Aquamicrobium phage P14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Aqualcavirus; Aquamicrobium virus P14

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L5C072|A0A1L5C072_9CAUD Putative DNA maturase A OS=Aquamicrobium phage P14 OX=1927013 GN=BB738_0460 PE=4 SV=1
MM1 pKa = 7.44TFEE4 pKa = 5.01CKK6 pKa = 10.45NLVYY10 pKa = 10.55SSQDD14 pKa = 2.66GTLIDD19 pKa = 5.06LEE21 pKa = 4.65VNHH24 pKa = 7.42PDD26 pKa = 3.66FGWVPFTASPDD37 pKa = 3.38DD38 pKa = 4.01SEE40 pKa = 4.36EE41 pKa = 3.94HH42 pKa = 5.95GRR44 pKa = 11.84AIYY47 pKa = 9.84AAAVAGEE54 pKa = 4.42FGPVAPYY61 pKa = 10.22DD62 pKa = 4.04GPSSEE67 pKa = 5.17EE68 pKa = 3.54IAAQTARR75 pKa = 11.84AQRR78 pKa = 11.84DD79 pKa = 3.56QLLVEE84 pKa = 4.22LDD86 pKa = 4.18GYY88 pKa = 10.92VSNPLRR94 pKa = 11.84WASLAQEE101 pKa = 4.25QQGEE105 pKa = 4.37VAAYY109 pKa = 9.25RR110 pKa = 11.84QALLDD115 pKa = 3.79VPQQAGFPLGIDD127 pKa = 3.61WPAKK131 pKa = 9.5PAFLVV136 pKa = 3.57

Molecular weight: 14.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L5C032|A0A1L5C032_9CAUD Uncharacterized protein OS=Aquamicrobium phage P14 OX=1927013 GN=BB738_0080 PE=4 SV=1
MM1 pKa = 7.77IKK3 pKa = 10.3RR4 pKa = 11.84IINIIKK10 pKa = 9.35PYY12 pKa = 9.59RR13 pKa = 11.84VHH15 pKa = 6.41HH16 pKa = 5.94TLDD19 pKa = 2.9NGRR22 pKa = 11.84TVTYY26 pKa = 9.72YY27 pKa = 10.52SWTWTDD33 pKa = 2.71ALSWVKK39 pKa = 10.33CHH41 pKa = 6.31GLQDD45 pKa = 3.51RR46 pKa = 11.84VRR48 pKa = 11.84VFKK51 pKa = 9.42LTPVFSCWKK60 pKa = 9.79LVASRR65 pKa = 4.22

Molecular weight: 7.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12335

37

1319

257.0

28.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.907 ± 0.624

0.827 ± 0.163

6.096 ± 0.172

6.461 ± 0.361

3.47 ± 0.162

7.523 ± 0.272

2.059 ± 0.153

4.589 ± 0.163

5.156 ± 0.358

8.739 ± 0.391

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.165

3.818 ± 0.192

4.24 ± 0.244

4.491 ± 0.334

6.137 ± 0.324

5.991 ± 0.268

6.024 ± 0.499

7.369 ± 0.336

1.403 ± 0.13

3.3 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski