Lachancea mirantina

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Lachancea

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5053 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G4J9J0|A0A1G4J9J0_9SACH LAMI_0D02168g1_1 OS=Lachancea mirantina OX=1230905 GN=LAMI_0D02168G PE=4 SV=1
MM1 pKa = 7.62LSSQQIVALTALVGLAQASWWDD23 pKa = 3.54GFLNPNATSSSSTAPAVVSSSQATDD48 pKa = 3.38LASASSQSSAVASTAPAIQPYY69 pKa = 9.85ISSSSQGAQATLSPISSEE87 pKa = 4.08TSSEE91 pKa = 4.05GGQTTLSPTSSDD103 pKa = 3.27VSSEE107 pKa = 4.37DD108 pKa = 3.52GSQSFQAYY116 pKa = 9.81LSSIMQATQTQGSSAFTVYY135 pKa = 10.1PSSSSYY141 pKa = 11.19SSPQDD146 pKa = 3.37TTTLAPTFSADD157 pKa = 3.15SFTSATSTTSDD168 pKa = 2.71WNTFSSSEE176 pKa = 3.93QSLTPSSEE184 pKa = 4.36SKK186 pKa = 11.04LEE188 pKa = 4.15SQLSQSASFSTSAWGFSNTRR208 pKa = 11.84STPVEE213 pKa = 3.89KK214 pKa = 10.81SFSSSSALASEE225 pKa = 4.66SATLATPQNTILDD238 pKa = 4.03SSEE241 pKa = 4.36SPTASEE247 pKa = 4.69PTTSAPKK254 pKa = 7.83WTSSASPEE262 pKa = 4.51SVTSAAPDD270 pKa = 3.27ATKK273 pKa = 10.49PILSEE278 pKa = 4.37SASSTGPIPSSEE290 pKa = 4.13ASWLSSEE297 pKa = 5.09QYY299 pKa = 9.34TATQRR304 pKa = 11.84EE305 pKa = 4.68VTTASATPSSANVAASSVEE324 pKa = 3.93IQSEE328 pKa = 4.52VQSASSFGSFSASEE342 pKa = 4.14TSSTEE347 pKa = 3.84VDD349 pKa = 3.0SSTALQAKK357 pKa = 9.81SSPQTSTWPASTAVSLSIVPTSSSEE382 pKa = 4.29SQVSSALSVTSSSLTPASAAPSSSEE407 pKa = 3.52FSIEE411 pKa = 3.86QSSSQFEE418 pKa = 4.36DD419 pKa = 3.54TSITGVDD426 pKa = 3.87WNTVSYY432 pKa = 9.2PASSSAADD440 pKa = 3.68VPSSTQYY447 pKa = 11.2SSQQAQPTSQSPLWSSSGAQKK468 pKa = 9.71TYY470 pKa = 10.79SYY472 pKa = 11.22VPSTSSSSYY481 pKa = 10.84DD482 pKa = 3.17SSTAEE487 pKa = 3.99SWASTPASQSSASDD501 pKa = 2.88ISNYY505 pKa = 10.44AEE507 pKa = 3.99TWQTASSSASPSSYY521 pKa = 10.72LPSSSNYY528 pKa = 9.93AGSTSSYY535 pKa = 10.47PYY537 pKa = 9.15LTSSQIYY544 pKa = 7.97ATSSSDD550 pKa = 3.24SYY552 pKa = 11.78EE553 pKa = 4.15SFPPTSSLASSEE565 pKa = 4.06YY566 pKa = 9.61STLSSEE572 pKa = 4.51ASLLSTSSQSAFSVTPSASSEE593 pKa = 4.15SSFTSTVPAPSSLSASSFVTDD614 pKa = 3.82DD615 pKa = 4.11FSRR618 pKa = 11.84SSSSLPTSSSAATQSTTSLMVSSSQSTSDD647 pKa = 3.3STSIASVSSFPEE659 pKa = 4.12SSVSTPAPSSLVTVSSYY676 pKa = 11.45ASEE679 pKa = 4.2NATSSALIASLSSSPSASITSTAEE703 pKa = 3.56SSEE706 pKa = 3.97FSSALFTSSYY716 pKa = 10.25NQTDD720 pKa = 3.59TGSLASSASTEE731 pKa = 4.32SVSEE735 pKa = 4.27SLFSATVSATSSFEE749 pKa = 4.28SSVSTPIAIPSSATSALTTGAPEE772 pKa = 3.85QVTSSEE778 pKa = 4.31VAQNSSSLGSSYY790 pKa = 10.99TSSTSNWLPTAIITAGGDD808 pKa = 3.65TQSTGSGAAASGGQQATQTLPQAIAAATAVSQPKK842 pKa = 10.15DD843 pKa = 3.48YY844 pKa = 10.49TLITIGFKK852 pKa = 10.44QEE854 pKa = 3.5LNYY857 pKa = 10.4PFVVKK862 pKa = 10.83NSLTSAQIFAYY873 pKa = 10.44LPGVLNLPFEE883 pKa = 4.98DD884 pKa = 5.24SFDD887 pKa = 3.91QIQVVQLSPLNVKK900 pKa = 9.43GRR902 pKa = 11.84SYY904 pKa = 11.19LSTVAEE910 pKa = 4.7VYY912 pKa = 10.45FPSSEE917 pKa = 3.73VDD919 pKa = 3.1ALQEE923 pKa = 3.81LVQNSEE929 pKa = 4.08SKK931 pKa = 10.88LYY933 pKa = 10.92LSANSALAGLIDD945 pKa = 3.63TTIPLTGLVQSSDD958 pKa = 3.26GSSDD962 pKa = 3.48DD963 pKa = 3.85TSSQSSTSQSNSEE976 pKa = 4.25GNTDD980 pKa = 3.03STGGSRR986 pKa = 11.84GRR988 pKa = 11.84IAGTLDD994 pKa = 2.99VTYY997 pKa = 10.6NASASSKK1004 pKa = 9.91GQPGNNGKK1012 pKa = 9.55LVGLIIGVVVGAIVYY1027 pKa = 8.94LAFMVVGYY1035 pKa = 10.48RR1036 pKa = 11.84FFVLKK1041 pKa = 10.36RR1042 pKa = 11.84KK1043 pKa = 8.29QQAHH1047 pKa = 6.22SNDD1050 pKa = 3.8LGDD1053 pKa = 4.17GQSLGSSTDD1062 pKa = 3.16HH1063 pKa = 7.24GSYY1066 pKa = 10.64LYY1068 pKa = 10.35DD1069 pKa = 3.45EE1070 pKa = 5.41KK1071 pKa = 11.58EE1072 pKa = 4.13NGDD1075 pKa = 3.89QVSLSPSMRR1084 pKa = 11.84INNWMRR1090 pKa = 11.84HH1091 pKa = 3.8NHH1093 pKa = 5.19YY1094 pKa = 9.59EE1095 pKa = 3.99YY1096 pKa = 10.89SGEE1099 pKa = 4.09GQQNLGEE1106 pKa = 4.17QHH1108 pKa = 6.64SSQPKK1113 pKa = 7.83ISRR1116 pKa = 11.84PIATEE1121 pKa = 4.26NSLGWNDD1128 pKa = 3.22VV1129 pKa = 3.19

Molecular weight: 115.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G4JQ71|A0A1G4JQ71_9SACH Enhancer of translation termination 1 OS=Lachancea mirantina OX=1230905 GN=LAMI_0E12618G PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.11WRR6 pKa = 11.84KK7 pKa = 9.07KK8 pKa = 6.93RR9 pKa = 11.84TRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.48RR15 pKa = 11.84KK16 pKa = 8.41RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5053

0

5053

2449895

25

4908

484.8

54.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.917 ± 0.029

1.346 ± 0.012

5.783 ± 0.023

6.632 ± 0.029

4.502 ± 0.02

5.676 ± 0.027

2.181 ± 0.012

5.505 ± 0.022

6.408 ± 0.033

9.887 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.048 ± 0.011

4.691 ± 0.017

4.437 ± 0.026

4.135 ± 0.028

5.253 ± 0.022

8.715 ± 0.04

5.45 ± 0.025

6.277 ± 0.023

1.101 ± 0.011

3.057 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski