Leifsonia rubra CMS 76R

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia; Leifsonia rubra

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2670 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S7XEY2|S7XEY2_9MICO Integral membrane protein OS=Leifsonia rubra CMS 76R OX=1348338 GN=ADILRU_0026 PE=4 SV=1
MM1 pKa = 7.61ISHH4 pKa = 7.69HH5 pKa = 5.69YY6 pKa = 9.62RR7 pKa = 11.84RR8 pKa = 11.84AIVPIAVLGIAGMVLSGCATSTGGTGDD35 pKa = 3.11TSGPGDD41 pKa = 3.77AGEE44 pKa = 4.5ADD46 pKa = 4.64GIVTIYY52 pKa = 9.5GTISDD57 pKa = 4.34TEE59 pKa = 4.2AEE61 pKa = 4.19LLEE64 pKa = 4.37EE65 pKa = 5.22SWAEE69 pKa = 3.79WEE71 pKa = 4.35TEE73 pKa = 3.51NDD75 pKa = 2.7IDD77 pKa = 4.35IQYY80 pKa = 8.53EE81 pKa = 3.79ASKK84 pKa = 10.59EE85 pKa = 3.86FEE87 pKa = 4.06AQVSIRR93 pKa = 11.84AQGGNAPDD101 pKa = 4.05LAIFPQPGLLADD113 pKa = 4.51LASRR117 pKa = 11.84DD118 pKa = 4.11YY119 pKa = 10.79IQPAPEE125 pKa = 3.95GVASNVEE132 pKa = 4.75EE133 pKa = 4.3YY134 pKa = 10.25WSDD137 pKa = 2.94DD138 pKa = 2.83WAAYY142 pKa = 10.32ASTGDD147 pKa = 3.8TLYY150 pKa = 10.87GAPLMASVKK159 pKa = 10.16GFVWYY164 pKa = 10.49SPSQFAEE171 pKa = 3.94WGVDD175 pKa = 4.26VPTTWDD181 pKa = 3.08EE182 pKa = 4.12MLTLTEE188 pKa = 5.05TIASTTGSAPWCVGFGSDD206 pKa = 5.73AATGWPGTDD215 pKa = 3.24WIEE218 pKa = 4.3DD219 pKa = 3.61LVLRR223 pKa = 11.84EE224 pKa = 4.88AGPEE228 pKa = 4.18VYY230 pKa = 10.08DD231 pKa = 2.99QWVSHH236 pKa = 7.07EE237 pKa = 4.63IPFTDD242 pKa = 4.24PAIKK246 pKa = 10.33SAFDD250 pKa = 3.46SVGEE254 pKa = 3.83ILLNPEE260 pKa = 3.81YY261 pKa = 11.21VNAGFGGVEE270 pKa = 4.71SINTTPFGDD279 pKa = 3.69PARR282 pKa = 11.84ALGDD286 pKa = 3.74GTCALHH292 pKa = 6.19HH293 pKa = 5.88QASFYY298 pKa = 11.17DD299 pKa = 5.1GFIQDD304 pKa = 4.55PKK306 pKa = 10.92NGNATVGPDD315 pKa = 2.95ADD317 pKa = 2.71IWAFVTPSIEE327 pKa = 4.03AGGNAVTGGGEE338 pKa = 3.86IVGAFSNDD346 pKa = 2.91EE347 pKa = 3.84DD348 pKa = 4.31TIAVQEE354 pKa = 4.19YY355 pKa = 10.23LSSAEE360 pKa = 3.92WANSRR365 pKa = 11.84VSLGGVISANNGLDD379 pKa = 3.56PTSASSPILQEE390 pKa = 5.12AITILQDD397 pKa = 3.06PSTTFRR403 pKa = 11.84FDD405 pKa = 4.43ASDD408 pKa = 3.64LMPGVVGAGAFWTGMVDD425 pKa = 3.84WINGKK430 pKa = 8.31STDD433 pKa = 4.4DD434 pKa = 3.5VLSTIDD440 pKa = 3.86ASWPSEE446 pKa = 3.75

Molecular weight: 47.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S7VXX8|S7VXX8_9MICO Transcriptional regulator LacI family OS=Leifsonia rubra CMS 76R OX=1348338 GN=ADILRU_2495 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.33VRR4 pKa = 11.84NSLKK8 pKa = 10.26ALKK11 pKa = 9.44KK12 pKa = 10.27IPGAQIVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84TFVINKK30 pKa = 9.03KK31 pKa = 8.56NPRR34 pKa = 11.84MKK36 pKa = 10.47ARR38 pKa = 11.84QGG40 pKa = 3.24

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2670

0

2670

799840

37

2128

299.6

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.356 ± 0.068

0.503 ± 0.01

5.841 ± 0.044

5.779 ± 0.047

3.348 ± 0.027

8.35 ± 0.043

2.001 ± 0.025

5.367 ± 0.036

2.53 ± 0.038

10.164 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.935 ± 0.02

2.617 ± 0.028

4.873 ± 0.033

2.977 ± 0.023

6.5 ± 0.047

6.553 ± 0.043

6.24 ± 0.045

8.656 ± 0.046

1.371 ± 0.022

2.038 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski