Amino acid dipepetide frequency for Cyprinodon variegatus (Sheepshead minnow)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.223AlaAla: 6.223 ± 0.031	1.231AlaCys: 1.231 ± 0.011	3.088AlaAsp: 3.088 ± 0.014	4.589AlaGlu: 4.589 ± 0.024	2.411AlaPhe: 2.411 ± 0.015	4.214AlaGly: 4.214 ± 0.021	1.407AlaHis: 1.407 ± 0.009	2.712AlaIle: 2.712 ± 0.013	3.32AlaLys: 3.32 ± 0.019	6.34AlaLeu: 6.34 ± 0.026	1.544AlaMet: 1.544 ± 0.011	2.177AlaAsn: 2.177 ± 0.011	3.471AlaPro: 3.471 ± 0.019	2.837AlaGln: 2.837 ± 0.016	2.941AlaArg: 2.941 ± 0.015	5.405AlaSer: 5.405 ± 0.022	3.295AlaThr: 3.295 ± 0.016	4.75AlaVal: 4.75 ± 0.019	0.637AlaTrp: 0.637 ± 0.006	1.508AlaTyr: 1.508 ± 0.01	0.0AlaXaa: 0.0 ± 0.0
Cys	1.12CysAla: 1.12 ± 0.01	0.675CysCys: 0.675 ± 0.009	1.108CysAsp: 1.108 ± 0.012	1.248CysGlu: 1.248 ± 0.011	0.978CysPhe: 0.978 ± 0.008	1.558CysGly: 1.558 ± 0.015	0.622CysHis: 0.622 ± 0.007	1.018CysIle: 1.018 ± 0.009	1.198CysLys: 1.198 ± 0.01	2.181CysLeu: 2.181 ± 0.013	0.477CysMet: 0.477 ± 0.006	0.877CysAsn: 0.877 ± 0.008	1.27CysPro: 1.27 ± 0.012	1.004CysGln: 1.004 ± 0.011	1.266CysArg: 1.266 ± 0.011	2.223CysSer: 2.223 ± 0.015	1.149CysThr: 1.149 ± 0.011	1.427CysVal: 1.427 ± 0.011	0.297CysTrp: 0.297 ± 0.004	0.627CysTyr: 0.627 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	2.882AspAla: 2.882 ± 0.014	1.135AspCys: 1.135 ± 0.011	2.983AspAsp: 2.983 ± 0.021	3.694AspGlu: 3.694 ± 0.017	2.159AspPhe: 2.159 ± 0.013	3.656AspGly: 3.656 ± 0.018	1.171AspHis: 1.171 ± 0.008	2.668AspIle: 2.668 ± 0.015	2.676AspLys: 2.676 ± 0.015	5.023AspLeu: 5.023 ± 0.02	1.263AspMet: 1.263 ± 0.01	1.911AspAsn: 1.911 ± 0.01	2.905AspPro: 2.905 ± 0.014	1.983AspGln: 1.983 ± 0.012	2.679AspArg: 2.679 ± 0.014	4.443AspSer: 4.443 ± 0.019	2.444AspThr: 2.444 ± 0.013	3.218AspVal: 3.218 ± 0.015	0.657AspTrp: 0.657 ± 0.007	1.548AspTyr: 1.548 ± 0.01	0.0AspXaa: 0.0 ± 0.0
Glu	4.664GluAla: 4.664 ± 0.024	1.184GluCys: 1.184 ± 0.014	4.375GluAsp: 4.375 ± 0.021	7.728GluGlu: 7.728 ± 0.052	1.962GluPhe: 1.962 ± 0.011	4.053GluGly: 4.053 ± 0.019	1.428GluHis: 1.428 ± 0.01	2.968GluIle: 2.968 ± 0.016	4.967GluLys: 4.967 ± 0.03	6.091GluLeu: 6.091 ± 0.032	1.734GluMet: 1.734 ± 0.012	2.94GluAsn: 2.94 ± 0.017	2.821GluPro: 2.821 ± 0.017	3.082GluGln: 3.082 ± 0.018	4.193GluArg: 4.193 ± 0.024	4.416GluSer: 4.416 ± 0.02	3.453GluThr: 3.453 ± 0.018	4.203GluVal: 4.203 ± 0.018	0.682GluTrp: 0.682 ± 0.006	1.572GluTyr: 1.572 ± 0.011	0.001GluXaa: 0.001 ± 0.0
Phe	1.935PheAla: 1.935 ± 0.012	1.012PheCys: 1.012 ± 0.008	1.76PheAsp: 1.76 ± 0.01	1.879PheGlu: 1.879 ± 0.013	1.812PhePhe: 1.812 ± 0.014	2.231PheGly: 2.231 ± 0.013	1.045PheHis: 1.045 ± 0.008	2.07PheIle: 2.07 ± 0.012	1.924PheLys: 1.924 ± 0.012	4.089PheLeu: 4.089 ± 0.021	0.846PheMet: 0.846 ± 0.007	1.565PheAsn: 1.565 ± 0.01	1.931PhePro: 1.931 ± 0.012	1.692PheGln: 1.692 ± 0.01	1.887PheArg: 1.887 ± 0.011	3.663PheSer: 3.663 ± 0.019	2.345PheThr: 2.345 ± 0.014	2.243PheVal: 2.243 ± 0.014	0.49PheTrp: 0.49 ± 0.006	1.286PheTyr: 1.286 ± 0.009	0.0PheXaa: 0.0 ± 0.0
Gly	3.939GlyAla: 3.939 ± 0.021	1.266GlyCys: 1.266 ± 0.011	3.141GlyAsp: 3.141 ± 0.017	4.129GlyGlu: 4.129 ± 0.027	2.502GlyPhe: 2.502 ± 0.015	5.196GlyGly: 5.196 ± 0.031	1.607GlyHis: 1.607 ± 0.011	2.639GlyIle: 2.639 ± 0.016	3.657GlyLys: 3.657 ± 0.018	5.417GlyLeu: 5.417 ± 0.023	1.447GlyMet: 1.447 ± 0.01	2.528GlyAsn: 2.528 ± 0.015	3.318GlyPro: 3.318 ± 0.041	2.701GlyGln: 2.701 ± 0.018	3.558GlyArg: 3.558 ± 0.021	5.845GlySer: 5.845 ± 0.025	3.304GlyThr: 3.304 ± 0.015	3.882GlyVal: 3.882 ± 0.016	0.763GlyTrp: 0.763 ± 0.008	1.838GlyTyr: 1.838 ± 0.012	0.001GlyXaa: 0.001 ± 0.0
His	1.262HisAla: 1.262 ± 0.009	0.723HisCys: 0.723 ± 0.007	0.939HisAsp: 0.939 ± 0.009	1.175HisGlu: 1.175 ± 0.011	1.066HisPhe: 1.066 ± 0.008	1.509HisGly: 1.509 ± 0.011	1.029HisHis: 1.029 ± 0.012	1.34HisIle: 1.34 ± 0.01	1.287HisLys: 1.287 ± 0.009	2.722HisLeu: 2.722 ± 0.014	0.7HisMet: 0.7 ± 0.008	1.016HisAsn: 1.016 ± 0.009	1.61HisPro: 1.61 ± 0.012	1.283HisGln: 1.283 ± 0.01	1.627HisArg: 1.627 ± 0.01	2.424HisSer: 2.424 ± 0.016	1.523HisThr: 1.523 ± 0.014	1.401HisVal: 1.401 ± 0.009	0.33HisTrp: 0.33 ± 0.004	0.844HisTyr: 0.844 ± 0.008	0.0HisXaa: 0.0 ± 0.0
Ile	2.491IleAla: 2.491 ± 0.014	1.158IleCys: 1.158 ± 0.01	2.109IleAsp: 2.109 ± 0.012	2.46IleGlu: 2.46 ± 0.015	1.946IlePhe: 1.946 ± 0.015	2.346IleGly: 2.346 ± 0.014	1.325IleHis: 1.325 ± 0.01	2.567IleIle: 2.567 ± 0.018	2.71IleLys: 2.71 ± 0.014	4.421IleLeu: 4.421 ± 0.018	1.08IleMet: 1.08 ± 0.009	2.083IleAsn: 2.083 ± 0.013	2.544IlePro: 2.544 ± 0.014	2.255IleGln: 2.255 ± 0.012	2.561IleArg: 2.561 ± 0.014	4.007IleSer: 4.007 ± 0.014	2.845IleThr: 2.845 ± 0.017	2.576IleVal: 2.576 ± 0.015	0.499IleTrp: 0.499 ± 0.006	1.483IleTyr: 1.483 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	3.796LysAla: 3.796 ± 0.019	1.058LysCys: 1.058 ± 0.01	3.296LysAsp: 3.296 ± 0.018	4.92LysGlu: 4.92 ± 0.03	1.627LysPhe: 1.627 ± 0.01	3.303LysGly: 3.303 ± 0.019	1.449LysHis: 1.449 ± 0.01	2.613LysIle: 2.613 ± 0.014	4.6LysLys: 4.6 ± 0.029	5.089LysLeu: 5.089 ± 0.024	1.48LysMet: 1.48 ± 0.011	2.44LysAsn: 2.44 ± 0.014	3.087LysPro: 3.087 ± 0.019	2.64LysGln: 2.64 ± 0.015	3.434LysArg: 3.434 ± 0.018	3.975LysSer: 3.975 ± 0.019	3.292LysThr: 3.292 ± 0.016	3.59LysVal: 3.59 ± 0.016	0.586LysTrp: 0.586 ± 0.006	1.529LysTyr: 1.529 ± 0.011	0.001LysXaa: 0.001 ± 0.0
Leu	5.809LeuAla: 5.809 ± 0.023	2.238LeuCys: 2.238 ± 0.015	4.871LeuAsp: 4.871 ± 0.021	6.402LeuGlu: 6.402 ± 0.029	3.557LeuPhe: 3.557 ± 0.018	5.066LeuGly: 5.066 ± 0.02	2.699LeuHis: 2.699 ± 0.015	4.031LeuIle: 4.031 ± 0.021	5.847LeuLys: 5.847 ± 0.026	10.144LeuLeu: 10.144 ± 0.037	2.173LeuMet: 2.173 ± 0.012	3.785LeuAsn: 3.785 ± 0.015	5.347LeuPro: 5.347 ± 0.022	5.503LeuGln: 5.503 ± 0.027	5.56LeuArg: 5.56 ± 0.021	8.461LeuSer: 8.461 ± 0.029	5.267LeuThr: 5.267 ± 0.021	5.342LeuVal: 5.342 ± 0.022	1.05LeuTrp: 1.05 ± 0.009	2.639LeuTyr: 2.639 ± 0.016	0.001LeuXaa: 0.001 ± 0.0
Met	1.876MetAla: 1.876 ± 0.01	0.476MetCys: 0.476 ± 0.006	1.421MetAsp: 1.421 ± 0.009	2.02MetGlu: 2.02 ± 0.012	0.894MetPhe: 0.894 ± 0.008	1.412MetGly: 1.412 ± 0.011	0.494MetHis: 0.494 ± 0.005	0.93MetIle: 0.93 ± 0.007	1.524MetLys: 1.524 ± 0.011	2.098MetLeu: 2.098 ± 0.011	0.728MetMet: 0.728 ± 0.008	0.933MetAsn: 0.933 ± 0.008	1.074MetPro: 1.074 ± 0.009	1.007MetGln: 1.007 ± 0.008	1.222MetArg: 1.222 ± 0.008	1.887MetSer: 1.887 ± 0.011	1.256MetThr: 1.256 ± 0.009	1.521MetVal: 1.521 ± 0.01	0.259MetTrp: 0.259 ± 0.004	0.627MetTyr: 0.627 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.181AsnAla: 2.181 ± 0.012	0.883AsnCys: 0.883 ± 0.009	1.717AsnAsp: 1.717 ± 0.012	2.145AsnGlu: 2.145 ± 0.013	1.491AsnPhe: 1.491 ± 0.011	2.838AsnGly: 2.838 ± 0.019	1.044AsnHis: 1.044 ± 0.009	2.28AsnIle: 2.28 ± 0.014	2.384AsnLys: 2.384 ± 0.013	3.837AsnLeu: 3.837 ± 0.018	1.092AsnMet: 1.092 ± 0.008	1.895AsnAsn: 1.895 ± 0.013	2.348AsnPro: 2.348 ± 0.015	1.887AsnGln: 1.887 ± 0.012	2.092AsnArg: 2.092 ± 0.013	3.391AsnSer: 3.391 ± 0.015	2.266AsnThr: 2.266 ± 0.013	2.327AsnVal: 2.327 ± 0.014	0.45AsnTrp: 0.45 ± 0.005	1.183AsnTyr: 1.183 ± 0.01	0.001AsnXaa: 0.001 ± 0.0
Pro	4.07ProAla: 4.07 ± 0.02	1.082ProCys: 1.082 ± 0.013	2.845ProAsp: 2.845 ± 0.015	3.757ProGlu: 3.757 ± 0.019	1.937ProPhe: 1.937 ± 0.015	4.072ProGly: 4.072 ± 0.046	1.454ProHis: 1.454 ± 0.011	1.992ProIle: 1.992 ± 0.011	2.646ProLys: 2.646 ± 0.015	4.932ProLeu: 4.932 ± 0.02	1.045ProMet: 1.045 ± 0.008	2.051ProAsn: 2.051 ± 0.013	5.523ProPro: 5.523 ± 0.046	2.681ProGln: 2.681 ± 0.017	2.698ProArg: 2.698 ± 0.016	5.744ProSer: 5.744 ± 0.033	3.111ProThr: 3.111 ± 0.02	3.715ProVal: 3.715 ± 0.018	0.543ProTrp: 0.543 ± 0.006	1.442ProTyr: 1.442 ± 0.01	0.001ProXaa: 0.001 ± 0.0
Gln	3.076GlnAla: 3.076 ± 0.017	0.924GlnCys: 0.924 ± 0.01	2.26GlnAsp: 2.26 ± 0.012	3.514GlnGlu: 3.514 ± 0.02	1.368GlnPhe: 1.368 ± 0.01	2.706GlnGly: 2.706 ± 0.019	1.323GlnHis: 1.323 ± 0.011	2.02GlnIle: 2.02 ± 0.01	2.717GlnLys: 2.717 ± 0.017	4.462GlnLeu: 4.462 ± 0.022	1.175GlnMet: 1.175 ± 0.009	1.921GlnAsn: 1.921 ± 0.01	2.592GlnPro: 2.592 ± 0.018	3.336GlnGln: 3.336 ± 0.039	3.09GlnArg: 3.09 ± 0.02	3.573GlnSer: 3.573 ± 0.019	2.638GlnThr: 2.638 ± 0.018	2.745GlnVal: 2.745 ± 0.015	0.531GlnTrp: 0.531 ± 0.006	1.21GlnTyr: 1.21 ± 0.01	0.001GlnXaa: 0.001 ± 0.0
Arg	3.269ArgAla: 3.269 ± 0.016	1.234ArgCys: 1.234 ± 0.011	2.847ArgAsp: 2.847 ± 0.016	3.821ArgGlu: 3.821 ± 0.022	2.018ArgPhe: 2.018 ± 0.013	3.385ArgGly: 3.385 ± 0.022	1.545ArgHis: 1.545 ± 0.011	2.474ArgIle: 2.474 ± 0.014	3.592ArgLys: 3.592 ± 0.017	5.235ArgLeu: 5.235 ± 0.024	1.296ArgMet: 1.296 ± 0.009	2.17ArgAsn: 2.17 ± 0.011	2.896ArgPro: 2.896 ± 0.014	2.598ArgGln: 2.598 ± 0.015	4.318ArgArg: 4.318 ± 0.025	4.628ArgSer: 4.628 ± 0.024	2.977ArgThr: 2.977 ± 0.016	3.165ArgVal: 3.165 ± 0.016	0.666ArgTrp: 0.666 ± 0.007	1.575ArgTyr: 1.575 ± 0.012	0.001ArgXaa: 0.001 ± 0.0
Ser	5.609SerAla: 5.609 ± 0.021	2.071SerCys: 2.071 ± 0.016	4.371SerAsp: 4.371 ± 0.02	5.047SerGlu: 5.047 ± 0.022	3.335SerPhe: 3.335 ± 0.017	5.626SerGly: 5.626 ± 0.025	2.222SerHis: 2.222 ± 0.015	3.525SerIle: 3.525 ± 0.015	4.22SerLys: 4.22 ± 0.021	8.483SerLeu: 8.483 ± 0.028	1.843SerMet: 1.843 ± 0.01	3.225SerAsn: 3.225 ± 0.015	6.035SerPro: 6.035 ± 0.034	3.933SerGln: 3.933 ± 0.021	4.59SerArg: 4.59 ± 0.02	10.711SerSer: 10.711 ± 0.058	4.793SerThr: 4.793 ± 0.024	5.367SerVal: 5.367 ± 0.02	1.03SerTrp: 1.03 ± 0.008	2.258SerTyr: 2.258 ± 0.013	0.001SerXaa: 0.001 ± 0.0
Thr	3.902ThrAla: 3.902 ± 0.015	1.354ThrCys: 1.354 ± 0.013	2.825ThrAsp: 2.825 ± 0.014	3.687ThrGlu: 3.687 ± 0.019	2.2ThrPhe: 2.2 ± 0.014	3.719ThrGly: 3.719 ± 0.02	1.354ThrHis: 1.354 ± 0.011	2.491ThrIle: 2.491 ± 0.014	2.742ThrLys: 2.742 ± 0.014	5.297ThrLeu: 5.297 ± 0.019	1.202ThrMet: 1.202 ± 0.009	2.024ThrAsn: 2.024 ± 0.013	3.564ThrPro: 3.564 ± 0.024	2.313ThrGln: 2.313 ± 0.014	2.422ThrArg: 2.422 ± 0.014	4.869ThrSer: 4.869 ± 0.023	3.161ThrThr: 3.161 ± 0.024	4.01ThrVal: 4.01 ± 0.022	0.663ThrTrp: 0.663 ± 0.008	1.435ThrTyr: 1.435 ± 0.01	0.001ThrXaa: 0.001 ± 0.0
Val	3.917ValAla: 3.917 ± 0.02	1.647ValCys: 1.647 ± 0.01	3.121ValAsp: 3.121 ± 0.015	3.956ValGlu: 3.956 ± 0.018	2.691ValPhe: 2.691 ± 0.016	3.49ValGly: 3.49 ± 0.017	1.524ValHis: 1.524 ± 0.01	2.996ValIle: 2.996 ± 0.016	3.555ValLys: 3.555 ± 0.018	6.129ValLeu: 6.129 ± 0.023	1.5ValMet: 1.5 ± 0.009	2.426ValAsn: 2.426 ± 0.013	3.234ValPro: 3.234 ± 0.018	2.722ValGln: 2.722 ± 0.014	3.164ValArg: 3.164 ± 0.015	5.286ValSer: 5.286 ± 0.024	3.815ValThr: 3.815 ± 0.018	4.197ValVal: 4.197 ± 0.021	0.767ValTrp: 0.767 ± 0.007	1.795ValTyr: 1.795 ± 0.012	0.001ValXaa: 0.001 ± 0.0
Trp	0.646TrpAla: 0.646 ± 0.006	0.241TrpCys: 0.241 ± 0.004	0.626TrpAsp: 0.626 ± 0.007	0.752TrpGlu: 0.752 ± 0.007	0.489TrpPhe: 0.489 ± 0.006	0.611TrpGly: 0.611 ± 0.007	0.273TrpHis: 0.273 ± 0.005	0.597TrpIle: 0.597 ± 0.006	0.732TrpLys: 0.732 ± 0.007	1.127TrpLeu: 1.127 ± 0.009	0.356TrpMet: 0.356 ± 0.004	0.528TrpAsn: 0.528 ± 0.006	0.425TrpPro: 0.425 ± 0.005	0.47TrpGln: 0.47 ± 0.005	0.725TrpArg: 0.725 ± 0.007	0.955TrpSer: 0.955 ± 0.009	0.705TrpThr: 0.705 ± 0.008	0.683TrpVal: 0.683 ± 0.006	0.189TrpTrp: 0.189 ± 0.003	0.346TrpTyr: 0.346 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.43TyrAla: 1.43 ± 0.01	0.732TyrCys: 0.732 ± 0.007	1.398TyrAsp: 1.398 ± 0.009	1.594TyrGlu: 1.594 ± 0.009	1.26TyrPhe: 1.26 ± 0.01	1.685TyrGly: 1.685 ± 0.011	0.797TyrHis: 0.797 ± 0.007	1.538TyrIle: 1.538 ± 0.01	1.508TyrLys: 1.508 ± 0.011	2.673TyrLeu: 2.673 ± 0.014	0.679TyrMet: 0.679 ± 0.006	1.234TyrAsn: 1.234 ± 0.009	1.345TyrPro: 1.345 ± 0.01	1.256TyrGln: 1.256 ± 0.009	1.681TyrArg: 1.681 ± 0.01	2.387TyrSer: 2.387 ± 0.012	1.592TyrThr: 1.592 ± 0.012	1.569TyrVal: 1.569 ± 0.011	0.389TyrTrp: 0.389 ± 0.006	0.996TyrTyr: 0.996 ± 0.009	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.392XaaXaa: 0.392 ± 0.077

Statistics based on 31881 proteins (17389895 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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