Paracraurococcus sp. NE82

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Paracraurococcus; unclassified Paracraurococcus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5359 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4D8A5|A0A4R4D8A5_9PROT Cytochrome P450 OS=Paracraurococcus sp. NE82 OX=2527868 GN=EXY23_19725 PE=3 SV=1
MM1 pKa = 7.76IYY3 pKa = 10.67LNAVNSSLSTSASAVKK19 pKa = 9.59WLATSGTGSTLLGTTLNDD37 pKa = 3.51YY38 pKa = 11.04LSTSDD43 pKa = 3.45PTATLGGGKK52 pKa = 10.2GDD54 pKa = 3.4DD55 pKa = 3.52WYY57 pKa = 11.04AVSDD61 pKa = 3.8YY62 pKa = 9.84RR63 pKa = 11.84TTILEE68 pKa = 4.29TTGNGIDD75 pKa = 4.31TVQSWASRR83 pKa = 11.84FTLPAFVEE91 pKa = 4.01NLQLVGSNQLGTGNALNNIILAKK114 pKa = 9.63TGNATLDD121 pKa = 3.46GGAGNDD127 pKa = 3.48VLVGGTGADD136 pKa = 3.5TFVMSQGNGSDD147 pKa = 3.89AIYY150 pKa = 10.68SFNAAQDD157 pKa = 3.91TIKK160 pKa = 10.88LQGYY164 pKa = 7.07GQYY167 pKa = 11.14GFAAVKK173 pKa = 10.64AGMTQVGADD182 pKa = 3.47TVIALGGGEE191 pKa = 4.52KK192 pKa = 10.42LVLKK196 pKa = 10.33NVQASSLTAQNFSLPSDD213 pKa = 3.59PAHH216 pKa = 7.09FGRR219 pKa = 11.84AMTFGDD225 pKa = 4.01EE226 pKa = 4.25FDD228 pKa = 4.17SLSASGSGLGTDD240 pKa = 4.63WKK242 pKa = 7.78TTWKK246 pKa = 10.28IGVQGRR252 pKa = 11.84TFGTASDD259 pKa = 3.36QAYY262 pKa = 10.67YY263 pKa = 11.25GDD265 pKa = 4.83ASTGTNPFSIEE276 pKa = 4.08DD277 pKa = 4.47GILGITASPGGAAGMGYY294 pKa = 8.81TSGVLTTAKK303 pKa = 10.39SFAQLYY309 pKa = 10.33GYY311 pKa = 9.77FEE313 pKa = 5.09ISAQMPVGEE322 pKa = 4.95GYY324 pKa = 10.88LPAFWLLPTTGAWPPEE340 pKa = 3.64IDD342 pKa = 3.0IFEE345 pKa = 4.41YY346 pKa = 10.36VAKK349 pKa = 10.34DD350 pKa = 3.08PTAIYY355 pKa = 9.52TSYY358 pKa = 9.59GTTAGGATYY367 pKa = 8.39RR368 pKa = 11.84TAHH371 pKa = 6.63LSDD374 pKa = 4.36LSSGFHH380 pKa = 6.47TYY382 pKa = 10.27GLSWQADD389 pKa = 3.54LMKK392 pKa = 10.03WYY394 pKa = 10.94VDD396 pKa = 3.4DD397 pKa = 3.83TLVYY401 pKa = 10.09QVATPAEE408 pKa = 4.7LKK410 pKa = 10.34QPMYY414 pKa = 10.17MLVSLLTGTANSWQGLPTAANPTGTLQVDD443 pKa = 3.56YY444 pKa = 11.2VRR446 pKa = 11.84AYY448 pKa = 10.48QSTAVNVDD456 pKa = 3.32DD457 pKa = 5.53AGDD460 pKa = 3.45VAALGGHH467 pKa = 5.08YY468 pKa = 8.84TRR470 pKa = 11.84NADD473 pKa = 4.37GSDD476 pKa = 3.67LYY478 pKa = 11.44DD479 pKa = 3.78FTDD482 pKa = 3.54SAAMLVMDD490 pKa = 5.46ASGLSSGLHH499 pKa = 5.34GVLAGAAGSQVRR511 pKa = 11.84GSAGPMSFTGGAGVDD526 pKa = 3.43SFFFGVGNATVSGGDD541 pKa = 3.62GNDD544 pKa = 3.19SFVLTRR550 pKa = 11.84GVIAAGDD557 pKa = 3.89VIGDD561 pKa = 3.54FHH563 pKa = 8.55LDD565 pKa = 3.29LGNGTEE571 pKa = 4.27HH572 pKa = 7.5DD573 pKa = 3.97QLQLAGFSGDD583 pKa = 2.95AHH585 pKa = 7.76LDD587 pKa = 3.59VVSASGSTQIYY598 pKa = 9.08RR599 pKa = 11.84VVDD602 pKa = 3.66GTYY605 pKa = 9.86VSPNITIAVVNGAAHH620 pKa = 7.35LSAADD625 pKa = 3.6YY626 pKa = 11.57AFVV629 pKa = 3.54

Molecular weight: 64.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2WLY4|A0A4V2WLY4_9PROT Corrinoid adenosyltransferase OS=Paracraurococcus sp. NE82 OX=2527868 GN=EXY23_04115 PE=3 SV=1
MM1 pKa = 7.36SGSARR6 pKa = 11.84ARR8 pKa = 11.84QPPKK12 pKa = 9.93PRR14 pKa = 11.84PAPRR18 pKa = 11.84AAPKK22 pKa = 10.01SAPRR26 pKa = 11.84QPARR30 pKa = 11.84AAPPALSGWRR40 pKa = 11.84RR41 pKa = 11.84WRR43 pKa = 11.84WVAVLVLAAVLGLPLAHH60 pKa = 7.0AMFGEE65 pKa = 4.9VVALLGGTFLLGLLVGRR82 pKa = 11.84WTARR86 pKa = 3.15

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5359

0

5359

1709467

25

2561

319.0

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.185 ± 0.054

0.881 ± 0.01

4.921 ± 0.027

5.74 ± 0.031

3.119 ± 0.02

9.542 ± 0.036

2.022 ± 0.016

3.806 ± 0.022

1.845 ± 0.021

11.158 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.015

1.869 ± 0.017

6.445 ± 0.036

2.975 ± 0.02

8.783 ± 0.032

4.096 ± 0.021

4.836 ± 0.021

7.306 ± 0.03

1.52 ± 0.015

1.723 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski