Amino acid dipepetide frequency for Escherichia phage anhysbys

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.879AlaAla: 4.879 ± 0.409
0.783AlaCys: 0.783 ± 0.139
3.313AlaAsp: 3.313 ± 0.274
5.125AlaGlu: 5.125 ± 0.416
2.708AlaPhe: 2.708 ± 0.248
4.991AlaGly: 4.991 ± 0.367
1.388AlaHis: 1.388 ± 0.161
4.118AlaIle: 4.118 ± 0.294
5.103AlaLys: 5.103 ± 0.369
5.998AlaLeu: 5.998 ± 0.36
2.014AlaMet: 2.014 ± 0.197
3.76AlaAsn: 3.76 ± 0.247
2.059AlaPro: 2.059 ± 0.215
2.171AlaGln: 2.171 ± 0.2
2.663AlaArg: 2.663 ± 0.284
3.939AlaSer: 3.939 ± 0.293
4.566AlaThr: 4.566 ± 0.368
4.745AlaVal: 4.745 ± 0.291
1.253AlaTrp: 1.253 ± 0.179
3.447AlaTyr: 3.447 ± 0.278
0.0AlaXaa: 0.0 ± 0.0
Cys
0.94CysAla: 0.94 ± 0.138
0.246CysCys: 0.246 ± 0.071
0.806CysAsp: 0.806 ± 0.159
1.074CysGlu: 1.074 ± 0.168
1.209CysPhe: 1.209 ± 0.181
0.94CysGly: 0.94 ± 0.141
0.313CysHis: 0.313 ± 0.071
0.671CysIle: 0.671 ± 0.133
1.119CysLys: 1.119 ± 0.164
1.097CysLeu: 1.097 ± 0.144
0.582CysMet: 0.582 ± 0.112
0.515CysAsn: 0.515 ± 0.131
0.627CysPro: 0.627 ± 0.152
0.448CysGln: 0.448 ± 0.104
0.56CysArg: 0.56 ± 0.135
0.739CysSer: 0.739 ± 0.127
0.873CysThr: 0.873 ± 0.141
1.186CysVal: 1.186 ± 0.152
0.291CysTrp: 0.291 ± 0.095
0.537CysTyr: 0.537 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
4.006AspAla: 4.006 ± 0.286
0.851AspCys: 0.851 ± 0.136
3.805AspAsp: 3.805 ± 0.276
4.364AspGlu: 4.364 ± 0.36
3.133AspPhe: 3.133 ± 0.279
4.723AspGly: 4.723 ± 0.341
1.321AspHis: 1.321 ± 0.179
3.939AspIle: 3.939 ± 0.285
3.962AspLys: 3.962 ± 0.3
5.573AspLeu: 5.573 ± 0.412
1.656AspMet: 1.656 ± 0.18
3.536AspAsn: 3.536 ± 0.27
2.372AspPro: 2.372 ± 0.202
2.171AspGln: 2.171 ± 0.207
2.462AspArg: 2.462 ± 0.207
3.245AspSer: 3.245 ± 0.31
3.201AspThr: 3.201 ± 0.288
4.7AspVal: 4.7 ± 0.312
1.253AspTrp: 1.253 ± 0.158
3.492AspTyr: 3.492 ± 0.28
0.0AspXaa: 0.0 ± 0.0
Glu
5.64GluAla: 5.64 ± 0.351
0.962GluCys: 0.962 ± 0.152
5.707GluAsp: 5.707 ± 0.418
8.416GluGlu: 8.416 ± 0.611
2.977GluPhe: 2.977 ± 0.305
4.588GluGly: 4.588 ± 0.31
1.276GluHis: 1.276 ± 0.184
4.476GluIle: 4.476 ± 0.321
5.081GluLys: 5.081 ± 0.344
5.707GluLeu: 5.707 ± 0.388
2.708GluMet: 2.708 ± 0.25
3.962GluAsn: 3.962 ± 0.289
1.925GluPro: 1.925 ± 0.221
2.641GluGln: 2.641 ± 0.224
2.887GluArg: 2.887 ± 0.257
3.492GluSer: 3.492 ± 0.271
4.185GluThr: 4.185 ± 0.229
4.767GluVal: 4.767 ± 0.373
1.858GluTrp: 1.858 ± 0.221
3.268GluTyr: 3.268 ± 0.28
0.0GluXaa: 0.0 ± 0.0
Phe
3.111PheAla: 3.111 ± 0.262
0.627PheCys: 0.627 ± 0.101
3.178PheAsp: 3.178 ± 0.253
3.402PheGlu: 3.402 ± 0.322
2.126PhePhe: 2.126 ± 0.23
2.798PheGly: 2.798 ± 0.276
0.716PheHis: 0.716 ± 0.139
2.328PheIle: 2.328 ± 0.241
3.201PheLys: 3.201 ± 0.245
3.402PheLeu: 3.402 ± 0.251
1.141PheMet: 1.141 ± 0.17
2.82PheAsn: 2.82 ± 0.244
1.746PhePro: 1.746 ± 0.164
1.365PheGln: 1.365 ± 0.16
1.723PheArg: 1.723 ± 0.208
2.954PheSer: 2.954 ± 0.29
2.552PheThr: 2.552 ± 0.238
2.999PheVal: 2.999 ± 0.267
0.649PheTrp: 0.649 ± 0.131
2.35PheTyr: 2.35 ± 0.223
0.0PheXaa: 0.0 ± 0.0
Gly
4.029GlyAla: 4.029 ± 0.319
1.231GlyCys: 1.231 ± 0.223
4.32GlyAsp: 4.32 ± 0.416
5.014GlyGlu: 5.014 ± 0.275
3.245GlyPhe: 3.245 ± 0.317
4.096GlyGly: 4.096 ± 0.319
1.343GlyHis: 1.343 ± 0.173
4.141GlyIle: 4.141 ± 0.322
4.991GlyLys: 4.991 ± 0.357
4.946GlyLeu: 4.946 ± 0.334
1.768GlyMet: 1.768 ± 0.195
3.447GlyAsn: 3.447 ± 0.301
0.94GlyPro: 0.94 ± 0.201
1.634GlyGln: 1.634 ± 0.241
2.596GlyArg: 2.596 ± 0.252
3.648GlySer: 3.648 ± 0.3
3.917GlyThr: 3.917 ± 0.481
5.081GlyVal: 5.081 ± 0.339
1.209GlyTrp: 1.209 ± 0.196
3.671GlyTyr: 3.671 ± 0.277
0.0GlyXaa: 0.0 ± 0.0
His
0.94HisAla: 0.94 ± 0.163
0.425HisCys: 0.425 ± 0.086
1.03HisAsp: 1.03 ± 0.162
1.164HisGlu: 1.164 ± 0.197
1.052HisPhe: 1.052 ± 0.163
1.365HisGly: 1.365 ± 0.188
0.336HisHis: 0.336 ± 0.099
1.186HisIle: 1.186 ± 0.146
1.253HisLys: 1.253 ± 0.187
1.343HisLeu: 1.343 ± 0.163
0.671HisMet: 0.671 ± 0.128
1.007HisAsn: 1.007 ± 0.153
0.806HisPro: 0.806 ± 0.142
0.47HisGln: 0.47 ± 0.115
0.873HisArg: 0.873 ± 0.165
0.873HisSer: 0.873 ± 0.145
0.985HisThr: 0.985 ± 0.159
1.432HisVal: 1.432 ± 0.191
0.313HisTrp: 0.313 ± 0.087
1.141HisTyr: 1.141 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
4.678IleAla: 4.678 ± 0.372
1.097IleCys: 1.097 ± 0.164
3.984IleAsp: 3.984 ± 0.267
4.544IleGlu: 4.544 ± 0.325
2.395IlePhe: 2.395 ± 0.231
3.581IleGly: 3.581 ± 0.29
1.007IleHis: 1.007 ± 0.138
3.76IleIle: 3.76 ± 0.314
3.984IleLys: 3.984 ± 0.302
4.745IleLeu: 4.745 ± 0.351
1.477IleMet: 1.477 ± 0.181
3.671IleAsn: 3.671 ± 0.33
2.999IlePro: 2.999 ± 0.318
2.216IleGln: 2.216 ± 0.223
2.574IleArg: 2.574 ± 0.229
3.402IleSer: 3.402 ± 0.349
4.163IleThr: 4.163 ± 0.281
4.364IleVal: 4.364 ± 0.319
0.627IleTrp: 0.627 ± 0.129
2.44IleTyr: 2.44 ± 0.247
0.0IleXaa: 0.0 ± 0.0
Lys
5.595LysAla: 5.595 ± 0.423
0.582LysCys: 0.582 ± 0.121
4.32LysAsp: 4.32 ± 0.36
6.021LysGlu: 6.021 ± 0.36
2.35LysPhe: 2.35 ± 0.218
4.521LysGly: 4.521 ± 0.489
1.611LysHis: 1.611 ± 0.172
4.32LysIle: 4.32 ± 0.285
4.409LysLys: 4.409 ± 0.461
4.342LysLeu: 4.342 ± 0.371
2.731LysMet: 2.731 ± 0.289
3.402LysAsn: 3.402 ± 0.285
2.059LysPro: 2.059 ± 0.233
2.552LysGln: 2.552 ± 0.278
3.066LysArg: 3.066 ± 0.297
3.559LysSer: 3.559 ± 0.255
3.939LysThr: 3.939 ± 0.287
4.588LysVal: 4.588 ± 0.384
0.985LysTrp: 0.985 ± 0.152
2.663LysTyr: 2.663 ± 0.239
0.0LysXaa: 0.0 ± 0.0
Leu
5.998LeuAla: 5.998 ± 0.372
1.119LeuCys: 1.119 ± 0.158
5.551LeuAsp: 5.551 ± 0.309
6.222LeuGlu: 6.222 ± 0.362
3.066LeuPhe: 3.066 ± 0.305
4.924LeuGly: 4.924 ± 0.34
1.365LeuHis: 1.365 ± 0.194
4.364LeuIle: 4.364 ± 0.295
5.819LeuLys: 5.819 ± 0.337
5.819LeuLeu: 5.819 ± 0.338
2.238LeuMet: 2.238 ± 0.223
4.118LeuAsn: 4.118 ± 0.307
3.022LeuPro: 3.022 ± 0.275
2.462LeuGln: 2.462 ± 0.275
3.38LeuArg: 3.38 ± 0.298
5.775LeuSer: 5.775 ± 0.337
5.26LeuThr: 5.26 ± 0.324
5.014LeuVal: 5.014 ± 0.287
1.052LeuTrp: 1.052 ± 0.181
3.022LeuTyr: 3.022 ± 0.278
0.0LeuXaa: 0.0 ± 0.0
Met
2.372MetAla: 2.372 ± 0.247
0.358MetCys: 0.358 ± 0.081
1.388MetAsp: 1.388 ± 0.155
1.634MetGlu: 1.634 ± 0.211
1.164MetPhe: 1.164 ± 0.157
1.723MetGly: 1.723 ± 0.19
0.716MetHis: 0.716 ± 0.123
2.126MetIle: 2.126 ± 0.199
2.126MetLys: 2.126 ± 0.203
2.171MetLeu: 2.171 ± 0.224
0.716MetMet: 0.716 ± 0.135
1.007MetAsn: 1.007 ± 0.186
0.761MetPro: 0.761 ± 0.134
1.141MetGln: 1.141 ± 0.167
1.522MetArg: 1.522 ± 0.174
2.395MetSer: 2.395 ± 0.248
1.813MetThr: 1.813 ± 0.191
1.791MetVal: 1.791 ± 0.179
0.291MetTrp: 0.291 ± 0.08
0.94MetTyr: 0.94 ± 0.132
0.0MetXaa: 0.0 ± 0.0
Asn
3.693AsnAla: 3.693 ± 0.283
0.627AsnCys: 0.627 ± 0.107
2.753AsnAsp: 2.753 ± 0.21
2.954AsnGlu: 2.954 ± 0.295
2.193AsnPhe: 2.193 ± 0.25
4.253AsnGly: 4.253 ± 0.408
0.918AsnHis: 0.918 ± 0.162
4.23AsnIle: 4.23 ± 0.371
3.827AsnLys: 3.827 ± 0.283
4.342AsnLeu: 4.342 ± 0.337
1.388AsnMet: 1.388 ± 0.156
2.775AsnAsn: 2.775 ± 0.242
2.775AsnPro: 2.775 ± 0.229
1.365AsnGln: 1.365 ± 0.196
1.902AsnArg: 1.902 ± 0.219
3.38AsnSer: 3.38 ± 0.292
3.626AsnThr: 3.626 ± 0.25
3.245AsnVal: 3.245 ± 0.305
0.918AsnTrp: 0.918 ± 0.158
2.261AsnTyr: 2.261 ± 0.235
0.0AsnXaa: 0.0 ± 0.0
Pro
2.149ProAla: 2.149 ± 0.224
0.828ProCys: 0.828 ± 0.138
2.507ProAsp: 2.507 ± 0.263
3.201ProGlu: 3.201 ± 0.253
2.171ProPhe: 2.171 ± 0.205
1.858ProGly: 1.858 ± 0.248
0.671ProHis: 0.671 ± 0.13
1.656ProIle: 1.656 ± 0.222
1.947ProLys: 1.947 ± 0.242
2.193ProLeu: 2.193 ± 0.217
0.716ProMet: 0.716 ± 0.12
2.149ProAsn: 2.149 ± 0.277
0.94ProPro: 0.94 ± 0.133
0.985ProGln: 0.985 ± 0.163
1.209ProArg: 1.209 ± 0.158
2.395ProSer: 2.395 ± 0.263
2.35ProThr: 2.35 ± 0.24
2.82ProVal: 2.82 ± 0.238
0.47ProTrp: 0.47 ± 0.084
1.656ProTyr: 1.656 ± 0.191
0.0ProXaa: 0.0 ± 0.0
Gln
2.261GlnAla: 2.261 ± 0.196
0.47GlnCys: 0.47 ± 0.094
1.947GlnAsp: 1.947 ± 0.25
2.887GlnGlu: 2.887 ± 0.259
1.209GlnPhe: 1.209 ± 0.154
1.88GlnGly: 1.88 ± 0.265
0.537GlnHis: 0.537 ± 0.103
2.708GlnIle: 2.708 ± 0.201
1.522GlnLys: 1.522 ± 0.204
2.059GlnLeu: 2.059 ± 0.2
1.03GlnMet: 1.03 ± 0.169
1.746GlnAsn: 1.746 ± 0.222
1.141GlnPro: 1.141 ± 0.155
1.164GlnGln: 1.164 ± 0.163
1.321GlnArg: 1.321 ± 0.188
1.701GlnSer: 1.701 ± 0.174
1.723GlnThr: 1.723 ± 0.176
2.014GlnVal: 2.014 ± 0.21
0.604GlnTrp: 0.604 ± 0.121
1.343GlnTyr: 1.343 ± 0.178
0.0GlnXaa: 0.0 ± 0.0
Arg
2.574ArgAla: 2.574 ± 0.235
0.783ArgCys: 0.783 ± 0.147
3.022ArgAsp: 3.022 ± 0.272
2.731ArgGlu: 2.731 ± 0.361
1.791ArgPhe: 1.791 ± 0.21
2.753ArgGly: 2.753 ± 0.274
0.582ArgHis: 0.582 ± 0.11
2.686ArgIle: 2.686 ± 0.245
2.977ArgLys: 2.977 ± 0.234
3.38ArgLeu: 3.38 ± 0.304
1.276ArgMet: 1.276 ± 0.191
2.484ArgAsn: 2.484 ± 0.252
1.141ArgPro: 1.141 ± 0.17
1.477ArgGln: 1.477 ± 0.202
1.947ArgArg: 1.947 ± 0.251
2.529ArgSer: 2.529 ± 0.239
1.656ArgThr: 1.656 ± 0.218
2.708ArgVal: 2.708 ± 0.223
0.582ArgTrp: 0.582 ± 0.113
1.432ArgTyr: 1.432 ± 0.159
0.0ArgXaa: 0.0 ± 0.0
Ser
3.827SerAla: 3.827 ± 0.302
0.985SerCys: 0.985 ± 0.148
3.648SerAsp: 3.648 ± 0.281
3.805SerGlu: 3.805 ± 0.271
3.156SerPhe: 3.156 ± 0.297
3.894SerGly: 3.894 ± 0.349
1.03SerHis: 1.03 ± 0.172
3.156SerIle: 3.156 ± 0.291
3.939SerLys: 3.939 ± 0.307
5.484SerLeu: 5.484 ± 0.337
1.432SerMet: 1.432 ± 0.173
3.223SerAsn: 3.223 ± 0.264
2.305SerPro: 2.305 ± 0.216
1.902SerGln: 1.902 ± 0.25
2.686SerArg: 2.686 ± 0.213
3.492SerSer: 3.492 ± 0.405
3.268SerThr: 3.268 ± 0.311
4.253SerVal: 4.253 ± 0.333
0.783SerTrp: 0.783 ± 0.145
2.641SerTyr: 2.641 ± 0.254
0.0SerXaa: 0.0 ± 0.0
Thr
3.76ThrAla: 3.76 ± 0.306
0.716ThrCys: 0.716 ± 0.132
3.022ThrAsp: 3.022 ± 0.265
4.096ThrGlu: 4.096 ± 0.317
3.268ThrPhe: 3.268 ± 0.321
4.611ThrGly: 4.611 ± 0.364
0.94ThrHis: 0.94 ± 0.144
3.805ThrIle: 3.805 ± 0.303
4.275ThrLys: 4.275 ± 0.345
5.551ThrLeu: 5.551 ± 0.331
1.209ThrMet: 1.209 ± 0.155
2.865ThrAsn: 2.865 ± 0.236
2.887ThrPro: 2.887 ± 0.219
1.679ThrGln: 1.679 ± 0.211
1.925ThrArg: 1.925 ± 0.19
3.447ThrSer: 3.447 ± 0.33
4.051ThrThr: 4.051 ± 0.357
4.253ThrVal: 4.253 ± 0.374
0.94ThrTrp: 0.94 ± 0.148
2.641ThrTyr: 2.641 ± 0.207
0.0ThrXaa: 0.0 ± 0.0
Val
4.633ValAla: 4.633 ± 0.302
1.141ValCys: 1.141 ± 0.177
4.767ValAsp: 4.767 ± 0.323
5.618ValGlu: 5.618 ± 0.388
3.089ValPhe: 3.089 ± 0.271
4.32ValGly: 4.32 ± 0.361
1.186ValHis: 1.186 ± 0.142
4.588ValIle: 4.588 ± 0.326
4.7ValLys: 4.7 ± 0.374
5.752ValLeu: 5.752 ± 0.341
1.902ValMet: 1.902 ± 0.179
3.581ValAsn: 3.581 ± 0.289
2.082ValPro: 2.082 ± 0.205
1.589ValGln: 1.589 ± 0.197
2.507ValArg: 2.507 ± 0.234
4.074ValSer: 4.074 ± 0.293
4.051ValThr: 4.051 ± 0.306
6.312ValVal: 6.312 ± 0.465
0.851ValTrp: 0.851 ± 0.161
3.313ValTyr: 3.313 ± 0.296
0.0ValXaa: 0.0 ± 0.0
Trp
0.783TrpAla: 0.783 ± 0.136
0.246TrpCys: 0.246 ± 0.073
1.455TrpAsp: 1.455 ± 0.198
1.321TrpGlu: 1.321 ± 0.213
0.985TrpPhe: 0.985 ± 0.164
0.739TrpGly: 0.739 ± 0.152
0.448TrpHis: 0.448 ± 0.101
1.074TrpIle: 1.074 ± 0.179
0.828TrpLys: 0.828 ± 0.113
1.701TrpLeu: 1.701 ± 0.223
0.492TrpMet: 0.492 ± 0.104
0.783TrpAsn: 0.783 ± 0.128
0.313TrpPro: 0.313 ± 0.082
0.38TrpGln: 0.38 ± 0.097
0.761TrpArg: 0.761 ± 0.135
0.873TrpSer: 0.873 ± 0.147
0.806TrpThr: 0.806 ± 0.129
1.074TrpVal: 1.074 ± 0.158
0.336TrpTrp: 0.336 ± 0.085
0.515TrpTyr: 0.515 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.156TyrAla: 3.156 ± 0.258
0.694TyrCys: 0.694 ± 0.117
3.245TyrAsp: 3.245 ± 0.246
2.932TyrGlu: 2.932 ± 0.257
1.902TyrPhe: 1.902 ± 0.211
2.708TyrGly: 2.708 ± 0.26
0.962TyrHis: 0.962 ± 0.132
2.372TyrIle: 2.372 ± 0.234
2.552TyrLys: 2.552 ± 0.254
3.984TyrLeu: 3.984 ± 0.276
0.985TyrMet: 0.985 ± 0.162
2.574TyrAsn: 2.574 ± 0.24
1.97TyrPro: 1.97 ± 0.217
1.455TyrGln: 1.455 ± 0.168
1.947TyrArg: 1.947 ± 0.169
2.999TyrSer: 2.999 ± 0.294
2.954TyrThr: 2.954 ± 0.249
2.708TyrVal: 2.708 ± 0.24
0.649TyrTrp: 0.649 ± 0.118
2.082TyrTyr: 2.082 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 272 proteins (44680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski