Fly associated circular virus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; unclassified Porprismacovirus

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BPB3|A0A346BPB3_9VIRU Putative capsid protein OS=Fly associated circular virus 3 OX=2293283 PE=4 SV=1
MM1 pKa = 8.09AYY3 pKa = 10.04RR4 pKa = 11.84KK5 pKa = 9.62RR6 pKa = 11.84RR7 pKa = 11.84GSKK10 pKa = 9.55VLHH13 pKa = 6.49QKK15 pKa = 8.52FQWFFDD21 pKa = 3.94LQTKK25 pKa = 9.57ADD27 pKa = 3.7EE28 pKa = 4.12MQIIEE33 pKa = 4.4VSAGGYY39 pKa = 9.27GVYY42 pKa = 10.2KK43 pKa = 10.55RR44 pKa = 11.84LFPFFSAFKK53 pKa = 8.75YY54 pKa = 10.37YY55 pKa = 10.95KK56 pKa = 10.56LGGVKK61 pKa = 9.37MKK63 pKa = 9.33MIPASTLPVDD73 pKa = 3.87PTGLSYY79 pKa = 10.68EE80 pKa = 4.23AGEE83 pKa = 4.26NTVDD87 pKa = 4.85PRR89 pKa = 11.84DD90 pKa = 3.48QLTPGLTRR98 pKa = 11.84ITNGEE103 pKa = 4.0DD104 pKa = 3.58VYY106 pKa = 11.46TKK108 pKa = 10.95LDD110 pKa = 3.79GLTGDD115 pKa = 3.77QQRR118 pKa = 11.84EE119 pKa = 4.43LYY121 pKa = 9.6EE122 pKa = 4.92SMMIDD127 pKa = 3.25QRR129 pKa = 11.84WFKK132 pKa = 10.13WQLQSGLSRR141 pKa = 11.84YY142 pKa = 8.4ARR144 pKa = 11.84PMYY147 pKa = 8.75WQIGQLHH154 pKa = 6.12QDD156 pKa = 3.76YY157 pKa = 11.0LPGSVRR163 pKa = 11.84NLADD167 pKa = 3.42TNLTEE172 pKa = 4.73DD173 pKa = 4.56CDD175 pKa = 4.77SISAVYY181 pKa = 10.52DD182 pKa = 3.37GANPSGPSAGPITVAADD199 pKa = 3.17ASDD202 pKa = 3.55PRR204 pKa = 11.84GLFQVGHH211 pKa = 6.51RR212 pKa = 11.84GKK214 pKa = 10.35LGWMPTDD221 pKa = 3.49GVLLKK226 pKa = 9.81GTSGGDD232 pKa = 3.59GTVHH236 pKa = 5.98AQAVEE241 pKa = 3.92AAIPAVNVFTIVTPPMHH258 pKa = 6.15KK259 pKa = 8.5TNYY262 pKa = 7.64YY263 pKa = 8.29YY264 pKa = 10.56RR265 pKa = 11.84VFVTEE270 pKa = 4.34DD271 pKa = 3.84VYY273 pKa = 11.28FKK275 pKa = 11.2SPVVVGYY282 pKa = 10.0QNFRR286 pKa = 11.84SLDD289 pKa = 3.48RR290 pKa = 11.84FVQPQFPVAKK300 pKa = 9.97LPTVNSATTDD310 pKa = 3.39SNKK313 pKa = 9.75PFPTNDD319 pKa = 3.19GGEE322 pKa = 4.25LPGVIQQ328 pKa = 4.56

Molecular weight: 36.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BPB3|A0A346BPB3_9VIRU Putative capsid protein OS=Fly associated circular virus 3 OX=2293283 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 4.29AQRR5 pKa = 11.84RR6 pKa = 11.84YY7 pKa = 10.56KK8 pKa = 10.24FRR10 pKa = 11.84HH11 pKa = 4.91TLASTMTQTWMLTVPRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 7.48TAKK32 pKa = 9.2EE33 pKa = 3.31WMGIYY38 pKa = 9.82KK39 pKa = 9.73WLRR42 pKa = 11.84DD43 pKa = 3.58NDD45 pKa = 3.68VHH47 pKa = 6.67KK48 pKa = 9.25WTVAMEE54 pKa = 4.34TGSNGYY60 pKa = 8.07DD61 pKa = 2.53HH62 pKa = 6.6WQIRR66 pKa = 11.84FQVGKK71 pKa = 7.81TFKK74 pKa = 10.19QLKK77 pKa = 9.71KK78 pKa = 9.23EE79 pKa = 4.16WGDD82 pKa = 3.38KK83 pKa = 10.52AHH85 pKa = 7.06IEE87 pKa = 4.2EE88 pKa = 5.99ASDD91 pKa = 2.85TWDD94 pKa = 3.46YY95 pKa = 11.1EE96 pKa = 4.44RR97 pKa = 11.84KK98 pKa = 10.0SGMFFSSTDD107 pKa = 3.16TPEE110 pKa = 3.59VRR112 pKa = 11.84KK113 pKa = 10.24CRR115 pKa = 11.84FGRR118 pKa = 11.84LNWRR122 pKa = 11.84QEE124 pKa = 3.92AVVQAVQATNDD135 pKa = 3.52RR136 pKa = 11.84EE137 pKa = 4.43VVVWYY142 pKa = 10.18DD143 pKa = 3.17PDD145 pKa = 4.09GNKK148 pKa = 10.05GKK150 pKa = 10.18SWLLGHH156 pKa = 7.53LYY158 pKa = 8.31EE159 pKa = 5.2TGQAWVVQAQDD170 pKa = 3.45TVKK173 pKa = 11.21GIIQDD178 pKa = 3.64VASEE182 pKa = 4.4YY183 pKa = 10.39INHH186 pKa = 5.83GWRR189 pKa = 11.84PIVVIDD195 pKa = 5.32IPRR198 pKa = 11.84TWKK201 pKa = 7.87WTSDD205 pKa = 3.27LYY207 pKa = 11.39VAIEE211 pKa = 4.27RR212 pKa = 11.84IKK214 pKa = 11.05DD215 pKa = 3.64GLIKK219 pKa = 10.46DD220 pKa = 3.78PRR222 pKa = 11.84YY223 pKa = 9.95SSKK226 pKa = 9.35TVHH229 pKa = 5.5IRR231 pKa = 11.84GVKK234 pKa = 10.04VLVTCSTMPKK244 pKa = 9.65LDD246 pKa = 3.93KK247 pKa = 10.91LSADD251 pKa = 2.92RR252 pKa = 11.84WVIIDD257 pKa = 3.73LL258 pKa = 4.19

Molecular weight: 30.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

586

258

328

293.0

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.973 ± 0.323

0.512 ± 0.156

7.167 ± 0.346

3.925 ± 0.429

3.754 ± 0.844

7.85 ± 1.203

2.048 ± 0.393

4.437 ± 0.814

6.655 ± 1.106

6.485 ± 0.626

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.73 ± 0.01

2.901 ± 0.34

5.119 ± 1.422

5.29 ± 0.378

5.973 ± 0.823

5.461 ± 0.249

7.338 ± 0.245

8.532 ± 0.226

3.413 ± 1.419

4.437 ± 0.561
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski