Thermoflexus hugenholtzii JAD2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Thermoflexia; Thermoflexales; Thermoflexaceae; Thermoflexus; Thermoflexus hugenholtzii

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A212RL63|A0A212RL63_9CHLR Glyoxalase family protein OS=Thermoflexus hugenholtzii JAD2 OX=877466 GN=SAMN02746019_00017180 PE=4 SV=1
MM1 pKa = 7.18SQAICPEE8 pKa = 4.21CAAGVPLRR16 pKa = 11.84EE17 pKa = 3.91DD18 pKa = 3.48VMLHH22 pKa = 6.2EE23 pKa = 5.51FLTCPEE29 pKa = 4.81CGTALEE35 pKa = 4.52VVSLSPLTLDD45 pKa = 3.87RR46 pKa = 11.84APQVEE51 pKa = 4.6EE52 pKa = 4.15DD53 pKa = 3.18WGEE56 pKa = 3.71

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A212RJZ2|A0A212RJZ2_9CHLR 2-alkenal reductase OS=Thermoflexus hugenholtzii JAD2 OX=877466 GN=SAMN02746019_00016390 PE=4 SV=1
PP1 pKa = 7.11GLSRR5 pKa = 11.84GNRR8 pKa = 11.84PPLRR12 pKa = 11.84RR13 pKa = 11.84TRR15 pKa = 11.84RR16 pKa = 11.84SPAPTLRR23 pKa = 11.84RR24 pKa = 11.84VRR26 pKa = 11.84RR27 pKa = 11.84PPPGVPGLSRR37 pKa = 11.84GNRR40 pKa = 11.84PPLRR44 pKa = 11.84RR45 pKa = 3.6

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2944

0

2944

955847

26

2049

324.7

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.639 ± 0.055

0.74 ± 0.014

4.19 ± 0.031

7.171 ± 0.054

3.495 ± 0.028

8.538 ± 0.048

1.976 ± 0.018

4.975 ± 0.035

1.755 ± 0.034

11.968 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.017

1.657 ± 0.022

6.992 ± 0.044

3.025 ± 0.027

9.44 ± 0.049

3.826 ± 0.025

4.436 ± 0.033

7.387 ± 0.039

2.109 ± 0.031

2.527 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski