Hubei myriapoda virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLZ2|A0A1L3KLZ2_9VIRU Uncharacterized protein OS=Hubei myriapoda virus 2 OX=1922931 PE=4 SV=1
MM1 pKa = 7.84ADD3 pKa = 2.96GRR5 pKa = 11.84APAVPDD11 pKa = 3.51AEE13 pKa = 4.06AAEE16 pKa = 4.22TGEE19 pKa = 4.47IISTQTVPPTVPASRR34 pKa = 11.84VASGPPPSEE43 pKa = 3.83IVQHH47 pKa = 6.05TGNANLIDD55 pKa = 4.37PNILNQNILSSTITWSVNQPQNSVLGTFYY84 pKa = 11.01VHH86 pKa = 7.08PSYY89 pKa = 10.97HH90 pKa = 6.87LYY92 pKa = 10.8LDD94 pKa = 3.73YY95 pKa = 11.12LSRR98 pKa = 11.84IYY100 pKa = 8.57YY101 pKa = 7.26TWSGSLQYY109 pKa = 10.23SVCVLGTGFHH119 pKa = 6.91AGKK122 pKa = 10.29IMLVRR127 pKa = 11.84APPGYY132 pKa = 10.79DD133 pKa = 3.68LDD135 pKa = 3.88SLSNIEE141 pKa = 4.23EE142 pKa = 4.2ATGLEE147 pKa = 4.22WMALDD152 pKa = 4.29PKK154 pKa = 10.86SIIPAEE160 pKa = 4.35LGLNDD165 pKa = 3.29YY166 pKa = 10.87RR167 pKa = 11.84GVNFHH172 pKa = 6.04MTSIPADD179 pKa = 3.62SRR181 pKa = 11.84VTAAATKK188 pKa = 9.81TFACPEE194 pKa = 4.39GGWGRR199 pKa = 11.84LKK201 pKa = 10.61HH202 pKa = 6.88DD203 pKa = 4.18PDD205 pKa = 3.71KK206 pKa = 11.44VRR208 pKa = 11.84SAVRR212 pKa = 11.84AGQDD216 pKa = 3.1VSSFFDD222 pKa = 4.03TIRR225 pKa = 11.84HH226 pKa = 5.44GGILQLRR233 pKa = 11.84VMGEE237 pKa = 4.37LKK239 pKa = 10.16TSSTGNMQIQILVFVKK255 pKa = 10.26PGPDD259 pKa = 3.4FQFSTLIPPTVKK271 pKa = 10.51VGPTPSQVPAVIASLLSSAVAVRR294 pKa = 11.84RR295 pKa = 11.84CSATLSPVRR304 pKa = 11.84TLLVVSASVTPNKK317 pKa = 9.26MWFYY321 pKa = 11.44GCASSQGDD329 pKa = 3.25WCHH332 pKa = 5.16NTRR335 pKa = 11.84SYY337 pKa = 8.6VTKK340 pKa = 10.64DD341 pKa = 3.27PIITHH346 pKa = 5.63SPEE349 pKa = 3.34ISLLRR354 pKa = 11.84FPTVAKK360 pKa = 10.48NDD362 pKa = 3.49FSGYY366 pKa = 8.7VCDD369 pKa = 4.52VRR371 pKa = 11.84GATGNEE377 pKa = 4.36PIAQIAPKK385 pKa = 10.26GDD387 pKa = 3.32LGKK390 pKa = 10.79VVDD393 pKa = 4.82PFDD396 pKa = 5.14CNVLILEE403 pKa = 4.52NVVEE407 pKa = 4.27ANEE410 pKa = 4.14IRR412 pKa = 11.84GTEE415 pKa = 3.83YY416 pKa = 10.85DD417 pKa = 3.85APDD420 pKa = 3.8SVWIAEE426 pKa = 4.4EE427 pKa = 3.93NLSADD432 pKa = 3.78RR433 pKa = 11.84SEE435 pKa = 4.59VATVVHH441 pKa = 6.18PVGMWEE447 pKa = 5.05LPDD450 pKa = 3.68QEE452 pKa = 5.84LVFADD457 pKa = 3.67SAFMKK462 pKa = 9.09DD463 pKa = 2.64TWHH466 pKa = 6.72RR467 pKa = 11.84TLKK470 pKa = 10.19WPVNSVIDD478 pKa = 4.09RR479 pKa = 11.84EE480 pKa = 4.52SMTAKK485 pKa = 10.53LNLNFMPANAPVCWRR500 pKa = 11.84AIRR503 pKa = 11.84PVALDD508 pKa = 3.46LFIKK512 pKa = 10.56KK513 pKa = 9.65EE514 pKa = 3.9YY515 pKa = 10.87LNLKK519 pKa = 8.95PATKK523 pKa = 9.99EE524 pKa = 4.18SVFLFCPIKK533 pKa = 10.68PGTLDD538 pKa = 3.3AASIEE543 pKa = 4.23TGKK546 pKa = 11.01YY547 pKa = 10.16GLPTYY552 pKa = 9.45QTDD555 pKa = 3.47DD556 pKa = 3.2VSRR559 pKa = 11.84YY560 pKa = 8.85LAYY563 pKa = 10.63GNAQGLLTSEE573 pKa = 4.44DD574 pKa = 3.39ALLFEE579 pKa = 5.79LYY581 pKa = 10.1SVPLGTPVRR590 pKa = 11.84YY591 pKa = 10.0LKK593 pKa = 9.95MYY595 pKa = 9.11SSGMITTNASDD606 pKa = 3.36TTLILDD612 pKa = 4.04KK613 pKa = 11.25SDD615 pKa = 4.5DD616 pKa = 3.6YY617 pKa = 11.89DD618 pKa = 3.46VRR620 pKa = 11.84FVQKK624 pKa = 9.94IAASAAIPALPTHH637 pKa = 5.91STWASQVHH645 pKa = 5.92IANVVGKK652 pKa = 10.18RR653 pKa = 11.84EE654 pKa = 3.78NAMLRR659 pKa = 11.84EE660 pKa = 4.33SVTALDD666 pKa = 4.49RR667 pKa = 11.84KK668 pKa = 10.24IEE670 pKa = 4.08LLTSLLRR677 pKa = 11.84KK678 pKa = 9.53

Molecular weight: 74.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLZ2|A0A1L3KLZ2_9VIRU Uncharacterized protein OS=Hubei myriapoda virus 2 OX=1922931 PE=4 SV=1
MM1 pKa = 7.69AFQYY5 pKa = 11.01SAFLKK10 pKa = 10.89LMTILQNLSDD20 pKa = 4.59DD21 pKa = 3.91GKK23 pKa = 11.02LYY25 pKa = 10.77KK26 pKa = 10.27PSNLRR31 pKa = 11.84LLFYY35 pKa = 9.7TSPPAFWTIEE45 pKa = 3.99WIRR48 pKa = 11.84TFGFPAWLFPVFGSLFPDD66 pKa = 4.2NIHH69 pKa = 6.43ALSSKK74 pKa = 10.37SYY76 pKa = 8.38WVPSFTSLFSQVAVFWSHH94 pKa = 6.3GFTIDD99 pKa = 5.16DD100 pKa = 3.43LHH102 pKa = 7.59KK103 pKa = 11.18YY104 pKa = 10.25SDD106 pKa = 3.65HH107 pKa = 6.23QLVAKK112 pKa = 10.22ILTNFPIVTPKK123 pKa = 10.46HH124 pKa = 6.49DD125 pKa = 4.37FVTTMYY131 pKa = 9.45TCVGFFRR138 pKa = 11.84LQITAHH144 pKa = 6.69EE145 pKa = 4.7DD146 pKa = 2.89ADD148 pKa = 3.55IVRR151 pKa = 11.84RR152 pKa = 11.84HH153 pKa = 6.39LLPLLHH159 pKa = 7.18PGTRR163 pKa = 11.84HH164 pKa = 6.08CGCVQCFPAIKK175 pKa = 9.82KK176 pKa = 10.63YY177 pKa = 10.42YY178 pKa = 9.89DD179 pKa = 3.4LRR181 pKa = 11.84RR182 pKa = 11.84DD183 pKa = 3.17SSNIFLKK190 pKa = 10.86GIPSVYY196 pKa = 9.88LKK198 pKa = 10.59HH199 pKa = 6.01HH200 pKa = 5.67TAHH203 pKa = 6.58PHH205 pKa = 5.04FHH207 pKa = 7.0PFDD210 pKa = 4.3AFTCAVPGALSRR222 pKa = 11.84RR223 pKa = 11.84VVAPPTFRR231 pKa = 11.84KK232 pKa = 9.62ILSLFYY238 pKa = 9.02TKK240 pKa = 10.35CYY242 pKa = 10.41GALQSLGRR250 pKa = 11.84EE251 pKa = 4.04VHH253 pKa = 5.73TSIDD257 pKa = 3.42ALSNPLCDD265 pKa = 3.46GLEE268 pKa = 4.04PQRR271 pKa = 11.84LEE273 pKa = 4.49DD274 pKa = 4.62LSDD277 pKa = 3.09THH279 pKa = 7.65EE280 pKa = 4.3EE281 pKa = 4.45VFCPAMRR288 pKa = 11.84YY289 pKa = 8.84FVVHH293 pKa = 6.6HH294 pKa = 6.77LMTTTEE300 pKa = 4.1RR301 pKa = 11.84QFALNMTWDD310 pKa = 3.6LFLNFFNIPIASVAVEE326 pKa = 3.96LSVSRR331 pKa = 11.84WKK333 pKa = 10.95VFSEE337 pKa = 4.18KK338 pKa = 9.73IVRR341 pKa = 11.84SFWVPEE347 pKa = 3.73EE348 pKa = 3.88EE349 pKa = 4.4LYY351 pKa = 11.19EE352 pKa = 4.31FIIFANHH359 pKa = 6.67LCVCPGDD366 pKa = 4.99FIGEE370 pKa = 4.17PNFVKK375 pKa = 10.89FMDD378 pKa = 3.68AVTRR382 pKa = 11.84VCIQLSVLINLRR394 pKa = 11.84IALCSFVHH402 pKa = 6.9ADD404 pKa = 3.57VLADD408 pKa = 3.9TAALASRR415 pKa = 11.84IEE417 pKa = 4.36DD418 pKa = 3.8NEE420 pKa = 3.84WTPNIFFQLFRR431 pKa = 11.84TKK433 pKa = 9.68QQPRR437 pKa = 11.84SLMQLSHH444 pKa = 6.58MATMGEE450 pKa = 4.51FISTHH455 pKa = 5.99SEE457 pKa = 3.56MSEE460 pKa = 4.03NPDD463 pKa = 3.03MDD465 pKa = 3.84VVGG468 pKa = 4.14

Molecular weight: 53.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3244

468

2098

1081.3

121.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.905 ± 0.655

1.819 ± 0.204

6.196 ± 0.393

5.21 ± 0.461

5.025 ± 1.002

5.333 ± 0.475

2.682 ± 0.501

5.949 ± 0.199

4.84 ± 0.503

9.895 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.149

4.192 ± 0.283

5.425 ± 0.681

3.422 ± 0.297

5.456 ± 0.502

7.398 ± 0.429

5.672 ± 0.64

7.429 ± 0.373

1.541 ± 0.16

3.113 ± 0.083
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski