Amino acid dipepetide frequency for Mycobacterium phage Keziacharles14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.966AlaAla: 11.966 ± 1.251
0.541AlaCys: 0.541 ± 0.202
5.472AlaAsp: 5.472 ± 0.651
7.156AlaGlu: 7.156 ± 0.716
3.067AlaPhe: 3.067 ± 0.514
7.937AlaGly: 7.937 ± 0.652
1.924AlaHis: 1.924 ± 0.333
4.931AlaIle: 4.931 ± 0.469
5.953AlaLys: 5.953 ± 0.764
8.9AlaLeu: 8.9 ± 0.953
3.127AlaMet: 3.127 ± 0.507
3.608AlaAsn: 3.608 ± 0.577
4.57AlaPro: 4.57 ± 0.932
3.728AlaGln: 3.728 ± 0.659
6.254AlaArg: 6.254 ± 0.748
5.592AlaSer: 5.592 ± 0.543
6.374AlaThr: 6.374 ± 0.533
6.915AlaVal: 6.915 ± 0.647
1.624AlaTrp: 1.624 ± 0.322
2.826AlaTyr: 2.826 ± 0.539
0.0AlaXaa: 0.0 ± 0.0
Cys
0.722CysAla: 0.722 ± 0.221
0.12CysCys: 0.12 ± 0.132
0.541CysAsp: 0.541 ± 0.217
0.541CysGlu: 0.541 ± 0.204
0.301CysPhe: 0.301 ± 0.129
0.722CysGly: 0.722 ± 0.208
0.18CysHis: 0.18 ± 0.103
0.301CysIle: 0.301 ± 0.12
0.241CysLys: 0.241 ± 0.12
0.782CysLeu: 0.782 ± 0.222
0.18CysMet: 0.18 ± 0.128
0.541CysAsn: 0.541 ± 0.162
0.481CysPro: 0.481 ± 0.192
0.241CysGln: 0.241 ± 0.133
0.361CysArg: 0.361 ± 0.129
0.541CysSer: 0.541 ± 0.189
0.601CysThr: 0.601 ± 0.171
0.601CysVal: 0.601 ± 0.179
0.301CysTrp: 0.301 ± 0.131
0.361CysTyr: 0.361 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
6.434AspAla: 6.434 ± 0.568
0.722AspCys: 0.722 ± 0.204
3.067AspAsp: 3.067 ± 0.496
4.029AspGlu: 4.029 ± 0.566
2.465AspPhe: 2.465 ± 0.435
5.652AspGly: 5.652 ± 0.542
1.383AspHis: 1.383 ± 0.264
3.367AspIle: 3.367 ± 0.393
2.706AspLys: 2.706 ± 0.391
5.652AspLeu: 5.652 ± 0.662
1.443AspMet: 1.443 ± 0.313
1.984AspAsn: 1.984 ± 0.326
3.969AspPro: 3.969 ± 0.58
2.225AspGln: 2.225 ± 0.307
2.946AspArg: 2.946 ± 0.479
3.608AspSer: 3.608 ± 0.391
3.367AspThr: 3.367 ± 0.379
3.969AspVal: 3.969 ± 0.468
1.203AspTrp: 1.203 ± 0.345
2.526AspTyr: 2.526 ± 0.37
0.0AspXaa: 0.0 ± 0.0
Glu
6.615GluAla: 6.615 ± 0.737
0.12GluCys: 0.12 ± 0.1
3.909GluAsp: 3.909 ± 0.608
4.51GluGlu: 4.51 ± 0.624
2.946GluPhe: 2.946 ± 0.461
5.171GluGly: 5.171 ± 0.585
1.383GluHis: 1.383 ± 0.259
3.848GluIle: 3.848 ± 0.496
2.465GluLys: 2.465 ± 0.431
5.893GluLeu: 5.893 ± 0.722
2.285GluMet: 2.285 ± 0.34
2.165GluAsn: 2.165 ± 0.328
2.646GluPro: 2.646 ± 0.428
2.044GluGln: 2.044 ± 0.347
4.089GluArg: 4.089 ± 0.602
3.247GluSer: 3.247 ± 0.375
3.428GluThr: 3.428 ± 0.465
4.33GluVal: 4.33 ± 0.414
1.563GluTrp: 1.563 ± 0.338
1.924GluTyr: 1.924 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
3.127PheAla: 3.127 ± 0.424
0.241PheCys: 0.241 ± 0.113
2.646PheAsp: 2.646 ± 0.555
2.105PheGlu: 2.105 ± 0.404
0.722PhePhe: 0.722 ± 0.212
3.548PheGly: 3.548 ± 0.49
0.601PheHis: 0.601 ± 0.175
1.744PheIle: 1.744 ± 0.33
1.263PheLys: 1.263 ± 0.187
2.405PheLeu: 2.405 ± 0.367
0.661PheMet: 0.661 ± 0.223
1.924PheAsn: 1.924 ± 0.373
1.563PhePro: 1.563 ± 0.257
0.962PheGln: 0.962 ± 0.226
1.684PheArg: 1.684 ± 0.303
2.225PheSer: 2.225 ± 0.347
2.586PheThr: 2.586 ± 0.381
2.044PheVal: 2.044 ± 0.374
0.421PheTrp: 0.421 ± 0.169
0.722PheTyr: 0.722 ± 0.234
0.0PheXaa: 0.0 ± 0.0
Gly
7.637GlyAla: 7.637 ± 0.916
0.962GlyCys: 0.962 ± 0.255
6.073GlyAsp: 6.073 ± 0.621
4.51GlyGlu: 4.51 ± 0.508
3.127GlyPhe: 3.127 ± 0.417
9.2GlyGly: 9.2 ± 1.476
1.624GlyHis: 1.624 ± 0.312
3.969GlyIle: 3.969 ± 0.564
5.051GlyLys: 5.051 ± 0.711
7.396GlyLeu: 7.396 ± 0.985
2.706GlyMet: 2.706 ± 0.478
3.367GlyAsn: 3.367 ± 0.498
3.127GlyPro: 3.127 ± 0.413
2.826GlyGln: 2.826 ± 0.527
3.909GlyArg: 3.909 ± 0.416
4.57GlySer: 4.57 ± 0.73
6.554GlyThr: 6.554 ± 0.731
5.532GlyVal: 5.532 ± 0.523
1.563GlyTrp: 1.563 ± 0.265
2.465GlyTyr: 2.465 ± 0.379
0.0GlyXaa: 0.0 ± 0.0
His
1.563HisAla: 1.563 ± 0.263
0.301HisCys: 0.301 ± 0.133
1.383HisAsp: 1.383 ± 0.313
0.962HisGlu: 0.962 ± 0.223
0.782HisPhe: 0.782 ± 0.219
1.563HisGly: 1.563 ± 0.393
0.722HisHis: 0.722 ± 0.191
0.842HisIle: 0.842 ± 0.2
0.902HisLys: 0.902 ± 0.263
1.022HisLeu: 1.022 ± 0.257
0.481HisMet: 0.481 ± 0.174
0.481HisAsn: 0.481 ± 0.176
1.022HisPro: 1.022 ± 0.219
0.842HisGln: 0.842 ± 0.248
1.263HisArg: 1.263 ± 0.295
0.842HisSer: 0.842 ± 0.221
1.263HisThr: 1.263 ± 0.27
1.143HisVal: 1.143 ± 0.285
0.601HisTrp: 0.601 ± 0.182
0.661HisTyr: 0.661 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
4.991IleAla: 4.991 ± 0.569
0.421IleCys: 0.421 ± 0.142
3.788IleAsp: 3.788 ± 0.415
3.608IleGlu: 3.608 ± 0.535
1.443IlePhe: 1.443 ± 0.318
4.209IleGly: 4.209 ± 0.687
0.722IleHis: 0.722 ± 0.207
1.503IleIle: 1.503 ± 0.247
2.345IleLys: 2.345 ± 0.356
3.608IleLeu: 3.608 ± 0.378
0.722IleMet: 0.722 ± 0.226
2.225IleAsn: 2.225 ± 0.38
3.728IlePro: 3.728 ± 0.475
1.263IleGln: 1.263 ± 0.246
3.788IleArg: 3.788 ± 0.539
3.007IleSer: 3.007 ± 0.481
3.728IleThr: 3.728 ± 0.418
3.187IleVal: 3.187 ± 0.47
0.722IleTrp: 0.722 ± 0.207
1.143IleTyr: 1.143 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
5.592LysAla: 5.592 ± 0.575
0.301LysCys: 0.301 ± 0.135
2.044LysAsp: 2.044 ± 0.322
3.548LysGlu: 3.548 ± 0.482
1.082LysPhe: 1.082 ± 0.216
3.788LysGly: 3.788 ± 0.429
0.541LysHis: 0.541 ± 0.184
2.465LysIle: 2.465 ± 0.428
2.826LysLys: 2.826 ± 0.484
4.149LysLeu: 4.149 ± 0.406
1.323LysMet: 1.323 ± 0.321
1.082LysAsn: 1.082 ± 0.24
2.826LysPro: 2.826 ± 0.558
1.684LysGln: 1.684 ± 0.512
3.488LysArg: 3.488 ± 0.634
2.706LysSer: 2.706 ± 0.362
2.946LysThr: 2.946 ± 0.426
3.668LysVal: 3.668 ± 0.665
0.782LysTrp: 0.782 ± 0.222
1.503LysTyr: 1.503 ± 0.325
0.0LysXaa: 0.0 ± 0.0
Leu
9.621LeuAla: 9.621 ± 0.931
0.842LeuCys: 0.842 ± 0.284
5.472LeuAsp: 5.472 ± 0.601
5.232LeuGlu: 5.232 ± 0.628
2.526LeuPhe: 2.526 ± 0.43
7.637LeuGly: 7.637 ± 1.112
1.263LeuHis: 1.263 ± 0.342
4.33LeuIle: 4.33 ± 0.478
3.728LeuLys: 3.728 ± 0.476
5.833LeuLeu: 5.833 ± 0.771
2.044LeuMet: 2.044 ± 0.404
2.646LeuAsn: 2.646 ± 0.344
4.51LeuPro: 4.51 ± 0.511
2.766LeuGln: 2.766 ± 0.578
4.75LeuArg: 4.75 ± 0.526
4.991LeuSer: 4.991 ± 0.487
5.713LeuThr: 5.713 ± 0.699
4.69LeuVal: 4.69 ± 0.454
1.383LeuTrp: 1.383 ± 0.234
1.924LeuTyr: 1.924 ± 0.392
0.0LeuXaa: 0.0 ± 0.0
Met
3.307MetAla: 3.307 ± 0.428
0.301MetCys: 0.301 ± 0.135
0.782MetAsp: 0.782 ± 0.217
1.624MetGlu: 1.624 ± 0.325
0.481MetPhe: 0.481 ± 0.17
2.105MetGly: 2.105 ± 0.324
0.481MetHis: 0.481 ± 0.165
1.563MetIle: 1.563 ± 0.307
1.263MetLys: 1.263 ± 0.283
1.143MetLeu: 1.143 ± 0.288
0.481MetMet: 0.481 ± 0.19
0.782MetAsn: 0.782 ± 0.197
1.143MetPro: 1.143 ± 0.31
1.022MetGln: 1.022 ± 0.232
2.044MetArg: 2.044 ± 0.377
2.586MetSer: 2.586 ± 0.359
2.044MetThr: 2.044 ± 0.339
1.443MetVal: 1.443 ± 0.245
0.541MetTrp: 0.541 ± 0.181
0.661MetTyr: 0.661 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
3.969AsnAla: 3.969 ± 0.486
0.241AsnCys: 0.241 ± 0.114
1.744AsnAsp: 1.744 ± 0.327
2.285AsnGlu: 2.285 ± 0.415
1.082AsnPhe: 1.082 ± 0.363
3.608AsnGly: 3.608 ± 0.479
0.962AsnHis: 0.962 ± 0.246
1.563AsnIle: 1.563 ± 0.328
0.782AsnLys: 0.782 ± 0.225
3.187AsnLeu: 3.187 ± 0.48
0.782AsnMet: 0.782 ± 0.232
0.722AsnAsn: 0.722 ± 0.202
2.706AsnPro: 2.706 ± 0.314
1.022AsnGln: 1.022 ± 0.231
1.984AsnArg: 1.984 ± 0.389
2.044AsnSer: 2.044 ± 0.354
1.503AsnThr: 1.503 ± 0.243
2.586AsnVal: 2.586 ± 0.401
0.722AsnTrp: 0.722 ± 0.213
1.082AsnTyr: 1.082 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
4.45ProAla: 4.45 ± 0.579
0.421ProCys: 0.421 ± 0.172
3.428ProAsp: 3.428 ± 0.526
4.57ProGlu: 4.57 ± 0.622
1.624ProPhe: 1.624 ± 0.308
4.209ProGly: 4.209 ± 0.513
1.022ProHis: 1.022 ± 0.309
2.405ProIle: 2.405 ± 0.316
2.586ProLys: 2.586 ± 0.495
3.668ProLeu: 3.668 ± 0.491
1.143ProMet: 1.143 ± 0.265
2.165ProAsn: 2.165 ± 0.355
2.044ProPro: 2.044 ± 0.308
1.443ProGln: 1.443 ± 0.315
2.886ProArg: 2.886 ± 0.512
2.946ProSer: 2.946 ± 0.397
4.089ProThr: 4.089 ± 0.584
3.548ProVal: 3.548 ± 0.558
0.481ProTrp: 0.481 ± 0.181
1.323ProTyr: 1.323 ± 0.203
0.0ProXaa: 0.0 ± 0.0
Gln
4.51GlnAla: 4.51 ± 0.823
0.06GlnCys: 0.06 ± 0.072
1.443GlnAsp: 1.443 ± 0.276
1.684GlnGlu: 1.684 ± 0.253
1.203GlnPhe: 1.203 ± 0.244
2.706GlnGly: 2.706 ± 0.449
0.722GlnHis: 0.722 ± 0.184
2.345GlnIle: 2.345 ± 0.368
1.744GlnLys: 1.744 ± 0.373
2.646GlnLeu: 2.646 ± 0.722
1.203GlnMet: 1.203 ± 0.283
0.962GlnAsn: 0.962 ± 0.226
1.143GlnPro: 1.143 ± 0.296
1.503GlnGln: 1.503 ± 0.363
2.165GlnArg: 2.165 ± 0.367
1.864GlnSer: 1.864 ± 0.306
1.984GlnThr: 1.984 ± 0.391
2.646GlnVal: 2.646 ± 0.341
0.902GlnTrp: 0.902 ± 0.22
1.203GlnTyr: 1.203 ± 0.297
0.0GlnXaa: 0.0 ± 0.0
Arg
5.292ArgAla: 5.292 ± 0.574
0.782ArgCys: 0.782 ± 0.261
4.269ArgAsp: 4.269 ± 0.609
3.668ArgGlu: 3.668 ± 0.498
2.225ArgPhe: 2.225 ± 0.444
4.029ArgGly: 4.029 ± 0.493
1.203ArgHis: 1.203 ± 0.299
3.728ArgIle: 3.728 ± 0.45
2.826ArgLys: 2.826 ± 0.456
5.352ArgLeu: 5.352 ± 0.653
1.383ArgMet: 1.383 ± 0.348
2.285ArgAsn: 2.285 ± 0.359
2.706ArgPro: 2.706 ± 0.413
2.225ArgGln: 2.225 ± 0.323
4.69ArgArg: 4.69 ± 0.652
3.067ArgSer: 3.067 ± 0.405
3.127ArgThr: 3.127 ± 0.401
3.909ArgVal: 3.909 ± 0.402
1.203ArgTrp: 1.203 ± 0.25
1.864ArgTyr: 1.864 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
5.051SerAla: 5.051 ± 0.638
0.421SerCys: 0.421 ± 0.15
3.668SerAsp: 3.668 ± 0.415
3.848SerGlu: 3.848 ± 0.483
1.924SerPhe: 1.924 ± 0.379
5.713SerGly: 5.713 ± 0.941
0.782SerHis: 0.782 ± 0.196
2.646SerIle: 2.646 ± 0.408
2.946SerLys: 2.946 ± 0.456
4.811SerLeu: 4.811 ± 0.595
1.624SerMet: 1.624 ± 0.286
1.503SerAsn: 1.503 ± 0.278
2.766SerPro: 2.766 ± 0.426
1.864SerGln: 1.864 ± 0.4
3.848SerArg: 3.848 ± 0.544
3.247SerSer: 3.247 ± 0.518
2.706SerThr: 2.706 ± 0.46
4.45SerVal: 4.45 ± 0.41
1.203SerTrp: 1.203 ± 0.244
1.684SerTyr: 1.684 ± 0.245
0.0SerXaa: 0.0 ± 0.0
Thr
7.035ThrAla: 7.035 ± 0.618
0.421ThrCys: 0.421 ± 0.141
3.909ThrAsp: 3.909 ± 0.53
3.007ThrGlu: 3.007 ± 0.405
2.105ThrPhe: 2.105 ± 0.362
6.013ThrGly: 6.013 ± 0.779
1.263ThrHis: 1.263 ± 0.238
2.826ThrIle: 2.826 ± 0.394
2.826ThrLys: 2.826 ± 0.486
6.013ThrLeu: 6.013 ± 0.561
1.804ThrMet: 1.804 ± 0.273
1.624ThrAsn: 1.624 ± 0.336
3.909ThrPro: 3.909 ± 0.393
2.225ThrGln: 2.225 ± 0.36
3.367ThrArg: 3.367 ± 0.463
3.367ThrSer: 3.367 ± 0.531
3.909ThrThr: 3.909 ± 0.524
4.931ThrVal: 4.931 ± 0.776
1.082ThrTrp: 1.082 ± 0.368
2.646ThrTyr: 2.646 ± 0.398
0.0ThrXaa: 0.0 ± 0.0
Val
5.532ValAla: 5.532 ± 0.695
0.661ValCys: 0.661 ± 0.17
5.412ValAsp: 5.412 ± 0.572
4.75ValGlu: 4.75 ± 0.57
2.766ValPhe: 2.766 ± 0.56
4.931ValGly: 4.931 ± 0.545
1.082ValHis: 1.082 ± 0.228
3.548ValIle: 3.548 ± 0.492
4.149ValLys: 4.149 ± 0.448
4.931ValLeu: 4.931 ± 0.666
1.143ValMet: 1.143 ± 0.268
2.886ValAsn: 2.886 ± 0.459
3.187ValPro: 3.187 ± 0.4
1.924ValGln: 1.924 ± 0.355
3.608ValArg: 3.608 ± 0.509
4.33ValSer: 4.33 ± 0.455
4.991ValThr: 4.991 ± 0.416
5.352ValVal: 5.352 ± 0.556
1.503ValTrp: 1.503 ± 0.322
1.744ValTyr: 1.744 ± 0.343
0.0ValXaa: 0.0 ± 0.0
Trp
1.864TrpAla: 1.864 ± 0.401
0.481TrpCys: 0.481 ± 0.175
1.624TrpAsp: 1.624 ± 0.31
0.842TrpGlu: 0.842 ± 0.197
0.601TrpPhe: 0.601 ± 0.17
1.503TrpGly: 1.503 ± 0.388
0.361TrpHis: 0.361 ± 0.157
0.782TrpIle: 0.782 ± 0.195
0.722TrpLys: 0.722 ± 0.19
1.203TrpLeu: 1.203 ± 0.247
0.902TrpMet: 0.902 ± 0.259
0.722TrpAsn: 0.722 ± 0.226
0.962TrpPro: 0.962 ± 0.243
1.383TrpGln: 1.383 ± 0.307
0.782TrpArg: 0.782 ± 0.258
0.782TrpSer: 0.782 ± 0.236
1.263TrpThr: 1.263 ± 0.25
1.263TrpVal: 1.263 ± 0.245
0.301TrpTrp: 0.301 ± 0.158
0.481TrpTyr: 0.481 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.187TyrAla: 3.187 ± 0.425
0.301TyrCys: 0.301 ± 0.188
2.044TyrAsp: 2.044 ± 0.361
2.105TyrGlu: 2.105 ± 0.365
0.722TyrPhe: 0.722 ± 0.17
1.924TyrGly: 1.924 ± 0.318
0.421TyrHis: 0.421 ± 0.172
1.443TyrIle: 1.443 ± 0.305
1.022TyrLys: 1.022 ± 0.256
3.307TyrLeu: 3.307 ± 0.486
0.301TyrMet: 0.301 ± 0.141
0.902TyrAsn: 0.902 ± 0.241
1.443TyrPro: 1.443 ± 0.305
1.443TyrGln: 1.443 ± 0.329
1.924TyrArg: 1.924 ± 0.35
1.203TyrSer: 1.203 ± 0.267
2.105TyrThr: 2.105 ± 0.37
2.225TyrVal: 2.225 ± 0.36
0.722TyrTrp: 0.722 ± 0.231
0.902TyrTyr: 0.902 ± 0.249
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (16631 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski