His1 virus (isolate Australia/Victoria) (His1V) (Haloarcula hispanica virus 1)

Taxonomy: Viruses; Halspiviridae; Salterprovirus; Salterprovirus His1

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q25BG7|Y028_HIS1I Putative transmembrane protein ORF28 OS=His1 virus (isolate Australia/Victoria) OX=654912 GN=ORF28 PE=4 SV=1
MM1 pKa = 8.1SEE3 pKa = 5.03EE4 pKa = 4.15YY5 pKa = 10.46LQSDD9 pKa = 4.52LARR12 pKa = 11.84AIEE15 pKa = 4.04THH17 pKa = 6.35SIEE20 pKa = 5.18SVEE23 pKa = 4.11NLNKK27 pKa = 10.36SEE29 pKa = 4.22TVFYY33 pKa = 10.44ICDD36 pKa = 3.6VLGVEE41 pKa = 4.89PPEE44 pKa = 4.15AHH46 pKa = 6.6PSEE49 pKa = 4.91HH50 pKa = 6.58NGTVPRR56 pKa = 11.84SLSQEE61 pKa = 3.6WAKK64 pKa = 10.73ILAEE68 pKa = 3.9EE69 pKa = 4.41AEE71 pKa = 4.5EE72 pKa = 4.25NSEE75 pKa = 4.17EE76 pKa = 4.17NNDD79 pKa = 3.91EE80 pKa = 4.35SEE82 pKa = 4.14EE83 pKa = 4.28DD84 pKa = 3.4NN85 pKa = 4.45

Molecular weight: 9.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q25BH2|Y023_HIS1I Putative transmembrane protein ORF23 OS=His1 virus (isolate Australia/Victoria) OX=654912 GN=ORF23 PE=3 SV=1
MM1 pKa = 7.28MLQTAFTDD9 pKa = 4.22LANPSYY15 pKa = 11.43LNMGLALLLATIMVMILWAGMRR37 pKa = 11.84LKK39 pKa = 10.84SPAVFVIWALTSITLIFTFVTQFSFIWFWVMVMLSLLLISIVASIRR85 pKa = 11.84YY86 pKa = 6.45TLL88 pKa = 3.59

Molecular weight: 10.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

4432

15

717

126.6

14.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.912 ± 0.299

1.715 ± 0.342

5.438 ± 0.506

7.491 ± 0.752

4.129 ± 0.428

6.453 ± 0.395

1.376 ± 0.224

7.062 ± 0.575

6.137 ± 0.651

8.258 ± 0.528

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.339 ± 0.487

5.551 ± 0.524

3.294 ± 0.254

3.61 ± 0.243

3.542 ± 0.442

6.972 ± 0.482

6.724 ± 0.588

6.927 ± 0.56

1.895 ± 0.189

4.174 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski