Amino acid dipepetide frequency for Microbacterium phage Busephilis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.27AlaAla: 17.27 ± 1.429
0.831AlaCys: 0.831 ± 0.26
8.19AlaAsp: 8.19 ± 0.977
7.953AlaGlu: 7.953 ± 0.852
3.323AlaPhe: 3.323 ± 0.38
8.843AlaGly: 8.843 ± 0.782
2.315AlaHis: 2.315 ± 0.369
5.282AlaIle: 5.282 ± 0.618
3.501AlaLys: 3.501 ± 0.561
11.632AlaLeu: 11.632 ± 1.116
3.264AlaMet: 3.264 ± 0.456
3.027AlaAsn: 3.027 ± 0.357
6.409AlaPro: 6.409 ± 0.681
4.51AlaGln: 4.51 ± 0.594
7.003AlaArg: 7.003 ± 0.744
7.24AlaSer: 7.24 ± 0.794
7.953AlaThr: 7.953 ± 0.738
6.944AlaVal: 6.944 ± 0.728
2.967AlaTrp: 2.967 ± 0.442
2.611AlaTyr: 2.611 ± 0.436
0.0AlaXaa: 0.0 ± 0.0
Cys
0.653CysAla: 0.653 ± 0.213
0.297CysCys: 0.297 ± 0.182
1.128CysAsp: 1.128 ± 0.307
0.653CysGlu: 0.653 ± 0.192
0.237CysPhe: 0.237 ± 0.113
1.306CysGly: 1.306 ± 0.262
0.356CysHis: 0.356 ± 0.118
0.237CysIle: 0.237 ± 0.116
0.0CysLys: 0.0 ± 0.0
0.356CysLeu: 0.356 ± 0.144
0.178CysMet: 0.178 ± 0.106
0.178CysAsn: 0.178 ± 0.107
0.89CysPro: 0.89 ± 0.22
0.237CysGln: 0.237 ± 0.104
0.534CysArg: 0.534 ± 0.174
0.534CysSer: 0.534 ± 0.29
0.534CysThr: 0.534 ± 0.162
0.712CysVal: 0.712 ± 0.225
0.119CysTrp: 0.119 ± 0.088
0.119CysTyr: 0.119 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
8.427AspAla: 8.427 ± 0.883
0.653AspCys: 0.653 ± 0.25
4.926AspAsp: 4.926 ± 0.762
5.104AspGlu: 5.104 ± 0.771
1.899AspPhe: 1.899 ± 0.289
6.528AspGly: 6.528 ± 0.603
1.246AspHis: 1.246 ± 0.285
2.849AspIle: 2.849 ± 0.429
1.246AspLys: 1.246 ± 0.279
5.519AspLeu: 5.519 ± 0.517
1.721AspMet: 1.721 ± 0.379
1.602AspAsn: 1.602 ± 0.338
4.095AspPro: 4.095 ± 0.549
2.315AspGln: 2.315 ± 0.395
5.104AspArg: 5.104 ± 0.723
3.264AspSer: 3.264 ± 0.508
2.967AspThr: 2.967 ± 0.403
4.985AspVal: 4.985 ± 0.442
0.89AspTrp: 0.89 ± 0.281
1.78AspTyr: 1.78 ± 0.363
0.0AspXaa: 0.0 ± 0.0
Glu
8.249GluAla: 8.249 ± 0.917
0.475GluCys: 0.475 ± 0.162
4.807GluAsp: 4.807 ± 0.761
3.917GluGlu: 3.917 ± 0.658
1.602GluPhe: 1.602 ± 0.315
5.104GluGly: 5.104 ± 0.598
1.662GluHis: 1.662 ± 0.355
1.84GluIle: 1.84 ± 0.489
1.721GluLys: 1.721 ± 0.332
3.145GluLeu: 3.145 ± 0.402
1.246GluMet: 1.246 ± 0.254
1.543GluAsn: 1.543 ± 0.34
4.688GluPro: 4.688 ± 0.802
2.374GluGln: 2.374 ± 0.474
5.757GluArg: 5.757 ± 0.651
2.908GluSer: 2.908 ± 0.498
3.739GluThr: 3.739 ± 0.541
6.053GluVal: 6.053 ± 0.72
1.484GluTrp: 1.484 ± 0.33
2.136GluTyr: 2.136 ± 0.37
0.0GluXaa: 0.0 ± 0.0
Phe
3.264PheAla: 3.264 ± 0.481
0.237PheCys: 0.237 ± 0.15
2.315PheAsp: 2.315 ± 0.405
1.899PheGlu: 1.899 ± 0.31
0.712PhePhe: 0.712 ± 0.209
2.493PheGly: 2.493 ± 0.392
0.356PheHis: 0.356 ± 0.134
1.306PheIle: 1.306 ± 0.312
0.772PheLys: 0.772 ± 0.22
1.543PheLeu: 1.543 ± 0.291
0.534PheMet: 0.534 ± 0.17
0.356PheAsn: 0.356 ± 0.149
1.068PhePro: 1.068 ± 0.254
0.593PheGln: 0.593 ± 0.189
1.246PheArg: 1.246 ± 0.249
1.187PheSer: 1.187 ± 0.295
2.374PheThr: 2.374 ± 0.281
1.78PheVal: 1.78 ± 0.355
0.237PheTrp: 0.237 ± 0.107
0.772PheTyr: 0.772 ± 0.246
0.0PheXaa: 0.0 ± 0.0
Gly
8.19GlyAla: 8.19 ± 0.883
0.653GlyCys: 0.653 ± 0.203
5.935GlyAsp: 5.935 ± 0.593
5.697GlyGlu: 5.697 ± 0.531
2.611GlyPhe: 2.611 ± 0.433
7.3GlyGly: 7.3 ± 0.701
2.018GlyHis: 2.018 ± 0.4
4.273GlyIle: 4.273 ± 0.582
2.611GlyLys: 2.611 ± 0.426
6.706GlyLeu: 6.706 ± 1.05
3.442GlyMet: 3.442 ± 0.48
1.899GlyAsn: 1.899 ± 0.331
3.442GlyPro: 3.442 ± 0.435
3.145GlyGln: 3.145 ± 0.449
5.697GlyArg: 5.697 ± 0.538
5.045GlySer: 5.045 ± 0.617
6.113GlyThr: 6.113 ± 0.549
5.994GlyVal: 5.994 ± 0.534
2.493GlyTrp: 2.493 ± 0.413
2.967GlyTyr: 2.967 ± 0.38
0.0GlyXaa: 0.0 ± 0.0
His
1.958HisAla: 1.958 ± 0.316
0.178HisCys: 0.178 ± 0.1
1.424HisAsp: 1.424 ± 0.273
1.484HisGlu: 1.484 ± 0.271
0.534HisPhe: 0.534 ± 0.214
1.958HisGly: 1.958 ± 0.421
0.356HisHis: 0.356 ± 0.181
0.831HisIle: 0.831 ± 0.214
0.475HisLys: 0.475 ± 0.159
2.018HisLeu: 2.018 ± 0.421
0.356HisMet: 0.356 ± 0.147
0.534HisAsn: 0.534 ± 0.174
1.543HisPro: 1.543 ± 0.314
0.356HisGln: 0.356 ± 0.134
1.009HisArg: 1.009 ± 0.278
0.95HisSer: 0.95 ± 0.241
1.306HisThr: 1.306 ± 0.286
1.484HisVal: 1.484 ± 0.292
0.356HisTrp: 0.356 ± 0.158
0.89HisTyr: 0.89 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
4.332IleAla: 4.332 ± 0.359
0.415IleCys: 0.415 ± 0.186
3.383IleAsp: 3.383 ± 0.485
3.68IleGlu: 3.68 ± 0.401
0.831IlePhe: 0.831 ± 0.186
3.383IleGly: 3.383 ± 0.422
0.712IleHis: 0.712 ± 0.212
1.84IleIle: 1.84 ± 0.477
1.009IleLys: 1.009 ± 0.213
2.611IleLeu: 2.611 ± 0.391
0.95IleMet: 0.95 ± 0.261
1.068IleAsn: 1.068 ± 0.271
3.323IlePro: 3.323 ± 0.689
1.246IleGln: 1.246 ± 0.306
3.442IleArg: 3.442 ± 0.514
2.255IleSer: 2.255 ± 0.407
4.57IleThr: 4.57 ± 0.551
4.036IleVal: 4.036 ± 0.422
0.653IleTrp: 0.653 ± 0.186
0.653IleTyr: 0.653 ± 0.196
0.0IleXaa: 0.0 ± 0.0
Lys
3.798LysAla: 3.798 ± 0.537
0.356LysCys: 0.356 ± 0.163
1.009LysAsp: 1.009 ± 0.261
0.593LysGlu: 0.593 ± 0.204
0.772LysPhe: 0.772 ± 0.223
2.374LysGly: 2.374 ± 0.39
0.593LysHis: 0.593 ± 0.185
1.009LysIle: 1.009 ± 0.22
0.593LysLys: 0.593 ± 0.199
1.009LysLeu: 1.009 ± 0.262
0.653LysMet: 0.653 ± 0.198
0.237LysAsn: 0.237 ± 0.112
1.958LysPro: 1.958 ± 0.411
0.593LysGln: 0.593 ± 0.168
2.433LysArg: 2.433 ± 0.446
1.78LysSer: 1.78 ± 0.301
1.602LysThr: 1.602 ± 0.335
2.967LysVal: 2.967 ± 0.493
0.475LysTrp: 0.475 ± 0.171
0.356LysTyr: 0.356 ± 0.134
0.0LysXaa: 0.0 ± 0.0
Leu
9.911LeuAla: 9.911 ± 0.913
0.297LeuCys: 0.297 ± 0.138
4.807LeuAsp: 4.807 ± 0.564
4.51LeuGlu: 4.51 ± 0.566
1.543LeuPhe: 1.543 ± 0.362
6.113LeuGly: 6.113 ± 1.051
1.128LeuHis: 1.128 ± 0.24
3.976LeuIle: 3.976 ± 0.642
0.653LeuLys: 0.653 ± 0.188
5.697LeuLeu: 5.697 ± 0.699
1.958LeuMet: 1.958 ± 0.317
1.662LeuAsn: 1.662 ± 0.372
4.807LeuPro: 4.807 ± 0.559
2.136LeuGln: 2.136 ± 0.36
4.748LeuArg: 4.748 ± 0.667
5.875LeuSer: 5.875 ± 0.593
6.588LeuThr: 6.588 ± 0.571
6.113LeuVal: 6.113 ± 0.553
1.128LeuTrp: 1.128 ± 0.286
1.187LeuTyr: 1.187 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
3.205MetAla: 3.205 ± 0.429
0.178MetCys: 0.178 ± 0.103
1.128MetAsp: 1.128 ± 0.272
0.415MetGlu: 0.415 ± 0.159
0.415MetPhe: 0.415 ± 0.154
2.196MetGly: 2.196 ± 0.401
0.534MetHis: 0.534 ± 0.171
1.958MetIle: 1.958 ± 0.403
0.297MetLys: 0.297 ± 0.131
1.662MetLeu: 1.662 ± 0.342
0.356MetMet: 0.356 ± 0.153
0.712MetAsn: 0.712 ± 0.187
1.424MetPro: 1.424 ± 0.303
0.712MetGln: 0.712 ± 0.202
1.78MetArg: 1.78 ± 0.294
2.611MetSer: 2.611 ± 0.348
2.552MetThr: 2.552 ± 0.318
1.662MetVal: 1.662 ± 0.295
0.356MetTrp: 0.356 ± 0.155
0.772MetTyr: 0.772 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
3.383AsnAla: 3.383 ± 0.414
0.237AsnCys: 0.237 ± 0.108
1.009AsnAsp: 1.009 ± 0.213
0.89AsnGlu: 0.89 ± 0.251
0.356AsnPhe: 0.356 ± 0.192
3.086AsnGly: 3.086 ± 0.472
0.415AsnHis: 0.415 ± 0.163
0.653AsnIle: 0.653 ± 0.196
0.297AsnLys: 0.297 ± 0.156
2.611AsnLeu: 2.611 ± 0.324
0.356AsnMet: 0.356 ± 0.127
0.593AsnAsn: 0.593 ± 0.195
2.611AsnPro: 2.611 ± 0.481
0.534AsnGln: 0.534 ± 0.192
2.077AsnArg: 2.077 ± 0.382
1.602AsnSer: 1.602 ± 0.309
2.018AsnThr: 2.018 ± 0.334
1.484AsnVal: 1.484 ± 0.332
0.178AsnTrp: 0.178 ± 0.101
0.475AsnTyr: 0.475 ± 0.161
0.0AsnXaa: 0.0 ± 0.0
Pro
7.834ProAla: 7.834 ± 0.935
0.772ProCys: 0.772 ± 0.25
4.629ProAsp: 4.629 ± 0.597
5.875ProGlu: 5.875 ± 0.86
1.484ProPhe: 1.484 ± 0.293
5.401ProGly: 5.401 ± 0.468
1.246ProHis: 1.246 ± 0.311
1.543ProIle: 1.543 ± 0.287
1.543ProLys: 1.543 ± 0.296
4.036ProLeu: 4.036 ± 0.475
1.424ProMet: 1.424 ± 0.295
1.662ProAsn: 1.662 ± 0.374
4.095ProPro: 4.095 ± 0.63
1.721ProGln: 1.721 ± 0.433
2.789ProArg: 2.789 ± 0.356
3.798ProSer: 3.798 ± 0.484
4.51ProThr: 4.51 ± 0.779
4.095ProVal: 4.095 ± 0.576
0.831ProTrp: 0.831 ± 0.226
1.602ProTyr: 1.602 ± 0.315
0.0ProXaa: 0.0 ± 0.0
Gln
3.976GlnAla: 3.976 ± 0.546
0.297GlnCys: 0.297 ± 0.11
2.018GlnAsp: 2.018 ± 0.376
1.246GlnGlu: 1.246 ± 0.223
1.306GlnPhe: 1.306 ± 0.278
2.552GlnGly: 2.552 ± 0.409
0.89GlnHis: 0.89 ± 0.203
1.721GlnIle: 1.721 ± 0.299
0.831GlnLys: 0.831 ± 0.258
1.128GlnLeu: 1.128 ± 0.331
1.187GlnMet: 1.187 ± 0.239
1.128GlnAsn: 1.128 ± 0.257
1.721GlnPro: 1.721 ± 0.323
1.128GlnGln: 1.128 ± 0.277
2.255GlnArg: 2.255 ± 0.426
2.018GlnSer: 2.018 ± 0.339
2.136GlnThr: 2.136 ± 0.362
2.493GlnVal: 2.493 ± 0.442
0.653GlnTrp: 0.653 ± 0.166
1.068GlnTyr: 1.068 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
6.884ArgAla: 6.884 ± 0.664
0.89ArgCys: 0.89 ± 0.23
4.51ArgAsp: 4.51 ± 0.588
4.926ArgGlu: 4.926 ± 0.747
1.662ArgPhe: 1.662 ± 0.369
6.053ArgGly: 6.053 ± 0.573
1.662ArgHis: 1.662 ± 0.38
2.789ArgIle: 2.789 ± 0.367
2.196ArgLys: 2.196 ± 0.355
6.053ArgLeu: 6.053 ± 0.587
1.721ArgMet: 1.721 ± 0.357
1.365ArgAsn: 1.365 ± 0.299
3.205ArgPro: 3.205 ± 0.402
3.027ArgGln: 3.027 ± 0.401
7.24ArgArg: 7.24 ± 0.853
3.264ArgSer: 3.264 ± 0.418
3.68ArgThr: 3.68 ± 0.516
4.688ArgVal: 4.688 ± 0.576
1.662ArgTrp: 1.662 ± 0.32
2.433ArgTyr: 2.433 ± 0.426
0.0ArgXaa: 0.0 ± 0.0
Ser
8.012SerAla: 8.012 ± 1.172
0.178SerCys: 0.178 ± 0.097
4.926SerAsp: 4.926 ± 0.646
3.323SerGlu: 3.323 ± 0.5
1.662SerPhe: 1.662 ± 0.385
4.866SerGly: 4.866 ± 0.656
1.306SerHis: 1.306 ± 0.313
3.086SerIle: 3.086 ± 0.492
1.78SerLys: 1.78 ± 0.324
4.451SerLeu: 4.451 ± 0.511
1.84SerMet: 1.84 ± 0.284
1.899SerAsn: 1.899 ± 0.317
2.611SerPro: 2.611 ± 0.426
1.365SerGln: 1.365 ± 0.297
3.501SerArg: 3.501 ± 0.538
2.493SerSer: 2.493 ± 0.523
4.392SerThr: 4.392 ± 0.483
4.451SerVal: 4.451 ± 0.533
1.009SerTrp: 1.009 ± 0.205
1.246SerTyr: 1.246 ± 0.341
0.0SerXaa: 0.0 ± 0.0
Thr
8.309ThrAla: 8.309 ± 0.769
0.772ThrCys: 0.772 ± 0.243
3.976ThrAsp: 3.976 ± 0.548
4.451ThrGlu: 4.451 ± 0.512
1.365ThrPhe: 1.365 ± 0.32
6.766ThrGly: 6.766 ± 0.738
1.484ThrHis: 1.484 ± 0.371
3.323ThrIle: 3.323 ± 0.495
2.018ThrLys: 2.018 ± 0.447
5.579ThrLeu: 5.579 ± 0.593
1.246ThrMet: 1.246 ± 0.243
1.365ThrAsn: 1.365 ± 0.299
5.816ThrPro: 5.816 ± 0.687
1.543ThrGln: 1.543 ± 0.286
4.154ThrArg: 4.154 ± 0.443
3.798ThrSer: 3.798 ± 0.53
5.163ThrThr: 5.163 ± 0.541
6.766ThrVal: 6.766 ± 0.584
0.89ThrTrp: 0.89 ± 0.212
1.899ThrTyr: 1.899 ± 0.291
0.0ThrXaa: 0.0 ± 0.0
Val
8.368ValAla: 8.368 ± 0.73
0.95ValCys: 0.95 ± 0.237
3.561ValAsp: 3.561 ± 0.554
4.985ValGlu: 4.985 ± 0.517
1.424ValPhe: 1.424 ± 0.277
6.231ValGly: 6.231 ± 0.673
1.128ValHis: 1.128 ± 0.24
3.976ValIle: 3.976 ± 0.475
2.315ValLys: 2.315 ± 0.38
5.697ValLeu: 5.697 ± 0.49
1.662ValMet: 1.662 ± 0.346
2.671ValAsn: 2.671 ± 0.409
5.223ValPro: 5.223 ± 0.585
2.374ValGln: 2.374 ± 0.469
5.163ValArg: 5.163 ± 0.588
4.688ValSer: 4.688 ± 0.589
6.053ValThr: 6.053 ± 0.451
5.045ValVal: 5.045 ± 0.562
1.602ValTrp: 1.602 ± 0.367
2.255ValTyr: 2.255 ± 0.38
0.0ValXaa: 0.0 ± 0.0
Trp
2.136TrpAla: 2.136 ± 0.367
0.475TrpCys: 0.475 ± 0.163
0.89TrpAsp: 0.89 ± 0.257
1.187TrpGlu: 1.187 ± 0.269
0.89TrpPhe: 0.89 ± 0.227
1.246TrpGly: 1.246 ± 0.242
0.356TrpHis: 0.356 ± 0.135
0.772TrpIle: 0.772 ± 0.223
0.653TrpLys: 0.653 ± 0.191
1.484TrpLeu: 1.484 ± 0.312
0.178TrpMet: 0.178 ± 0.091
0.712TrpAsn: 0.712 ± 0.21
0.772TrpPro: 0.772 ± 0.233
0.712TrpGln: 0.712 ± 0.199
1.721TrpArg: 1.721 ± 0.321
1.246TrpSer: 1.246 ± 0.283
0.831TrpThr: 0.831 ± 0.21
1.602TrpVal: 1.602 ± 0.26
0.356TrpTrp: 0.356 ± 0.131
0.653TrpTyr: 0.653 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.908TyrAla: 2.908 ± 0.536
0.119TyrCys: 0.119 ± 0.101
2.552TyrAsp: 2.552 ± 0.423
1.662TyrGlu: 1.662 ± 0.316
0.415TyrPhe: 0.415 ± 0.192
2.493TyrGly: 2.493 ± 0.39
0.237TyrHis: 0.237 ± 0.112
1.128TyrIle: 1.128 ± 0.205
0.653TyrLys: 0.653 ± 0.207
1.899TyrLeu: 1.899 ± 0.349
0.593TyrMet: 0.593 ± 0.217
0.653TyrAsn: 0.653 ± 0.192
1.187TyrPro: 1.187 ± 0.294
1.128TyrGln: 1.128 ± 0.26
2.255TyrArg: 2.255 ± 0.309
1.84TyrSer: 1.84 ± 0.38
1.602TyrThr: 1.602 ± 0.313
2.018TyrVal: 2.018 ± 0.349
0.534TyrTrp: 0.534 ± 0.164
0.89TyrTyr: 0.89 ± 0.262
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (16851 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski