 0.806 ± 0.026	3.128ArgIle: 3.128 ± 0.053	3.242ArgLys: 3.242 ± 0.044	3.775ArgLeu: 3.775 ± 0.047	1.201ArgMet: 1.201 ± 0.029	1.936ArgAsn: 1.936 ± 0.037	1.284ArgPro: 1.284 ± 0.028	1.361ArgGln: 1.361 ± 0.031	1.702ArgArg: 1.702 ± 0.037	2.072ArgSer: 2.072 ± 0.036	1.985ArgThr: 1.985 ± 0.04	2.576ArgVal: 2.576 ± 0.043	0.373ArgTrp: 0.373 ± 0.016	1.436ArgTyr: 1.436 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	3.837SerAla: 3.837 ± 0.056	0.435SerCys: 0.435 ± 0.016	2.615SerAsp: 2.615 ± 0.044	3.705SerGlu: 3.705 ± 0.054	3.513SerPhe: 3.513 ± 0.05	4.615SerGly: 4.615 ± 0.059	1.218SerHis: 1.218 ± 0.03	5.268SerIle: 5.268 ± 0.059	4.084SerLys: 4.084 ± 0.061	6.251SerLeu: 6.251 ± 0.072	1.681SerMet: 1.681 ± 0.036	2.638SerAsn: 2.638 ± 0.045	2.191SerPro: 2.191 ± 0.038	2.036SerGln: 2.036 ± 0.039	2.266SerArg: 2.266 ± 0.038	3.967SerSer: 3.967 ± 0.058	3.085SerThr: 3.085 ± 0.048	4.056SerVal: 4.056 ± 0.06	0.67SerTrp: 0.67 ± 0.023	2.286SerTyr: 2.286 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	3.997ThrAla: 3.997 ± 0.06	0.371ThrCys: 0.371 ± 0.016	2.373ThrAsp: 2.373 ± 0.041	3.208ThrGlu: 3.208 ± 0.045	2.709ThrPhe: 2.709 ± 0.047	4.347ThrGly: 4.347 ± 0.058	0.981ThrHis: 0.981 ± 0.029	4.732ThrIle: 4.732 ± 0.064	3.499ThrLys: 3.499 ± 0.05	5.143ThrLeu: 5.143 ± 0.056	1.265ThrMet: 1.265 ± 0.026	2.561ThrAsn: 2.561 ± 0.048	2.285ThrPro: 2.285 ± 0.039	1.388ThrGln: 1.388 ± 0.031	1.687ThrArg: 1.687 ± 0.033	3.172ThrSer: 3.172 ± 0.051	2.953ThrThr: 2.953 ± 0.058	4.179ThrVal: 4.179 ± 0.054	0.533ThrTrp: 0.533 ± 0.017	1.778ThrTyr: 1.778 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	4.642ValAla: 4.642 ± 0.063	0.594ValCys: 0.594 ± 0.019	3.341ValAsp: 3.341 ± 0.047	4.499ValGlu: 4.499 ± 0.062	3.356ValPhe: 3.356 ± 0.056	4.651ValGly: 4.651 ± 0.062	1.32ValHis: 1.32 ± 0.034	5.85ValIle: 5.85 ± 0.07	4.925ValLys: 4.925 ± 0.059	6.817ValLeu: 6.817 ± 0.08	1.811ValMet: 1.811 ± 0.039	3.217ValAsn: 3.217 ± 0.052	2.748ValPro: 2.748 ± 0.049	2.282ValGln: 2.282 ± 0.044	2.54ValArg: 2.54 ± 0.048	4.543ValSer: 4.543 ± 0.055	4.102ValThr: 4.102 ± 0.064	4.865ValVal: 4.865 ± 0.064	0.66ValTrp: 0.66 ± 0.022	2.256ValTyr: 2.256 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.652TrpAla: 0.652 ± 0.021	0.088TrpCys: 0.088 ± 0.007	0.545TrpAsp: 0.545 ± 0.02	0.684TrpGlu: 0.684 ± 0.023	0.609TrpPhe: 0.609 ± 0.024	0.705TrpGly: 0.705 ± 0.023	0.204TrpHis: 0.204 ± 0.012	0.887TrpIle: 0.887 ± 0.03	0.811TrpLys: 0.811 ± 0.023	1.151TrpLeu: 1.151 ± 0.031	0.344TrpMet: 0.344 ± 0.014	0.551TrpAsn: 0.551 ± 0.022	0.269TrpPro: 0.269 ± 0.013	0.325TrpGln: 0.325 ± 0.018	0.397TrpArg: 0.397 ± 0.016	0.599TrpSer: 0.599 ± 0.018	0.479TrpThr: 0.479 ± 0.02	0.726TrpVal: 0.726 ± 0.023	0.146TrpTrp: 0.146 ± 0.01	0.386TrpTyr: 0.386 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.019TyrAla: 2.019 ± 0.038	0.329TyrCys: 0.329 ± 0.015	1.774TyrAsp: 1.774 ± 0.046	2.272TyrGlu: 2.272 ± 0.042	1.939TyrPhe: 1.939 ± 0.044	2.565TyrGly: 2.565 ± 0.041	0.885TyrHis: 0.885 ± 0.025	2.522TyrIle: 2.522 ± 0.041	2.199TyrLys: 2.199 ± 0.044	3.617TyrLeu: 3.617 ± 0.053	0.808TyrMet: 0.808 ± 0.022	1.498TyrAsn: 1.498 ± 0.033	1.469TyrPro: 1.469 ± 0.032	1.55TyrGln: 1.55 ± 0.037	1.594TyrArg: 1.594 ± 0.032	2.217TyrSer: 2.217 ± 0.037	1.798TyrThr: 1.798 ± 0.04	2.123TyrVal: 2.123 ± 0.034	0.429TyrTrp: 0.429 ± 0.018	1.418TyrTyr: 1.418 ± 0.035	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5271 proteins (1518309 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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