Amino acid dipepetide frequency for Ruminococcaceae bacterium AM28-23LB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.477AlaAla: 9.477 ± 0.145	1.467AlaCys: 1.467 ± 0.057	4.1AlaAsp: 4.1 ± 0.077	5.259AlaGlu: 5.259 ± 0.078	3.557AlaPhe: 3.557 ± 0.064	6.822AlaGly: 6.822 ± 0.103	1.466AlaHis: 1.466 ± 0.044	4.959AlaIle: 4.959 ± 0.081	3.918AlaLys: 3.918 ± 0.07	10.003AlaLeu: 10.003 ± 0.148	2.778AlaMet: 2.778 ± 0.054	2.116AlaAsn: 2.116 ± 0.06	3.566AlaPro: 3.566 ± 0.079	4.247AlaGln: 4.247 ± 0.075	4.538AlaArg: 4.538 ± 0.084	4.046AlaSer: 4.046 ± 0.076	3.232AlaThr: 3.232 ± 0.076	6.452AlaVal: 6.452 ± 0.096	0.806AlaTrp: 0.806 ± 0.032	2.526AlaTyr: 2.526 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.497CysAla: 1.497 ± 0.044	0.351CysCys: 0.351 ± 0.025	0.937CysAsp: 0.937 ± 0.035	0.771CysGlu: 0.771 ± 0.032	0.691CysPhe: 0.691 ± 0.025	1.756CysGly: 1.756 ± 0.048	0.408CysHis: 0.408 ± 0.022	0.856CysIle: 0.856 ± 0.033	0.732CysLys: 0.732 ± 0.035	1.457CysLeu: 1.457 ± 0.047	0.415CysMet: 0.415 ± 0.022	0.451CysAsn: 0.451 ± 0.024	0.94CysPro: 0.94 ± 0.039	0.819CysGln: 0.819 ± 0.031	1.272CysArg: 1.272 ± 0.044	0.982CysSer: 0.982 ± 0.032	0.862CysThr: 0.862 ± 0.033	1.035CysVal: 1.035 ± 0.035	0.157CysTrp: 0.157 ± 0.015	0.533CysTyr: 0.533 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	4.254AspAla: 4.254 ± 0.081	0.859AspCys: 0.859 ± 0.031	2.375AspAsp: 2.375 ± 0.058	3.974AspGlu: 3.974 ± 0.09	2.444AspPhe: 2.444 ± 0.056	4.478AspGly: 4.478 ± 0.099	0.991AspHis: 0.991 ± 0.04	3.209AspIle: 3.209 ± 0.063	2.702AspLys: 2.702 ± 0.062	4.984AspLeu: 4.984 ± 0.079	1.552AspMet: 1.552 ± 0.04	1.529AspAsn: 1.529 ± 0.045	2.388AspPro: 2.388 ± 0.057	1.668AspGln: 1.668 ± 0.046	2.575AspArg: 2.575 ± 0.059	2.722AspSer: 2.722 ± 0.064	3.004AspThr: 3.004 ± 0.062	3.465AspVal: 3.465 ± 0.07	0.62AspTrp: 0.62 ± 0.03	2.264AspTyr: 2.264 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.229GluAla: 5.229 ± 0.095	0.773GluCys: 0.773 ± 0.03	3.989GluAsp: 3.989 ± 0.073	7.818GluGlu: 7.818 ± 0.135	2.163GluPhe: 2.163 ± 0.049	5.665GluGly: 5.665 ± 0.099	1.392GluHis: 1.392 ± 0.04	4.188GluIle: 4.188 ± 0.073	6.325GluLys: 6.325 ± 0.105	6.406GluLeu: 6.406 ± 0.103	2.254GluMet: 2.254 ± 0.054	3.56GluAsn: 3.56 ± 0.06	2.365GluPro: 2.365 ± 0.065	3.614GluGln: 3.614 ± 0.088	3.507GluArg: 3.507 ± 0.074	3.274GluSer: 3.274 ± 0.062	3.535GluThr: 3.535 ± 0.067	4.138GluVal: 4.138 ± 0.08	0.68GluTrp: 0.68 ± 0.031	2.52GluTyr: 2.52 ± 0.065	0.0GluXaa: 0.0 ± 0.0
Phe	3.34PheAla: 3.34 ± 0.061	0.955PheCys: 0.955 ± 0.035	2.279PheAsp: 2.279 ± 0.055	2.222PheGlu: 2.222 ± 0.05	1.974PhePhe: 1.974 ± 0.059	2.898PheGly: 2.898 ± 0.078	0.876PheHis: 0.876 ± 0.035	1.86PheIle: 1.86 ± 0.049	1.283PheLys: 1.283 ± 0.037	4.204PheLeu: 4.204 ± 0.083	0.84PheMet: 0.84 ± 0.038	1.121PheAsn: 1.121 ± 0.04	1.681PhePro: 1.681 ± 0.048	1.642PheGln: 1.642 ± 0.043	1.99PheArg: 1.99 ± 0.054	3.186PheSer: 3.186 ± 0.066	2.254PheThr: 2.254 ± 0.055	2.651PheVal: 2.651 ± 0.052	0.506PheTrp: 0.506 ± 0.025	1.503PheTyr: 1.503 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	6.355GlyAla: 6.355 ± 0.116	1.462GlyCys: 1.462 ± 0.046	3.891GlyAsp: 3.891 ± 0.075	6.067GlyGlu: 6.067 ± 0.094	3.054GlyPhe: 3.054 ± 0.066	6.408GlyGly: 6.408 ± 0.14	1.491GlyHis: 1.491 ± 0.04	4.629GlyIle: 4.629 ± 0.084	5.286GlyLys: 5.286 ± 0.085	7.2GlyLeu: 7.2 ± 0.104	2.426GlyMet: 2.426 ± 0.053	2.631GlyAsn: 2.631 ± 0.062	2.017GlyPro: 2.017 ± 0.063	3.08GlyGln: 3.08 ± 0.065	4.069GlyArg: 4.069 ± 0.071	4.575GlySer: 4.575 ± 0.099	4.343GlyThr: 4.343 ± 0.099	5.885GlyVal: 5.885 ± 0.09	0.871GlyTrp: 0.871 ± 0.035	2.948GlyTyr: 2.948 ± 0.069	0.0GlyXaa: 0.0 ± 0.0
His	1.221HisAla: 1.221 ± 0.037	0.405HisCys: 0.405 ± 0.023	0.868HisAsp: 0.868 ± 0.035	1.001HisGlu: 1.001 ± 0.04	0.902HisPhe: 0.902 ± 0.032	1.478HisGly: 1.478 ± 0.049	0.464HisHis: 0.464 ± 0.028	1.306HisIle: 1.306 ± 0.045	0.838HisLys: 0.838 ± 0.032	1.835HisLeu: 1.835 ± 0.045	0.562HisMet: 0.562 ± 0.024	0.638HisAsn: 0.638 ± 0.027	1.107HisPro: 1.107 ± 0.039	0.739HisGln: 0.739 ± 0.028	1.165HisArg: 1.165 ± 0.038	1.079HisSer: 1.079 ± 0.035	1.107HisThr: 1.107 ± 0.036	1.01HisVal: 1.01 ± 0.036	0.238HisTrp: 0.238 ± 0.018	0.683HisTyr: 0.683 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	4.787IleAla: 4.787 ± 0.088	1.097IleCys: 1.097 ± 0.041	3.092IleAsp: 3.092 ± 0.071	3.068IleGlu: 3.068 ± 0.06	2.137IlePhe: 2.137 ± 0.057	4.008IleGly: 4.008 ± 0.077	1.167IleHis: 1.167 ± 0.039	2.898IleIle: 2.898 ± 0.067	2.467IleLys: 2.467 ± 0.051	5.768IleLeu: 5.768 ± 0.092	1.363IleMet: 1.363 ± 0.048	1.889IleAsn: 1.889 ± 0.053	2.874IlePro: 2.874 ± 0.058	2.243IleGln: 2.243 ± 0.059	3.359IleArg: 3.359 ± 0.064	3.699IleSer: 3.699 ± 0.064	3.616IleThr: 3.616 ± 0.08	3.655IleVal: 3.655 ± 0.081	0.517IleTrp: 0.517 ± 0.025	1.895IleTyr: 1.895 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	5.01LysAla: 5.01 ± 0.081	0.561LysCys: 0.561 ± 0.027	2.835LysAsp: 2.835 ± 0.063	4.935LysGlu: 4.935 ± 0.088	1.29LysPhe: 1.29 ± 0.04	4.002LysGly: 4.002 ± 0.08	0.72LysHis: 0.72 ± 0.03	3.024LysIle: 3.024 ± 0.069	4.324LysLys: 4.324 ± 0.081	4.641LysLeu: 4.641 ± 0.074	1.832LysMet: 1.832 ± 0.051	2.486LysAsn: 2.486 ± 0.06	1.964LysPro: 1.964 ± 0.053	2.098LysGln: 2.098 ± 0.051	2.985LysArg: 2.985 ± 0.067	2.538LysSer: 2.538 ± 0.063	3.023LysThr: 3.023 ± 0.063	3.41LysVal: 3.41 ± 0.072	0.542LysTrp: 0.542 ± 0.026	1.618LysTyr: 1.618 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	8.471LeuAla: 8.471 ± 0.111	2.346LeuCys: 2.346 ± 0.054	5.183LeuAsp: 5.183 ± 0.091	7.545LeuGlu: 7.545 ± 0.113	4.27LeuPhe: 4.27 ± 0.092	7.318LeuGly: 7.318 ± 0.119	1.782LeuHis: 1.782 ± 0.053	4.833LeuIle: 4.833 ± 0.09	4.597LeuLys: 4.597 ± 0.085	11.477LeuLeu: 11.477 ± 0.198	2.777LeuMet: 2.777 ± 0.06	2.93LeuAsn: 2.93 ± 0.056	5.017LeuPro: 5.017 ± 0.09	3.535LeuGln: 3.535 ± 0.068	5.831LeuArg: 5.831 ± 0.09	7.246LeuSer: 7.246 ± 0.107	5.703LeuThr: 5.703 ± 0.084	5.985LeuVal: 5.985 ± 0.089	1.163LeuTrp: 1.163 ± 0.035	3.281LeuTyr: 3.281 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.877MetAla: 2.877 ± 0.062	0.335MetCys: 0.335 ± 0.022	1.864MetAsp: 1.864 ± 0.047	2.782MetGlu: 2.782 ± 0.063	0.748MetPhe: 0.748 ± 0.029	2.474MetGly: 2.474 ± 0.062	0.397MetHis: 0.397 ± 0.022	1.464MetIle: 1.464 ± 0.045	2.01MetLys: 2.01 ± 0.046	2.754MetLeu: 2.754 ± 0.052	0.882MetMet: 0.882 ± 0.035	1.117MetAsn: 1.117 ± 0.037	1.224MetPro: 1.224 ± 0.034	1.001MetGln: 1.001 ± 0.038	1.372MetArg: 1.372 ± 0.039	1.492MetSer: 1.492 ± 0.038	1.64MetThr: 1.64 ± 0.042	1.947MetVal: 1.947 ± 0.049	0.16MetTrp: 0.16 ± 0.014	0.605MetTyr: 0.605 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.647AsnAla: 2.647 ± 0.06	0.531AsnCys: 0.531 ± 0.028	1.52AsnAsp: 1.52 ± 0.044	1.889AsnGlu: 1.889 ± 0.052	1.217AsnPhe: 1.217 ± 0.036	2.923AsnGly: 2.923 ± 0.071	0.604AsnHis: 0.604 ± 0.03	2.213AsnIle: 2.213 ± 0.045	1.604AsnLys: 1.604 ± 0.052	3.283AsnLeu: 3.283 ± 0.063	0.998AsnMet: 0.998 ± 0.033	1.111AsnAsn: 1.111 ± 0.045	1.857AsnPro: 1.857 ± 0.053	1.398AsnGln: 1.398 ± 0.042	1.869AsnArg: 1.869 ± 0.051	1.697AsnSer: 1.697 ± 0.051	1.877AsnThr: 1.877 ± 0.053	2.085AsnVal: 2.085 ± 0.057	0.352AsnTrp: 0.352 ± 0.021	1.212AsnTyr: 1.212 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	3.698ProAla: 3.698 ± 0.077	0.607ProCys: 0.607 ± 0.026	2.509ProAsp: 2.509 ± 0.057	4.08ProGlu: 4.08 ± 0.088	1.76ProPhe: 1.76 ± 0.051	3.308ProGly: 3.308 ± 0.069	0.826ProHis: 0.826 ± 0.031	1.997ProIle: 1.997 ± 0.056	1.77ProLys: 1.77 ± 0.047	4.039ProLeu: 4.039 ± 0.078	1.079ProMet: 1.079 ± 0.037	1.088ProAsn: 1.088 ± 0.04	1.63ProPro: 1.63 ± 0.052	2.272ProGln: 2.272 ± 0.063	1.939ProArg: 1.939 ± 0.046	2.469ProSer: 2.469 ± 0.049	1.968ProThr: 1.968 ± 0.055	3.621ProVal: 3.621 ± 0.068	0.484ProTrp: 0.484 ± 0.024	1.457ProTyr: 1.457 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	4.147GlnAla: 4.147 ± 0.087	0.49GlnCys: 0.49 ± 0.024	1.915GlnAsp: 1.915 ± 0.043	3.486GlnGlu: 3.486 ± 0.078	1.16GlnPhe: 1.16 ± 0.035	3.513GlnGly: 3.513 ± 0.076	0.622GlnHis: 0.622 ± 0.029	2.386GlnIle: 2.386 ± 0.061	2.937GlnLys: 2.937 ± 0.06	3.83GlnLeu: 3.83 ± 0.08	1.448GlnMet: 1.448 ± 0.043	1.752GlnAsn: 1.752 ± 0.039	1.586GlnPro: 1.586 ± 0.044	2.247GlnGln: 2.247 ± 0.066	2.298GlnArg: 2.298 ± 0.067	2.213GlnSer: 2.213 ± 0.059	1.955GlnThr: 1.955 ± 0.05	3.35GlnVal: 3.35 ± 0.07	0.553GlnTrp: 0.553 ± 0.028	1.309GlnTyr: 1.309 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	3.864ArgAla: 3.864 ± 0.076	0.915ArgCys: 0.915 ± 0.033	2.583ArgAsp: 2.583 ± 0.062	4.429ArgGlu: 4.429 ± 0.086	2.314ArgPhe: 2.314 ± 0.054	3.567ArgGly: 3.567 ± 0.067	1.086ArgHis: 1.086 ± 0.032	3.116ArgIle: 3.116 ± 0.062	3.158ArgLys: 3.158 ± 0.061	5.873ArgLeu: 5.873 ± 0.102	1.789ArgMet: 1.789 ± 0.046	1.665ArgAsn: 1.665 ± 0.044	2.168ArgPro: 2.168 ± 0.051	3.217ArgGln: 3.217 ± 0.072	3.942ArgArg: 3.942 ± 0.087	2.776ArgSer: 2.776 ± 0.051	2.508ArgThr: 2.508 ± 0.062	3.255ArgVal: 3.255 ± 0.061	0.636ArgTrp: 0.636 ± 0.027	1.965ArgTyr: 1.965 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.782SerAla: 4.782 ± 0.079	0.922SerCys: 0.922 ± 0.033	2.797SerAsp: 2.797 ± 0.071	2.891SerGlu: 2.891 ± 0.066	2.642SerPhe: 2.642 ± 0.064	5.041SerGly: 5.041 ± 0.103	1.173SerHis: 1.173 ± 0.04	3.257SerIle: 3.257 ± 0.078	2.301SerLys: 2.301 ± 0.056	6.115SerLeu: 6.115 ± 0.097	1.574SerMet: 1.574 ± 0.046	1.598SerAsn: 1.598 ± 0.047	2.776SerPro: 2.776 ± 0.06	2.938SerGln: 2.938 ± 0.06	3.486SerArg: 3.486 ± 0.063	3.572SerSer: 3.572 ± 0.091	2.673SerThr: 2.673 ± 0.06	3.793SerVal: 3.793 ± 0.064	0.645SerTrp: 0.645 ± 0.03	2.104SerTyr: 2.104 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	4.997ThrAla: 4.997 ± 0.094	0.704ThrCys: 0.704 ± 0.026	2.817ThrAsp: 2.817 ± 0.061	3.146ThrGlu: 3.146 ± 0.059	2.024ThrPhe: 2.024 ± 0.053	4.843ThrGly: 4.843 ± 0.085	1.019ThrHis: 1.019 ± 0.032	3.092ThrIle: 3.092 ± 0.078	2.127ThrLys: 2.127 ± 0.057	5.705ThrLeu: 5.705 ± 0.098	1.436ThrMet: 1.436 ± 0.038	1.445ThrAsn: 1.445 ± 0.051	2.864ThrPro: 2.864 ± 0.059	1.987ThrGln: 1.987 ± 0.051	2.512ThrArg: 2.512 ± 0.053	2.564ThrSer: 2.564 ± 0.066	2.532ThrThr: 2.532 ± 0.068	4.511ThrVal: 4.511 ± 0.108	0.521ThrTrp: 0.521 ± 0.025	1.73ThrTyr: 1.73 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	5.159ValAla: 5.159 ± 0.093	1.347ValCys: 1.347 ± 0.046	3.684ValAsp: 3.684 ± 0.074	4.878ValGlu: 4.878 ± 0.093	2.909ValPhe: 2.909 ± 0.061	4.48ValGly: 4.48 ± 0.077	1.187ValHis: 1.187 ± 0.043	3.97ValIle: 3.97 ± 0.076	3.375ValLys: 3.375 ± 0.078	7.234ValLeu: 7.234 ± 0.1	2.036ValMet: 2.036 ± 0.051	2.134ValAsn: 2.134 ± 0.056	2.862ValPro: 2.862 ± 0.061	2.38ValGln: 2.38 ± 0.051	3.568ValArg: 3.568 ± 0.073	4.541ValSer: 4.541 ± 0.096	4.248ValThr: 4.248 ± 0.094	4.789ValVal: 4.789 ± 0.077	0.685ValTrp: 0.685 ± 0.031	2.331ValTyr: 2.331 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.848TrpAla: 0.848 ± 0.036	0.178TrpCys: 0.178 ± 0.014	0.681TrpAsp: 0.681 ± 0.029	0.846TrpGlu: 0.846 ± 0.033	0.411TrpPhe: 0.411 ± 0.022	0.893TrpGly: 0.893 ± 0.035	0.257TrpHis: 0.257 ± 0.016	0.478TrpIle: 0.478 ± 0.022	0.668TrpLys: 0.668 ± 0.03	1.034TrpLeu: 1.034 ± 0.041	0.316TrpMet: 0.316 ± 0.018	0.393TrpAsn: 0.393 ± 0.022	0.341TrpPro: 0.341 ± 0.02	0.547TrpGln: 0.547 ± 0.026	0.6TrpArg: 0.6 ± 0.029	0.514TrpSer: 0.514 ± 0.027	0.42TrpThr: 0.42 ± 0.023	0.651TrpVal: 0.651 ± 0.029	0.112TrpTrp: 0.112 ± 0.013	0.382TrpTyr: 0.382 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.675TyrAla: 2.675 ± 0.055	0.613TyrCys: 0.613 ± 0.023	2.141TyrAsp: 2.141 ± 0.048	2.307TyrGlu: 2.307 ± 0.062	1.595TyrPhe: 1.595 ± 0.044	2.814TyrGly: 2.814 ± 0.059	0.719TyrHis: 0.719 ± 0.03	1.864TyrIle: 1.864 ± 0.042	1.417TyrLys: 1.417 ± 0.04	3.446TyrLeu: 3.446 ± 0.068	0.786TyrMet: 0.786 ± 0.032	1.235TyrAsn: 1.235 ± 0.041	1.374TyrPro: 1.374 ± 0.042	1.525TyrGln: 1.525 ± 0.045	1.953TyrArg: 1.953 ± 0.05	1.897TyrSer: 1.897 ± 0.047	2.073TyrThr: 2.073 ± 0.061	2.109TyrVal: 2.109 ± 0.056	0.322TyrTrp: 0.322 ± 0.02	1.422TyrTyr: 1.422 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2802 proteins (841265 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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