Capybara microvirus Cap3_SP_546

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6C6|A0A4P8W6C6_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_546 OX=2585473 PE=4 SV=1
MM1 pKa = 7.42FRR3 pKa = 11.84TKK5 pKa = 10.63YY6 pKa = 10.49NCDD9 pKa = 3.15NSSVVSNNGSPFIDD23 pKa = 3.39TYY25 pKa = 11.78GFTTDD30 pKa = 4.91DD31 pKa = 3.65YY32 pKa = 11.0GNTIVGVTGQSNLFDD47 pKa = 5.52IIQSNSDD54 pKa = 3.33SGNISLIVDD63 pKa = 3.99RR64 pKa = 11.84ATRR67 pKa = 11.84GDD69 pKa = 3.99LSYY72 pKa = 11.67LNAVNSAYY80 pKa = 10.49FDD82 pKa = 4.03ARR84 pKa = 11.84DD85 pKa = 3.46LPTSYY90 pKa = 11.01RR91 pKa = 11.84EE92 pKa = 3.49MTEE95 pKa = 4.45YY96 pKa = 10.48INHH99 pKa = 6.7CNNTFNEE106 pKa = 4.29LPAHH110 pKa = 6.22IKK112 pKa = 9.95EE113 pKa = 4.33AFNNSSSEE121 pKa = 4.0FWSTFGSDD129 pKa = 2.25SWNRR133 pKa = 11.84RR134 pKa = 11.84VGFNSEE140 pKa = 4.44DD141 pKa = 3.49SSSNDD146 pKa = 2.81GGDD149 pKa = 3.63FNEE152 pKa = 4.56QEE154 pKa = 4.07

Molecular weight: 17.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W8U1|A0A4P8W8U1_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_546 OX=2585473 PE=3 SV=1
MM1 pKa = 7.36SAYY4 pKa = 9.44TEE6 pKa = 4.22ALQLQRR12 pKa = 11.84DD13 pKa = 3.89AQSFTKK19 pKa = 9.66QQWNFEE25 pKa = 4.33QNLSSTAHH33 pKa = 4.86QRR35 pKa = 11.84EE36 pKa = 4.02VSDD39 pKa = 4.25LKK41 pKa = 11.07KK42 pKa = 10.88AGLNPVLSANSGASTPSVGLDD63 pKa = 3.11SGVQAASGYY72 pKa = 9.06MSSKK76 pKa = 8.64TAAAATRR83 pKa = 11.84YY84 pKa = 9.19ASNMSYY90 pKa = 10.81KK91 pKa = 10.49AALAQASATRR101 pKa = 11.84YY102 pKa = 8.86AASLNYY108 pKa = 8.26EE109 pKa = 4.13AQMSSNKK116 pKa = 10.16YY117 pKa = 9.37GIAMQIANSAAGEE130 pKa = 4.16GGVIGFIKK138 pKa = 10.34RR139 pKa = 11.84AVNSAKK145 pKa = 10.38SYY147 pKa = 11.0NSSIDD152 pKa = 3.41NTVKK156 pKa = 10.77SNNISIPNVKK166 pKa = 8.45VTPQSVLNGKK176 pKa = 8.9ALNGVKK182 pKa = 10.11RR183 pKa = 11.84EE184 pKa = 4.14DD185 pKa = 3.48LLKK188 pKa = 10.46PVTSSSQYY196 pKa = 9.7QFGNKK201 pKa = 6.86VTLNDD206 pKa = 3.32TGKK209 pKa = 10.59KK210 pKa = 9.33YY211 pKa = 10.63VSKK214 pKa = 10.73VLSDD218 pKa = 4.01LGVSTKK224 pKa = 10.87APIGKK229 pKa = 8.78EE230 pKa = 3.82CQIYY234 pKa = 8.88LQQYY238 pKa = 9.52LITGNLVYY246 pKa = 10.82YY247 pKa = 10.53RR248 pKa = 11.84GFQRR252 pKa = 11.84SVSTYY257 pKa = 9.88LNTKK261 pKa = 8.96KK262 pKa = 10.23RR263 pKa = 11.84YY264 pKa = 8.89PYY266 pKa = 10.24ISS268 pKa = 3.23

Molecular weight: 28.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1423

80

565

284.6

32.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.833 ± 1.055

1.546 ± 0.4

6.676 ± 0.828

4.076 ± 0.538

4.638 ± 0.649

6.184 ± 0.236

1.476 ± 0.251

6.325 ± 0.561

5.341 ± 1.304

7.238 ± 0.691

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.319 ± 0.486

7.449 ± 1.133

4.216 ± 0.691

3.584 ± 0.631

4.989 ± 0.591

10.049 ± 1.509

5.411 ± 0.335

5.13 ± 1.05

1.405 ± 0.277

6.114 ± 0.998
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski