Flavobacterium sp. Leaf359

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5UQM2|A0A0Q5UQM2_9FLAO Uncharacterized protein OS=Flavobacterium sp. Leaf359 OX=1736351 GN=ASG38_14940 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 9.4QTGIEE7 pKa = 3.99LFEE10 pKa = 4.42YY11 pKa = 9.17LTSLPEE17 pKa = 3.74FTAVMGTRR25 pKa = 11.84LFPLVSDD32 pKa = 4.13SDD34 pKa = 4.14TPFPFAIYY42 pKa = 9.9GVRR45 pKa = 11.84AFPGSKK51 pKa = 10.44DD52 pKa = 3.18SDD54 pKa = 3.84SYY56 pKa = 11.6EE57 pKa = 4.11ITLSAYY63 pKa = 9.88FGPNAYY69 pKa = 9.77DD70 pKa = 3.43EE71 pKa = 4.79CAGFCDD77 pKa = 3.68MLRR80 pKa = 11.84PLLEE84 pKa = 5.18DD85 pKa = 3.16KK86 pKa = 11.29YY87 pKa = 10.7GWTDD91 pKa = 3.09SSVDD95 pKa = 3.61VAEE98 pKa = 4.59EE99 pKa = 4.46DD100 pKa = 3.92YY101 pKa = 11.82SFIGTINLKK110 pKa = 7.43TQQ112 pKa = 2.92

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5UM88|A0A0Q5UM88_9FLAO Homogentisate 1 2-dioxygenase OS=Flavobacterium sp. Leaf359 OX=1736351 GN=ASG38_02815 PE=4 SV=1
MM1 pKa = 6.67GTGRR5 pKa = 11.84AGRR8 pKa = 11.84SEE10 pKa = 4.08AAAQEE15 pKa = 4.23GMRR18 pKa = 11.84ARR20 pKa = 11.84EE21 pKa = 3.66FHH23 pKa = 7.29LYY25 pKa = 9.04GQAPGFSLGMLYY37 pKa = 10.62LRR39 pKa = 11.84DD40 pKa = 3.41APRR43 pKa = 11.84RR44 pKa = 11.84GGKK47 pKa = 7.31EE48 pKa = 3.77LRR50 pKa = 11.84VMSLEE55 pKa = 4.06SHH57 pKa = 6.86GGPIVCISDD66 pKa = 3.42HH67 pKa = 6.06AYY69 pKa = 9.38KK70 pKa = 10.58RR71 pKa = 11.84ARR73 pKa = 11.84EE74 pKa = 3.78RR75 pKa = 11.84LGLNGRR81 pKa = 11.84SFGRR85 pKa = 11.84LARR88 pKa = 11.84KK89 pKa = 9.63AFADD93 pKa = 5.38GIRR96 pKa = 11.84HH97 pKa = 5.58KK98 pKa = 7.96QTKK101 pKa = 9.54GALKK105 pKa = 10.26RR106 pKa = 11.84FIDD109 pKa = 3.93RR110 pKa = 11.84LHH112 pKa = 6.17LRR114 pKa = 11.84NRR116 pKa = 11.84AVDD119 pKa = 3.67NIRR122 pKa = 11.84IYY124 pKa = 11.07GEE126 pKa = 3.96HH127 pKa = 6.62VYY129 pKa = 10.58LFQGIFLVTVYY140 pKa = 10.24RR141 pKa = 11.84VPTEE145 pKa = 4.08LIKK148 pKa = 10.56YY149 pKa = 9.53LKK151 pKa = 10.41YY152 pKa = 10.68FKK154 pKa = 10.57QQ155 pKa = 3.41

Molecular weight: 17.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3309

0

3309

1096483

46

2628

331.4

37.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.631 ± 0.049

0.745 ± 0.017

5.308 ± 0.035

6.558 ± 0.055

5.319 ± 0.036

6.395 ± 0.045

1.654 ± 0.017

7.896 ± 0.04

7.953 ± 0.066

9.124 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.22 ± 0.021

6.201 ± 0.041

3.453 ± 0.027

3.45 ± 0.022

3.394 ± 0.028

6.511 ± 0.034

5.954 ± 0.071

6.094 ± 0.032

0.991 ± 0.013

4.149 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski