Amino acid dipepetide frequency for Prochlorococcus phage P-GSP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.908AlaAla: 8.908 ± 1.049
0.771AlaCys: 0.771 ± 0.358
6.338AlaAsp: 6.338 ± 1.279
4.711AlaGlu: 4.711 ± 0.747
3.169AlaPhe: 3.169 ± 0.674
6.595AlaGly: 6.595 ± 0.777
0.942AlaHis: 0.942 ± 0.319
5.739AlaIle: 5.739 ± 0.701
8.308AlaLys: 8.308 ± 1.613
6.767AlaLeu: 6.767 ± 0.695
2.056AlaMet: 2.056 ± 0.595
6.681AlaAsn: 6.681 ± 1.07
3.512AlaPro: 3.512 ± 0.539
2.827AlaGln: 2.827 ± 0.503
4.368AlaArg: 4.368 ± 0.858
5.739AlaSer: 5.739 ± 0.716
6.081AlaThr: 6.081 ± 0.757
5.054AlaVal: 5.054 ± 0.947
0.685AlaTrp: 0.685 ± 0.226
2.57AlaTyr: 2.57 ± 0.465
0.0AlaXaa: 0.0 ± 0.0
Cys
0.6CysAla: 0.6 ± 0.241
0.0CysCys: 0.0 ± 0.0
0.6CysAsp: 0.6 ± 0.215
0.428CysGlu: 0.428 ± 0.172
0.086CysPhe: 0.086 ± 0.084
0.514CysGly: 0.514 ± 0.189
0.086CysHis: 0.086 ± 0.083
0.257CysIle: 0.257 ± 0.145
0.343CysLys: 0.343 ± 0.157
0.514CysLeu: 0.514 ± 0.251
0.171CysMet: 0.171 ± 0.111
0.428CysAsn: 0.428 ± 0.176
0.428CysPro: 0.428 ± 0.262
0.171CysGln: 0.171 ± 0.112
0.514CysArg: 0.514 ± 0.168
0.6CysSer: 0.6 ± 0.331
0.685CysThr: 0.685 ± 0.264
0.685CysVal: 0.685 ± 0.312
0.0CysTrp: 0.0 ± 0.0
0.171CysTyr: 0.171 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
6.081AspAla: 6.081 ± 0.932
0.685AspCys: 0.685 ± 0.231
4.625AspAsp: 4.625 ± 0.671
3.512AspGlu: 3.512 ± 0.569
2.141AspPhe: 2.141 ± 0.354
5.739AspGly: 5.739 ± 0.954
0.942AspHis: 0.942 ± 0.359
3.683AspIle: 3.683 ± 0.489
3.94AspLys: 3.94 ± 0.679
6.51AspLeu: 6.51 ± 0.794
0.771AspMet: 0.771 ± 0.24
3.255AspAsn: 3.255 ± 0.435
1.713AspPro: 1.713 ± 0.34
1.37AspGln: 1.37 ± 0.241
2.313AspArg: 2.313 ± 0.547
3.597AspSer: 3.597 ± 0.889
4.283AspThr: 4.283 ± 0.585
3.512AspVal: 3.512 ± 0.541
0.428AspTrp: 0.428 ± 0.188
1.884AspTyr: 1.884 ± 0.426
0.0AspXaa: 0.0 ± 0.0
Glu
5.824GluAla: 5.824 ± 1.021
0.086GluCys: 0.086 ± 0.079
3.34GluAsp: 3.34 ± 0.578
4.54GluGlu: 4.54 ± 1.048
1.97GluPhe: 1.97 ± 0.419
4.368GluGly: 4.368 ± 0.446
1.199GluHis: 1.199 ± 0.329
3.426GluIle: 3.426 ± 0.616
2.998GluLys: 2.998 ± 0.682
5.739GluLeu: 5.739 ± 0.646
1.199GluMet: 1.199 ± 0.344
1.884GluAsn: 1.884 ± 0.459
1.884GluPro: 1.884 ± 0.379
3.597GluGln: 3.597 ± 0.661
3.084GluArg: 3.084 ± 0.597
2.57GluSer: 2.57 ± 0.316
3.512GluThr: 3.512 ± 0.502
3.169GluVal: 3.169 ± 0.552
1.113GluTrp: 1.113 ± 0.361
1.627GluTyr: 1.627 ± 0.378
0.0GluXaa: 0.0 ± 0.0
Phe
2.398PheAla: 2.398 ± 0.422
0.428PheCys: 0.428 ± 0.196
1.799PheAsp: 1.799 ± 0.43
2.056PheGlu: 2.056 ± 0.533
1.456PhePhe: 1.456 ± 0.407
3.34PheGly: 3.34 ± 0.508
0.514PheHis: 0.514 ± 0.199
1.37PheIle: 1.37 ± 0.25
1.542PheLys: 1.542 ± 0.397
2.998PheLeu: 2.998 ± 0.518
1.113PheMet: 1.113 ± 0.304
2.827PheAsn: 2.827 ± 0.716
1.542PhePro: 1.542 ± 0.401
1.627PheGln: 1.627 ± 0.422
1.799PheArg: 1.799 ± 0.441
2.141PheSer: 2.141 ± 0.437
2.313PheThr: 2.313 ± 0.433
2.827PheVal: 2.827 ± 0.424
0.428PheTrp: 0.428 ± 0.216
0.771PheTyr: 0.771 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
5.91GlyAla: 5.91 ± 0.93
0.171GlyCys: 0.171 ± 0.121
4.711GlyAsp: 4.711 ± 0.686
3.426GlyGlu: 3.426 ± 0.556
2.827GlyPhe: 2.827 ± 0.5
4.968GlyGly: 4.968 ± 0.854
0.942GlyHis: 0.942 ± 0.327
4.454GlyIle: 4.454 ± 0.488
4.026GlyLys: 4.026 ± 0.62
5.482GlyLeu: 5.482 ± 0.678
1.97GlyMet: 1.97 ± 0.557
4.454GlyAsn: 4.454 ± 0.681
1.627GlyPro: 1.627 ± 0.492
2.912GlyGln: 2.912 ± 0.504
3.084GlyArg: 3.084 ± 0.548
6.253GlySer: 6.253 ± 1.373
5.653GlyThr: 5.653 ± 1.018
4.197GlyVal: 4.197 ± 0.631
0.942GlyTrp: 0.942 ± 0.243
2.827GlyTyr: 2.827 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
0.771HisAla: 0.771 ± 0.233
0.171HisCys: 0.171 ± 0.121
1.028HisAsp: 1.028 ± 0.237
0.942HisGlu: 0.942 ± 0.283
0.857HisPhe: 0.857 ± 0.307
1.37HisGly: 1.37 ± 0.284
0.428HisHis: 0.428 ± 0.191
1.285HisIle: 1.285 ± 0.333
0.942HisLys: 0.942 ± 0.297
1.028HisLeu: 1.028 ± 0.308
0.171HisMet: 0.171 ± 0.12
1.285HisAsn: 1.285 ± 0.321
0.6HisPro: 0.6 ± 0.2
0.685HisGln: 0.685 ± 0.2
0.514HisArg: 0.514 ± 0.204
0.6HisSer: 0.6 ± 0.205
1.37HisThr: 1.37 ± 0.39
0.6HisVal: 0.6 ± 0.24
0.257HisTrp: 0.257 ± 0.143
0.942HisTyr: 0.942 ± 0.308
0.0HisXaa: 0.0 ± 0.0
Ile
6.852IleAla: 6.852 ± 1.245
0.343IleCys: 0.343 ± 0.196
3.512IleAsp: 3.512 ± 0.536
2.398IleGlu: 2.398 ± 0.49
1.97IlePhe: 1.97 ± 0.339
3.084IleGly: 3.084 ± 0.694
1.713IleHis: 1.713 ± 0.335
2.57IleIle: 2.57 ± 0.42
4.197IleLys: 4.197 ± 0.632
3.512IleLeu: 3.512 ± 0.491
0.685IleMet: 0.685 ± 0.295
2.912IleAsn: 2.912 ± 0.418
2.398IlePro: 2.398 ± 0.623
3.426IleGln: 3.426 ± 0.46
2.827IleArg: 2.827 ± 0.543
4.197IleSer: 4.197 ± 0.475
5.054IleThr: 5.054 ± 0.985
2.912IleVal: 2.912 ± 0.509
0.685IleTrp: 0.685 ± 0.275
1.799IleTyr: 1.799 ± 0.367
0.0IleXaa: 0.0 ± 0.0
Lys
6.167LysAla: 6.167 ± 1.195
0.685LysCys: 0.685 ± 0.24
3.084LysAsp: 3.084 ± 0.509
3.683LysGlu: 3.683 ± 0.694
2.398LysPhe: 2.398 ± 0.458
2.912LysGly: 2.912 ± 0.736
1.199LysHis: 1.199 ± 0.317
5.396LysIle: 5.396 ± 1.367
5.225LysLys: 5.225 ± 2.036
7.281LysLeu: 7.281 ± 0.783
1.884LysMet: 1.884 ± 0.391
2.998LysAsn: 2.998 ± 0.597
2.998LysPro: 2.998 ± 0.551
2.056LysGln: 2.056 ± 0.497
3.169LysArg: 3.169 ± 0.637
4.026LysSer: 4.026 ± 0.823
3.94LysThr: 3.94 ± 0.803
3.255LysVal: 3.255 ± 0.707
0.428LysTrp: 0.428 ± 0.196
2.313LysTyr: 2.313 ± 0.491
0.0LysXaa: 0.0 ± 0.0
Leu
6.938LeuAla: 6.938 ± 0.835
0.771LeuCys: 0.771 ± 0.333
5.225LeuAsp: 5.225 ± 0.57
4.197LeuGlu: 4.197 ± 0.64
2.57LeuPhe: 2.57 ± 0.388
4.968LeuGly: 4.968 ± 0.609
1.542LeuHis: 1.542 ± 0.329
4.197LeuIle: 4.197 ± 0.682
6.167LeuLys: 6.167 ± 1.108
4.54LeuLeu: 4.54 ± 0.739
1.799LeuMet: 1.799 ± 0.428
3.683LeuAsn: 3.683 ± 0.493
3.512LeuPro: 3.512 ± 0.763
4.026LeuGln: 4.026 ± 0.92
4.197LeuArg: 4.197 ± 0.716
5.482LeuSer: 5.482 ± 0.66
4.711LeuThr: 4.711 ± 0.591
5.139LeuVal: 5.139 ± 0.735
0.685LeuTrp: 0.685 ± 0.253
3.084LeuTyr: 3.084 ± 0.51
0.0LeuXaa: 0.0 ± 0.0
Met
2.57MetAla: 2.57 ± 0.729
0.171MetCys: 0.171 ± 0.134
1.113MetAsp: 1.113 ± 0.316
1.028MetGlu: 1.028 ± 0.309
0.771MetPhe: 0.771 ± 0.374
1.713MetGly: 1.713 ± 0.508
0.257MetHis: 0.257 ± 0.253
1.199MetIle: 1.199 ± 0.322
1.285MetLys: 1.285 ± 0.389
1.884MetLeu: 1.884 ± 0.515
0.685MetMet: 0.685 ± 0.313
1.199MetAsn: 1.199 ± 0.347
0.857MetPro: 0.857 ± 0.297
1.199MetGln: 1.199 ± 0.28
1.627MetArg: 1.627 ± 0.448
1.627MetSer: 1.627 ± 0.348
1.713MetThr: 1.713 ± 0.369
1.199MetVal: 1.199 ± 0.252
0.171MetTrp: 0.171 ± 0.108
0.685MetTyr: 0.685 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
5.054AsnAla: 5.054 ± 0.678
0.257AsnCys: 0.257 ± 0.194
2.056AsnAsp: 2.056 ± 0.422
2.227AsnGlu: 2.227 ± 0.384
2.912AsnPhe: 2.912 ± 0.588
5.225AsnGly: 5.225 ± 0.823
0.942AsnHis: 0.942 ± 0.241
2.398AsnIle: 2.398 ± 0.463
2.912AsnLys: 2.912 ± 0.609
3.854AsnLeu: 3.854 ± 0.509
0.685AsnMet: 0.685 ± 0.243
5.139AsnAsn: 5.139 ± 1.029
3.084AsnPro: 3.084 ± 0.497
2.741AsnGln: 2.741 ± 0.348
3.34AsnArg: 3.34 ± 0.553
5.91AsnSer: 5.91 ± 0.884
4.368AsnThr: 4.368 ± 0.838
3.769AsnVal: 3.769 ± 0.483
0.771AsnTrp: 0.771 ± 0.261
1.713AsnTyr: 1.713 ± 0.362
0.0AsnXaa: 0.0 ± 0.0
Pro
2.912ProAla: 2.912 ± 0.531
0.086ProCys: 0.086 ± 0.083
2.056ProAsp: 2.056 ± 0.34
3.34ProGlu: 3.34 ± 0.766
1.456ProPhe: 1.456 ± 0.397
2.056ProGly: 2.056 ± 0.452
0.514ProHis: 0.514 ± 0.17
2.056ProIle: 2.056 ± 0.394
2.57ProLys: 2.57 ± 0.619
2.313ProLeu: 2.313 ± 0.527
0.771ProMet: 0.771 ± 0.265
1.456ProAsn: 1.456 ± 0.33
2.313ProPro: 2.313 ± 1.112
2.056ProGln: 2.056 ± 0.453
0.942ProArg: 0.942 ± 0.282
2.827ProSer: 2.827 ± 0.479
3.426ProThr: 3.426 ± 0.468
2.741ProVal: 2.741 ± 0.583
0.857ProTrp: 0.857 ± 0.285
1.542ProTyr: 1.542 ± 0.412
0.0ProXaa: 0.0 ± 0.0
Gln
4.283GlnAla: 4.283 ± 0.785
0.428GlnCys: 0.428 ± 0.189
1.799GlnAsp: 1.799 ± 0.402
2.741GlnGlu: 2.741 ± 0.721
1.285GlnPhe: 1.285 ± 0.36
2.912GlnGly: 2.912 ± 0.52
0.771GlnHis: 0.771 ± 0.244
2.827GlnIle: 2.827 ± 0.606
2.655GlnLys: 2.655 ± 0.564
3.854GlnLeu: 3.854 ± 0.586
1.37GlnMet: 1.37 ± 0.364
2.57GlnAsn: 2.57 ± 0.462
1.113GlnPro: 1.113 ± 0.307
2.227GlnGln: 2.227 ± 0.437
1.627GlnArg: 1.627 ± 0.343
2.398GlnSer: 2.398 ± 0.338
3.426GlnThr: 3.426 ± 0.419
3.169GlnVal: 3.169 ± 0.478
0.514GlnTrp: 0.514 ± 0.188
1.542GlnTyr: 1.542 ± 0.427
0.0GlnXaa: 0.0 ± 0.0
Arg
4.968ArgAla: 4.968 ± 0.704
0.171ArgCys: 0.171 ± 0.112
2.484ArgAsp: 2.484 ± 0.432
3.94ArgGlu: 3.94 ± 0.625
1.799ArgPhe: 1.799 ± 0.31
2.57ArgGly: 2.57 ± 0.611
0.343ArgHis: 0.343 ± 0.161
2.141ArgIle: 2.141 ± 0.438
3.084ArgLys: 3.084 ± 0.561
3.683ArgLeu: 3.683 ± 0.598
1.884ArgMet: 1.884 ± 0.309
2.313ArgAsn: 2.313 ± 0.401
1.285ArgPro: 1.285 ± 0.326
1.799ArgGln: 1.799 ± 0.435
1.542ArgArg: 1.542 ± 0.495
2.912ArgSer: 2.912 ± 0.439
2.57ArgThr: 2.57 ± 0.554
2.57ArgVal: 2.57 ± 0.469
0.771ArgTrp: 0.771 ± 0.279
1.97ArgTyr: 1.97 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
5.054SerAla: 5.054 ± 1.055
0.6SerCys: 0.6 ± 0.264
4.797SerAsp: 4.797 ± 0.559
3.512SerGlu: 3.512 ± 0.556
1.97SerPhe: 1.97 ± 0.378
6.595SerGly: 6.595 ± 1.269
0.942SerHis: 0.942 ± 0.327
3.769SerIle: 3.769 ± 0.814
3.854SerLys: 3.854 ± 0.638
4.54SerLeu: 4.54 ± 0.649
1.884SerMet: 1.884 ± 0.393
5.396SerAsn: 5.396 ± 1.065
2.655SerPro: 2.655 ± 0.476
3.512SerGln: 3.512 ± 0.494
2.912SerArg: 2.912 ± 0.729
5.482SerSer: 5.482 ± 1.222
5.054SerThr: 5.054 ± 0.715
4.711SerVal: 4.711 ± 0.768
0.771SerTrp: 0.771 ± 0.255
2.141SerTyr: 2.141 ± 0.388
0.0SerXaa: 0.0 ± 0.0
Thr
7.794ThrAla: 7.794 ± 1.583
0.514ThrCys: 0.514 ± 0.188
4.111ThrAsp: 4.111 ± 0.578
3.769ThrGlu: 3.769 ± 0.453
2.57ThrPhe: 2.57 ± 0.45
5.653ThrGly: 5.653 ± 0.95
0.942ThrHis: 0.942 ± 0.369
4.711ThrIle: 4.711 ± 0.816
4.197ThrLys: 4.197 ± 0.985
5.824ThrLeu: 5.824 ± 0.648
1.542ThrMet: 1.542 ± 0.375
4.368ThrAsn: 4.368 ± 0.751
3.597ThrPro: 3.597 ± 0.771
1.713ThrGln: 1.713 ± 0.322
2.398ThrArg: 2.398 ± 0.455
6.253ThrSer: 6.253 ± 1.15
8.223ThrThr: 8.223 ± 1.867
3.34ThrVal: 3.34 ± 0.648
1.113ThrTrp: 1.113 ± 0.296
3.255ThrTyr: 3.255 ± 0.498
0.0ThrXaa: 0.0 ± 0.0
Val
4.197ValAla: 4.197 ± 0.682
0.514ValCys: 0.514 ± 0.203
4.882ValAsp: 4.882 ± 0.811
3.34ValGlu: 3.34 ± 0.544
1.97ValPhe: 1.97 ± 0.513
3.597ValGly: 3.597 ± 0.534
0.771ValHis: 0.771 ± 0.238
3.255ValIle: 3.255 ± 0.46
3.512ValLys: 3.512 ± 0.57
3.34ValLeu: 3.34 ± 0.597
1.37ValMet: 1.37 ± 0.305
3.769ValAsn: 3.769 ± 0.535
1.713ValPro: 1.713 ± 0.472
3.34ValGln: 3.34 ± 0.409
2.056ValArg: 2.056 ± 0.347
4.625ValSer: 4.625 ± 0.738
6.424ValThr: 6.424 ± 1.397
4.454ValVal: 4.454 ± 0.771
0.428ValTrp: 0.428 ± 0.147
2.313ValTyr: 2.313 ± 0.478
0.0ValXaa: 0.0 ± 0.0
Trp
1.37TrpAla: 1.37 ± 0.452
0.257TrpCys: 0.257 ± 0.176
0.6TrpAsp: 0.6 ± 0.235
1.028TrpGlu: 1.028 ± 0.349
0.257TrpPhe: 0.257 ± 0.18
0.428TrpGly: 0.428 ± 0.167
0.086TrpHis: 0.086 ± 0.099
0.428TrpIle: 0.428 ± 0.186
1.113TrpLys: 1.113 ± 0.316
1.285TrpLeu: 1.285 ± 0.306
0.257TrpMet: 0.257 ± 0.129
0.6TrpAsn: 0.6 ± 0.256
0.0TrpPro: 0.0 ± 0.0
0.428TrpGln: 0.428 ± 0.147
0.428TrpArg: 0.428 ± 0.168
0.942TrpSer: 0.942 ± 0.216
0.857TrpThr: 0.857 ± 0.413
0.6TrpVal: 0.6 ± 0.222
0.086TrpTrp: 0.086 ± 0.083
0.343TrpTyr: 0.343 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.169TyrAla: 3.169 ± 0.481
0.171TyrCys: 0.171 ± 0.111
2.827TyrAsp: 2.827 ± 0.516
2.313TyrGlu: 2.313 ± 0.567
0.942TyrPhe: 0.942 ± 0.292
2.227TyrGly: 2.227 ± 0.349
0.771TyrHis: 0.771 ± 0.222
1.884TyrIle: 1.884 ± 0.301
2.227TyrLys: 2.227 ± 0.401
2.484TyrLeu: 2.484 ± 0.577
0.6TyrMet: 0.6 ± 0.319
2.141TyrAsn: 2.141 ± 0.317
1.456TyrPro: 1.456 ± 0.316
1.799TyrGln: 1.799 ± 0.312
2.056TyrArg: 2.056 ± 0.297
1.97TyrSer: 1.97 ± 0.478
2.227TyrThr: 2.227 ± 0.495
1.97TyrVal: 1.97 ± 0.302
0.257TyrTrp: 0.257 ± 0.143
1.542TyrTyr: 1.542 ± 0.337
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (11676 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski