Methylovulum psychrotolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylovulum

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z4C3I1|A0A1Z4C3I1_9GAMM Tyrosinase OS=Methylovulum psychrotolerans OX=1704499 GN=CEK71_19595 PE=4 SV=1
MM1 pKa = 7.54SNTAPSFITKK11 pKa = 9.86NDD13 pKa = 4.75PITTDD18 pKa = 4.2FGYY21 pKa = 10.62SDD23 pKa = 3.33QAYY26 pKa = 9.39DD27 pKa = 4.3VSVQTDD33 pKa = 3.51GKK35 pKa = 10.49LVISGTVYY43 pKa = 11.11LDD45 pKa = 3.0MMADD49 pKa = 3.58MGQGLMGSSVLRR61 pKa = 11.84YY62 pKa = 10.12NSDD65 pKa = 2.9GSLDD69 pKa = 3.74LSFSGDD75 pKa = 3.09GKK77 pKa = 11.0LDD79 pKa = 3.47TRR81 pKa = 11.84GLIHH85 pKa = 6.94AQALQADD92 pKa = 4.6GKK94 pKa = 10.65ILVAGTASTSFDD106 pKa = 3.88FYY108 pKa = 11.52DD109 pKa = 4.6FDD111 pKa = 3.91IEE113 pKa = 4.71RR114 pKa = 11.84YY115 pKa = 9.99NSDD118 pKa = 3.0GSLDD122 pKa = 3.6TTFGNGGVVTTNFLLSNDD140 pKa = 3.21IAYY143 pKa = 10.49SIAVQSDD150 pKa = 3.21GKK152 pKa = 10.49ILAAGKK158 pKa = 9.97SGHH161 pKa = 7.29DD162 pKa = 3.76YY163 pKa = 11.59GLVRR167 pKa = 11.84YY168 pKa = 9.02NSNGSLDD175 pKa = 3.62TSFSGDD181 pKa = 3.05GKK183 pKa = 10.86VATDD187 pKa = 3.41MGADD191 pKa = 4.17FEE193 pKa = 4.53QANSIVLQSNGKK205 pKa = 8.9IVLGGSSGLVRR216 pKa = 11.84YY217 pKa = 7.73NTDD220 pKa = 2.57GSLDD224 pKa = 3.51TGFSGDD230 pKa = 3.9GKK232 pKa = 10.7LSTSGITNLALQTDD246 pKa = 4.73GKK248 pKa = 10.94LLATEE253 pKa = 4.56ADD255 pKa = 3.85GTLDD259 pKa = 3.98RR260 pKa = 11.84YY261 pKa = 11.08NSDD264 pKa = 3.01GSVDD268 pKa = 3.46TSFSGDD274 pKa = 3.23GKK276 pKa = 9.95VTDD279 pKa = 3.39VWANTLLLQSDD290 pKa = 4.42GKK292 pKa = 11.32LLTTNNYY299 pKa = 10.13SEE301 pKa = 4.24LTRR304 pKa = 11.84YY305 pKa = 10.21NSDD308 pKa = 2.93GSLDD312 pKa = 3.59TSFSGDD318 pKa = 3.1GKK320 pKa = 9.12ITIGFTINATTVQADD335 pKa = 3.56GKK337 pKa = 10.54IVVTGYY343 pKa = 10.87GFNYY347 pKa = 8.0PQAYY351 pKa = 9.7LSYY354 pKa = 10.17YY355 pKa = 10.41RR356 pKa = 11.84YY357 pKa = 10.63DD358 pKa = 3.87FYY360 pKa = 10.9TARR363 pKa = 11.84YY364 pKa = 8.91NSDD367 pKa = 2.84GSLDD371 pKa = 3.65TTFTQSNDD379 pKa = 3.24SLRR382 pKa = 11.84LPLSITEE389 pKa = 4.45NDD391 pKa = 3.47PATYY395 pKa = 9.99LAEE398 pKa = 4.2LAQIRR403 pKa = 11.84DD404 pKa = 3.86TEE406 pKa = 4.2LDD408 pKa = 3.46ALNGGLGDD416 pKa = 3.68YY417 pKa = 10.75SGASLTLARR426 pKa = 11.84HH427 pKa = 6.09GGANTDD433 pKa = 3.8DD434 pKa = 3.76VFGGSGVLALSNGAITVSGVNIGTYY459 pKa = 7.39QQSGGQLTLTFGAATTQQVNSALQHH484 pKa = 5.21LTYY487 pKa = 10.83QNTSDD492 pKa = 4.05LTPPSLQIDD501 pKa = 3.54WTFSDD506 pKa = 4.46GALSTVGNTTVDD518 pKa = 3.17ISPQYY523 pKa = 11.04DD524 pKa = 3.25ATTGYY529 pKa = 11.88AMINGSRR536 pKa = 11.84TVGQTLTTSNNFTDD550 pKa = 4.07PDD552 pKa = 3.93GLGTISYY559 pKa = 9.0QWQGSSNGTTWSNLGSGPTLVVTQAMLSQQIRR591 pKa = 11.84LTDD594 pKa = 3.3SYY596 pKa = 11.45TDD598 pKa = 3.25QQGAAKK604 pKa = 10.41SITTVQGTSGNDD616 pKa = 3.24NILSGDD622 pKa = 3.71AKK624 pKa = 10.79FGMDD628 pKa = 4.72GNDD631 pKa = 3.14HH632 pKa = 6.93LYY634 pKa = 10.54SYY636 pKa = 11.04SSTYY640 pKa = 10.05GHH642 pKa = 6.53SNSILMDD649 pKa = 4.07GGNGKK654 pKa = 9.8DD655 pKa = 3.46VVDD658 pKa = 4.42YY659 pKa = 11.39SPADD663 pKa = 3.34ILNVGVTVDD672 pKa = 3.92LTLTTAQNTVAVGMNTLLNIEE693 pKa = 4.65DD694 pKa = 5.67LIGTLNNDD702 pKa = 3.44TFTGNALANSLFGQAGNDD720 pKa = 3.66TLNGGGGNDD729 pKa = 4.05GLTGGAGDD737 pKa = 4.61DD738 pKa = 4.04RR739 pKa = 11.84LDD741 pKa = 3.92GGTGTDD747 pKa = 2.94MAYY750 pKa = 10.72YY751 pKa = 10.19NSAATGVTVNLALTTAQNTHH771 pKa = 5.72GAGTDD776 pKa = 3.18TLLNIEE782 pKa = 4.54SLNGSTYY789 pKa = 11.14NDD791 pKa = 3.3VLVGNYY797 pKa = 10.24ANNTLLGGNGNDD809 pKa = 3.4VLAGSLGNDD818 pKa = 3.54TLTGGAGKK826 pKa = 10.2DD827 pKa = 2.69IFAFNSALGINNIDD841 pKa = 3.59TLTDD845 pKa = 3.58FNVADD850 pKa = 3.6DD851 pKa = 4.9TIRR854 pKa = 11.84LEE856 pKa = 4.07NGIFTTLTTPGVLAAEE872 pKa = 4.21AFKK875 pKa = 10.65IIGNGGVADD884 pKa = 4.05STDD887 pKa = 3.0HH888 pKa = 6.96LLYY891 pKa = 9.91NTATGALSYY900 pKa = 11.0DD901 pKa = 3.68ADD903 pKa = 3.99GSGAAAAVQIAILGTGLAMTNADD926 pKa = 4.33FIVVV930 pKa = 3.78

Molecular weight: 96.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z4BVX5|A0A1Z4BVX5_9GAMM Uncharacterized protein OS=Methylovulum psychrotolerans OX=1704499 GN=CEK71_04840 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 8.35IKK11 pKa = 10.26RR12 pKa = 11.84VRR14 pKa = 11.84THH16 pKa = 5.82GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.1VLNSRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84HH40 pKa = 5.12KK41 pKa = 10.72LALL44 pKa = 3.92

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4299

0

4299

1384159

18

3606

322.0

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.419 ± 0.049

1.07 ± 0.013

5.539 ± 0.032

5.263 ± 0.048

3.94 ± 0.024

7.265 ± 0.054

2.364 ± 0.021

5.76 ± 0.032

4.634 ± 0.04

11.174 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.017

3.945 ± 0.032

4.578 ± 0.032

4.741 ± 0.034

4.883 ± 0.035

5.73 ± 0.034

5.554 ± 0.038

6.57 ± 0.03

1.33 ± 0.018

3.095 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski